前列腺癌组织中TMPRSS2基因与ETS家族基因的多种融合亚型及其意义

目的 了解前列腺癌组织标本中跨膜丝氨酸蛋白酶2(TMPRSS2)基因与ETS转录因子家族成员ETS相关基因(ERG)、ETS变异体1(ETV1)及ETS变异体4(ETV4)基因之间的融合情况及意义.方法 采用巢式逆转录聚合酶链反应(RT-PCR)琼脂糖凝胶电泳法检测32例前列腺癌患者及34例前列腺良性增生患者前列腺组织中TMPRSS2基因与ETS家族基因融合的TMPRSS2/ERG、TMPRSS2/ETV1、TMPRSS2/ETV4转录体;琼脂糖凝胶电泳阳性者,纯化PCR产物进行直接测序,用BLAST在线软件比对确定融合位点;分析融合基因与Gleason分级关系.结果 在32例前列腺癌患者组织标本中,检测到TMPRSS2/ERG融合基因17例(53.1%),含5种不同融合基因亚型,其中1种为新发现融合基因亚型(GenBank登录号:EU090248),单一标本中可检测到1种以上TMPRSS2/ERG融合基因亚型;检测到TMPRSS2/ETV1融合基因2例(6.3%),为新发现融合基因亚型(GenBank 登录号:EU090249);未检到TMPRSS2/ETV4融合基因型.34例前列腺良性增生组织标本中均未检测到TMPRSS2/ERG、TMPRSS2/ETV1和TMPRSS2/ETV4融合基因型.按Gleason评分值分为中分化与低分化的两组前列腺癌组织标本之间融合基因阳性率差异无统计学意义(P=0.169).结论 前列腺癌组织中存在TMPRSS2/ERG和TMPRSS2/ETV1融合基因及多种亚型;前列腺癌融合基因的发现有望为前列腺癌的发病机制研究提供新的思路.     

推拿松肌及整骨手法相结合治疗腰椎间盘突出症77例

[目的]总结推拿松肌及整骨手法相结合治疗腰椎间盘突出症的临床疗效.[方法]运用松肌及整骨手法相结合的非手术方法治疗腰椎间盘突出症77例.[结果]77例腰椎间盘突出症患者中,痊愈57例,显效16例,无效4例,总有效率达94.81%.[结论]以推拿松肌及整骨手法相结合治疗腰椎间盘突出症,疗效满意.     

多层螺旋CT重建技术在颌面部骨折的临床应用

目的探讨多层螺旋CT多平面重建（MPR）、容积重现（vR）和最大密度投影重建（MIV）在颌面部骨折中的临床应用价值。方法对96例颌面部创伤患者进行多层螺旋CT横断位容积扫描，常规层厚2mm，重建间隔1mm，螺距0．9mm,原始图像传输至工作站进行MPR、VR、MIP三维重建。结果三维重建影像显示骨折范围、位置、类型、骨折线走行方向及碎骨片移位等信息方面较轴位扫描图像有显著优势。结论多层螺旋CT三维重建技术的应用对颌面部骨折显示良好，有利于临床制定治疗方案。     

Effects of armepavine against hepatic fibrosis induced by thioacetamide in rats

The aim of this study was to investigate if armepavine (Arm, C₁₉H₂₃O₃N) could exert inhibitory effects against hepatic fibrosis in rats. A cell line of rat hepatic stellate cells (HSC‐T6) was stimulated with tumour necrosis factor‐α (TNF‐α) to evaluate the inhibitory effects of Arm. Rats were injected with thioacetamide (TAA; 300 mg/kg, intraperitoneally) thrice a week for 4 weeks to induce hepatic fibrosis, with Arm (3 or 10 mg/kg) given by gavage twice a day. Liver sections were taken for western blotting, fibrosis scoring and immunofluorescence staining. Arm (1–10 µm ) concentration‐dependently attenuated TNF‐α‐stimulated: (i) protein expressions of α‐smooth muscle actin (α‐SMA), collagen type I and angiopoietin‐1; (ii) H₂O₂ production; and (iii) NF‐κB, JunD and C/EBPß (cytidine‐cytidine‐adenosine‐adenosine‐thymidine (CCAAT)/enhancer binding protein‐ß (EBPß)) nuclear translocations in HSC‐T6 cells. In vivo Arm treatment significantly reduced plasma aspartate transaminase and alanine transaminase levels, hepatic α‐SMA expression and collagen contents, and fibrosis scores of TAA‐injected rats. Moreover, Arm treatment decreased α‐SMA‐ and NF‐κB‐positive cells in immunohistochemical staining, and mRNA expression levels of IL‐6, TGF‐ß1, TIMP‐1, col1α2, iNOS and ICAM‐1 genes, but up‐regulated the metallothionein gene in the livers of TAA‐injected rats. Our results indicated that Arm exerted both in vitro and in vivo antifibrotic effects in rats, with inhibition of NF‐κB, JunD and C/EBPß pathways. Copyright © 2011 John Wiley & Sons, Ltd.     

鼻分泌物及皮肤组织中麻风菌及其PGL-1抗原的检测

为了更好地理解麻风菌鼻携带在麻风病传播、维持中的作用，以及运用鼻携带检测来评价麻风病防治效果，比较了PCR和Dot-ELISA／ECL平行检测32例活动性麻风患者、13例愈后者和143名麻风家内接触者鼻分泌物及皮肤组织中的麻风菌及其PGL-1抗原。结果显示，Dot-ELISA／ECL具有较好的敏感性、特异性，是一项适用于现场研究的简便、快速、经济的麻风流行病学工具。此外，用于免疫学试验，GVHP是一种吸附性好，适合于检测粘膜分泌物抗原的载体。     

伴双手麻木的结节性皮肤淀粉样变一例

患者男,53岁,因面部、双手、阴茎、臀部结节斑块5年就诊于福州市皮肤病防治院皮肤科。5年前无明显诱因患者前额出现数个粟粒至蚕豆大小结节,因无自觉症状,未予治疗。皮疹逐渐增多、增大,双上眼睑、鼻背、双手指、阴茎、臀部等部位逐渐出现类似皮疹,伴双手麻木感,呈阵发性,与天气冷热无关,无发热、四肢乏力、关节肿痛等不适,曾自行外用多种药膏（具体不详）无效。既往史及家族史无特殊,各系统检查未见明显异常……     

Consensus guidelines for the management of atopic dermatitis: An Asia–Pacific perspective

Atopic dermatitis (AD) is a relatively common disease in patients in the Asia–Pacific region. It presents a particular clinical challenge and requires careful clinical management. The chronic nature of AD characterized by flares, exacerbations and periods of quiescence requires a multipronged approach aimed at reducing itch, inflammation and the appearance of secondary lesions. In addition, varying levels of maintenance therapy may be required to avoid exacerbations. Survey data from the region indicate that there is significant variation across the Asia–Pacific with regard to current treatment practices. The management of AD may also be influenced by differing health‐care systems, variable climate, access to medical care and cultural diversity. The current consensus guidelines have been developed to provide up‐to‐date and concise evidence‐ and experience‐based recommendations directed towards general practitioners and general dermatologists in the Asia–Pacific region on the management of pediatric and adult AD.     

基于知识图谱构建和定性访谈法探析张忠德教授辨治间质性肺病临床特征与方药规律＊

目的 构建个性化知识图谱技术和定性访谈法结合，进一步挖掘张忠德教授辨治间质性肺疾病临证特征与用药规律。方法 采用回顾性分析，系统收集张忠德教授广东省中医院门诊2010年8月至2020年8月治疗间质性肺疾病病历，按照诊断标准、纳入标准、排除标准，严格筛选后，通过广东省中医院大数据挖掘团队中医药大数据智能处理与知识服务系统进行数据挖掘分析，并通过多元化视觉定性访谈法，将定量与定性分析有效结合。结果 共筛选出347首方，共141味药物，常用药物频次 ≥ 84次的药物有10味，其中党参、麦芽、黄芪、紫菀、白术等为核心用药，通过症状与药物推理知识地图显示，党参、炒麦芽、黄芪、大枣、太子参、山萸肉、巴戟天等为主要治疗用药；临证遣方用药知识关联分析，得知咳嗽、耳鸣、心悸、水肿、头痛、胸闷、恶寒等为多见，针对咳嗽，首选紫苏子、橘红、桂枝等温肺降气通阳之品等；频繁聚集显示，常用药对炒麦芽-炒白术、黄芪-党参、黄精-菟丝子、前胡-紫菀、炙枇杷叶-浙贝母等；聚类分析结果得到4组关系密切的聚类新药物组合；以脾为中轴，肺肾共扶为主的“平调五脏论”，分期阶梯辨治间质性肺疾病。结论 张忠德教授认为间质性肺疾病，虚实夹杂为多见，应从肺、脾、肾着手，采用平调五脏论分期阶梯辨治，用药配伍精简，以和为贵，以平为期。     

Sealing of anodized magnesium alloy AZ31 with MgAl layered double hydroxides layers

In this work, anodized magnesium alloy AZ31 with and without boiling water sealing was pre-prepared, and then MgAl-layered double hydroxide (LDH) films were fabricated on it through hydrothermal chemical conversion of the pre-prepared anodic layer. The morphology, structure, and composition of the films were characterized by XRD, SEM, EDS, FT-IR, XPS and GDOES. It was found that the porosity of the films was reduced after in situ fabrication of the LDHs. The effects of boiling water sealing treatment on the anodized substrate were also discussed. Moreover, the polarization curve, EIS, and immersion tests showed that LDHs fabricated on the anodized substrate with boiling water sealing treatment exhibited a significant long period of protection for the substrate.

In this work anodized magnesium alloy AZ31 with and without boiling water sealing was pre-prepared, and then MgAl-layered double hydroxide (LDH) films were fabricated on it through hydrothermal chemical conversion of the pre-prepared anodic layer.