High expression levels of IKK alpha and IKK beta are necessary for the malignant properties of liver cancer

IKK-NF-kappa B signaling is regarded as an important factor in hepatocarcinogenesis and a potential target for liver cancer therapy.Therefore,in this study,we analyzed the expression of mRNAs encoding components and targets of NF-kappa B signaling including IKK alpha,IKK beta,RANK,RANKL,OPG,CyclinD3,mammary serine protease inhibitor(Maspin),CyclinD1,c-FLIP,Bcl-x1,Stat3,Cip1 and Cip2 by real-time PCR in 40 patients with liver cancer.After statistical analysis,7 indices including IKK alpha,IKK beta,RANK,Maspi...     

腹部巨大囊性病变CT分析

1临床资料 腹部巨大囊性病变（最小径线10cm以上者）28（男16,女12）例,年龄0.5～83（平均44.6）岁,腹痛14例,腹胀10例,腰痛9例,发热2例,病程1mo至10数年。良性26例：卵巢囊肿4例,卵巢囊腺瘤2例,肾囊肿2例,肾重度积水3例,输尿管囊肿2例,胰腺及脾脏囊肿各3例。     

A possible autocrine role for interleukin-6 in two lymphoma cell lines

Interleukin-6 (IL-6) is a growth factor with diverse biologic activity. Originally described as a T-cell product that enhances immunoglobulin (Ig) secretion in antigen-stimulated B cells, it also affects the growth of T cells, plasmacytomas, hybridomas, and hematopoietic stem cells. We report the expression and secretion of IL-6 by two lymphoma cell lines, OCI-LY3 and OCI-LY12. Addition of recombinant IL-6 stimulated their growth, whereas addition of polyclonal anti- recombinant IL-6 (anti-rIL-6) had a marked inhibitory effect on proliferation. These results suggest an autocrine role for IL-6 in the growth of these lymphoma cells in culture.     

A novel mutation L619F in the cardiac Na+ channel SCN5A associated with long-QT syndrome (LQT3): a role for the I-II linker in inactivation gating

Congenital long QT syndrome type 3 (LQT3) is caused by mutations in the gene SCN5A encoding the alpha-subunit of the cardiac Na(+) channel (Nav1.5). Functional studies of SCN5A mutations in the linker between domains III and IV, and more recently the C-terminus, have been shown to alter inactivation gating. Here we report a novel LQT3 mutation, L619F (LF), located in the domain I-II linker. In an infant with prolonged QTc intervals, mutational analysis identified a heterozygous missense mutation (L619F) in the domain I-II linker of the cardiac Na(+) channel. Wild-type (WT) and mutant channels were studied by whole-cell patch-clamp analysis in transiently expressed HEK cells. LF channels increase maintained Na(+) current (0.79 pA/pF for LF; 0.26 pA/pF for WT) during prolonged depolarization. We found a +5.8mV shift in steady state inactivation in LF channels compared to WT (WT, V(1/2)=-64.0 mV; LF, V(1/2)=-58.2 mV). The positive shift of inactivation, without a corresponding shift in activation, increases the overlap window current in LF relative to WT (1.09 vs. 0.58 pA/pF), as measured using a positive voltage ramp protocol (-100 to +50 mV in 2s). The increase in window current, combined with an increase in non-inactivating Na(+) current, may act to prolong the AP plateau and is consistent with the disease phenotype observed in patients. Moreover, the defective inactivation imposed by the L619F mutation implies a role for the I-II linker in the Na(+) channel inactivation process.     

EMD56431对犬冠脉和肠系膜动脉的作用

目的：研究ＥＭＤ对犬冠脉和肠系膜动脉的松弛作用。方法：用２０和６０ｍｍｏｌ／Ｌ ＫＣｌ激动冠脉（＋Ｅ,－Ｅ）,观察ＥＭＤ的松弛效应。同时观察格列本脲对ＥＭＤ松弛冠脉的影响及ＥＭＤ对ＫＣｌ量效曲线的改变。此外,还测定了ＥＭＤ松驰犬肠系膜动脉和冠脉的ＥＣ５０。结果：０．７μｍｏｌ／Ｌ ＥＭＤ对２０ｍｍｏｌ／Ｌ ＫＣｌ去极化冠脉无效;格列本脲能有效拮抗ＥＭＤ对冠脉的松驰作用;ＥＭＤ使５～３０ｍｍｏｌ／Ｌ     

4-O-卤代酰基-4′-去甲基表鬼臼毒素类似物的合成与抗肿瘤活性

为考察4′-去甲表鬼臼毒素C₄位上联结含卤素原子的酯化侧链时对化合物抗肿瘤活性的影响,设计并采用选择性酯化方法合成了9个新的4′-去甲基表鬼臼毒素酯化产物。其中标题化合物在L₁₂₁₀白血病肿瘤细胞与KB细胞的体外生长抑制试验中普遍表现出显著的抑制活性,大部分化合物活性超过依托泊甙。而普通脂酸酯的活性较弱。     

离子对反相高效液相色谱法测定小柴胡胶囊制剂中黄芩苷的含量

目的：研究了离子对－反相高效液相色谱法测定小柴胡胶囊制剂中黄芩苷的含量。方法：用５０％甲醇作溶剂、超声波振荡法提取样品。色谱条件用μ－ＢｏｎｄｐａｋＣ１８作分析柱,ＷａｔｅｒｓＧｕａｒｄ－ＰＡＫＣ１８作保护柱,水相（取庚烷碘酸钠０．４０ｇ,磷酸二氢钠３．９０ｇ冰醛酸２０ｍｌ,加水溶解并稀释至１０００ｍｌ）＝甲醇（５６：４４）作流动相,流速１ｍｌ／ｍｉｎ,ＵＶ检测波长为２７５ｎｍ。结果：黄芩苷与 份     

从D-葡萄糖合成(3S,4S,5R)-4-O-苄基-3-表莽草酸甲酯

本文报道用D-葡萄糖为原料,经九步反应,对映选择地合成了标题化合物(10),从而为制备新抗生素二(口恶)霉素及类似物提供了对映选择合成的途径。     

曲马多术后镇痛不同给药途径的临床研究

目的：探讨曲马多在硬膜外腔给药与静脉给药术后镇痛效果同芬太尼静脉镇痛的比较。方法：75例行腹部手术病人,随机分为TE组（n=25,硬膜外腔曲马多组）,FI组（n=25,静脉曲马多组）,FI组（n=25,静脉芬太尼组）;采用雅培（Mbbot13960－36）泵,选择持续剂量＋PCA给药模式,每例病人在关腹时给予负荷剂量。结果：（1）TE组与TI组的4,24和48h肜药量,按压次数,D／D比值（按压次数／实际给药次数）、VAS评分均相似,P＞0．05,TE组与FI组,TI组与FI组相比用药量有明显差异,P＜0．05,VAS评分无差异。（2）并发症：恶心发生率TE组12％,TI组8％,FI组12％,呕吐发生率TE组4％,TI组4％,FI组8％,呼吸抑制发生率FI组4％。结论：曲马多是一种安全有效的术后镇痛药,硬膜外腔给药与静脉给药术后镇痛效果无差别。