大学生心理健康教育干预研究

目的　研究高校心理健康干预模式 ,提高大学生心理健康水平。方法　采取分层整群抽样的方法 ,运用SCL - 90自评量表对该院学生测试评分 ,分别进行实施心理健康干预模式 (心理教育、心理预防、心理训练 )前后状况调查 ,比较学生心理测试总体水平。结果　学校心理健康教育干预实施后一年 ,学生心理测试 8项因子分均值低于心理健康干预实施前 ,其中强迫、人际关系、焦虑、恐怖、偏执因子分实施前后有显著性差异 (P <0 0 5 )。结论　学校实施心理健康教育干预能有效地维护大学生心理健康 ,促进大学生心理发展 ,提高大学生心理素质     

Characteristics of deoxycholic acid-induced histamine release from mast cells of guinea-pig rectocolonic mucosa and rat peritoneal cavity

Deoxycholic acid (DA) caused a dose-related release of histamine (HR) from mast cells of rat peritoneum (RPMC) and mucosal cells of guinea pig rectocolon (RCMC). In both cell populations, DA-induced HR was: (1) accompared by a parallel release of lactate dehydrogenase (LDH), (2) not affected by metabolic inhibitors, (3) dependent on time of incubation, temperature and pH, and affected by Ca⁺⁺ concentration in RPMC but not in RCMC. DA-induced HR from RCMC may be involved in certain functional disorders of the colon.     

香菇多糖注射液对慢性乙型肝炎外周血单个核细胞膜IL—2受体表达的影响

The effects of lentinan on peripheral blood mononuclear cell (PBMC) expression of IL-2 receptor in patients with chronic hepatitis B in vitro and in vivo were studied. The expression percentage of IL-2 receptor in 44 patients with chronic hepatitis before treatment was 36.74 +/- 7.74, which was lower than healthy subjects, and was 48.92 +/- 3.79 after 2 months treatment of lentinan, which was approximated the normal control. PBMC from 30 patients with chronic hepatitis B were cultured in vitro with or without lentinan. The percentage of expression IL-2 receptor was 12.81 +/- 2.62 without lentinan and was 35.70 +/- 4.87 in the presence of lentinan. The concentration of lentinan stimulating expression of IL-2 receptor was between 0.2 to 200 g.ml-1. The results suggest that lentinan stimulates expression of IL-2 receptor on PBMC, which is associated with the therapeutic effectiveness of lentinan treatment.     

556例遗传咨询病例细胞遗传学分析

目的 :分析染色体异常与临床疾病的关系。方法 :应用细胞培养染色体分析技术对 5 5 6例遗传咨询病例进行细胞遗传学检查。结果 :发现 47例染色体结构或数目异常 ,占受检人数的 8.4% ,其中常染色体异常 39例 ,性染色体异常 8例。此外检出 46例 ,XY女性反转综合征 2例。结论 :反复自然流产和不孕不育夫妇及怀疑 Down综合征者应进行染色体检查     

PTCA及支架植入术治疗心肌梗死并重症泵功能衰竭近期效果观察

OBJECTIVE: To evaluate the short-term therapeutic effect of percutaneous transluminal coronary angioplasty (PTCA) with stent implantation in the treatment of myocardial infarction with severe pump failure. METHODS: The clinical data of 73 patients receiving PTCA and stent implantation for myocardial infarction with severity pump failure were analyzed and grouped according the occurrence of complications, degree of the vascular lesions and the complexity of the surgical procedures. Preoperative and postoperative ventricular ejection fractions (LVEF) were compared in each case. RESULTS: The degree of vascular lesions and surgical complexity were not shown to relate to the occurrence of the complications. Except in cases complicated by chronic renal dysfunction, significant improvement was achieved in the patients 7 d after the operation (P<0.05), regardless of different degrees of vascular lesions and surgical complexity. CONCLUSION: PTCA with stent implantation is effective to improve the short-term cardiac function of patients with myocardial infarction and severe pump failure.     

钚-239对大鼠胸腺淋巴细胞超微结构的影响

本文观察了硝酸钚溶液经腹腔一次注入大鼠体内,不同时间后胸腺淋巴细胞超徽结构损伤效应与修复规律.早期可见淋巴细胞质膜及内膜系统的变化,以后出现核损伤,注后20天损伤最严重。以小淋巴细胞的损伤更明显。注后1月细胞损伤有所减轻,6个月基本恢复正常。     

Influence of TAT-peptide polymerization on properties and transfection activity of TAT/DNA polyplexes.

Use of bioactive cationic peptides as gene carriers is limited by instability of their DNA complexes in vivo and by the loss of their biological activity due to undesired interactions of their bioactive parts with the DNA. To overcome the two major limitations, biodegradable high-molecular-weight form of TAT peptide (POLYTAT) sensitive to cellular redox-potential gradients was synthesized in this study by oxidative polycondensation. Physicochemical and transfection properties of DNA polyplexes based on POLYTAT were investigated and compared with polyplexes based on TAT polymer prepared by in situ template-assisted polymerization. Physicochemical properties of TAT-based polyplexes were affected by the molecular weight and method of polymerization of the TAT peptide. All TAT-based DNA polyplexes exhibited reduced cytotoxicity when compared with control polyethylenimine (PEI) polyplexes. Polyplexes based on both high-molecular-weight TAT polypeptides exhibited increased transfection efficiency compared to control TAT peptide but lower than that of PEI polyplexes. The evidence shows that transfection activity of TAT-based polyplexes is strongly dependent on the presence of chloroquine and therefore suggests that TAT polyplexes are internalized by an endocytosis. Overall, high-molecular-weight reducible polycations based on bioactive peptides has the potential as versatile carriers of nucleic acids that display low cytotoxicity and can prove to be especially beneficial in cases that require surface presentation of membrane-active or cell-specific targeting peptides.     

MED术中应用自制器械处理后纵韧带钙化及骨赘增生

目的探讨脊柱后路显微内镜(microendoscopic disectomy,MED)手术治疗腰椎间盘突出症合并后纵韧带钙化及椎体后缘骨赘增生的方法. 方法 MED手术中应用自制弧形纤维环刀和L形打入器治疗115例该类患者. 结果全组随访12～30个月,平均22个月,按MacNab标准:优、良109例,可5例,差1例,优良率94.8(109/115).未出现硬脊膜破裂及神经孙上,未发生椎间隙感染,无中转开放手术. 结论正确运用MED技术,配合采用自制器械及相应的改良方法,能较好地处理合并后纵韧带钙化及椎体后缘骨赘增生的难题,进一步扩大了手术适应证范围,提高了疗效及安全性.     

高职高专学生学业不良的成因及矫正对策

相对于普通高校，高职高专学校中学业不良学生的比例更高，管理的难度更大。因此，分析、研究学生学业不良的成因，并采取科学有效的措施予以矫正，对于促进学生健康成长，全面提高教育教学质量，具有积极的意义。     

老年性痴呆的病理特征与防治对策

老年痴呆症的发生与AB以及Tau蛋白的错误折叠密切相关，是蛋白质折叠相关的疾病，早期诊断是预防老年痴呆症的首要任务，而综合治疗是人类对抗老年痴呆症的必然举措。