全文获取类型
收费全文 | 350篇 |
免费 | 17篇 |
国内免费 | 51篇 |
专业分类
儿科学 | 13篇 |
基础医学 | 19篇 |
口腔科学 | 11篇 |
临床医学 | 81篇 |
内科学 | 92篇 |
皮肤病学 | 3篇 |
神经病学 | 2篇 |
特种医学 | 83篇 |
外科学 | 17篇 |
综合类 | 22篇 |
预防医学 | 16篇 |
眼科学 | 6篇 |
药学 | 35篇 |
肿瘤学 | 18篇 |
出版年
2022年 | 1篇 |
2021年 | 4篇 |
2020年 | 2篇 |
2019年 | 3篇 |
2018年 | 1篇 |
2017年 | 6篇 |
2016年 | 12篇 |
2015年 | 7篇 |
2014年 | 11篇 |
2013年 | 10篇 |
2012年 | 2篇 |
2011年 | 5篇 |
2010年 | 11篇 |
2009年 | 7篇 |
2008年 | 7篇 |
2007年 | 30篇 |
2006年 | 4篇 |
2005年 | 8篇 |
2004年 | 2篇 |
2003年 | 2篇 |
2002年 | 5篇 |
2001年 | 11篇 |
2000年 | 3篇 |
1999年 | 10篇 |
1998年 | 26篇 |
1997年 | 29篇 |
1996年 | 16篇 |
1995年 | 22篇 |
1994年 | 13篇 |
1993年 | 16篇 |
1992年 | 4篇 |
1991年 | 8篇 |
1990年 | 5篇 |
1989年 | 13篇 |
1988年 | 13篇 |
1987年 | 7篇 |
1986年 | 12篇 |
1985年 | 11篇 |
1984年 | 10篇 |
1983年 | 1篇 |
1982年 | 7篇 |
1981年 | 7篇 |
1980年 | 5篇 |
1979年 | 6篇 |
1977年 | 7篇 |
1976年 | 8篇 |
1975年 | 4篇 |
1970年 | 1篇 |
1968年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有418条查询结果,搜索用时 15 毫秒
71.
72.
Enzmann DR; Wheat R; Marshall WH; Bird R; Murphy-Irwin K; Karbon K; Hanbery J; Silverberg GD; Britt RH; Shuer L 《Radiology》1985,154(2):393-399
Intraoperative ultrasound (US) was compared to computed tomography (CT) in 41 intracranial and 6 spinal cord tumors. The studies correlated closely except for primary gliomas. Eight of the 22 primary intracranial gliomas (37%), including 1 low-grade and 7 anaplastic tumors, were larger and more extensive on US than on CT. Margins of non-enhanced primary astrocytomas were shown by US but not CT. Four anaplastic tumors (19%) exhibited echogenicity extending beyond the enhanced area. In 4 patients an enhanced lesion contained a lucent center which proved to be echogenic. Low-grade astrocytomas were relatively homogeneous on US, while anaplastic astrocytomas were more inhomogeneous. Cysts could be found in both types of astrocytomas and were often small and multiple. The echo pattern was not helpful in differentiating metastases from primary tumors, although all of them had sharp margins. Sonography of the central nervous system can provide valuable information about tumor morphology and margins. 相似文献
73.
Pulkkinen L; Smith FJ; Shimizu H; Murata S; Yaoita H; Hachisuka H; Nishikawa T; McLean WH; Uitto J 《Human molecular genetics》1996,5(10):1539-1546
In a distinct autosomal recessive variant of epidermolysis bullosa, EB- MD,
life-long skin blistering is associated with late-onset muscular dystrophy
of unknown etiology. Electron microscopy of these patients' skin suggests
that tissue separation occurs intracellularly at the level of the
hemidesmosomal inner plaque, which contains plectin, a high molecular
weight cytoskeletal associated protein, also expressed in the sarcolemma of
the muscle. In this study, we report two patients with EB-MD, each with a
homozygous deletion mutation in the plectin gene, PLEC1. In the first case,
the proband and her similarly affected sister had a homozygous 9 bp
deletion mutation, designated as 2719de19, which resulted in elimination of
three amino acids, QEA, in a sequence of 23 amino acids entirely conserved
between the mouse and human sequences. The proband in the second family
demonstrated a single nucleotide deletion at position 5866, designated as
5866delC, which resulted in frameshift and a premature termination codon
for translation 16 bp downstream from the site of deletion. The absence of
plectin in the hemidesmosomes, as reflected by negative immunofluorescence
with an anti-plectin antibody (HD-1), associated with fragility of basal
keratinocytes, implicates plectin as critical for binding of intermediate
keratin filament network to hemidesmosomal complexes. The function of
plectin as a putative attachment protein also in the muscle would explain
the clinical phenotype consisting of cutaneous fragility and muscular
dystrophy in EB-MD.
相似文献
74.
Molecular evidence that in situ-transduced fetal liver hematopoietic stem/progenitor cells give rise to medullary hematopoiesis in adult rats 总被引:4,自引:2,他引:2
We exploited the ability to transduce fetal liver hematopoietic stem/progenitor cells in situ with recombinant retrovirus, together with the ability to analyze proviral integration patterns into chromosomal DNA, to detect the cellular and organ fate of hematopoietic stem and progenitor-derived progeny in tissues and in the circulation of neonatal and adult rats. Two hundred seventeen fetuses were injected with retrovirus supernatant on day 16 of gestation, before the development of the bone marrow cavity. The progeny of 41 stem and progenitor cells from 97 liveborn rats were clonally identified. Pluripotent and lineage-restricted stem/progenitor clones derived from the fetal liver consistently gave rise to progeny in the marrow of newborn and adult rats. Patterns of differentiation of transduced stem and progenitor cells fell into distinct subsets. Blood cells derived from in situ transduced cells that originated in the fetal liver circulated throughout the life span of the adult animals. These data provide molecular evidence of the origin of medullary cavity hematopoiesis by cells derived from the fetal liver that were transduced in vivo, homed to the developing medullary cavity and proliferated in a normal medullary hematopoietic microenvironment. 相似文献
75.
A human monoclonal antibody specific for the leucine-33 (P1A1, HPA-1a) form of platelet glycoprotein IIIa from a V gene phage display library 总被引:3,自引:2,他引:1
IgG alloantibodies to polymorphic platelet glycoproteins (GPs) are known to be responsible for severe thrombocytopenia in the neonate and after transfusion. Platelet GPIIIa can have either a leucine or a proline at residue 33. The most immunogenic platelet alloantigen in thrombocytopenia is the leucine 33 form of GPIIIa. Here, we have generated human monoclonal antibody fragments that are specific for the leucine and not the proline form of GPIIIa and can inhibit the binding of polyclonal human IgG alloantibodies to GPIIIa leucine 33 on platelets. The antibody fragments were selected from a library of single chain Fv fragments displayed on the surface of filamentous phage. The VH gene repertoire was derived from the peripheral blood lymphocytes of an alloimmunized individual and recombined with a VL gene repertoire from a nonimmune source. Antibodies such as these, which are able to distinguish between two variant forms of a native antigen and which have been unobtainable by conventional hybridoma technology, have both diagnostic and potential therapeutic applications. 相似文献
76.
77.
Two adults with bizarre parosteal osteochondromatous proliferation of the hands and feet are presented. The pre-operative diagnosis may be difficult to make and the correct diagnosis was not made in either case until pathological examination of recurrent tumour masses occurred. The radiological and pathological features of this lesion are discussed. The diagnostic features which differentiate this condition from other causes of new bone formation in the peripheries are described. 相似文献
78.
To investigate the sodium magnetic resonance (MR) imaging characteristics of acute vasogenic edema, an experimental canine model was developed. Vasogenic edema was produced in the hemisphere of the dogs by the intraarterial infusion of hypertonic mannitol (25%). This solution opens the blood-brain barrier, allowing the influx of water, electrolytes, and proteins into the brain. The main advantage of this model over the established "cold injury" model is the lack of associated brain necrosis. Two patients with chronic vasogenic edema secondary to well-circumscribed meningiomas also underwent MR imaging. The sodium signal was markedly elevated in both clinical and experimental studies of vasogenic edema fluid compared with signal in healthy brain tissue. Extracellular sodium associated with vasogenic edema displayed MR imaging characteristics similar to that of sodium in serum. There was a trend toward a shortened T2 in edema fluid secondary to the presence of serum macromolecules. 相似文献
79.
Increased colonic wall thickness has been reported in patients exposed to large doses of high strength pancreatic enzyme preparations who did not develop fibrosing colonopathy. This has been interpreted as evidence for a spectrum of subclinical disease. The relation between sonographically measured colonic wall thickness and pancreatic enzyme preparation and dose was studied in 86 children with cystic fibrosis (CF). Colonic wall thickness of a control group was also measured. The average thickness in all colonic regions was higher in the CF group (overall average range 0.7-2.5 mm v 0.6-1.4 mm in the control group). There was no significant relation between colonic wall thickness and age, sex, total dose of lipase, or copolymer. Apart from one patient with an early colonic stricture, none of those exposed to high doses of lipase, or the methacrylic acid copolymer Eudragit L30 D55, showed evidence of subclinical damage to the colon. The reproducibility of the sonographic measurements was poor. 相似文献
80.
MT Britto JM Garrett MA Dugliss CW Daeschner CA Johnson MW Leigh JM Majure WH Schultz TR Konrad 《Pediatrics》1998,101(2):250-256
OBJECTIVE: To determine the prevalence and age of onset of common risky behaviors such as smoking and sexual activity in teens with cystic fibrosis and those with sickle cell disease and to compare their behaviors with those of adolescents in the general population. DESIGN: Survey. SETTING: All five major pediatric tertiary care centers in North Carolina (study participants with sickle cell disease or cystic fibrosis) and North Carolina public schools (comparison population). PARTICIPANTS: Three hundred twenty-one adolescents with cystic fibrosis or sickle cell disease aged 12 to 19 years (mean age, 15.6 years; 49% female). Demographically matched comparison teens for each group were selected from 2760 in-school adolescents (mean age, 16.0 years; 51% female). MAIN OUTCOMES MEASURES: Prevalence of tobacco and marijuana use, alcohol use, sexual intercourse, sexually transmitted diseases, seat belt use, weapon carrying, and age of onset of these behaviors. RESULTS: Chronically ill teens reported significantly less lifetime and current use of tobacco, marijuana, and alcohol; less sexual intercourse; less weapon carrying, less drunk driving, and more seat belt use than their peers. Nonetheless, 21% of the teens with cystic fibrosis and 30% of those with sickle cell disease had smoked; sexual intercourse was reported by 28% and 51%, respectively. Age of onset of these behaviors was frequently older for the chronically ill teens. CONCLUSION: Teens with cystic fibrosis or sickle cell disease took more potentially damaging health risks than might be expected, although the prevalence was lower than reported by their peers. Future longitudinal studies should examine the relationships between chronic illness, physical and psychosocial maturation, and risky behavior. Screening for psychosocial issues, including risky behaviors, should be incorporated into the routine health care of chronically ill teens. 相似文献