Suppression of mouse skin tumor promotion and induction of apoptosis in HL-60 cells by Alpinia oxyphylla Miquel (Zingiberaceae)

There have been considerable efforts to search for naturally occurring substances for the intervention of carcinogenesis. Many components from dietary or medicinal plants have been identified that possess substantial chemopreventive properties. An example is curcumin (Curcuma longa Linn., Zingiberaceae), which has been shown to inhibit tumor promotion in experimental carcinogenesis. Alpinia oxyphylla Miquel, another plant of the ginger family used in oriental herbal medicine, contains diarylheptanoids whose structures are analogous to that of curcumin. In the present study, we have tested A.oxyphylla for its ability to suppress tumor promotion. Thus, topical application of the methanolic extract of dried fruits of A.oxyphylla significantly ameliorated 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin tumor promotion as well as ear edema in female ICR mice. In another study, treatment of HL-60 cells with the methanolic extract of A.oxyphylla significantly reduced the viability of the cells and also inhibited DNA synthesis. Microscopic examination of the treated cells showed characteristic morphology of apoptosis. Furthermore, cells treated with the extract of A.oxyphylla exhibited internucleosomal DNA fragmentation in time- and concentration-dependent manners. TPA- stimulated generation of superoxide anion in differentiated HL-60 cells was also blunted by A.oxyphylla. Taken together, these findings suggest that A.oxyphylla possesses potential chemopreventive and antitumorigenic activities.      

对陈香露白露片检验中有关问题的探讨

目的：探讨部颁标准陈香露片检验中存在的问题。方法：Ｅ和部颁标准和改进方法对陈香露片中的铋盐进行定性一定量、镁盐进行定性。结果：该片中的铋盐磺四鉴别反应。用稀硝酸代替盐酸做溶剂,呈正反应、镁盐氯化铵＝磷酸氢二钠鉴别反应,加氨试液后,析出的白色沉淀不足Ｍｇ（ＯＨ）２,而是Ｂｉ（ＯＨ）３,该深沉在氯化铵试液中不溶,干扰镁盐鉴别;铋盐含量测定项,用加抗坏血酸掩蔽剂的方法,排除了Ｆｅ＾３＋的干扰,使终点颜色