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71.
72.
Agreeing with Damasio’s statement defining the “process” of consciousness, we propose the self as created by mind-based knowledge and a combination of images of an organism’s intentional motor responses interacting with its environment. The lemniscal system, with plastic capabilities, manages gravity in voluntary movement. The spinal segment motor reflex represents the schema of gravity-managing neuronal activity, and it can become “nested” in cortical areas participating in consciousness-building, allowing consideration of the brain as a hyper-evolved nervous system segment harboring atavic spinal organization. Consciousness’ capability to change itself makes humans co-participants in their own mental and consciousness evolution. 相似文献
73.
Conrad P. Quinn Carol L. Sabourin Nancy A. Niemuth Han Li Vera A. Semenova Thomas L. Rudge Heather J. Mayfield Jarad Schiffer Robert S. Mittler Chris C. Ibegbu Jens Wrammert Rafi Ahmed April M. Brys Robert E. Hunt Denyse Levesque James E. Estep Roy E. Barnewall David M. Robinson Brian D. Plikaytis Nina Marano 《Clinical and Vaccine Immunology : CVI》2012,19(11):1730-1745
A 3-dose (0, 1, and 6 months) intramuscular (3-IM) priming series of a human dose (HuAVA) and dilutions of up to 1:10 of anthrax vaccine adsorbed (AVA) provided statistically significant levels of protection (60 to 100%) against inhalation anthrax for up to 4 years in rhesus macaques. Serum anti-protective antigen (anti-PA) IgG and lethal toxin neutralization activity (TNA) were detectable following a single injection of HuAVA or 1:5 AVA or following two injections of diluted vaccine (1:10, 1:20, or 1:40 AVA). Anti-PA and TNA were highly correlated (overall r2 = 0.89 for log10-transformed data). Peak responses were seen at 6.5 months. In general, with the exception of animals receiving 1:40 AVA, serum anti-PA and TNA responses remained significantly above control levels at 28.5 months (the last time point measured for 1:20 AVA), and through 50.5 months for the HuAVA and 1:5 and 1:10 AVA groups (P < 0.05). PA-specific gamma interferon (IFN-γ) and interleukin-4 (IL-4) CD4+ cell frequencies and T cell stimulation indices were sustained through 50.5 months (the last time point measured). PA-specific memory B cell frequencies were highly variable but, in general, were detectable in peripheral blood mononuclear cells (PBMC) by 2 months, were significantly above control levels by 7 months, and remained detectable in the HuAVA and 1:5 and 1:20 AVA groups through 42 months (the last time point measured). HuAVA and diluted AVA elicited a combined Th1/Th2 response and robust immunological priming, with sustained production of high-avidity PA-specific functional antibody, long-term immune cell competence, and immunological memory (30 months for 1:20 AVA and 52 months for 1:10 AVA). Vaccinated animals surviving inhalation anthrax developed high-magnitude anamnestic anti-PA IgG and TNA responses. 相似文献
74.
Ben Davidson Vera Maria Abeler Ellen Hellesylt Arild Holth Ie-Ming Shih Tone Skeie-Jensen Li Chen Yanqin Yang Tian-Li Wang 《Gynecologic oncology》2013,128(2):349-355
ObjectiveEndometrial stromal sarcoma (ESS) and leiomyosarcoma (LMS) are the two most common uterine sarcomas, but both are rare tumors. The aim of the present study was to compare the global gene expression patterns of ESS and LMS.MethodsGene expression profiles of 7 ESS and 13 LMS were analyzed using the HumanRef-8 BeadChip from Illumina. Differentially expressed candidate genes were validated using quantitative real-time PCR and immunohistochemistry.ResultsUnsupervised hierarchical clustering using all 54,675 genes in the array separated ESS from LMS samples. We identified 549 unique probes that were significantly differentially expressed in the two malignancies by greater than 2-fold with 1% FDR cutoff using one-way ANOVA with Benjamini–Hochberg correction, of which 336 and 213 were overexpressed in ESS and LMS, respectively. Genes overexpressed in ESS included SLC7A10, EFNB3, CCND2, ECEL1, ITM2A, NPW, PLAG1 and GCGR. Genes overexpressed in LMS included CDKN2A, FABP3, TAGLN, JPH2, GEM, NAV2 and RAB23. The top 100 genes overexpressed in LMS included those coding for myosin light chain and caldesmon, but not the genes coding for desmin or actin. CD10 was not overexpressed in ESS. Results for selected genes were validated by quantitative real-time PCR and immunohistochemistry.ConclusionsWe present the first study in which gene expression profiling was shown to distinguish between ESS and LMS. The molecular signatures unique to each of these malignancies may aid in expanding the diagnostic battery for their differentiation, and may provide a molecular basis for prognostic studies and therapeutic target discovery. 相似文献
75.
Goldie Y.L. Lui Zaklina Kovacevic Vera Richardson Angelica M. Merlot Danuta S. Kalinowski Des R. Richardson 《Oncotarget》2015,6(22):18748-18779
Newer and more potent therapies are urgently needed to effectively treat advanced cancers that have developed resistance and metastasized. One such strategy is to target cancer cell iron metabolism, which is altered compared to normal cells and may facilitate their rapid proliferation. This is supported by studies reporting the anti-neoplastic activities of the clinically available iron chelators, desferrioxamine and deferasirox. More recently, ligands of the di-2-pyridylketone thiosemicarbazone (DpT) class have demonstrated potent and selective anti-proliferative activity across multiple cancer-types in vivo, fueling studies aimed at dissecting their molecular mechanisms of action. In the past five years alone, significant advances have been made in understanding how chelators not only modulate cellular iron metabolism, but also multiple signaling pathways implicated in tumor progression and metastasis. Herein, we discuss recent research on the targeting of iron in cancer cells, with a focus on the novel and potent DpT ligands. Several key studies have revealed that iron chelation can target the AKT, ERK, JNK, p38, STAT3, TGF-β, Wnt and autophagic pathways to subsequently inhibit cellular proliferation, the epithelial-mesenchymal transition (EMT) and metastasis. These developments emphasize that these novel therapies could be utilized clinically to effectively target cancer. 相似文献
76.
Right heart function in impaired left ventricular diastolic function: 2D speckle tracking echocardiography–based and Doppler tissue imaging–based analysis of right atrial and ventricular function 下载免费PDF全文
Anna Brand MD Marny Bathe Sabine Oertelt‐Prigione MScPH Ute Seeland MD Mirjam Rücke MSc Vera Regitz‐Zagrosek MD Karl Stangl MD Fabian Knebel MD Verena Stangl MD Henryk Dreger MD 《Echocardiography (Mount Kisco, N.Y.)》2018,35(1):47-55
Aim
The aim of our study was to describe right atrial (RA) and right ventricular (RV) function, assessed by Doppler tissue imaging and 2D speckle tracking echocardiography (2DSTE), in women with signs of early impaired left ventricular diastolic function (DD).Methods and Results
In a cross‐sectional trial, standard parameters of diastolic and right heart function were investigated in 438 women of the Berlin Female Risk Evaluation (BEFRI) study. In a subset of women, average peak systolic RA strain (RAS), as well as the average peak systolic RV strain of the free wall (RVS free wall) and of all RV segments (average RV strain; RVS Avg), was analyzed using 2DSTE. Compared to women with normal diastolic function (DD0), RAS, RVS free wall and RVS Avg were significantly reduced in DD (43.1% ± 11.9%, ?26.7% ± 5.6%, and ?23.3% ± 3.5% in DD0; vs 35.1% ± 10.4%, ?23.9% ± 5.5%, and ?20.6% ± 3.8% in DD; P < .01). Peak RV myocardial velocity (RV‐IVV) and acceleration during isovolumetric contraction (RV‐IVA) were markedly higher in DD (15.0 ± 3.9 cm/s and 3.1 ± 1.0 m/s² in DD vs 11.9 ± 3.2 cm/s and 2.8 ± 0.8 m/s² in DD0; P < .05). RAS and RV‐IVV were significantly associated with DD after adjustment to age, BMI, and left atrial strain in multivariate regression analysis.Conclusion
Systolic right heart function is significantly altered in DD. DTI as well as 2DSTE constitute sensitive echocardiographic tools that enable the diagnosis of impaired right heart mechanics in early‐staged DD. 相似文献77.
Thiago Martins Lara Ludhmila Abrahao Hajjar Juliano Pinheiro de Almeida Julia Tizue Fukushima Carmem Silvia Valente Barbas Adriano Rogerio Baldacin Rodrigues Emilia Nozawa Maria Ignes Zanetti Feltrim Elisangela Almeida Vera Coimbra Eduardo Osawa Rafael de Moraes Ianotti Alcino Costa Leme Fabio Biscegli Jatene Jose Otavio Costa Auler-Jr. Filomena Regina Barbosa Gomes Galas 《Clinics (S?o Paulo, Brazil)》2013,68(1):33-38
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80.
Luciana Furlaneto-Maia Kátia Real Rocha Vera Lúcia Dias Siqueira Márcia Cristina Furlaneto 《Revista do Instituto de Medicina Tropical de S?o Paulo》2014,56(2):97-103
Enterococci are increasingly responsible for nosocomial infections
worldwide. This study was undertaken to compare the identification and
susceptibility profile using an automated MicrosScan system, PCR-based assay and
disk diffusion assay of Enterococcus spp. We evaluated 30
clinical isolates of Enterococcus spp. Isolates were identified
by MicrosScan system and PCR-based assay. The detection of antibiotic resistance
genes (vancomycin, gentamicin, tetracycline and erythromycin) was also
determined by PCR. Antimicrobial susceptibilities to vancomycin (30 µg),
gentamicin (120 µg), tetracycline (30 µg) and erythromycin (15 µg) were tested
by the automated system and disk diffusion method, and were interpreted
according to the criteria recommended in CLSI guidelines. Concerning
Enterococcus identification the general agreement between
data obtained by the PCR method and by the automatic system was 90.0% (27/30).
For all isolates of E. faecium and E. faecalis
we observed 100% agreement. Resistance frequencies were higher in E.
faecium than E. faecalis. The resistance rates
obtained were higher for erythromycin (86.7%), vancomycin (80.0%), tetracycline
(43.35) and gentamicin (33.3%). The correlation between disk diffusion and
automation revealed an agreement for the majority of the antibiotics with
category agreement rates of > 80%. The PCR-based assay, the
van(A) gene was detected in 100% of vancomycin resistant
enterococci. This assay is simple to conduct and reliable in the identification
of clinically relevant enterococci. The data obtained reinforced the need for an
improvement of the automated system to identify some enterococci. 相似文献