Pericardiocentesis and systemic cytotoxic chemotherapy in the management of cardiac tamponade secondary to disseminated breast carcinoma.

Three patients with cardiac tamponade secondary to disseminated breast carcinoma were treated by pericardiocentesis followed by systemic cytotoxic chemotherapy. This approach controlled pericardial effusion in all the patients and extended their survival.     

Development and use of influenza vaccines

The development and use of effective influenza vaccines remains an important aspect of attempts to control the disease. Most influenza vaccination programmes use inactivated vaccines, but attenuated live vaccines are used in several countries. Studies comparing the relative efficacies of live and inactivated vaccines are needed.     

The Kmod blood group phenotype in a healthy individual

This report describes a healthy blood donor whose red cells have weakened expression of Kell blood group antigens. Kell antigen activity could not be detected by flow cytometric analysis and was demonstrable only by sensitive serologic techniques. As with normal-strength Kell antigens, reactivity could be abolished by treatment with 2-aminoethylisothiouronium bromide (AET). The donor's red cells have Kx antigen activity. Other commonly tested blood group antigens (MNSs, Rh, P1, Lewis, Duffy, and Kidd systems) appear normal. Clinical and serologic examination showed that this case is different from previously described examples of modified Kell expression. The propositus's phenotype has remained unchanged for 19 months, which suggests that it is not a transient condition. However, family studies provide no evidence that it is inherited. A 93-kD protein, which reacted weakly by Western blot with rabbit antibody to Kell protein, was isolated from the propositus's red cells by immunoprecipitation. This finding was not reproduced in subsequent studies, which suggests that the quantity of Kell protein recovered was at the threshold level detectable by the technique used. The red cell phenotype is categorized as Kmod, of which this is the first example reported in a healthy individual.     

黄芪复方制剂对氢化可的松所致大鼠与小鼠骨质疏松的影响

用氢化可的松造成实验性骨质疏松，观察黄芪复方制剂防止骨质丢失的作用。结果表明，模型组与给药组动物骨钙、骨磷和羟脯氨酸含量有显著差异，ＰＡＭ可防止氢化可的松引起的鼠骨矿物质及有机物质的丢失。     

The VLA-2 (alpha 2 beta 1) I domain functions as a ligand-specific recognition sequence for endothelial cell attachment and spreading: molecular and functional characterization

The integrin VLA-2 (alpha 2 beta 1), generally considered to represent the specific collagen receptor on human endothelial cells, contains an alpha 2-subunit inserted I domain with structural similarity to the type A domains found within the recently described superfamily of receptor-ligand recognition proteins. This region of the cDNA has now been isolated and used for molecular and functional characterization of this heterodimeric receptor complex. Comparative sequence analysis with the porcine homologue revealed 93% amino acid sequence identity, suggestive of a developmentally conserved function. To complete structure/function studies, this region of the human cDNA was expressed as a chimeric protein in Escherichia coli, and a rabbit polyclonal antibody (anti-I domain) was used to study determinants of endothelial cell attachment and spreading in vitro. Quantifiable and visual disruption of endothelial cell attachment to gelatin, type I collagen, and laminin was evident using the specific anti-I domain antibody, with minimal inhibitory effects demonstrable using fibronectin or fibrinogen matrices. Therefore, these data would suggest that the alpha 2 beta 1 I domain confers ligand-binding specificity for both known alpha 2 beta 1 substrates (laminin and collagen), and that this region subserves a regulatory function in the molecular processes controlling endothelial cell attachment and spreading in vitro.     

蕊木宁在大鼠体内分布与排泄的动态研究

目的 :研究云南蕊木中有效成分蕊木宁在大鼠体内的组织分布与排泄。方法 :将蕊木宁用放射性元素 3H标记 ,分别以静注和口服方式给予受试大鼠 ,收集血液、脏器、尿液、胆汁等 ,用液体闪烁记数仪测量其放射活性。结果 :血中的放射活性呈二相衰减 ,t1/ 2α为 12 min,t1/ 2β 2 8.1h。口服给药 1h后 ,肝脏中的放射物浓度为 2 77.5± 17.8ng/ g,肾脏为 182 .4± 12 .0 ng/ g;并且 ,48h内 ,大部分放射活性 (6 6 % )由尿液排泄 ,小部分 (14% )由胆汁排泄。结论 :肝脏可能是蕊木宁的主要靶器官 ,且蕊木宁自身产生肝肠循环的可能性较小