全文获取类型
收费全文 | 765篇 |
免费 | 49篇 |
国内免费 | 43篇 |
专业分类
耳鼻咽喉 | 11篇 |
儿科学 | 9篇 |
妇产科学 | 4篇 |
基础医学 | 95篇 |
口腔科学 | 8篇 |
临床医学 | 79篇 |
内科学 | 151篇 |
神经病学 | 62篇 |
特种医学 | 96篇 |
外科学 | 50篇 |
综合类 | 14篇 |
预防医学 | 88篇 |
眼科学 | 8篇 |
药学 | 87篇 |
中国医学 | 4篇 |
肿瘤学 | 91篇 |
出版年
2023年 | 7篇 |
2022年 | 4篇 |
2021年 | 18篇 |
2020年 | 6篇 |
2019年 | 8篇 |
2018年 | 11篇 |
2017年 | 10篇 |
2016年 | 13篇 |
2015年 | 20篇 |
2014年 | 23篇 |
2013年 | 38篇 |
2012年 | 30篇 |
2011年 | 21篇 |
2010年 | 32篇 |
2009年 | 24篇 |
2008年 | 31篇 |
2007年 | 47篇 |
2006年 | 31篇 |
2005年 | 42篇 |
2004年 | 31篇 |
2003年 | 19篇 |
2002年 | 24篇 |
2001年 | 18篇 |
2000年 | 30篇 |
1999年 | 19篇 |
1998年 | 20篇 |
1997年 | 10篇 |
1996年 | 16篇 |
1995年 | 8篇 |
1994年 | 9篇 |
1993年 | 17篇 |
1992年 | 20篇 |
1991年 | 17篇 |
1990年 | 17篇 |
1989年 | 19篇 |
1988年 | 19篇 |
1987年 | 12篇 |
1986年 | 14篇 |
1985年 | 8篇 |
1984年 | 4篇 |
1983年 | 8篇 |
1982年 | 13篇 |
1981年 | 7篇 |
1980年 | 12篇 |
1978年 | 6篇 |
1977年 | 9篇 |
1976年 | 9篇 |
1975年 | 4篇 |
1970年 | 5篇 |
1910年 | 3篇 |
排序方式: 共有857条查询结果,搜索用时 662 毫秒
81.
82.
83.
84.
85.
目的:从细胞水平,观察扶元起萎,养荣生肌的中药制剂肌萎灵注射液对原代培养鼠胚脊髓运动神经元的保护作用。方法:实验于2004-03/2005-03在解放军第三军医大学完成。实验分组:清洁级SD雌性孕鼠,孕期10~14d。应用密度梯度离心法分离鼠胚脊髓运动神经元进行原代培养,运动神经元培养72h后,按培养板及孔分为4.5μg/L肌萎灵组(肌萎灵注射液,含生药0.9g/mL)、9μg/L肌萎灵组、45μg/L肌萎灵组、力如太组(力如太R利鲁唑片,应用时经0.9%NaCl-0.01NHCl溶解)和对照组。实验处理:各组分别加入用新鲜培养基稀释为0.5%(终浓度4.5μg/L)、1%(终浓度9μg/L)、5%(终浓度45μg/L)的肌萎灵注射液,力如太组加入利鲁唑(终浓度10μmo/L),对照组加入等量新鲜培养基。实验评估:①共同培养3d用四甲基偶氮唑盐法观察其对细胞活力的影响,以吸光度表示。②采用NF-200免疫组织化学染色并进行图像分析,测定神经突起主干长度。结果:①培养的运动神经元活力状态比较:4.5,9,45μg/L肌萎灵组培养运动神经元活力显著增强,与对照组相比,差异有显著性意义(0.317±0.054,0.396±0.087,0.329±0.097,0.230±0.130,P<0.05)。力如太组细胞生长与对照组相比无显著差异(0.266±0.141,0.230±0.130,P>0.05)。②脊髓运动神经元突起生长情况结果:4.5μg/L肌萎灵组和9μg/L肌萎灵组可促进其生长,与对照组相比,差异有显著性意义[(315.96±32.32),(373.46±80.24),(159.71±48.95)μm,P<0.05]。结论:肌萎灵注射液可增强运动神经元活力,促进脊髓运动神经元突起的生长。 相似文献
86.
Bernstein DI Cartier A Côté J Malo JL Boulet LP Wanner M Milot J L'Archevéque J Trudeau C Lummus Z 《American journal of respiratory and critical care medicine》2002,166(4):445-450
We previously reported that diisocyanate-human serum albumin (DIISO-HSA) stimulated production of monocyte chemoattractant protein-1 (MCP-1) by peripheral blood mononuclear cells is significantly associated with a clinical diagnosis of diisocyanate asthma (DA). Others have reported that antibodies for DIISO-HSA are specific but insensitive markers of DA. This study was performed to evaluate test characteristics of the in vitro MCP-1 assay compared with DIISO-HSA-specific immunoglobulin (Ig) G and IgE in identifying workers with DA. MCP-1 was quantitated in peripheral blood mononuclear cell supernatants 48 hours after incubation with DIISO-HSA antigens. Assay results were compared with outcomes of specific inhalation challenge (SIC) testing. Nineteen of 54 (35%) workers assayed for antibodies and MCP-1 stimulation had SIC-confirmed DA. Mean MCP-1 produced by SIC-positive workers was greater than SIC-negative workers (p < or = 0.001). Diagnostic sensitivity, specificity, and test efficiency for specific IgG were 47%, 74%, and 65%, respectively, and for specific IgE were 21%, 89%, and 65%, respectively. Sensitivity, specificity, and test efficiency of the MCP-1 test were 79%, 91%, and 87%, respectively. This study indicates that the MCP-1 stimulation assay has greater sensitivity and specificity than the specific antibody assays in correctly identifying DA. 相似文献
87.
88.
S. Djalalov J. Beca E. Amir M. Krahn M.E. Trudeau J.S. Hoch 《Current oncology (Toronto, Ont.)》2015,22(2):84-96
Background
Aromatase inhibitor (ai) therapy has been subjected to numerous cost-effectiveness analyses. However, with most ais having reached the end of patent protection and with maturation of the clinical trials data, a re-analysis of ai cost-effectiveness and a consideration of ai use as part of sequential therapy is desirable. Our objective was to assess the cost-effectiveness of the 5-year upfront and sequential tamoxifen (tam) and ai hormonal strategies currently used for treating patients with estrogen receptor (er)–positive early breast cancer.Methods
The cost-effectiveness analysis used a Markov model that took a Canadian health system perspective with a lifetime time horizon. The base case involved 65-year-old women with er-positive early breast cancer. Probabilistic sensitivity analyses were used to incorporate parameter uncertainties. An expected-value-of-perfect-information test was performed to identify future research directions. Outcomes were quality-adjusted life-years (qalys) and costs.Results
The sequential tam–ai strategy was less costly than the other strategies, but less effective than upfront ai and more effective than upfront tam. Upfront ai was more effective and less costly than upfront tam because of less breast cancer recurrence and differences in adverse events. In an exploratory analysis that included a sequential ai–tam strategy, ai–tam dominated based on small numerical differences unlikely to be clinically significant; that strategy was thus not used in the base-case analysis.Conclusions
In postmenopausal women with er-positive early breast cancer, strategies using ais appear to provide more benefit than strategies using tam alone. Among the ai-containing strategies, sequential strategies using tam and an ai appear to provide benefits similar to those provided by upfront ai, but at a lower cost. 相似文献89.
90.