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61.
Tissue regeneration using macrophage migration inhibitory factor-impregnated gelatin microbeads in cutaneous wounds 下载免费PDF全文
Zhao Y Shimizu T Nishihira J Koyama Y Kushibiki T Honda A Watanabe H Abe R Tabata Y Shimizu H 《The American journal of pathology》2005,167(6):1519-1529
Migration inhibitory factor (MIF) responds to tissue damage and regulates inflammatory and immunological processes. To elucidate the function of MIF in cutaneous wound healing, we analyzed MIF knockout (KO) mice. After the excision of wounds from the dorsal skin of MIF KO and wild-type (WT) mice, healing was significantly delayed in MIF KO mice compared to WT mice. Lipopolysaccharide treatment significantly increased [(3)H]thymidine uptake in WT mouse fibroblasts compared to MIF KO mouse fibroblasts. Furthermore, there was a significant reduction in fibroblast and keratinocyte migration observed in MIF KO mice after 1-oleoyl-2-lysophosphatidic acid treatment. We subsequently examined whether MIF-impregnated gelatin slow-release microbeads could accelerate skin wound healing. Injection of more than 1.5 microg/500 microl of MIF-impregnated gelatin microbeads around a wound edge accelerated wound healing compared to a single MIF injection without the use of microbeads. MIF-impregnated gelatin microbeads also accelerated skin wound healing in C57BL/6 mice and diabetic db/db mice. Furthermore, incorporating MIF-impregnated gelatin microbeads into an artificial dermis implanted into MIF KO mice accelerated procollagen production and capillary formation. These findings suggest that MIF is crucial in accelerating cutaneous wound healing and that MIF-impregnated gelatin microbeads represent a promising treatment to facilitate skin wound healing. 相似文献
62.
Probiotics have been suggested to be effective for functional dyspepsia, but their effect on gastric motility is not clear. We evaluated the effect of Lactobacillus gasseri OLL2716 (LG21 strain) on mild to moderate delayed gastric emptying by a double-blind, parallel-group, placebo-controlled, randomized trial. Participants (n = 28) were randomly assigned to ingest LG21 strain-containing yogurt (LG21 strain group) or LG21 strain-free yogurt (placebo group) for 12 weeks. The 13C gastric emptying breath test was performed to measure the gastric emptying rate over time following ingestion of a liquid meal, and the time to reach the peak (Tmax) was used as an indicator of gastric emptying. We also measured the salivary amylase concentration, an indicator of autonomic dysfunction under stress. The per-protocol population (n = 27, male n = 4, female n = 23) was evaluated for efficacy. When a ≥30% reduction in the difference between participant’s Tmax and the Japanese mean Tmax was defined as an improvement, the odds ratio of improvement in delayed gastric emptying compared to placebo after 12 weeks was 4.1 (95% confidence interval, 0.8 to 20.2). Moreover, salivary amylase concentrations were significantly lower than in the placebo group, indicating an improvement in autonomic function. The present data were not enough to support the beneficial effects of the LG21 strain on delayed gastric emptying. However, if we define the odds ratio in further study investigated with a larger number of participants, LG21 strain might be expected to have some impact on delayed gastric emptying. 相似文献
63.
64.
Toshihiro Kawamoto Koji Matsuno Keiichi Arashidani Masahiro Yoshikawa Fujio Kayama Yasushi Kodama 《Archives of environmental contamination and toxicology》1993,25(4):534-538
The personal exposure to NO2 generated from various heaters and cooking stoves were studied, using 85 university students. The students attached NO2 filter badges to their chests or collars and wrote down the period of time for heating and cooking for 1 week. Types of heaters and smoking habits were described through a questionnaire. The urinary hydroxyproline/creatinine ratio (HOP/C) was examined as a biomarker for health effects. The outdoor NO2 concentration during the study period was 13.5–13.7 g/m3. Smoking and the usage of electric heaters did not affect the exposure to NO2. Exposure increased according to the length of time kerosene heaters or oil fan heaters were used. The NO2 concentration during the heating by a kerosene heater and an oil fan heater was calculated to be 219 and 474 g/m3, respectively. The correlation between the period of cooking and personal exposure was also observed. The NO2 levels during cooking were calculated to be 290 g/m3. Using these calculated values of NO2 concentration, it is possible to presume the personal exposure levels from the length of time heaters and cooking stoves are used even if the subjects do not attach the filter badges. Neither smoking nor exposure to NO2 were associated with the increase of urinary HOP/C. 相似文献
65.
Great horovement haS been made in the cryopreservation Of inalnlnalian elnbmp since wnttingham['] reported his Pioneering wold about successful cryOPreservationof mouse embryo. It results in the availability of cryOPreserVation of embryos of all stages[']. HOwever, cryopreserved embryos can not avoid the damages by ice crystal,which is a major cause of cen death in the freezing andthawing ProceduresL',']. SO it is necesseq to seareh for anew and more efficient method for embryO cryOPrese… 相似文献
66.
Ohta T Fukuda M Arima K Kawamoto H Hashizume R Arimura T Yamaguchi S 《Breast cancer (Tokyo, Japan)》1997,4(1):17-24
Cyclins and cyclin-dependent kinases may reflect the status of cell proliferation in cancer tissues. The authors sought to
determine whether cdc2 and cyclin D1 are expressed in breast cancer and are useful as prognostic factors. Accumulation of
cdc2 and cyclin D1 proteins was examined in 88 cases of breast cancer using immunoblotting techniques and correlations with
clinicopathological factors and prognoses were investigated. Cdc2 and cyclin D1 proteins were observed in 27.3% and 75.0%
of breast cancers studied, respectively. The incidence of lymph node metastasis was significantly high in cdc2/cyclin D1-double
positive group and low in double negative group. On the other hand, the incidence of estrogen receptor (ER) negative cases
was significantly higher in the cdc2-positive/cyclin D1-negative group. Relapse-free survival times of cdc2-positive cases
were significantly shorter than those of cdc2-negative cases. The relapse-free survival times of cyclin D1-positive cases
also tended to be poorer than those of cyclin D1-negative cases. Multivariate analyses revealed cdc2 as the second most significant
of the prognostic variables, following lymph node status. The three-year relapse-free survival rate of cdc2/cyclin D1-double
positive cases was 58.9%, whereas that of cdc2/cyclin D1-double negative cases was 100%. Cdc2 and cyclin D1 represent the
status of cell proliferation in breast cancer, and may be useful in breast cancer assessment. 相似文献
67.
The gravity model, a method for analyzing transportation distribution in transportation engineering, was used to explain patient
trips between ten health service regions in a Japanese prefecture. The OD (Origin-Destination) tables were constructed with
zoning by regions, distinguishing between out- and inpatients. The observed trips were determined from the data of a survey
conducted in 1992 that reported the locations of patient residences and chosen medical facilities.
The base values used in the model calculations were the population size of each region and the road distance between the centers
of regions. Problems of intrazonal trip were avoided by setting a mean intrazonal movement distance.
This model was calibrated by the linear regression method with simultaneous validation by the index of correlation coefficients.
The model was found to accurately simulate the effect of distance on the choice of medical facilities and the differences
between the characteristics of in- and out-patients. The population value in use showed the relation not only with demand
but also the supply of clinical services. It was suggested that the model presented here was useful in the allocation of medical
resources and would help explain the relationship between suppliers and consumers of medical services. 相似文献
68.
69.
Finger bougie method compared with pyloroplasty in the gastric replacement of the esophagus 总被引:2,自引:0,他引:2
Yamashita Y Hirai T Mukaida H Yoshimoto A Kuwahara M Inoue H Toge T 《Surgery today》1999,29(2):107-110
To elucidate the necessity of pyloroplasty for the gastric tube through the posterior mediastinum in esophageal surgery, gastric
emptying and duodenogastric reflux (DGR) were evaluated in 16 cases undergoing an anterior pylorectomy (group P) and in 16
cases treated by the finger bougie method (group F). First, the obstruction and reflux symptoms were examined based on a patient
questionnaire using a brief scoring system. The median value of the symptom score showed the patients in P to have more symptoms
than those in F; however, the difference was not significant (8.0 vs 6.0). Secondly, the swallowed Tc O4
− (85 MBq) was counted using a gamma camera at three sites on the sternal bone in the upright position based on a gastric transit
scintigram. Both the descending time of the RI peak and the clearance rates were similar between the two groups. Thirdly,
intragastric 24-h pH monitoring was carried out. Antimony pH sensors were anchored 5 and 15 cm below the esophagogastrostomy.
We could not find any difference between the two groups in both the % time pH>4 and %time pH>7. These findings thus revealed
no big difference between groups P and F. The finger bougie method to drain the vagotomized posterior mediastinal stomach
was found to achieve results similar to conventional pyloroplasty, while it was also simpler and safer. 相似文献
70.
We examined the effects of a new stable prostacyclin analogue, OP-2507, on myocardial function and metabolism in the ischemic working rat heart preparation. The hearts were perfused with Krebs-Henseleit bicarbonate (KHB) buffer, and whole heart ischemia was induced by one-way aortic valve for 15min follows by reperfusion for 30min. In the treated hearts, OP-2507, 20ng·ml–1, was administered to KHB buffer from the beginning to the end of experiment. During ischemia, coronary flow in the OP-2507 group increased significantly more than that in the control group. The mechanical performance of both groups was impaired after ischemia. However, the recovery of coronary flow, cardiac output, peak systolic pressure and LV dP/dTmax was significantly higher in the treated group than in the control group. The incidence of ventricular fibrillation during reperfusion was 100% and 25% in the control and the OP-2507 groups, respectively. Myocardial ATP content was significantly higher in the treated hearts than that in the control hearts. These results indicate that this stable prostacyclin analogue is beneficial in myocardial ischemia, even without its well known action of preventing platelet aggregation.(Oguchi T, Kashimoto S, Nakamura T, et al.: Effects of prostacyclin analogue, OP-2507, on function and metabolism in the ischemic working rat heart. J Anesth 6: 446–454, 1992) 相似文献