全文获取类型
收费全文 | 2768篇 |
免费 | 148篇 |
国内免费 | 14篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 138篇 |
妇产科学 | 55篇 |
基础医学 | 302篇 |
口腔科学 | 37篇 |
临床医学 | 149篇 |
内科学 | 720篇 |
皮肤病学 | 24篇 |
神经病学 | 112篇 |
特种医学 | 183篇 |
外科学 | 606篇 |
综合类 | 14篇 |
预防医学 | 61篇 |
眼科学 | 116篇 |
药学 | 156篇 |
中国医学 | 3篇 |
肿瘤学 | 248篇 |
出版年
2023年 | 23篇 |
2022年 | 66篇 |
2021年 | 97篇 |
2020年 | 38篇 |
2019年 | 70篇 |
2018年 | 80篇 |
2017年 | 65篇 |
2016年 | 67篇 |
2015年 | 98篇 |
2014年 | 121篇 |
2013年 | 128篇 |
2012年 | 200篇 |
2011年 | 238篇 |
2010年 | 120篇 |
2009年 | 115篇 |
2008年 | 149篇 |
2007年 | 216篇 |
2006年 | 165篇 |
2005年 | 167篇 |
2004年 | 168篇 |
2003年 | 138篇 |
2002年 | 129篇 |
2001年 | 14篇 |
2000年 | 18篇 |
1999年 | 24篇 |
1998年 | 27篇 |
1997年 | 12篇 |
1996年 | 17篇 |
1995年 | 21篇 |
1994年 | 16篇 |
1993年 | 13篇 |
1992年 | 11篇 |
1991年 | 9篇 |
1990年 | 6篇 |
1989年 | 10篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1986年 | 6篇 |
1985年 | 7篇 |
1984年 | 5篇 |
1983年 | 3篇 |
1982年 | 5篇 |
1981年 | 4篇 |
1979年 | 3篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1974年 | 2篇 |
1971年 | 4篇 |
1970年 | 4篇 |
1967年 | 5篇 |
排序方式: 共有2930条查询结果,搜索用时 0 毫秒
91.
92.
Thaís Caroline Dallabona Dombroski Jose Eduardo Peixoto-Santos Karina Maciel Munira Muhammad Abdel Baqui Tonicarlo Rodrigues Velasco Americo Ceiki Sakamoto João Alberto Assirati Carlos Gilberto Carlotti Hélio Rubens Machado Gleice Kelly de Sousa Kenji Hanamura João Pereira Leite Jaderson Costa da Costa André Luiz Palmini Eliseu Paglioli Luciano Neder Roberto Spreafico Tomoaki Shirao Rita Garbelli Antonio Roberto Martins 《Epilepsia》2020,61(8):1581-1594
93.
Akira Igarashi Yasuhiro Ebihara Tomoaki Kumagai Hiroyuki Hirai Kinya Nagata Kohichiro Tsuji 《Allergology international》2018,67(2):234-242
Background
Several methods have been developed to detect allergen-specific IgE in sera. The passive IgE sensitization assay using human IgE receptor-expressing rat cell line RBL-2H3 is a powerful tool to detect biologically active allergen-specific IgE in serum samples. However, one disadvantage is that RBL-2H3 cells are vulnerable to high concentrations of human sera. Only a few human cultured cell lines are easily applicable to the passive IgE sensitization assay. However, the use of human induced pluripotent stem cells (iPSCs) to generate human mast cells (MCs) has not yet been reported.Methods
The nuclear factor-kappa B (NF-κB)-responsive luciferase reporter gene was stably introduced into a human iPSC line 201B7, and the transfectants were induced to differentiate into MCs (iPSC-MCs). The iPSC-MCs were sensitized overnight with sera from subjects who were allergic to cedar pollen, ragweed pollen, mites, or house dust, and then stimulated with an extract of corresponding allergens. Activation of iPSC-MCs was evaluated by β-hexosaminidase release, histamine release, or luciferase intensity.Results
iPSCs-MCs stably expressed high-affinity IgE receptor and functionally responded to various allergens when sensitized with human sera from relevant allergic subjects. This passive IgE sensitization system, which we termed the induced mast cell activation test (iMAT), worked well even with undiluted human sera.Conclusions
iMAT may serve as a novel determining system for IgE/allergens in the clinical and research settings. 相似文献94.
Sentinel lymph node mapping for gastric cancer using a dual procedure with dye- and gamma probe-guided techniques 总被引:8,自引:0,他引:8
Hayashi H Ochiai T Mori M Karube T Suzuki T Gunji Y Hori S Akutsu N Matsubara H Shimada H 《Journal of the American College of Surgeons》2003,196(1):68-74
BACKGROUND: Increasing evidence supports the sentinel lymph node (SN) concept for melanoma and breast cancers. SN biopsy may replace routine lymph node dissection in the treatment of these cancers. But there are little data evaluating this concept in patients with gastric cancer. The objective of this study was to test the feasibility of SN mapping in gastric cancers by using the dual-mapping procedure with dye and radioactive colloid. STUDY DESIGN: Thirty-one consecutive patients preoperatively diagnosed as T1-2 and N0 underwent SN biopsy using the dual-mapping procedure. Distributions of SNs identified by the dye-guided technique (blue nodes; BNs) were compared with those identified by the gamma probe guided technique (hot nodes; HNs). RESULTS: Among the 31 patients, 7 were found to have lymph node metastases. All positive nodes were detected by SN biopsy using the dual method. So, an accuracy rate of 100% was achieved in predicting the status of regional lymph nodes. Both BNs and HNs were identified in 28 of 31 patients (90%), but significant discrepancy of distribution was noted between BNs and HNs. Among the 28 patients with identified BNs, there was one metastasis in a non-BN. So the accuracy rate was 96% for the dye-guided technique. In contrast, among the 28 patients with identified HNs, 2 patients had metastasis in non-HNs, making the accuracy rate 93% for the gamma probe-guided technique. CONCLUSIONS: SN mapping is feasible in gastric cancer, but the dye-guided and gamma probe-guided techniques are complementary. So we recommend the dual-mapping procedure. 相似文献
95.
Incidence of urethral stricture after bipolar transurethral resection of the prostate using TURis: results from a randomised trial 下载免费PDF全文
96.
Midterm results of stent-graft repair of acute and chronic aortic dissection with descending tear: the complication-specific approach 总被引:12,自引:0,他引:12
Kato N Shimono T Hirano T Suzuki T Ishida M Sakuma H Yada I Takeda K 《The Journal of thoracic and cardiovascular surgery》2002,124(2):306-312
BACKGROUND: Endovascular stent-graft placement for the treatment of patients with aortic dissection is emerging as an attractive alternative to conventional cardiac operations. However, there has been no report of longer-term follow-up. The purpose of this study is to describe our midterm results with endovascular stent-graft repair for the treatment of patients with aortic dissections. METHODS: Thirty-eight patients with aortic dissections with descending tears were treated with endovascular stent-grafting. Ten patients had acute type A, 14 patients had acute type B, and 14 patients had chronic type B dissection. Stent grafts fabricated from expanded polytetrafluoroethylene-covered Z stents were placed to close entry tears in all patients through the delivery systems introduced from the femoral or the iliac arteries. RESULTS: Two patients with complicated acute type B dissection, who would have required surgical intervention, died within 30 days of the procedure, although no other patients died within the same period. There were no late deaths during the mean follow-up period of 27 months. Early and late complication rates were 33% and 36%, respectively, in patients with acute dissection, whereas rates were 4% and 0% (P <.05 vs patients with acute dissection) in patients with chronic dissection. CONCLUSIONS: Entry closure with endovascular stent-graft placement may be a safe and effective method for the treatment of patients with aortic dissection. It could be an alternative to conventional surgical intervention in selected patients with chronic dissection. However, strict patient selection and close follow-up seem mandatory in patients with acute dissection receiving Z stent-based stent-grafts. Stent-graft repair should be delayed for acute type B dissection without complications. 相似文献
97.
Koji Asai Manabu Watanabe Shinya Kusachi Hiroshi Matsukiyo Tomoaki Saito Hajime Kodama Toshiyuki Enomoto Yoichi Nakamura Yasushi Okamoto Yoshihisa Saida Raisuke Iijima Jiro Nagao 《Surgery today》2014,44(1):160-165
This report presents the case of a common hepatic artery (CHA) pseudoaneurysm secondary to postoperative pancreatic fistula (POPF) after pancreatoduodenectomy (PD), which was successfully treated using a coronary covered stent. A 70-year-old female underwent subtotal stomach-preserving PD for middle cholangiocarcinoma. POPF was identified on postoperative day (POD) 7, and the patient suddenly lost 500 ml of blood via the abdominal drain on POD 19. Urgent celiac arteriography revealed a CHA pseudoaneurysm. A coronary covered stent was placed to prevent rupture of the pseudoaneurysm and to maintain hepatic arterial flow, instead of performing transarterial embolization. No vascular adverse events were encountered during or after the procedure. Computed tomography and angiography showed a patent stent graft and good hepatic arterial flow 9 months after placement of the stent. Endovascular stent-graft placement not only treated the pseudoaneurysm, but also preserved the arterial blood flow. This report describes the placement of a covered stent graft for delayed hemorrhage after PD. 相似文献
98.
Both plasma lysophosphatidic acid and serum autotaxin levels are increased in chronic hepatitis C 总被引:1,自引:0,他引:1
Watanabe N Ikeda H Nakamura K Ohkawa R Kume Y Aoki J Hama K Okudaira S Tanaka M Tomiya T Yanase M Tejima K Nishikawa T Arai M Arai H Omata M Fujiwara K Yatomi Y 《Journal of clinical gastroenterology》2007,41(6):616-623
OBJECTIVES: Recent accumulating evidence indicates that lysophosphatidic acid (LPA) is a lipid mediator, abundantly present in blood, with a wide range of biologic actions including the regulation of proliferation and contraction in liver cells. Although it is speculated that LPA might play a role in pathophysiologic processes in vivo, not only its role but also even a possible alteration in its blood concentration under specific diseases is essentially unknown. Autotaxin (ATX), originally purified as an autocrine motility factor for melanoma cells, was revealed to be a key enzyme in LPA synthesis. We determined LPA and ATX levels in the blood of patients with liver disease. METHODS: ATX activity was measured by determining choline with the substrate of lysophosphatidylcholine, and the LPA level by an enzymatic cycling method in 41 patients with chronic hepatitis C. RESULTS: The serum ATX activity and plasma LPA level were significantly increased in patients, and were correlated positively with serum hyaluronic acid, and negatively with platelets, albumin, and prothrombin time. The plasma LPA level was strongly correlated with serum ATX activity. There were significant correlations between the histologic stage of fibrosis and both the serum ATX activity and plasma LPA level. CONCLUSIONS: The serum ATX activity and plasma LPA level are increased in chronic hepatitis C in association with liver fibrosis. Our study may provide the first evidence showing a significant increase of both ATX and LPA in the blood under a specific disease. 相似文献
99.
Hideyuki Ogawa Toshiyuki Ishikawa Kouhei Matsushita Katsumi Matsumoto Tomoaki Ishigami Teruyasu Sugano Kazuaki Uchino Satoshi Umemura Shinichi Sumita Kazuo Kimura Takeshi Nakagawa Makoto Shimizu Hideo Nishikawa Atsunobu Kasai Yukio Kioka 《Circulation journal》2008,72(5):700-704
BACKGROUND: Several preliminary studies have indicated that atrial pacing can prevent atrial tachyarrhythmias. The suggested mechanisms by which pacing may be effective include suppression of premature atrial beats. METHODS AND RESULTS: The Atrial Pacing Preference (APP; Guidant, St Paul, MN, USA) algorithm allows the pacemaker to maintain a pacing rate slightly higher than the sinus rate. The preventive effects of APP on paroxysmal atrial fibrillation (AF) were studied in 51 patients (70+/-11 years). Nine patients did not complete the protocol. The pacemaker was programmed in random order to APP off and APP on at 3 different settings (ie, 8, 16 and 32 cycles) for 4 weeks each, using a cross-over design. Percentage atrial pacing was lower in APP off than at the other settings. Premature beat counts were greater in APP off than at the other settings. There was a significant difference in mode switch episode counts between APP off and the most effective setting (3,818+/-15,356 vs 596+/-1,719; p<0.01). CONCLUSIONS: The APP algorithm is a promising method for preventing atrial tachyarrhythmia in patients with an implanted pacemaker and AF. Optimizing the setting of the APP algorithm is an important issue in the prevention of AF. 相似文献
100.
Kobayashi Y Ishihara T Wada M Kajihara S Araki J Mifuji R Itani T Kuroda M Urawa F Kaito M Adachi Y 《Journal of gastroenterology》2004,39(9):892-895
Black liver is a common finding in Dubin-Johnson syndrome (DJS), which is caused by the lack of multidrug resistance-associated protein 2 (MRP2). Impaired excretion of epinephrine metabolites is believed to be a cause of black liver in DJS. Recently, we experienced a patient with black liver whose serum bilirubin level was normal. Coarse brown granules were observed in the hepatocytes, and this finding closely resembled that observed in DJS. However, the granules were negative for Schmorl staining. The MRP2 gene did not show any mutation. Immunostaining study demonstrated MRP2 protein expression in the liver, and it was localized in the canalicular membranes of hepatocytes. This case illustrates for the first time that DJS is not the only cause of black liver. 相似文献