The prevalence of LRRK2 Gly2385Arg variant in Chinese Han population with Parkinson's disease.

We conducted a case-control study to determine the prevalence of the LRRK2 Gly2385Arg variant in patients with Parkinson's disease in Han population in mainland China. Heterozygous LRRK2 Gly2385Arg variant was identified in 14 of 235 patients with Parkinson's disease (5.69%), but not in 214 unrelated healthy controls. Multivariate analysis indicated the frequency of Gly2385Arg variant in the female patients with early age at onset is higher than their male counterparts. The founder haplotype analysis showed the variant carriers shared the same founder. Clinically, the LRRK2 Gly2385Arg carriers presented with classical Parkinson's disease symptoms. Our study indicates that the LRRK2 Gly2385Arg variant is a potential ethnic-specific genetic risk factor of Parkinson's disease within Chinese Han ethnicity.     

褪黑素在鸡脊柱侧凸模型中的影响

目的　通过鸡松果体蒂部切断的方法 ,研究褪黑素在松果体切除鸡脊柱侧凸动物模型中的作用。方法　 10只刚孵育出的WhiteLeghorn鸡作为对照组 ,未行任何处理 ,控制白天 12h光照 (强度 5 0 0Lux)、夜间 12h完全黑暗 (强度 0 - 5lux)条件下饲养 ;2 0只WhiteLeghorn鸡在鸡龄 3d时行松果体切除 ,控制光照条件同对照组 ;2 0只WhiteLeghorn鸡 3d时行松果体蒂部切断术 ,控制光照同对照组。 5周时留取所有鸡白天 (mid -day)和夜间 (mid -night)的血清 ,用RIA试剂盒测定血清中褪黑素含量。所有的鸡处死后 ,取骨骼标本 ,行脊柱前后位平片检查。结果　 5周时 ,对照组褪黑素含量呈现明显白天低 (5 7.2 5± 7.4 3)pg/ml,夜间高 (2 5 7.92± 2 6 .0 9)pg/ml的周期性变化。松果体切除组褪黑素含量 ,白天为 (6 0 .0 5± 5 .4 8)pg/ml,夜间为 (5 5 .0 9± 8.35 )pg/ml,其分泌维持低水平。松果体蒂部切断组褪黑素含量 ,白天为 (5 8.77± 8.4 4 )pg/ml,夜间为 (2 4 8.4 7± 2 7.2 1)pg/ml,仍呈白天低 ,夜间高的周期性变化。X线检查 :对照组 10只鸡无脊柱侧凸发生 ;松果体切除组 2 0只鸡中 9只鸡发生了脊柱侧凸 ,发生率为 4 5 % ;松果体蒂部切断组 2 0只鸡中有 11只发生了侧弯 ,发生率为 5 5 %。结论　松果体切除和松果体蒂部     

Electrical impedance scanning in breast tumor imaging: correlation with the growth pattern of lesion

Background This study researched the electric impedance properties of breast tissue and demonstrated the differentcharacteristic of electrical impedance scanning (EIS) images.Methods The impedance character of 40 malignant tumors, 34 benign tumors and some normal breast tissue from 69patients undergoing breast surgery was examined by EIS in vivo measurement and mammography screening, with aseries of frequencies set between 100 Hz-100 kHz in the ex vivo spectroscopy measurement.Results Of the 39 patients with 40 malignant tumors, 24 showed bright spots, 11 showed dark areas in EIS and 5showed no specific image. Of the 30 patients with 34 benign tumors there were almost no specific abnormality shown inthe EIS results. Primary ex vivo spectroscopy experiments showed that the resistivity of various breast tissue take thefollowing pattern: adipose tissue＞cancerous tissue＞mammary gland and benign tumor tissue.Conclusions There are significant differences in the electrical impedance properties between cancerous tissue andhealthy tissue. The impedivity of benign tumor is lower, and is at the same level with that of the mammary glandulartissue. The distinct growth pattern of breast lesions determined the different electrical impedance characteristics in theEIS results.     

平喘合剂治疗小儿哮喘发作期的临床研究

认为哮喘是外因作用于内因的结果 ,痰瘀伏肺为其宿根 ,外邪引触伏痰、痰瘀胶结、阻塞气道、痰阻气逆为其病机 ,寒热夹杂为其病性。具有宣肺化痰、降气平喘、清热活血功效的中药平喘合剂可明显改善哮喘发作期患儿的肺通气功能 ,明显降低患儿血浆中sICAM 1、IgE水平 ,与对照组比较有显著性差异 (P <0 .0 1,P <0 .0 5 ) ,推测其作用机制可能与减轻气道阻塞、调节免疫反应及抑制气道炎症等多个环节有关     

Effect of chronic selenite supplementation on selenium excretion and organ accumulation in rats

We examined the effect of chronic selenite supplementation on whole body and selected organ selenium (Se) accumulation, urine excretion of total Se and trimethylselenonium ion, and Se balance in adult male rats. Animals were housed in metabolic cages and given either deionized water or water containing 4 micrograms of Se/mL as selenite for 30 d. Absorption of selenite was nearly complete, with only approximately 10% of ingested Se appearing in feces. There was a rapid rise in urinary Se that reached a plateau within a few days and accounted for 54 +/- 2% of the intake. Excretion of trimethylselenonium ion (TMSe) in urine increased rapidly, representing 35-40% of urinary Se in the supplemented animals compared with only 2% for the control group. In one experiment, rats were killed at 30 d and total carcass Se was measured using isotope dilution analysis. Supplemented rats had only a modest increase in whole body Se (94 +/- 4 micrograms Se vs. 66 +/- 3 in controls). Calculation of Se balance in the supplemented rats showed that approximately 35% of ingested Se could not be accounted for by urine plus fecal losses combined with the portion retained in the carcass. The results from this study demonstrate that under the condition of supplementation at 4 micrograms of Se/mL of drinking water, pathways other than urinary and fecal excretion may account for a substantial portion of Se loss.     

Endotracheal suctioning causes right upper lobe collapse in intubated children

Objective: Right upper lobe collapse is a common radiographic finding in intubated children. We hypothesized that deep suctioning and uncontrolled negative pressures during endotracheal tube suctioning were significant contributory factors. Methods : The incidence of right upper lobe (RUL) collapse in intubated, ventilated children on a paediatric cardiac intensive care unit was determined over a 3-month period ( n = 102). Graduated suction catheters and suction vacuums of < 165 cm H₂O were then introduced. Another prospective audit was carried out 3 months later ( n = 60). Results : We found that 24% developed RUL collapse and 4 developed an apical pneumothorax. Following the introduction of graduated catheters and controlled vacuums pressures, a significant reduction in the incidence of RUL collapse, to 7%, was observed ( p < 0.05). Conclusions : We conclude that high negative pressure and deep-suctioning causes RUL collapse in children. Any lobar collapse not only prolongs the child's stay in intensive care, but can be associated with further morbidity which may have a serious implication. By improving suctioning technique this morbidity can be significantly reduced.     

自主神经功能与功能性便秘患者临床症状的相关性分析

目的：根据功能性便秘（functional constipation,FC）患者临床资料分析内脏自主神经功能与便秘症状特征、心理状态及生活质量的相关性。方法：评估102例FC患者和42例健康对照者的一般资料、精神心理状态和心率变异性（heart rate variability,HRV）检测结果,再根据HRV检测结果将FC患者分为自主神经张力偏向正常组、严重偏向交感神经张力组和严重偏向副交感神经张力组;比较各组间Zung’s焦虑自评量表（self-rating anxiety scale,SAS）和抑郁自评量表（self-rating depression scale,SDS）、便秘评分系统（constipation scoring system,CSS）和便秘患者症状自评量表（patient assessment of constipation symptoms, PAC-SYM）、便秘患者生活质量自评问卷（patient assessment of constipation quality of life,PAC-QOL）分值差异,同时比较不同 HRV分组间肛直肠测压参数、肠电图参数差异,分析便秘患者生活质量与自主神经功能、精神心理状态、便秘症状之间的相关性。结果：与健康对照组相比,FC患者焦虑抑郁水平显著升高,HRV检测结果显示自主神经功能异常率高。不同HRV分组的 FC患者间肛直肠测压参数、肠电图参数差异无统计学意义;与交感-副交感神经张力偏向正常组相比,严重偏向交感神经张力组患者明显存在明显的焦虑抑郁情绪、PAC-SYM较高、生活质量降低（主要是躯体不适和担心焦虑两个维度）。严重偏向副交感神经张力组患者CSS评分最高,可能与抑郁情绪为主导有关。多重线性回归显示,患者的焦虑抑郁情绪、自主神经功能显著影响便秘患者生活质量。结论：自主神经功能紊乱的便秘患者存在明显的焦虑抑郁情绪,与便秘严重程度相比,自主神经功能紊乱、焦虑抑郁情绪对便秘患者的生活质量具有更明显的负面影响。调节自主神经功能、心理干预或中枢神经药物等治疗可能具有重要临床意义。     

LncRNA VIM-AS1通过miR-497-5p/FBXW7轴调控高糖环境下视网膜内皮细胞的迁移和凋亡

目的：探讨长链非编码RNA(lncRNA)VIM反义RNA 1(VIM-AS1)在糖尿病视网膜病变中的潜在分子机制。方法：使用q RT-PCR测定LncRNA VIM-AS1、miR-497-5p和FBXW7 mRNA的表达。使用蛋白质印迹检测FBXW7蛋白水平。分别使用CCK-8实验、伤口愈合实验和流式细胞技术分析评估细胞增殖、迁移和凋亡。通过双荧光素酶报告基因分析验证lncRNA VIM-AS1、miR-497-5p和FBXW7之间的结合关系。结果：在高糖处理的ARPE-19细胞中,LncRNAVIM-AS1和FBXW7的表达显著降低,而miR-497-5p的表达上调。LncRNA VIM-AS1可以通过竞争性结合miR-497-5p上调FBXW7的表达。LncRNA VIMAS1过表达能够促进HG处理的ARPE-19细胞的增殖和迁移,并抑制细胞凋亡,而miR-497-5p过表达消除了lncRNA VIM-AS1过表达对HG处理的ARPE-19细胞的影响。此外,FBXW7敲低消除了miR-497-5p对HG处理的ARPE-19细胞表型的抑制。结论：lncRNA VIM-AS1可通过...     

慢加急性肝衰竭诊断标准的研究进展

慢加急性肝衰竭(ACLF)是指在慢性肝病基础上遭受急性打击后,出现严重的急性肝功能失代偿,其因病情进展迅速、短期死亡率极高,引起了全球肝病学家的关注。但由于不同国家(地区)ACLF的病因及临床特征等存在明显的差异,目前国内外ACLF的诊断标准多达十余种。本文通过回顾几种较为常用的ACLF定义,以及ACLF诊断标准相关研究,比较各ACLF诊断标准之间的差异,以期帮助临床医生更好地识别ACLF、优化临床决策。目前全球尚无公认的ACLF诊断标准,国内外ACLF的诊断标准存在着较大差异,临床医生在实际工作中选用何种ACLF诊断标准应根据患者慢性肝病的病因、临床特征等因素综合考虑。     

芪苈强心胶囊联合尼可地尔对射血分数保留性心力衰竭患者心功能及细胞因子的影响

目的 探讨芪苈强心胶囊联合尼可地尔对射血分数保留性心力衰竭患者心功能及细胞因子的影响。方法 连续入选2019年10月至2020年10月诊断为射血分数保留性心力衰竭的患者共90例,随机分为观察组(45例)与对照组(45例)。对照组给予常规抗心力衰竭药物加芪苈强心胶囊治疗,观察组在对照组治疗基础上,加用尼可地尔治疗。比较两组患者一般临床资料、细胞因子水平、心功能各项指标。随访1年患者心功能情况、细胞因子水平以及不良心脑血管事件(MACCEs)发生情况。结果 两组患者接受治疗1年后脑钠肽(BNP)、左室舒张末期内径、白细胞介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)均降低,射血分数(EF)值较治疗前升高,差异有统计学意义(P<0.05)。组间对比发现,两组患者入院时基础BNP、细胞因子、超声心能指标(LVEF、左室舒张末期内径、左房内径)差异无统计学意义(P>0.05);接受治疗1年后观察组IL-1β、IL-6、TNF-α、BNP、左室舒张末期内径低于对照组,EF值高于对照组,差异有统计学意义(P<0.05)。随访1年对照组共有7例发生终点事件,观察组共有4...