CUDR promotes liver cancer stem cell growth through upregulating TERT and C-Myc

Cancer up-regulated drug resistant (CUDR) is a novel non-coding RNA gene. Herein, we demonstrate excessive CUDR cooperates with excessive CyclinD1 or PTEN depletion to accelerate liver cancer stem cells growth and liver stem cell malignant transformation in vitro and in vivo. Mechanistically, we reveal the decrease of PTEN in cells may lead to increase binding capacity of CUDR to CyclinD1. Therefore, CUDR-CyclinD1 complex loads onto the long noncoding RNA H19 promoter region that may lead to reduce the DNA methylation on H19 promoter region and then to enhance the H19 expression. Strikingly, the overexpression of H19 increases the binding of TERT to TERC and reduces the interplay between TERT with TERRA, thus enhancing the cell telomerase activity and extending the telomere length. On the other hand, insulator CTCF recruits the CUDR-CyclinD1 complx to form the composite CUDR-CyclinD1-insulator CTCF complex which occupancied on the C-myc gene promoter region, increasing the outcome of oncogene C-myc. Ultimately, excessive TERT and C-myc lead to liver cancer stem cell and hepatocyte-like stem cell malignant proliferation. To understand the novel functions of long noncoding RNA CUDR will help in the development of new liver cancer therapeutic and diagnostic approaches.     

Group-Wise FMRI Activation Detection on DICCCOL Landmarks

Group-wise activation detection in task-based fMRI has been widely used because of its robustness to noises and its capacity to deal with variability of individual brains. However, current group-wise fMRI activation detection methods typically rely on the co-registration of individual brains’ fMRI images, which has difficulty in dealing with the remarkable anatomic variation of different brains. As a consequence, the resulted misalignments could significantly degrade the required inter-subject correspondences, thus substantially reducing the sensitivity and specificity of group-wise fMRI activation detection. To deal with these challenges, this paper presents a novel approach to detecting group-wise fMRI activation on our recently developed and validated Dense Individualized and Common Connectivity-based Cortical Landmarks (DICCCOL). The basic idea here is that the first-level general linear model (GLM) analysis is first performed on the fMRI signal of each corresponding DICCCOL landmark in individual brain’s own space, and then the estimated effect sizes of the same landmark from a group of subjects are statistically assessed with the mixed-effect model at the group level. Finally, the consistently activated DICCCOL landmarks are determined and declared in a group-wise fashion in response to external block-based stimuli. Our experimental results have demonstrated that the proposed approach can detect meaningful activations.     

超声内镜对克罗恩病的诊断价值

目的探讨超声内镜在克罗恩病（CD）诊断中的应用价值。方法对436例内镜下疑似CD患者行内镜超声（EUS）检查,统计EUS诊断结果与临床确诊结果的一致性。结果临床确诊CD297例、非CD139例,EUS诊断CD277例（其中17例临床确诊为非CD）、非CD159例（其中37例临床确诊为CD）,EUS诊断CD的敏感度、特异度和准确率分别为87．5％（260／297）、87．8％（122／139）和87．6％（382／436）。另外,297例确诊CD患者中,EUS下发现黏膜下层血管扩张40例,瘘道13例,脓肿5例,探及管壁外肿大淋巴结75例。结论EUS下可对CD的消化道层次进行清晰观察,诊断准确率较高。同时,EUS能很好地发现瘘道、脓肿等肠外并发症,可为外科治疗提供有价值的信息。     

中西医结合对慢性心力衰竭的现代认识

总结中西医结合学界对慢性心力衰竭的实验研究和临床研究，进一步认识其在病因、病机、病理、病程发展及辨证分型等方面的研究现状。     

前额皮瓣鼻再造术13例报告

目的：介绍应用额部皮瓣进行鼻再造的体会。方法：对8例外鼻皮肤癌术后缺损及5例鼻外伤后缺损患者采用额部皮瓣转移行鼻再造。结果：13例患者转移额部皮瓣全部成活,切口Ⅰ期愈合,无并发症发生。随访1～17年,鼻外形满意,肿瘤无复发。结论：应用额部皮瓣进行鼻再造,具有操作方便、血供可靠、成活率高、效果满意等优点,有临床应用价值。     

培养海马神经元突触外NMDA受体通道电流在发育中的变化

为观察培养大鼠海马神经元突触外NMDA受体通道电流在发育中的变化,本研究采用膜外面向外模式记录突触外NMDA受体介导的单通道电流。结果显示:培养2周神经元的电流幅度和开放概率比培养1周神经元大,但电导和翻转电位无显著差异。培养2周神经元只出现高电导开放,培养1周神经元同时出现高电导和低电导两种开放形式。NR2B受体亚型的特异性拮抗剂ifenprodil可降低培养1周和2周海马神经元的电流幅度、电导和开放概率,且对培养2周神经元开放概率的抑制作用更明显。以上结果表明,培养海马神经元突触外NMDA受体通道电流有发育变化,且培养1周神经元突触外NMDA受体的NR2亚型可能为NR2B和NR2D;而神经元培养到2同时,突触外主要为NR2B亚型,且数量有所增加。     

广西某医学院校女大学生婚前性行为认知现状

目的 了解广西某医学院校女大学生婚前性行为的发生情况、认知水平和影响因素,为广西高校大学生性健康教育工作提供理论依据。方法 采用方便抽样方法对广西某医学院校499名在校女大学生进行问卷调查,用描述性分析、卡方检验和Logistics回归分析方法进行统计分析。结果 有69名女大学生存在婚前性行为,其发生率为13.83%。其中,非常了解避孕工具正确使用方法者仅占55.1%,有63.8%的女大学生在进行性行为时,每次都会要求对方使用安全套。不同年级、家庭类型和现阶段不同感情状态的婚前性行为的发生率差异均存在统计学意义（P﹤0.05）。Logistics分析结果显示：有对象、经常主动在网上浏览“性”相关信息、父母对婚前性行为呈支持态度和周围有发生婚前性行为的同龄人是女大学生婚前性行为的危险因素。结论 广西某医学院校女大学生的性健康知识存在薄弱环节,婚前性行为较常见。学校应加强性健康相关知识教育,帮助其树立正确的性观念和性态度,营造良好的周围环境,减少高危性行为的发生。