全髋关节翻修术（48例50髋随诊分析）

目的评价人工全髋关节翻修术临床效果。方法自1984年1月～2006年4月,56例58髋行髋关节翻修术,男22例,女34例,年龄36～73岁,平均63岁。初次人工髋关节置换前诊断为股骨颈骨折28例,股骨头缺血坏死18例,髋关节发育不良骨关节炎9例,强直性脊柱炎1例。初次行股骨头置换术9例,其中骨水泥2例,生物型固定7例。全髋关节置换47例49髋。其中骨水泥30髋,生物型固定19髋。术后早期脱位2例,16髋因髋关节严重疼痛,45例47髋因无菌性松动行全关节翻修术。翻修手术中28髋行髋臼植骨,其中自体碎屑植骨17例18髋。自体碎骨屑加异体大块植骨10例。结果资料完整48例50髋获得随访,最长20年,最短4个月,平均随访时间为5年。经随访,患者髋关节基本无疼痛,X线片显示髋臼和股骨柄假体位置满意,无松动和感染征象,髋臼植骨后愈合良好。4例股骨柄假体正位X线片仅1区出现约1mm宽的透亮带,临床检查无松动迹象。Harris评分由术前的平均41.3分(10～70分)提高到术后的平均80.4分(55～97分)。结论全髋关节翻修手术的病例经随访,临床达到满意效果。     

桃核承气汤及单味大黄中大黄酸在家兔体内的药代动力学

目的:比较家兔灌服桃核承气汤和单味大黄后大黄酸的药代动力学特征.方法:家兔灌服桃核承气汤5.0g生药/kg和单味大黄1.4g生药/kg后,血浆经高氯酸和乙醚萃取,用高效液相色谱法测定大黄酸血药浓度.色谱柱为Eclipse XDB-C18柱,流动相为乙腈-0.1%磷酸,紫外检测波长为258nm.结果:家兔灌服桃核承气汤和单味大黄后,大黄酸血浆浓度-时间曲线符合二室开放模型.桃核承气汤中大黄酸吸收更为迅速,按相同给药剂量计(1 mg/kg),灌服桃核承气汤和单味大黄大黄酸的Cmax分别为(0.682±0.142)mg/L、(0.358±0.105)mg/L,二者差异有显著意义(P<0.01).结论:桃核承气汤药味配伍促进了大黄酸的吸收利用.     

苯醋酸抗脑胶质瘤颅内移植实验研究

目的：通过苯醋酸对脑胶质瘤小鼠模型的诱导治疗,观察治疗后肿瘤细胞的变化,以评估其在体内治疗恶性脑肿瘤的效果。方法：将Ｇ４２２鼠脑胶质瘤细胞移植到昆明鼠（ｎ＝８０）脑内,并将其平均分为５组。其中３组为苯醋酸钠用药组,分别用１２００、８００、４００ｍｇ·ｋｇ１·ｄ１,自移植后的当天开始用药,连续用药１４ｄ;对照组用同样的方法注射等量的盐水;另一组为卡氮芥（ＢＣＮＵ）组,２０ｍｇ·ｋｇ１仅１次注射。治疗结束后处死小鼠,行病理、电镜和毒性观察以及流式细胞仪检查,以确定肿瘤细胞的增殖状态。结果：用苯醋酸治疗的小鼠生存期均有延长（Ｐ＜０．０５）,而没有反应。ＢＣＮＵ组的生命延长期和用苯醋酸治疗的中剂量组相同。病理和电镜观察显示：用苯醋酸处理的小鼠肿瘤细胞有明显的细胞肥大和粗面内质网的机化,表示有细胞的分化。流式细胞仪检测显示,苯醋酸可以使Ｇ４２２细胞的分化阻滞在Ｇ１期。结论：醋酸钠通过抑制蛋白的戊乙烯化或其它机制,而达到抑制肿瘤细胞增殖和诱导分化的作用,可以作为治疗恶性胶质瘤或其它恶性肿瘤的安全有效的新药。     

急性分离大鼠新皮层神经元KATP通道特性

为研究急性分离大鼠皮层神经元上ＡＴＰ敏感钾通道的特性,本实验采用了膜片钳技术之膜内面向外记录方法。ＡＴＰ敏感钾通道电导为２００ｐＳ左右,翻转电位为０ｍＶ,有外向整流现象;通道开放常呈簇状猝发样开放,平均开放时间和开放概率随去极化程度增大而增加,随超极化程度增大而减少;低浓度ＡＴＰ有促进通道开放作用,并可激活二级电流;高浓度ＡＴＰ抑制通道开放概率并随浓度增大而增强;ＡＴＰ浓度增高到１或２ｍｍｏｌ／Ｌ时,通道电流被阻断。０.１ｍｍｏｌ／Ｌ优降糖可立即抑制通道活动。急性分离的皮层神经元上ＡＴＰ敏感钾通道特性与培养神经元有差别;低浓度ＡＴＰ可激活ＡＴＰ敏感钾通道,防止神经细胞过度兴奋,从而起到保护脑缺血缺氧损伤的作用。     

对精神分裂症实施心理治疗前后比较研究

为了探索缓解期精神分裂症心理治疗的方法与疗效，以ＳＣＬ－９０症状自评量表和自编简明心理行为量表为量化指标，对４３例患者作了心理治疗前后的比较研究。结果表明，接受心理治疗一年后的２７例患者与未接受心理治疗前的４３例患者，ＳＣＬ－９０总均分及１０个因子中的９个因子均分有明显差异。简明心理行为量表１３种心理行为有明显改变。     

大鼠肺癌模型诱癌前处周血淋巴细胞染色体改变过程的研究

目的：观察肺癌模型诱癌前外周血淋巴细胞染色体。方法：对10只Sprague-Dewely大鼠心脏取血，采用常规染色体制作方法及分带技术。结果：染色体众数为42，即20对常染色体和1对性染色体。其中4对为近端着丝粒染色体，7对为中着丝粒染色体，10对为顶端着丝粒染色体。结论：诱癌前实验大鼠染色体的研究为其与诱癌过程中染色体的改变对比提供依据。     

动静脉溶栓治疗早期脑梗塞的对比研究

目的探索早期脑梗塞的有效疗法。方法选择发病6h内脑梗塞患者93例，随机分为动脉组（32例）和静脉组（61例）。除一般治疗外，静脉组予以尿激酶25万U／d静脉滴注，连续7d。动脉组经股动脉穿刺，将多孔微导管插入血栓，按1万U／min注入尿激酶，每使用25万U即测凝血指标并行血管造影，血管开通后，再注入25万U以溶解迁徙性血栓。结果两组3个月后疗效显示，动脉组有效率为87．5％，优于静脉组的60．65％（P〈0．05）；日常生活能力评价动脉组亦优于静脉组（P〈0．05）。结论采用动脉溶栓治疗早期脑梗塞的疗效优于静脉溶栓。     

Dynamic brain connectivity is a better predictor of PTSD than static connectivity

Using resting‐state functional magnetic resonance imaging, we test the hypothesis that subjects with post‐traumatic stress disorder (PTSD) are characterized by reduced temporal variability of brain connectivity compared to matched healthy controls. Specifically, we test whether PTSD is characterized by elevated static connectivity, coupled with decreased temporal variability of those connections, with the latter providing greater sensitivity toward the pathology than the former. Static functional connectivity (FC; nondirectional zero‐lag correlation) and static effective connectivity (EC; directional time‐lagged relationships) were obtained over the entire brain using conventional models. Dynamic FC and dynamic EC were estimated by letting the conventional models to vary as a function of time. Statistical separation and discriminability of these metrics between the groups and their ability to accurately predict the diagnostic label of a novel subject were ascertained using separate support vector machine classifiers. Our findings support our hypothesis that PTSD subjects have stronger static connectivity, but reduced temporal variability of connectivity. Further, machine learning classification accuracy obtained with dynamic FC and dynamic EC was significantly higher than that obtained with static FC and static EC, respectively. Furthermore, results also indicate that the ease with which brain regions engage or disengage with other regions may be more sensitive to underlying pathology than the strength with which they are engaged. Future studies must examine whether this is true only in the case of PTSD or is a general organizing principle in the human brain. Hum Brain Mapp 38:4479–4496, 2017. © 2017 Wiley Periodicals, Inc.     

CD99 refers to the activity of inflammatory bowel disease

Background: Inflammatory bowel disease (IBD), composed of Crohn’s disease (CD) and ulcerative colitis (UC), is an inflammatory autoimmune disease. CD99 has been reported to participate in migration of leukocytes and T cell activation. However, the roles of CD99 in IBD are obscure.

Materials and methods: CD99 expression was examined in peripheral blood mononuclear cells (PBMCs) and inflamed mucosa from IBD patients by qRT-PCR. Serum TNF-α and IL-17A levels were detected by ELISA. Correlations of CD99 expression with TNF-α, IL-17A, Crohn’s disease activity index (CDAI), simple endoscopic score for CD (SES-CD), Mayo index, and Truelove grading were performed by Pearson’s correlation.

Results: CD99 expression was increased in PBMCs and inflamed mucosa from active CD and UC patients, and CD99 expression was also increased in the inflamed mucosa compared with unaffected control from the same patients. Serum TNF-α and IL-17A levels were increased in active CD or UC patients, and positively correlated with CD99 expression in PBMCs (CD: r?=?.402, p?=?.009; r?=?.350, p?=?.025. UC: r?=?.289, p?=?.028; r?=?.322, p?=?.014). Moreover, CD99 expression in inflamed mucosa was correlated with CDAI, SES-CD, Mayo index, and Truelove grading (r?=?.410, p?=?.012; r?=?.341, p?=?.005; r?=?.366, p?=?.002; r?=?.312, p?=?.011).

Conclusion: CD99 expression is increased in patients with active IBD, and positively correlated with disease activity. Therefore, CD99 expression can be used as an index to evaluate the activity of IBD.