NO与肝癌形成

目的研究一氧化氮(NO)在肝癌发生发展的作用。方法用免疫组化的方法对21例肝癌及癌旁组织中的3种一氧化氮合酶(NOS)及血管内皮细胞生长因子(VEGF)的表达进行原位检测和观察,结果紧邻癌细胞的肝硬化组织或慢性肝炎组织iNOS呈强阳性,远离癌组织的肝硬化组织或慢性肝炎组织多呈阴性或弥漫弱阳性;iNOS在周边癌组织及侵入纤维组织中的癌细胞呈阳性,癌组织核心多呈阴性或弥漫弱阳性。VEGF、nNOS的分布与iNOS相似。eNOS主要分布在肝癌细胞小血管壁内皮及其肌层组织。结论 NOS表达与肝组织癌变及肝癌侵润能力有关,与癌组织获得血管形成和转移表型有关。     

急性分离大鼠新皮层神经元KATP通道的多电导状态

在１９３例急性分离大鼠皮层神经元内面向外式记录膜片上，记录到２例能为ＡＴＰ和优降糖所阻断的含有亚电导的通道电流。其电流幅度分布直方图符合高斯分布，并能很好地进行二级拟合；主电导水平分别为１９１．８ｐＳ和１９４．５ｐＳ，亚电导水平分别为１５３．２ｐＳ和１１６.１ｐＳ；亚电导成份分别占单位事件总数的３５．０５％和２９.８４％；通道活动呈高电流水平与低电流水平交替开放状态，没有高低电流叠合现象。这些结果表明这种Ｋ_ＡＴＰ通道的多电导开放电流不是两个或多个独立通道的开放所致，而是同一通道的不同亚型状态交互活动（即高电导和低电导状态相互转换）的表现。从而提示神经细胞膜上的Ｋ_ＡＴＰ通道可能受某种或某些膜内外环境的变化影响导致其构象改变，从而引发多种亚型交替活动现象。     

卡介菌多糖核酸对变应性鼻炎的免疫调节及临床疗效观察

目的:观察卡介菌多糖核酸(BCG-PSN)对常年性变应性鼻炎患者的免疫调节作用及临床疗效。方法:将60例常年性变应性鼻炎患者随机分为试验组和对照组,每组30例。试验组在与对照组相同治疗的基础上给予卡介菌多糖核酸注射液1ml(0.5mg),肌肉注射,隔日1次,疗程为6周。两组均随访3个月,每2周门诊随访1次,对临床情况进行评价,并检测两组患者治疗前、后血清IgA、IgG、IgM、IgE、CD3、CD4及CD8水平。结果:试验组有效率为93.33%,对照组为63.33%,其差异有统计学意义(P<0.05);卡介菌多糖核酸治疗后CD3、CD4、CD4/CD8水平均明显升高,分别与治疗前及对照组的治疗后比较,差异有统计学意义(均P<0.05)。治疗后IgA、IgM和IgE明显降低,与治疗前比较,差异均有统计学意义(均P<0.05),其中IgE与对照组的治疗后比较,差异亦有统计学意义(P<0.05)。结论:卡介菌多糖核酸可调节常年性变应性鼻炎患者的免疫功能,与抗过敏药物联合应用可提高其疗效,具有临床实用价值。     

Alcohol Sensor Based on Surface Plasmon Resonance of ZnO Nanoflowers/Au Structure

Alcohol detection plays a key role in food processing and monitoring. Therefore, we present a fast, high reproducibility and label-free characteristics alcohol photochemical sensor based on the surface plasmon resonance (SPR) effect. By growing ZnO nanoflowers on Au film, the SPR signal red-shifted in the visible region as the alcohol concentration increased. More interestingly, the sensitivity improved to 127 nm/%, which is attributed to the ZnO nanoflowers/Au structure. The goodness of the linear fit was more than 0.99 at a range from 0 vol% to 95 vol% which ensures detection resolution. Finally, a practical application for distinguishing five kinds of alcoholic drinks has been demonstrated. The excellent sensing characteristics also indicate the potential of the device for applications in the direction of food processing and monitoring, and the simple structure fabrication and economic environmental protection make it more attractive.     

运用通督填髓法治疗痿证

总结运用通督填髓法治疗痿证的经验.督脉通过与人体六阳经、阳维脉、阳跷脉及带脉的脉络交汇联系,建立了与脑窍、四肢百骸及肌肉筋脉之间的重要通道,其可上输精髓以充养脑窍、调动诸经的阳气及精微物质输注、荣养宗筋.基于此,认为痿证的病因主要为督脉不通、脑窍失养,治疗主张"治痿必治督",运用引经药以通行督脉,再佐以补精填髓之品,共...     

Familial hemophagocytic lymphohistiocytosis in a neonate: Case report and literature review

Rationale:Familial hemophagocytic lymphohistiocytosis (FHL) is a potentially fatal disease that rarely presents in the neonatal period. Timely diagnosis is a key challenge owing to the atypical clinical manifestations. Here, we describe a case of FHL type 3 with disease onset in the early neonatal period and review the relevant literature. Our findings may provide insights into the diagnosis and treatment of this rare disease.Patient concerns:A 6-day-old male neonate presented with fever, hepatosplenomegaly, cytopenia, hyperferritinemia, hypofibrinogenemia, hemophagocytosis, and hypertriglyceridemia.Diagnosis:Considering the clinical picture (prolonged fever, progressive hepatosplenomegaly, high triglycerides, low fibrinogen, and high ferritin), along with abnormal natural killer-cell activity, combining sequence analysis of genomic DNA results (compound heterozygous mutations of UNC13D), the patient was finally diagnosed with FHL type 3 (FHL3).Interventions:The patient was initially treated with HLH-1994 protocol and subsequently switched to an oral regimen of ruxolitinib due to incomplete remission of the disease.Outcomes:The trend of change in weekly cytokine levels, neutrophil counts, hemoglobin, and platelet counts indicated that the complete remission was not achieved after the treatment of HLH-1994 protocol. The platelet counts fluctuated within the normal range after oral administration of ruxolitinib. But soon after, the patient did not respond to treatment and eventually died of respiratory failure.Lesson:Timely diagnosis of FHL is challenging. This case report illustrates that thrombocytopenia can be the first clinical sign of FHL with neonatal onset. Genetic testing, detection of cytokines, and flow cytometry should be performed as soon as possible to confirm the diagnosis. Given the high morbidity and mortality of FHL, pediatricians should have a high suspicion index for this disease.     

慢性前列腺炎诊断和治疗的再评价

目的探讨慢性前列腺炎诊断流程简化的可行性.方法慢性前列腺炎患者80例,随机分为研究组和对照组.研究组治疗前作EPS细菌培养,并在治疗前、治疗完成日和治疗完成后1个月时分别进行EPS常规检查.对照组不进行EPS的各项相关检查.患者需要填写NIH-CPSI症状评分.结果研究组与对照组在治疗前、后的症状改变明显(P＜0.01).研究组与对照组间无明显统计学差异.停药30天后,无明显复发.停药后30天白细胞略有升高,但无统计学差异(P＞0.05).结论适当简化慢性前列腺炎诊断检验流程是必要和可行的.     

食色一体

“食”与“色”是人类的两大本能，从理论上将其分开，是从春秋时代才开始的文明人的习惯。本文运用考古资料和《诗经》证明，最迟从６０００年前的抑韶文化时代开始，一直到东周时代，“食色一体”都是中国的基本风俗，从而从一个侧面揭示了中国生殖文化的部分特征。     

口服伐地那非治疗勃起功能障碍疗效和安全性的临床研究

目的 :评价伐地那非对男性勃起功能障碍 (ED)患者的疗效和安全性。　方法 :应用随机、双盲、安慰剂平行对照、剂量固定 (5、1 0和 2 0mg)方法 ,对 88例ED患者进行 1 2周的临床研究。　结果 :5、1 0和 2 0mg伐地那非使ED患者达到和维持勃起的临床主要和次要指标均明显高于安慰剂 (P <0 .0 1 ) ;伐地那非各剂量组不良事件发生率高于安慰剂组 ,均为轻至中度 ,呈一过性。　结论 :伐地那非是治疗各种病因导致ED的安全、有效的药物。     

16层螺旋CT在泌尿系疾病诊断中的应用价值

目的探讨16层螺旋CT在泌尿系疾病诊断中的应用价值。方法115例泌尿系病变病例,60例单纯行16层螺旋平扫,51例行四期扫描(平扫、皮质期、实质期、肾盂期,其中应用Sure-Start软件30例,常规增强21例),非增强尿路成像4例,利用后处理技术对扫描资料进行三维重建处理,可以得到包括肾盏、肾盂、输尿管及膀胱在内的完整尿路成像。结果16层螺旋CT能清晰显示泌尿系统的解剖结构,以及病变和尿路之间的空间关系、双肾功能情况。115例病人中,诊断先天发育畸形17例,胡桃夹现象2例,泌尿系结核10例,多囊肾3例,单纯囊肿10例,泌尿系结石58例,肾、输尿管、膀胱肿瘤15例。结论16层螺旋CT泌尿系扫描,对显示泌尿系病变部位、病因和尿路梗阻的程度及双肾功能,具有检查快速、后处理快捷、安全可靠的特点,可提供完整尿路图像,为临床的准确诊断提供依据。