全文获取类型
收费全文 | 664篇 |
免费 | 39篇 |
国内免费 | 4篇 |
专业分类
耳鼻咽喉 | 4篇 |
儿科学 | 23篇 |
妇产科学 | 10篇 |
基础医学 | 89篇 |
口腔科学 | 8篇 |
临床医学 | 114篇 |
内科学 | 148篇 |
皮肤病学 | 4篇 |
神经病学 | 24篇 |
特种医学 | 51篇 |
外科学 | 77篇 |
综合类 | 14篇 |
预防医学 | 67篇 |
眼科学 | 7篇 |
药学 | 39篇 |
肿瘤学 | 28篇 |
出版年
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 8篇 |
2019年 | 14篇 |
2018年 | 6篇 |
2017年 | 7篇 |
2016年 | 12篇 |
2015年 | 14篇 |
2014年 | 21篇 |
2013年 | 24篇 |
2012年 | 46篇 |
2011年 | 34篇 |
2010年 | 27篇 |
2009年 | 16篇 |
2008年 | 30篇 |
2007年 | 49篇 |
2006年 | 27篇 |
2005年 | 36篇 |
2004年 | 28篇 |
2003年 | 25篇 |
2002年 | 21篇 |
2001年 | 14篇 |
2000年 | 19篇 |
1999年 | 9篇 |
1998年 | 21篇 |
1997年 | 13篇 |
1996年 | 23篇 |
1995年 | 10篇 |
1994年 | 8篇 |
1993年 | 17篇 |
1992年 | 10篇 |
1991年 | 7篇 |
1990年 | 12篇 |
1989年 | 16篇 |
1988年 | 11篇 |
1987年 | 7篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 10篇 |
1983年 | 5篇 |
1982年 | 3篇 |
1980年 | 2篇 |
1979年 | 4篇 |
1978年 | 2篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1975年 | 2篇 |
1972年 | 6篇 |
1971年 | 3篇 |
1966年 | 2篇 |
排序方式: 共有707条查询结果,搜索用时 15 毫秒
31.
32.
33.
Qasim W King D Buddle J Verfuerth S Kinnon C Thrasher AJ Gaspar HB 《British journal of haematology》2003,123(4):712-719
Retroviral vectors encoding the herpes simplex thymidine kinase gene have been used to render T cells sensitive to the prodrug ganciclovir. Such genetically modified T cells have been used in clinical trials for their graft-versus-leukaemia effects following allogeneic haematopoietic stem cell transplantation. In the event of graft-versus-host disease (GVHD) the cells were susceptible to elimination through exposure to ganciclovir. We have investigated the impact of T-cell activation, required for successful retrovirus-mediated gene transfer, on T-cell receptor repertoire profile, subset distribution and antiviral potential. Using a combination of antibodies against CD3 and CD28, T cells were transduced at high efficiency when exposed to retrovirus between 48 and 72 h later. Lymphocytes had undergone up to seven cycles of cell division by the end of the procedure. Although the T-cell receptor Vbeta repertoire was not altered after retroviral transduction, there were notable shifts in subset profiles with an increased proportion of CD45RO cells in transduced populations. T cells continued to proliferate for several days after transduction and were difficult to sustain under the extended culture conditions required to generate virus-specific T cells. These observations may explain the lower than expected levels of GVHD and poor antiviral immunity reported in recent trials. 相似文献
34.
35.
36.
37.
A F Dean I Janota A Thrasher I Robertson G Mieli-Vergani 《Archives of disease in childhood》1993,68(3):412-414
A 2 year old girl presented with epilepsy 16 months after being diagnosed as having autosomal recessive chronic granulomatous disease. Computed tomography showed a cerebral mass which was surgically removed and proved histologically to be an aspergilloma. This case illustrates the application of molecular diagnostic techniques to the diagnosis of chronic granulomatous disease. The occurrence of, and unusual reaction to, cerebral aspergillus infection indicates the need to consider this possibility in the differential diagnosis of mass lesions in chronic granulomatous disease. Furthermore, it is clear that autosomal recessive chronic granulomatous disease cannot be considered to be a clinically mild form that is exempt from major neurological complications. 相似文献
38.
Chemotaxis of macrophages is abolished in the Wiskott-Aldrich syndrome 总被引:12,自引:0,他引:12
Zicha Allen Brickell Kinnon Dunn Jones & Thrasher 《British journal of haematology》1998,101(4):659-665
Wiskott-Aldrich syndrome (WAS) is a rare disease characterized by microthrombocytopenia, eczema and immune deficiency. In this study a direct-viewing chemotaxis chamber was used to analyse chemotactic responses of WAS neutrophils and macrophages in stable linear concentration gradients. In five patients with classic WAS, chemotaxis of macrophages but not of neutrophils was found to be abolished, whereas the speed of random motility of both cell types was found to be indistinguishable from control cells. This supports the existence of an essential functional link, previously suggested by biochemical studies, between Cdc42, WAS protein (WASp) and the actin cytoskeleton in primary human macrophages. Moreover, these data suggest that Cdc42-WASp-mediated filopodial extension is a requirement for chemotaxis but not for chemokinesis in these cells. Abnormal directional cell motility of macrophages and related antigen-presenting cells may play a significant part in the immune deficiency and eczema of WAS. 相似文献
39.
40.