全文获取类型
收费全文 | 2487篇 |
免费 | 181篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 22篇 |
儿科学 | 62篇 |
妇产科学 | 43篇 |
基础医学 | 345篇 |
口腔科学 | 27篇 |
临床医学 | 349篇 |
内科学 | 669篇 |
皮肤病学 | 26篇 |
神经病学 | 101篇 |
特种医学 | 56篇 |
外科学 | 105篇 |
综合类 | 22篇 |
现状与发展 | 1篇 |
预防医学 | 360篇 |
眼科学 | 43篇 |
药学 | 273篇 |
中国医学 | 29篇 |
肿瘤学 | 141篇 |
出版年
2024年 | 5篇 |
2023年 | 42篇 |
2022年 | 137篇 |
2021年 | 192篇 |
2020年 | 92篇 |
2019年 | 104篇 |
2018年 | 89篇 |
2017年 | 57篇 |
2016年 | 79篇 |
2015年 | 76篇 |
2014年 | 75篇 |
2013年 | 114篇 |
2012年 | 178篇 |
2011年 | 140篇 |
2010年 | 95篇 |
2009年 | 79篇 |
2008年 | 147篇 |
2007年 | 146篇 |
2006年 | 98篇 |
2005年 | 123篇 |
2004年 | 85篇 |
2003年 | 98篇 |
2002年 | 67篇 |
2001年 | 34篇 |
2000年 | 31篇 |
1999年 | 26篇 |
1998年 | 19篇 |
1997年 | 13篇 |
1996年 | 13篇 |
1995年 | 8篇 |
1994年 | 8篇 |
1993年 | 9篇 |
1992年 | 18篇 |
1991年 | 14篇 |
1990年 | 16篇 |
1989年 | 14篇 |
1988年 | 21篇 |
1987年 | 11篇 |
1986年 | 9篇 |
1985年 | 17篇 |
1984年 | 7篇 |
1983年 | 7篇 |
1982年 | 6篇 |
1981年 | 4篇 |
1978年 | 6篇 |
1977年 | 7篇 |
1975年 | 4篇 |
1969年 | 7篇 |
1967年 | 6篇 |
1966年 | 5篇 |
排序方式: 共有2674条查询结果,搜索用时 15 毫秒
91.
Vincent Dellière N. Bertheuil Y. Harnois S. Thiénot M. Gérard M. Robert E. Watier 《Indian Journal of Plastic Surgery》2014,47(3):436-440
We present a rare complication of abdominal liposuction: bowel perforation and necrotizing fasciitis. Because of bilateral lumbar hernia, a 56-year-old woman had caecum and descending colon perforation during lipoplasty. She had septic shock syndrome at her admission. The authors treated this complex wound with several debridement, omental flap, NPWT and split-thickness skin graft. The incidence of abdominal wall perforation with visceral injury is 14 in 100,000 liposuctions performed. There are only 12 cases of bowel perforation in literature but this complication is probably underestimated. Prompt surgical debridement is absolutely mandatory in this life threatening scenario. Lumbar hernia is very rare and should be ruled out before every abdominal liposuction clinically or with imaging modalities.KEY WORDS: Bowel perforation, liposuction, lumbar hernia, necrotizing fasciitis, negative pressure large wound therapy 相似文献
92.
Hue Thi Truong Van-Anh Thi Nguyen Hong-Loan Thi Nguyen Van-Anh Pham Tuan-Nghia Phan 《Central European Journal of Medicine》2014,9(6):839-848
The A3243G mitochondrial mutation is the major cause of mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS). The severity of the disease is correlated with the heteroplasmy level of the mutation. Here we describe for the first time the validation of a real-time polymerase chain reaction (PCR) assay with Taqman locked nucleic acid (LNA) fluorescent (FAM for mutant, HEX for wild type) probes for quantification of heteroplasmy levels in a total of 18 family members from 5 Vietnamese MELAS patients carrying A3243G. Almost no background of FAM signals was detected in normal samples, indicating that the probes were allele-specific. Standard curves indicate sensitive detection at 0.1% mutants and high reliability with R2 > 0.985. The correlation line between measured % mutant and expected % mutant was highly reliable, with a slope of 0.993 and R2 of 0.998. All positive A3243G mutant samples pre-screened by PCR-restriction fragment length polymorphism (RFLP) were confirmed, and their heteroplasmy levels quantified to be from 3.68 to 80.85%. The heteroplasmy levels in patients were higher than in their family members and generally correlated well with the severity of their clinical symptoms. Overall, this work is the first demonstration of the application of LNA probes for sensitive and highly reliable quantification of heteroplasmy levels in human mitochondria. 相似文献
93.
94.
95.
96.
Nguyen Thanh Si Nguyen Thi Ai Nhung Thanh Q. Bui Minh Tho Nguyen Pham Vu Nhat 《RSC advances》2021,11(27):16619
Pramipexole (PPX) is known in the treatment of Parkinson''s disease and restless legs syndrome. We carried out a theoretical investigation on pramipexole–Au cluster interactions for the applications of drug delivery and detection. Three AuN clusters with sizes N = 6, 8 and 20 were used as reactant models to simulate the metallic nanostructured surfaces. Quantum chemical computations were performed in both gas phase and aqueous environments using density functional theory (DFT) with the PBE functional and the cc-pVDZ-PP/cc-pVTZ basis set. The PPX drug is mainly adsorbed on gold clusters via its nitrogen atom of the thiazole ring with binding energies of ca. −22 to −28 kcal mol−1 in vacuum and ca. −18 to −24 kcal mol−1 in aqueous solution. In addition to such Au–N covalent bonding, the metal–drug interactions are further stabilized by electrostatic effects, namely hydrogen-bond NH⋯Au contributions. Surface-enhanced Raman scattering (SERS) of PPX adsorbed on the Au surfaces and its desorption process were also examined. In comparison to Au8, both Au6 and Au20 clusters undergo a shorter recovery time and a larger change of energy gap, being possibly conducive to electrical conversion, thus signaling for detection of the drug. A chemical enhancement mechanism for SERS procedure was again established in view of the formation of nonconventional hydrogen interactions Au⋯H–N. The binding of PPX to a gold cluster is expected to be reversible and triggered by the presence of cysteine residues in protein matrices or lower-shifted alteration of environment pH. These findings would encourage either further theoretical probes to reach more accurate views on the efficiency of pramipexole–Au interactions, or experimental attempts to build appropriate gold nanostructures for practical trials, harnessing their potentiality for applications.Gold clusters as prospective carriers and detectors of pramipexole. 相似文献
97.
Nguyen Minh Tam Minh Quan Pham Nguyen Xuan Ha Pham Cam Nam Huong Thi Thu Phung 《RSC advances》2021,11(28):17478
The coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread worldwide recently, leading to global social and economic disruption. Although the emergently approved vaccine programs against SARS-CoV-2 have been rolled out globally, the number of COVID-19 daily cases and deaths has remained significantly high. Here, we attempt to computationally screen for possible medications for COVID-19 via rapidly estimating the highly potential inhibitors from an FDA-approved drug database against the main protease (Mpro) of SARS-CoV-2. The approach combined molecular docking and fast pulling of ligand (FPL) simulations that were demonstrated to be accurate and suitable for quick prediction of SARS-CoV-2 Mpro inhibitors. The results suggested that twenty-seven compounds were capable of strongly associating with SARS-CoV-2 Mpro. Among them, the seven top leads are daclatasvir, teniposide, etoposide, levoleucovorin, naldemedine, cabozantinib, and irinotecan. The potential application of these drugs in COVID-19 therapy has thus been discussed.Approved drugs predicted to interact with critical residues in the substrate-binding site of SARS-CoV-2 Mpro can be promising inhibitors. 相似文献
98.
Thi Le Anh Nguyen Thi Hoai Nam Doan Dinh Hieu Truong Nguyen Thi Ai Nhung Duong Tuan Quang Dorra Khiri Sonia Taamalli Florent Louis Abderrahman El Bakali Duy Quang Dao 《RSC advances》2021,11(35):21433
Antioxidant and UV absorption activities of three aaptamine derivatives including piperidine[3,2-b]demethyl(oxy)aaptamine (C1), 9-amino-2-ethoxy-8-methoxy-3H-benzo[de][1,6]naphthyridine-3-one (C2), and 2-(sec-butyl)-7,8-dimethoxybenzo[de]imidazo[4,5,1-ij][1,6]-naphthyridin-10(9H)-one (C3) were theoretically studied by density functional theory (DFT). Direct antioxidant activities of C1–C3 were firstly evaluated via their intrinsic thermochemical properties and the radical scavenging activity of the potential antioxidants with the HOO˙/HO˙ radicals via four mechanisms, including: hydrogen atom transfer (HAT), single electron transfer (SET), proton loss (PL) and radical adduct formation (RAF). Kinetic calculation reveals that HOO˙ scavenging in water occurs via HAT mechanism with C1 (kapp, 7.13 × 106 M−1 s−1) while RAF is more dominant with C2 (kapp, 1.40 × 105 M−1 s−1) and C3 (kapp, 2.90 × 105 M−1 s−1). Antioxidant activity of aaptamine derivatives can be classified as C1 > C3 > C2. Indirect antioxidant properties based on Cu(i) and Cu(ii) ions chelating activity were also investigated in aqueous phase. All three studied compounds show spontaneous and favorable Cu(i) ion chelating activity with ΔG0 being −15.4, −13.7, and −15.7 kcal mol−1, whereas ΔG0 for Cu(ii) chelation are −10.4, −10.8, and −2.2 kcal mol−1 for C1, C2 and C3, respectively. In addition, all compounds show UVA and UVB absorption; in which the excitations are determined mostly as π–π* transition. Overall, the results suggest the potential applications of the aaptamines in pharmaceutics and cosmetics, i.e. as a sunscreen and antioxidant ingredient.Antioxidant and UV absorption activities of three aaptamine derivatives were theoretically studied by density functional theory (DFT) and time-dependent density functional theory (TD-DFT). 相似文献
99.
100.
In this study, Mn2O3/MIL-100(Fe) composite was successfully synthesized by the hydrothermal method and applied for photocatalytic removal of rhodamine B (RhB) in water. The physical and chemical properties of the synthesized materials were characterized by XRD, FTIR, SEM, UV-visible, and BET analyses. Experimental results showed a great enhancement in the photocatalytic ability of the Mn2O3/MIL-100(Fe) composite as compared to individual Mn2O3 or MIL-100(Fe) under visible light and persulfate activation. The affecting factors such as pH, photocatalyst dose, RhB concentration, and Na2S2O8 concentration were investigated to find out the best conditions for efficient photocatalysis. By conducting a radical quenching test, all radicals of HO˙, SO4˙−, 1O2, and O2˙− were found to be important in photocatalytic decomposition. The mechanism was proposed for the enhancement of photocatalytic RhB removal via band potential calculation, charge separation, surface redox reaction, and key reactive oxidation species. With its durability, reusability, and high efficiency, the Mn2O3/MIL-100(Fe) composite emerges as a potential photocatalyst working under visible light for application in wastewater treatment.Effective charge transfer enhances the persulfate activation of Mn2O3/MIL-100(Fe) for photocatalytic removal of RhB under LED-generated visible light. 相似文献