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81.
Molecular cloning and sequence analysis of antigen gene tdpA of Treponema denticola. 总被引:6,自引:4,他引:6
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M Miyamoto S Noji S Kokeguchi K Kato H Kurihara Y Murayama S Taniguchi 《Infection and immunity》1991,59(6):1941-1947
We isolated and characterized an immunogenic protein of an oral spirochete, Treponema denticola Johnson. A genomic DNA library constructed with bacteriophage lambda EMBL3 as a vector was immunologically screened with a rabbit antiserum against the whole cells. Using Western immunoblot analysis, we found a particular clone encoding an antigen with a molecular weight of 53,000; we designated the antigen as T. denticola protein A (TdpA). Complete sequence determination revealed an open reading frame of 1,419 bp and a signal peptide sequence that was homologous to that of bacterial lipoprotein. Southern hybridization analysis revealed that the tdpA gene is highly conserved in six tested strains of T. denticola species. Furthermore, we found that sera from some periodontitis patients contained antibody against the TdpA protein, although the reactivities of the antibodies varied among individuals. 相似文献
82.
T Urasawa K Taniguchi N Kobayashi F Wakasugi I Oishi Y Minekawa M Oseto M U Ahmed S Urasawa 《Journal of clinical microbiology》1990,28(12):2837-2841
In our previous study (S. Urasawa, T. Urasawa, K. Taniguchi, F. Wakasugi, N. Kobayashi, S. Chiba, N. Sakurada, S. Morita, O. Morita, M. Tokieda, T. Kawamoto, K. Minekawa, and M. Oseto, J. Infect. Dis. 160:44-51, 1989) of antigenic characterization of about 300 human rotavirus (HRV) isolates collected at different localities in Japan, we found 4 HRV isolates having unique antigenic and genetic constructions. The four strains possessed both subgroup I and subgroup II antigens, serotype 3 antigen, and a long RNA electropherotype. The reactivity pattern of these four HRV isolates with three monoclonal antibodies (MAbs) directed to an outer capsid protein, VP4, and with one MAb directed to an inner capsid protein, VP2, was clearly different from those of usual subgroup II HRVs having serotype 1, serotype 3, or serotype 4 specificity and a long RNA pattern, whereas their reactivity pattern was similar to that of strain K8 (subgroup II, serotype 1), which possessed unique VP4 and VP2 proteins. RNA-RNA cross-hybridization analysis indicated that while the four isolates were genetically distinct from the two genetic groups of HRV reported previously, i.e., the Wa family (strains KU, S3, and YO) and the DS-1 family (strain S2), they were closely related to strain K8, a strain having unique antigenic and genetic properties (K. Taniguchi, K. Nishikawa, T. Urasawa, S. Urasawa, K. Midthun, A. Z. Kapikian, and M. Gorziglia, J. Virol. 63:4101-4106, 1989). 相似文献
83.
R. Tateishi K. Taniguchi T. Horai T. Iwanaga H. Taniguchi T. Kabuto M. Sano S. Ishiguro A. Wada 《Virchows Archiv : an international journal of pathology》1976,371(4):283-294
Summary In a series of 79 cases of primary esophageal carcinoma resected at The Center for Adult Diseases, Osaka, there were six tumors with specific histopathologic features valid for the diagnosis of argyrophil cell carcinoma. Of the 6 tumors, 3 were studied electron microscopically and assay for ACTH content was performed on 4 tumors.Clinically, the ages of the 6 patients ranged from 56 to 71 years; two were women and four men. Four of the 6 patients died with widespread tumor recurrences within 9 months of operation.Microscopically, the 6 tumors were composed largely or almost entirely of small, spindle-shaped cells resembling those of oat cell carcinoma of the lung, and were characterized by the arrangement of tumor cells in solid sheets or anastomosing cords, the presence of argyrophil tumor cells, and the deposits of amyloid. Electron microscopically, the three tumors contained neurosecretory-type granules. Using bioassay or radioimmunoassay ACTH activity in the tumor tissues was detected in 3 out of the 4 tumors determined.From the light and electron microscopic characteristics and the assay evidence, it seems reasonable to conclude that the 6 tumors are endocrine polypeptide producing tumors (apudomas) that arise from argyrophil cells normally found among the basal cells of the esophageal mucosa, and that they represent a distinct histopathologic entity clearly distinguishable from other types of esophageal carcinomas.Supported in part by a Grant-in Aid for Cancer Research from the Ministry of Education, Science and Culture, and the Ministry of Health and Welfare, JapanThe authors are grateful to Prof. H. Imura and Dr. Y. Hirate, Department of Internal Medicine, Kobe University School of Medicine for their interest and performing the assays for ACTH on the tumor tissues. 相似文献
84.
Emiko Ono Yuji Maeda Hidenori Tanimoto Yuma Fukutomi Chiyako Oshikata Kiyoshi Sekiya Takahiro Tuburai Naomi Turikisawa Mamoru Otomo Masami Taniguchi Houta Ishii Akihiko Asahina Eishi Miyazaki Tosihide Kumamoto Kazuo Akiyama 《Arerugī》2007,56(6):587-592
BACKGROUND: Recently, it is recognized that the patients of oral allergy syndrome (OAS) to fruits are increasing. However, there are little knowledges of the background, character, and seriousness about these patients in Kanto regions. OBJECTIVE: We aimed to investigate the clinical features of OAS patients to plant origin foods in Kanto regions. METHODS: The patient, who visited Sagamihara National Hospital from 2000 to 2005 and developed some allergic symptoms to plant origin foods, were studied by a questionary survey. RESULTS: As for the 42 subjects, average age are 36 years old, male:female=8:34, merger of other allergic disease is 35 allergic rhinitis of 42 subjects (83%), 34 of asthma (81%), 14 of atopic dermatitis (33%). The causes of OAS symptoms are 32 rose-family fruits, 34 non-rose family fruits, 14 vegetables, 11 nuts, 2 grains subjects. As for the symptom, only in the oral and pharynx symptoms are found in 12, the systemic symptoms is 29, anaphylaxis is 11 subjects. Allergic rhinitis preceded on the 90% subjects with pollinosis, very high rate. On the other hand, the 20% of all subjects have no symptoms of allergic rhinitis. CONCLUSION: A nasal catarrh symptoms went ahead in most of the OAS subjects in Kanto regions. In addition, considering from some patients have no black alder pollinosis and/or are allergic to many non-rose-family fruits at high frequency, there might be a broad cross-reactivity between many pollens other than alder and plant origin foods. 相似文献
85.
H. Ohno PhD F. Hirata K. Terayama T. Kawarabayashi R. Doi T. Kondo N. Taniguchi 《European journal of applied physiology》1983,51(2):257-268
Summary Studies of the influences of physical exercise of short duration (bicycle ergometer, 200 W for 30 min) on the activities of carbonic anhydrase isoenzymes (CA-B and CA-C types) and zinc concentration in erythrocytes were made on 5 untrained healthy male volunteers. The subjects were rested for 30 min after the exercise. There were significant decreases in the levels of zinc, CA-B, total carbonic anhydrase activity and CA-B-dependent activity immediately after exercise, but after 30 min of rest they all returned to their pre-exercise levels. No significant change in CA-C level or CA-C-dependent activity was found after exercise. Immediately after exercise, total carbonic anhydrase activity and CA-B-dependent activity following the addition of Zn2+ showed significant increases, compared with their respective activities without Zn2+ addition. However, no such effects were observed just before exercise or after rest; the addition of Zn2+ had no effect on CA-C-dependent activity at any time. A significant correlation was found between the changes in concentration of zinc and CA-B-dependent activity after exercise (r=0.711). The findings of the present study suggest that active CA-B enzymes are converted in part to inactive enzymes during acute physical exercise, possibly by decreased zinc binding. Moreover, the change in CA-B-dependent activity correlated well with the changes in pH and HCO3 concentrations in venous blood (r=0.853 and r=0.718, respectively). One may speculate that an adaptive decrease in CA-B-dependent activity in erythrocytes occurs with increased acidification in blood during heavy physical exercise of short duration.The present study was presented to the Fifth International Symposium on the Biochemistry of Exercise, Boston, June 1–5, 1982 相似文献
86.
Shigesaburo Miyakoshi Eiji Kusumi Tomoko Matsumura Akiko Hori Naoko Murashige Tamae Hamaki Koichiro Yuji Naoyuki Uchida Kazuhiro Masuoka Atsushi Wake Yoshinobu Kanda Masahiro Kami Yuji Tanaka Shuichi Taniguchi 《Biology of blood and marrow transplantation》2007,13(7):771-777
Invasive fungal infection (IFI) is a significant complication after allogeneic hematopoietic stem cell transplantation (HSCT); however, we have little information on its clinical features after reduced intensity cord blood transplantation (RICBT) for adults. We reviewed medical records of 128 patients who underwent RICBT at Toranomon Hospital between March 2002 and November 2005. Most of the patients received purine-analogbased preparative regimens. Graft-versus-host disease (GVHD) prophylaxis was a continuous infusion of either tacrolimus 0.03 mg/kg or cyclosporine 3 mg/kg. IFI was diagnosed according to the established EORTC/NIH-MSG criteria. IFI was diagnosed in 14 patients. Thirteen of the 14 had probable invasive pulmonary aspergillosis and the other had fungemia resulting from Trichosporon spp. Median onset of IFI was day 20 (range: 1-82), and no patients developed IFI after day 100. Three-year cumulative incidence of IA was 10.2%. Four of the 13 patients with invasive aspergillosis (IA) developed grade II-IV acute GVHD, and their IA was diagnosed before the onset of acute GVHD. The mortality rate of IFI was 86%. Multivariate analysis revealed that the use of prednisolone >0.2 mg/kg (relative risk 7.97, 95% confidence interval 2.24-28.4, P = .0014) was a significant risk factor for IA. This study suggests that IFI is an important cause of deaths after RICBT, and effective strategies are warranted to prevent IFI. 相似文献
87.
Miwa Tashiro Masayo Oumi Torao Yamamoto Misako Taniguchi 《Medical Electron Microscopy》1998,31(4):193-199
The intestinal absorption of a medium-chain triglyceride (MCT) was studied by electron microscopy and biochemical analysis. In jejunal absorptive cells of rats fed tricaprylin, the smooth endoplasmic reticulum in the apical cytoplasm appeared to increase in number and contained one or two particles about 40–80 nm in diameter that were less electron dense and similar in size and profile to very low density lipoprotein. Similar particles were also observed packed in the dilated Golgi sacs and in the extended intercellular spaces. These particles were remarkably increased in number as compared with those in fasted rats. Biochemical analysis of lymph from the main intestinal lymph duct showed that caprylate was apparently demonstrated only in the lymph of rats given tricaprylin at the maximum rate 3h after oral administration. The study strongly suggests that medium-chain triglyceride is at least in part transported via lacteal, possibly in the form of very low density lipoprotein. 相似文献
88.
Hirokazu Taniguchi Kenjiro Hamamoto Takahiro Nagano Yuji Kishimoto Takashi Kimura Hideki Fujitake Kojiro Yasunaga 《Medical Electron Microscopy》1993,26(1):13-18
Ultrastructural myeloperoxidase (MPO) activity and myeloid-associated antigen (MyAg) expression were investigated in 12 adult
patients with acute lymphoblastic leukemia (ALL). Ultrastructural MPO was detected by 3 different methods. Immunophenotyping
was performed by flow cytometry, using a series of monoclonal antibodies. Ultrastructural MPO-positive blast cells were detected
in 6 patients (50%). In 5 of these 6 patients, the methods detecting both MPO and platelet peroxidase (PPO) activities found
MPO-positive blast cells more frequently than those detecting MPO activity alone. In 2 patients (17%), at least one kind of
MyAg was positive. Ultrastructural MPO activity was detected more frequently than MyAg expression in ALL patients. This method
of detecting PPO and MPO is recommended for detection of ultrastructural MPO-positive ALL. 相似文献
89.
Toshikatsu Shimizu Koji Yoshitomi Junichi Taniguchi Masashi Imai 《Pflügers Archiv : European journal of physiology》1989,414(5):500-508
Morphological studies have demonstrated that a chronic increase in distal Na+ delivery causes hypertrophy of the distal convoluted tubule (DCT). To examine whether high NaCl-intake also causes functional changes in the well defined DCT, we measured transmural voltage (V
T), lumen-to-bath Na+ flux (J
Na(LB)), and net K+ secretion (J
K(net)) in DCTs obtained from control rabbits and those on high NaCl-intake diets. The lumen negativeV
T was significantly greater in the high NaCl group than in the control group. The net K+ secretion (pmol mm–1 min–1) was greater in the high NaCl-intake group (54.1±13.0 vs 14.7±5.6). The K+ permeabïlities in both luminal and basolateral DCT membranes, as assessed by the K+-induced transepithelial voltage deflection inhibitable with Ba2+, were increased in the experimental group. The lumen-to-bath22Na flux (pmol mm–1 min–1) was also greater in the experimental group (726±119 vs 396±65). TheV
T component inhibitable with amiloride was also elevated in the high NaCl-intake group. Furthermore, Na+–K+-ATPase activity of the DCT was higher in the experimental than in the control group. We conclude that high NaCl intake increases both Na+ reabsorption and K+ secretion by the DCT. This phenomenon is associated with an increased Na+–K+-ATPase activity along with increased Na+ and K+ permeabilities of the luminal membrane, and an increase in the K+ permeability of the basolateral membrane. Cellular mechanisms underlying these functional changes remain to be established. 相似文献
90.
A. N. Chalazonitis MD Ph.D D. Koumarianos RT J. Tzovara MD P. Chronopoulos MD 《Journal of digital imaging》2003,16(2):216-229
Over the past decade, the technology that permits images to be digitized and the reduction in the cost of digital equipment allows quick digital transfer of any conventional radiological film. Images then can be transferred to a personal computer, and several software programs are available that can manipulate their digital appearance. In this article, the fundamentals of digital imaging are discussed, as well as the wide variety of optional adjustments that the Adobe Photoshop 6.0 (Adobe Systems, San Jose, CA) program can offer to present radiological images with satisfactory digital imaging quality. 相似文献