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101.
Strain variation among Bordetella pertussis isolates from Québec and Alberta provinces of Canada from 1985 to 1994 下载免费PDF全文
Peppler MS Kuny S Nevesinjac A Rogers C de Moissac YR Knowles K Lorange M De Serres G Talbot J 《Journal of clinical microbiology》2003,41(7):3344-3347
Pulsed-field gel electrophoresis and gene typing were able to differentiate among 3,597 Bordetella pertussis isolates circulating in Alberta and Québec Provinces, Canada, from 1985 to 1994 and distinguish them from the strains used in vaccine production. This study provides a baseline for continued surveillance of prevalent and emerging strains of B. pertussis in Canada. 相似文献
102.
Balanos GM Talbot NP Robbins PA Dorrington KL 《Pflügers Archiv : European journal of physiology》2005,450(6):372-380
In healthy humans, changes in cardiac output are commonly accommodated with minimal change in pulmonary artery pressure. Conversely, exposure to hypoxia is associated with substantial increases in pulmonary artery pressure. In this study we used non-invasive measurement of an index of pulmonary artery pressure, the maximum systolic pressure difference across the tricuspid valve (Pmax), to examine the pulmonary vascular response to changes in blood flow during both air breathing and hypoxia. We used Doppler echocardiography in 33 resting healthy humans breathing air over 6–24 h to measure spontaneous diurnal variations in Pmax and cardiac output. Cardiac output varied by up to ~2.5 l/min; Pmax varied little with cardiac output [0.61±0.74 (SD) mmHg min l–1]. Eight of the volunteers were also exposed to eucapnic hypoxia (end-tidal
) for 8 h. In this group Pmax rose progressively from 21 mmHg to 37 mmHg over 8 h. By comparing diurnal variations in Pmax during air breathing with changes in Pmax during hypoxia in the same eight individuals, we concluded that only approximately 5% of the changes in Pmax during hypoxia could be attributed to concurrent changes in cardiac output. The low sensitivity of Pmax to changes in cardiac output makes it a useful index of hypoxic pulmonary vasoconstriction in healthy humans. 相似文献
103.
Mathilde W. N. Yu Suzanne Lemieux Pierre J. Talbot 《European journal of immunology》1996,26(12):3230-3233
The idiotypic network can be experimentally altered to induce protective immune responses against microbial pathogens. Both internal image and noninternal image anti-idiotypic (anti-Id) antibodies have been shown to trigger antigen (Ag)-specific immune responses. Therefore, mechanisms of anti-Id vaccination appear to go beyond structural mimicry of Ag, but remain undefined. Using the neurotropic murine coronavirus animal model, we have previously shown that a polyclonal noninternal image anti-Id (Ab2) could vaccinate BALB/c mice. To characterize its mode of action, we have examined the immune modulating capability of this Ab2 in vivo in strains of mice with different H-2 haplotypes. Even though only internal image anti-Id are expected to induce non-genetically restricted immunity, this noninternal image Ab2 induced protective immunity in four of eight genetically different strains of mice susceptible to coronavirus infection. These were BALB/c (H-2d), DBA/1 (H-2d), DBA/1 (H-2q), and SWR (H-2q) mice. Protection was generally correlated with the induction of specific antiviral Ab (Ab3) that showed biological properties, such as virus neutralization in vitro, similar to the initial Ab1. To evaluate the genetic implication of the H-2 haplotypes in protection, congenic mice were also tested. Vaccination profiles suggest that cooperation between background gene(s) of the BALB/c mouse with H-2d and H-2q loci is necessary for an optimal protective immune response, although the main genetic element(s) regulating the antiviral response to Ab2 inoculation appeared to be located outside the major histocompatibility complex. These results are consistent with the ability of Ab2 to induce protective antiviral antibodies in genetically different animals by biological mimicry. 相似文献
104.
Summary Electron microscopy of toad (Bufo marinus) muscle fixed without relaxing after a single eccentric contraction at muscle lengths greater than optimum showed over-stretched half-sarcomeres in sufficient numbers to account for more than half of the imposed stretch. Such sarcomeres were absent in another muscle fixed without relaxing after an isometric contraction at the same length and largely absent in a third muscle that underwent eccentric contraction at muscle lengths less than optimum. This provides direct evidence in support of the hypothesis that lengthening of muscles at long length involves lengthening of a few half sarcomeres to beyond filament overlap, while most half sarcomeres are extended much less than in proportion to muscle extension. 相似文献
105.
The CTLA-4 gene region of chromosome 2q33 is linked to, and associated with, type 1 diabetes. Belgian Diabetes Registry 总被引:8,自引:1,他引:8
Nistico L; Buzzetti R; Pritchard LE; Van der Auwera B; Giovannini C; Bosi E; Larrad MT; Rios MS; Chow CC; Cockram CS; Jacobs K; Mijovic C; Bain SC; Barnett AH; Vandewalle CL; Schuit F; Gorus FK; Tosi R; Pozzilli P; Todd JA 《Human molecular genetics》1996,5(7):1075-1080
Susceptibility to autoimmune insulin-dependent (type 1) diabetes mellitus
is determined by a combination of environmental and genetic factors, which
include variation in MHC genes on chromosome 6p21 (IDDM1) and the insulin
gene on chromosome 11p15 (IDDM2). However, linkage to IDDM1 and IDDM2
cannot explain the clustering of type 1 diabetes in families, and a role
for other genes is inferred. In the present report we describe linkage and
association of type 1 diabetes to the CTLA-4 gene (cytotoxic T lymphocyte
associated-4) on chromosome 2q33 (designated IDDM12). CTLA-4 is a strong
candidate gene for T cell- mediated autoimmune disease because it encodes a
T cell receptor that mediates T cell apoptosis and is a vital negative
regulator of T cell activation. In addition, we provide supporting evidence
that CTLA-4 is associated with susceptibility to Graves' disease, another
organ- specific autoimmune disease.
相似文献
106.
A genetic linkage map for zebrafish: comparative analysis and localization of genes and expressed sequences 下载免费PDF全文
Gates MA Kim L Egan ES Cardozo T Sirotkin HI Dougan ST Lashkari D Abagyan R Schier AF Talbot WS 《Genome research》1999,9(4):334-347
Genetic screens in zebrafish (Danio rerio) have isolated mutations in hundreds of genes with essential functions. To facilitate the identification of candidate genes for these mutations, we have genetically mapped 104 genes and expressed sequence tags by scoring single-strand conformational polymorphisms in a panel of haploid siblings. To integrate this map with existing genetic maps, we also scored 275 previously mapped genes, microsatellites, and sequence-tagged sites in the same haploid panel. Systematic phylogenetic analysis defined likely mammalian orthologs of mapped zebrafish genes, and comparison of map positions in zebrafish and mammals identified significant conservation of synteny. This comparative analysis also identified pairs of zebrafish genes that appear to be orthologous to single mammalian genes, suggesting that these genes arose in a genome duplication that occurred in the teleost lineage after the divergence of fish and mammal ancestors. This comparative map analysis will be useful in predicting the locations of zebrafish genes from mammalian gene maps and in understanding the evolution of the vertebrate genome. 相似文献
107.
108.
Alexandra Dimitrakopoulou Ernest Schilders Quamar Bismil J. Charles Talbot Konstantinos Kazakos 《Knee surgery, sports traumatology, arthroscopy》2010,18(5):691-693
We present a case of a high-level rugby player with severe groin pain following a partial rupture of his left adductor longus enthesis during a game. Conservative treatment proved unsuccessful and the athlete had persistent symptoms, affecting his quality of life and ability to play sports. Further assessments revealed a large bony spur/enthesophyte at adductor longus origin. The patient underwent a successful surgical resection of the active bone formation. 相似文献
109.
H. Keipp B. Talbot James E. Crowe Jr. Kathryn M. Edwards Marie R. Griffin Yuwei Zhu Geoffrey A. Weinberg Peter G. Szilagyi Caroline B. Hall Amy B Podsiad Marika Iwane John V. Williams 《Journal of medical virology》2009,81(5):853-856
There is only limited knowledge on the burden of disease due to both new (HCoV‐NL63 and HKU‐1) and previously discovered coronaviruses (OC43 and 229E) in children. Respiratory specimens and clinical data were prospectively collected in an active, population‐based surveillance study over a 2‐year period from children aged <5 years hospitalized with acute respiratory symptoms or fever. These samples were retrospectively tested by real‐time RT‐PCR for HCoV‐NL63, HKU1, OC43, and 229E. Human coronaviruses (HCoVs) were identified in 2.2% of study children <2 years of age. Rates of HCoV‐associated hospitalization per 10,000 were 10.2 (95% CI 4.3, 17.6), 4.2 (95% CI 1.9, 6.9), and 0 (95% CI 0, 3.7) in children aged <6 months, 6–23 months, and 24–59 months, respectively. Coronaviruses were identified in a modest number of hospitalized children. J. Med. Virol. 81:853–856, 2009. © 2009 Wiley‐Liss, Inc. 相似文献