全文获取类型
收费全文 | 14487篇 |
免费 | 669篇 |
国内免费 | 90篇 |
专业分类
耳鼻咽喉 | 219篇 |
儿科学 | 347篇 |
妇产科学 | 188篇 |
基础医学 | 2019篇 |
口腔科学 | 255篇 |
临床医学 | 892篇 |
内科学 | 3804篇 |
皮肤病学 | 268篇 |
神经病学 | 1017篇 |
特种医学 | 485篇 |
外科学 | 2692篇 |
综合类 | 33篇 |
预防医学 | 293篇 |
眼科学 | 173篇 |
药学 | 936篇 |
中国医学 | 42篇 |
肿瘤学 | 1583篇 |
出版年
2023年 | 81篇 |
2022年 | 134篇 |
2021年 | 295篇 |
2020年 | 157篇 |
2019年 | 191篇 |
2018年 | 270篇 |
2017年 | 213篇 |
2016年 | 269篇 |
2015年 | 271篇 |
2014年 | 366篇 |
2013年 | 468篇 |
2012年 | 723篇 |
2011年 | 879篇 |
2010年 | 493篇 |
2009年 | 385篇 |
2008年 | 693篇 |
2007年 | 807篇 |
2006年 | 769篇 |
2005年 | 849篇 |
2004年 | 869篇 |
2003年 | 794篇 |
2002年 | 871篇 |
2001年 | 378篇 |
2000年 | 362篇 |
1999年 | 315篇 |
1998年 | 211篇 |
1997年 | 201篇 |
1996年 | 161篇 |
1995年 | 123篇 |
1994年 | 151篇 |
1993年 | 146篇 |
1992年 | 220篇 |
1991年 | 226篇 |
1990年 | 196篇 |
1989年 | 206篇 |
1988年 | 159篇 |
1987年 | 169篇 |
1986年 | 182篇 |
1985年 | 153篇 |
1984年 | 102篇 |
1983年 | 84篇 |
1982年 | 64篇 |
1981年 | 41篇 |
1980年 | 48篇 |
1979年 | 47篇 |
1978年 | 52篇 |
1977年 | 37篇 |
1976年 | 34篇 |
1970年 | 34篇 |
1968年 | 34篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
102.
Tamiya G Shinya M Imanishi T Ikuta T Makino S Okamoto K Furugaki K Matsumoto T Mano S Ando S Nozaki Y Yukawa W Nakashige R Yamaguchi D Ishibashi H Yonekura M Nakami Y Takayama S Endo T Saruwatari T Yagura M Yoshikawa Y Fujimoto K Oka A Chiku S Linsen SE Giphart MJ Kulski JK Fukazawa T Hashimoto H Kimura M Hoshina Y Suzuki Y Hotta T Mochida J Minezaki T Komai K Shiozawa S Taniguchi A Yamanaka H Kamatani N Gojobori T Bahram S Inoko H 《Human molecular genetics》2005,14(16):2305-2321
A major goal of current human genome-wide studies is to identify the genetic basis of complex disorders. However, the availability of an unbiased, reliable, cost efficient and comprehensive methodology to analyze the entire genome for complex disease association is still largely lacking or problematic. Therefore, we have developed a practical and efficient strategy for whole genome association studies of complex diseases by charting the human genome at 100 kb intervals using a collection of 27,039 microsatellites and the DNA pooling method in three successive genomic screens of independent case-control populations. The final step in our methodology consists of fine mapping of the candidate susceptible DNA regions by single nucleotide polymorphisms (SNPs) analysis. This approach was validated upon application to rheumatoid arthritis, a destructive joint disease affecting up to 1% of the population. A total of 47 candidate regions were identified. The top seven loci, withstanding the most stringent statistical tests, were dissected down to individual genes and/or SNPs on four chromosomes, including the previously known 6p21.3-encoded Major Histocompatibility Complex gene, HLA-DRB1. Hence, microsatellite-based genome-wide association analysis complemented by end stage SNP typing provides a new tool for genetic dissection of multifactorial pathologies including common diseases. 相似文献
103.
104.
The distinction between Burkitt lymphoma and diffuse large B-Cell lymphoma with c-myc rearrangement. 总被引:2,自引:0,他引:2
Naoya Nakamura Hirokazu Nakamine Jun-Ichi Tamaru Shigeo Nakamura Tadashi Yoshino Kouichi Ohshima Masafumi Abe 《Modern pathology》2002,15(7):771-776
To compare immunophenotypic and molecular features between Burkitt lymphoma (BL) and diffuse large B-cell lymphoma (DLBCL) with c-myc rearrangements (c-mycR DLBCL), we analyzed 18 cases of B-cell non-Hodgkin's lymphoma with c-mycR that were confirmed by chromosomal and/or Southern blotting analyses. The cases were histologically classified into 10 BLs and five DLBCLs. The remaining three cases could not be classified because of suboptimal quality of the surgical materials. BLs were from five adults and five children, whereas all DLBCLs were from adults. BLs were positive for CD20 (10/10 cases examined), CD10 (9/10), Bcl-2 (1/9), and Bcl-6 (10/10), whereas they were negative for CD3 (0/10) and EBV (0/8), by Epstein-Barr virus (EBV) EBER-1 RNA in situ hybridization. c-MycR DLBCLs were positive for CD20 (5/5), CD10 (2/5), Bcl-2 (3/4), and Bcl-6 (4/4), whereas none of them were positive for CD3 and EBV. A mean of MIB-1 index (MIB-1+ cells/neoplastic cells, %) of BLs (98.1%) was higher than that of c-mycR DLBCLs (66.3%; P <.0001). Somatic mutation of immunoglobulin heavy-chain gene variable region (VH gene) in BLs (four cases) ranged from 0.7 to 4.9% with an average value of 2.3%, whereas those in DLBCLs (three cases) from 8.2 to 32.0% with an average value of 17.0%. It is, therefore, concluded that a growth fraction of nearly 100%, as well as a monotonous proliferation of medium-sized cells and c-myc(R), should be of value in the diagnosis of BL, which is probably different from c-myc(R) DLBCL. In addition, CD10+, Bcl-2-, and low frequency of mutation of the VH gene could be helpful for the histologic distinction of BL from (c-mycR) DLBCL. 相似文献
105.
Yasuyuki Shibuya Hisashi Yasuda Masaki Tomatsuri Akira Mizoguchi Masatoshi Takeichi Keikichi Shimada Chizuka Ide 《Journal of neurocytology》1996,25(1):615-624
Summary The Ca2+-dependent intercellular adhesion molecule cadherin is known to be linked to the cytoskeleton by the protein catenin, an association of which appears to be important for the cell-adhesion function of cadherin. Catenin consists of three subtypes-, , and . In our previous study, N-cadherin was shown to be localized on the plasmalemma of normal and regenerating chick peripheral nerve. Thus, as N-catenin is a subtype of -catenin (which is specifically associated with N-cadherin), we investigated the immunolocalization of N-catenin in normal and regenerating chick sciatic nerve. In normal nerve, unmyelinated axons exhibited either intense or weak N-catenin immunoreactivity throughout the axoplasm, whereas myelinated axons were completely immunonegative. Regenerating axons, including those derived from parent myelinated axons, showed N-catenin immunoreactivity of variable intensities in growth cones and axon shafts. Schwann cells were invariably devoid of immunoreactivity. Thus N-catenin is not necessarily bound to the surface plasmalemma, but is distributed throughout the cytoplasm, suggesting that most N-catenin molecules are dissociated from N-cadherin. 相似文献
106.
Shin Nishimura Akio Takahashi Tadashi Narita Tokio Hagiwara Hiroshi Hamana 《Macromolecular chemistry and physics.》1995,196(6):1953-1961
Initiation and propagation reaction mechanisms of the anionic polymerization of hexafluoro-1,3-butadiene (HEBD) were investigated. The initiation reaction with caesium tert-butoxide was found to be completed within 5 min although the reactions were carried out at a much lower temperature than that of the polymerization reaction. The initiation reaction was, therefore, inferred to take place in an anionic fashion by adding the tert-butoxide anion to HFBD. In order to clarify the propagation reaction mechanism of HFBD which yielded a polymer with a polyvinylene structure, the polymerization reactivity of HFBD and hexafluoro-2-butyne (HFBY), the isomerization of HFBD to HFBY, and the structural difference between poly(HFBD) and poly(HFBY) were discussed. In spite of the low yield of HFBY by the isomerization reaction under polymerization conditions, higher yields of poly(HFBD) were obtained. Judging from the X-ray analysis which showed that poly(HFBD) was highly crystalline and poly(HFBY) was amorphous, poly(HFBD) might not be produced by polymerization of HFBY. An addition reaction of the propagating anion to the carbon-2 of the HFBD monomer followed by isomerization at the propagating living end to yield poly[1,2-bis(trifluoromethyl)vinylene] is proposed. 相似文献
107.
Nakajima T Yasuda T Takeshita H Nakashima Y Mori S Mogi K Kishi K 《Experimental and clinical immunogenetics》2000,17(2):71-76
Five anti-human deoxyribonuclease I (DNase I) monoclonal antibodies were obtained from BALB/c mice immunized with DNase I purified from human urine. Four of them inhibited DNase I enzyme activity, as did a rabbit polyclonal antibody; these 4 did not have immunostaining ability. The remaining one had immunostaining ability but no inhibitory activity. A Sepharose 4B column conjugated with 1 of the 4 antibodies that had inhibitory activity effectively adsorbed and eluted the DNase I enzyme; this did not occur with the rabbit polyclonal antibody. We showed that adding an immunoaffinity chromatography step made the purification of human DNase I easier and faster than the conventional procedure. 相似文献
108.
Therapeutic effect of topical administration of SN50, an inhibitor of nuclear factor-kappaB, in treatment of corneal alkali burns in mice 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Saika S Miyamoto T Yamanaka O Kato T Ohnishi Y Flanders KC Ikeda K Nakajima Y Kao WW Sato M Muragaki Y Ooshima A 《The American journal of pathology》2005,166(5):1393-1403
We evaluated the therapeutic efficacy of topical administration of SN50, an inhibitor of nuclear factor-kappaB, in a corneal alkali burn model in mice. An alkali burn was produced with 1 N NaOH in the cornea of C57BL/6 mice under general anesthesia. SN50 (10 microg/microl) or vehicle was topically administered daily for up to 12 days. The eyes were processed for histological or immunohistochemical examination after bromodeoxyuridine labeling or for semi-quantification of cytokine mRNA. Topical SN50 suppressed nuclear factor-kappaB activation in local cells and reduced the incidence of epithelial defects/ulceration in healing corneas. Myofibroblast generation, macrophage invasion, activity of matrix metalloproteinases, basement membrane destruction, and expression of cytokines were all decreased in treated corneas compared with controls. To elucidate the role of tumor necrosis factor (TNF)-alpha in epithelial cell proliferation, we performed organ culture of mouse eyes with TNF-alpha, SN50, or an inhibitor of c-Jun N-terminal kinase (JNK) and examined cell proliferation in healing corneal epithelium in TNF-alpha-/- mice treated with SN50. An acceleration of epithelial cell proliferation by SN50 treatment was found to depend on TNF-alpha/JNK signaling. In conclusion, topical application of SN50 is effective in treating corneal alkali burns in mice. 相似文献
109.
Ryuei Maeda Nobuo Ihara Reiko Takada Tadashi Kondo Emyo Nakano Toshiko Yamato 《Pathology international》1971,21(1):151-155
A new vital staining method with neutral red has been established where by cerebral ganglion cells can be stained in vivo . 相似文献
110.
Mr N. Ishikawa S. Suda T. Sasaki T. Yamanishi H. Hosaka K. Yasuda H. Ito 《Medical & biological engineering & computing》1998,36(6):704-710
A system composed of a functional continuous magnetic stimulator (FCMS) and a saddle-type coil has been developed for non-invasive
treatment of urinary incontinence, especially stress incontinence and urge incontinence. The FCMS conditions were as follows:
2 kW maximum electrical power consumption, 800 V maximum capacitor voltage, 720 μs pulsewidth (180 μs rise time), and 5–30
Hz frequency. A frequency between 5 and 10 Hz is used to treat urge incontinence and a frequency between 25 Hz and 30 Hz is
used to treat urge incontinence. The coil (120 mm long, 90 mm wide and 50 mm thick) fits the most suitable region for this
treatment, the region from the anus to the perineum. The coil is cooled to maintain a coil temperature between 20 and 25°C
so that it can be used efficiently and safely. In experiments with anaesthetised dogs, it was confirmed that the urethral
pressure increased when the circumference of the perineum received continuous magnetic stimulation of 720 μs pulsewidth (180
μs rise time), 10Hz frequency and about 520 V capacitor voltage. This result suggests that magnetic stimulation can be effective
as a urinary incontinence therapy. 相似文献