Establishment of Cell Lines Derived from Squamous Cell Carcinoma of the Esophagus in Humans and Rats

Two epithelial cell lines (TH and TS) were established fromsquamous cell carcinoma of the esophagus in humans. ]n addition,three cell lines (R-1, R-2 and R-3) were established from squamouscell carcinoma of the esophagus induced in Donryu rats by N-amyl-N-Methyl-nitrosamine.TH line has been subcultured more than 300 times in 3 yr andthe TS line 216 times in 3 yr and 5 mo, while the R-1, R-2 andR-3 lines have had more than 300 passages in 2 yr and 11 mo,2 yr and 8 mo and 2 yr and 4 mo, respectively. The average doublingtime was 17 hr for TH, 47 for TS, 15 for R-1, 16 for R-2 and21 for R-3. These cells were characterized by the presence ofdesmosomes and a markedly heteroploid karyotype. The lacticdehydrogenase isozyme pattern of the TH line showed a tumorpattern. Heterotransplantation of the five cultured lines intonude mice produced tumors.     

Dimension discrimination at two different degrees of mouth opening and the effect of anaesthesia applied to the periodontal ligaments

The dimension discrimination ability of twenty-five young persons was tested at two different degrees of mouth opening. One reference stick of 10-0 mm and another of 45-0 mm was placed between the teeth, alternating with test sticks of different heights. The subjects were asked to assess whether a test stick felt higher or lower than the reference stick. The experiment was performed with and without anaesthesia applied to the periodontal ligaments. Anaesthesia did not impair the discrimination ability. At dimensions in the region of the 10-0 mm stick the incorrect assessments occurred predominantly among sticks higher than the reference stick. Around the 45-0 mm stick incorrect assessments were distributed evenly between dimensions higher or lower than the reference stick.     

Double Adenocarcinoma in Barrett's Esophagus: A Case Report

At the National Cancer Center Hospital in Tokyo, a patient withBarrett's esophagus developed double adenocarcinoma of the esophagus.One carcinoma was located in the midesophagus and the otherjust above the anatomic cardia. Esophagoscopic examination withbiopsy revealed two carcinomas surrounded with columnar epitheliumand ectopic islets of gastric mucosa situated in the postcricoidregion. There was no ulcer or stricture in the esophagus. Thepatient received subtotal esophagectomy and survived the operation.Microscopically, depth of invasion of the proximal cancer wasto the proper muscle, and that of the distal one was to thesubmucosal layer. There was metastasis to two lymph nodes. Therewas no sign of inflammation or ulcer in the esophagus.     

Slow Potential-Guided Radiofrequency Catheter Ablation in Atrioventricular Nodal Reentrant Tachycardia: Characteristics of the Potential Associated with Successful Ablation

To examine the characteristics of Haïssaguerre's slow potential (SP) specific to effective catheter ablation of the slow pathway in AV nodal reentrant tachycardia, the properties of SP and its recording site ware analyzed in 52 patients who underwent successful SP-guided ablation. The properties of SP included the ratio of the amplitude of SP to that of atrial potential (A)(SP/A), the SP duration, the interval between His-bundle potential (HP) and SP (HP-SP), the interval between A and SP (A-SP), the interval between SP and ventricular potential (V) (SP-V), and the ratio of A-SP to the interval between A and the V (A-SP/A-V). The SP recording site was determined by the ratio of the amplitude of A to that of V (A/V) and by the relative position of the ablation catheter on X ray (right anterior oblique projection), expressed as the ratio of the distance between the coronary sinus ostium and SP site to that between the coronary sinus ostium and HP recording site (relative SP position). Twenty-eight slow pathways were ablated with a single energy application, while the other 24 required applications ≥ 2. In all successful applications, SP/A, SP duration, HP-SP, A-SP. SP-V, A-SP/A-V, A/V, and relative SP position were 51 %± 25%, 28 ± 5 ms, -11 ± 9 ms, 57 ± 25 ms, 68 ± 13 ms, 46%± 9%, 15%± 13%, and 51%± 13%, respectively. A significant correlation was observed between the relative SP position and A-SP, and between the relative SP position and A-SP/A-V (r = 0.60 and 0.37, respectively), while it was not between the relative SP position and HP-SP, nor between the relative SP position and SP-V. When the characteristics of SP were comparatively analyzed between the effective and ineffective applications in 24 patients in whom applications ≥ 2 were required, there was no difference observed in HP-SP, A-SP, SP-V, A-SP/A-V, and A/V. However, SP/A, SP duration, and the relative SP position in the effective applications were all greater than those in the ineffective ones (56%± 20% vs 35%± 18%, P < 0.001; 29 ± 4 vs 26 ± 5 ms, P < 0.01; and 52%± 15% vs 33%± 11%, P < 0.001, respectively). These results indicate that SP with an amplitude over a half of A amplitude and recorded at the mid-septum of the tricuspid annulus can be a marker for successful slow pathway ablation. Although the local atrial electrogram appears late as the SP recording site shifts to the lower position, the timing of SP relative to HP and V remained unchanged, suggesting that SP is independent of the local atrial activation.     

Augmented levels of gastric mucosal leucocyte activation by infection with cagA gene-positive Helicobacter pylori

The possession of the cytotoxin-associated gene (cagA) of Helicobacter pylori is thought to be highly associated with peptic ulcer disease. However, the pathogenic role of cagA is still unknown. We have emphasized the importance of the interrelationship between H. pylori-derived ammonia and oxygen radicals from in?ltrated leucocytes. The aim of the present study was to explore the relationship between oxygen radical production and H. pylori strain diversity based on cagA possession. An endoscopic examination and gastric mucosal biopsy were performed in 30 H. pylori-infected patients with gastric ulcer. Myeloperoxidase (MPO) content and the luminol-dependent chemiluminescence value in the biopsied gastric specimens were measured as an index for leucocyte in?ltration and oxygen radical production. From the precipitates of cultured bacterial isolates of biopsied specimens, bacterial DNA was puri?ed and analysed by polymerase chain reaction to characterize the possession of cagA. While all patients had ureC-positive strains, 22 had cagA-positive and eight had cagA-negative strains. In patients with cagA-positive strains, MPO contents as well as chemiluminescence values in the gastric corpus were signi?cantly higher than those in patients with cagA-negative strains. Gastric mucosal leucocyte recruitment and activation are suggested to be enhanced by cagA gene-positive H. pylori infection.     

An optical system for measuring inclination and area of occlusal facets

summary Occlusal facets are regarded as an individual record of occlusal contacts that result from various mandibular movements. Analysing characteristics of the facets is important for the functional assessment of the stomatognathic system. A new system which quantifies such characteristics optically has been developed. This system is composed of optical devices, a graphic image-processor, a set of stages for mounting and positioning the model and laser indicators of the measuring point. The system enables the inclination and area of each facet and its location to be measured and its processed image displayed on the graphic monitor.     

Analysis of differences in clinicopathological features between prostate cancers located in the transition and peripheral zones

BACKGROUND: The objective of this study was to retrospectively characterize differences in the clinicopathological features of prostate cancer according to the zonal origin. METHODS: Among 185 consecutive patients who underwent radical prostatectomy without any neoadjuvant hormonal therapies, this study included 134 patients who were diagnosed as having either transition zone (TZ) or peripheral zone (PZ) cancer according to the following criteria: TZ or PZ cancers were considered when more than 70% of the cancer area was located in the TZ or PZ, respectively. The various clinicopathological features were then compared according to this classification. RESULTS: In this series, 27 patients were diagnosed as having TZ cancer, while the remaining 107 were diagnosed as having PZ cancer. The percent of positive biopsy cores in TZ cancers was significantly lower than that in PZ cancers; however, there were no significant differences in the anatomical location of positive cores between these two groups except for the middle of prostate where TZ cancer showed a significantly lower rate of positive biopsies than PZ cancer. The preoperative serum prostate-specific antigen (PSA) value in patients with TZ cancer was significantly higher than that in those with PZ cancer. Furthermore, tumor volume in TZ cancers was significantly greater than that in PZ cancers. However, there was no significant difference in biochemical recurrence-free survival between patients with TZ and PZ cancers. CONCLUSIONS: Despite the significantly high PSA value as well as great tumor volume compared with those of PZ cancers, TZ cancers had similar biochemical cure rates following radical prostatectomy, suggesting a less aggressive phenotype of TZ cancers than that of PZ cancers.     

A Case of Concomitant Association of Early Esophageal Carcinoma, Early Gastric Carcinoma and Malignant Lymphoma of the Stomach

A case of concomitant association of early esophageal carcinoma,cancer of the early gastric carcinoma and malignant lymphomaof the stomach was reported. The intrathoracic esophagus wasresected with the whole stomach, and the esophagus was reconstructedwith the right side colon. There were two separate early carcinomasin the upper and lower esophagus. The upper lesion was intraepithelialsquamous cell carcinoma, and the extension of lower lesion alsolimited in the intraepithelial layer. He had also signet ringcell carcinoma of the stomach which was limited only in themucosal layer. In addition, there was malignant lymphoma invadedinto the mucosa and sub-mucosa at the antral rigion of the stomach.There was no lymph node metastasis. The patient is living wellnow. The association of early esophageal carcinoma with earlystomach carcinoma is rare. Only five such cases have been reportedin the Japanese literature.