全文获取类型
收费全文 | 2663篇 |
免费 | 194篇 |
国内免费 | 9篇 |
专业分类
耳鼻咽喉 | 20篇 |
儿科学 | 56篇 |
妇产科学 | 22篇 |
基础医学 | 446篇 |
口腔科学 | 20篇 |
临床医学 | 318篇 |
内科学 | 497篇 |
皮肤病学 | 18篇 |
神经病学 | 423篇 |
特种医学 | 118篇 |
外科学 | 344篇 |
综合类 | 3篇 |
一般理论 | 5篇 |
预防医学 | 136篇 |
眼科学 | 66篇 |
药学 | 202篇 |
中国医学 | 5篇 |
肿瘤学 | 167篇 |
出版年
2024年 | 3篇 |
2023年 | 18篇 |
2022年 | 41篇 |
2021年 | 109篇 |
2020年 | 45篇 |
2019年 | 93篇 |
2018年 | 101篇 |
2017年 | 58篇 |
2016年 | 88篇 |
2015年 | 105篇 |
2014年 | 119篇 |
2013年 | 156篇 |
2012年 | 238篇 |
2011年 | 253篇 |
2010年 | 141篇 |
2009年 | 107篇 |
2008年 | 173篇 |
2007年 | 179篇 |
2006年 | 166篇 |
2005年 | 150篇 |
2004年 | 135篇 |
2003年 | 120篇 |
2002年 | 82篇 |
2001年 | 18篇 |
2000年 | 9篇 |
1999年 | 14篇 |
1998年 | 27篇 |
1997年 | 20篇 |
1996年 | 16篇 |
1995年 | 14篇 |
1994年 | 10篇 |
1993年 | 14篇 |
1992年 | 4篇 |
1991年 | 5篇 |
1990年 | 6篇 |
1989年 | 4篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1973年 | 1篇 |
1933年 | 3篇 |
1931年 | 2篇 |
排序方式: 共有2866条查询结果,搜索用时 31 毫秒
71.
72.
The capacity of blood group antibodies to agglutinate red cells suspended in saline is largely dependent on the antibody isotype. The immunological cross-linking of IgG antibodies has previously been described as a means to increase the reactivity of IgG in many situations. We have prepared anti-D-containing complexes by blending a human IgG anti-D monoclonal antibody (mAb) and a murine anti-human IgG mAb. In standard red cell serology assays, the anti-D complexes exhibited a very high avidity and could agglutinate weak D-positive red cells in direct saline testing. These results indicate that potent saline hemagglutinating reagents of RhD and eventually of other blood group specificities can be prepared from IgG mAbs. 相似文献
73.
The domain structure of protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Sylvain Arnould Jean-Michel Camadro 《Proceedings of the National Academy of Sciences of the United States of America》1998,95(18):10553-10558
Protoporphyrinogen oxidase (EC 1–3-3–4), the 60-kDa membrane-bound flavoenzyme that catalyzes the final reaction of the common branch of the heme and chlorophyll biosynthesis pathways in plants, is the molecular target of diphenyl ether-type herbicides. It is highly resistant to proteases (trypsin, endoproteinase Glu-C, or carboxypeptidases A, B, and Y), because the protein is folded into an extremely compact form. Trypsin maps of the native purified and membrane-bound yeast protoporphyrinogen oxidase show that this basic enzyme (pI > 8.5) was cleaved at a single site under nondenaturing conditions, generating two peptides with relative molecular masses of 30,000 and 35,000. The endoproteinase Glu-C also cleaved the protein into two peptides with similar masses, and there was no additional cleavage site under mild denaturing conditions. N-terminal peptide sequence analysis of the proteolytic (trypsin and endoproteinase Glu-C) peptides showed that both cleavage sites were located in putative connecting loop between the N-terminal domain (25 kDa) with the βαβ ADP-binding fold and the C-terminal domain (35 kDa), which possibly is involved in the binding of the isoalloxazine moiety of the FAD cofactor. The peptides remained strongly associated and fully active with the Km for protoporphyrinogen and the Ki for various inhibitors, diphenyl-ethers, or diphenyleneiodonium derivatives, identical to those measured for the native enzyme. However, the enzyme activity of the peptides was much more susceptible to thermal denaturation than that of the native protein. Only the C-terminal domain of protoporphyrinogen oxidase was labeled specifically in active site-directed photoaffinity-labeling experiments. Trypsin may have caused intramolecular transfer of the labeled group to reactive components of the N-terminal domain, resulting in nonspecific labeling. We suggest that the active site of protoporphyrinogen oxidase is in the C-terminal domain of the protein, at the interface between the C- and N-terminal domains. 相似文献
74.
Intra-left ventricular electromechanical asynchrony. A new independent predictor of severe cardiac events in heart failure patients 总被引:24,自引:0,他引:24
Bader H Garrigue S Lafitte S Reuter S Jaïs P Haïssaguerre M Bonnet J Clementy J Roudaut R 《Journal of the American College of Cardiology》2004,43(2):248-256
OBJECTIVES: We sought to assess the electromechanical parameters, using tissue Doppler echocardiography, as potential independent predictors of heart failure (HF) worsening. BACKGROUND: Ventricular conduction disorders worsen the prognosis for HF patients. However, the relationships between the QRS width and morphology, hemodynamic parameters, and presence and magnitude of intra-left ventricular (LV) and inter-ventricular (V) asynchrony have not been well clarified. METHOD: A total of 104 patients with an LV ejection fraction (EF) =45% and stabilized HF, without myocardial infarction (MI), underwent echocardiography coupled with tissue Doppler imaging and were followed for one year. The protocol analyzed the incidence of worsening HF (hospitalization for cardiac decompensation). Inter-V and regional electromechanical delays for the anterior, septal, inferior, and lateral LV walls were correlated with the QRS morphology and duration. The intra-LV and inter-V asynchrony values of these patients were compared with those of healthy subjects matched by gender and age criteria to determine the respective normal ranges. RESULTS: The presence of intra-LV (but not inter-V) asynchrony was identified as an independent predictor of severe cardiac events (hazard ratio 3.39, p < 0.0001), independent of the LVEF and QRS width. Of patients with a QRS width <120 ms (55%; n = 57), 56% presented with major intra-LV asynchrony and 12% with inter-V asynchrony. Intra-LV asynchrony was observed in 84% of left bundle branch block patients, but also in 83% of right bundle branch block patients (p = NS). There was a poor correlation between the QRS width and intra-LV or inter-V asynchrony (r = 0.36, p = NS and r = 0.43, p = 0.05, respectively). CONCLUSIONS: In HF patients without MI, patients with intra-LV asynchrony are those with a significantly higher risk of cardiac events, independent of the QRS width and LVEF. Accordingly, such patients should be more actively identified for early intensive treatment and survey. 相似文献
75.
76.
77.
Romuald Seizeur Elsa Magro Sylvain Prima Nicolas Wiest-Daesslé Camille Maumet Xavier Morandi 《Surgical and radiologic anatomy : SRA》2014,36(2):111-124
Purpose
Cerebral hemispheres represent both structural and functional asymmetry, which differs among right- and left-handers. The left hemisphere is specialised for language and task execution of the right hand in right-handers. We studied the corticospinal tract in right- and left-handers by diffusion tensor imaging and tractography. The present study aimed at revealing a morphological difference resulting from a region of interest (ROI) obtained by functional MRI (fMRI).Methods
Twenty-five healthy participants (right-handed: 15, left-handed: 10) were enrolled in our assessment of morphological, functional and diffusion tensor MRI. Assessment of brain fibre reconstruction (tractography) was done using a deterministic algorithm. Fractional anisotropy (FA) and mean diffusivity (MD) were studied on the tractography traces of the reference slices.Results
We observed a significant difference in number of leftward fibres based on laterality. The significant difference in regard to FA and MD was based on the slices obtained at different levels and the laterality index. We found left-hand asymmetry and right-hand asymmetry, respectively, for the MD and FA.Conclusions
Our study showed the presence of hemispheric asymmetry based on laterality index in right- and left-handers. These results are inconsistent with some studies and consistent with others. The reported difference in hemispheric asymmetry could be related to dexterity (manual skill). 相似文献78.
Complex karyotype in mantle cell lymphoma is a strong prognostic factor for the time to treatment and overall survival,independent of the MCL international prognostic index
下载免费PDF全文
![点击此处可从《Genes, chromosomes & cancer》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Fabrice Jardin Isabelle Radford Catherine Roche‐Lestienne Dominique Penther Christian Bastard Sophie Rigaudeau Sylvain Pilorge Franck Morschhauser Didier Bouscary Richard Delarue Hassan Farhat Philippe Rousselot Olivier Hermine Hervé Tilly Sylvie Chevret Sylvie Castaigne 《Genes, chromosomes & cancer》2014,53(1):106-116
Mantle cell lymphoma (MCL) is usually an aggressive disease. However, a few patients do have an “indolent” evolution (iMCL) defined by a long survival time without intensive therapy. Many studies highlight the prognostic role of additional genetic abnormalities, but these abnormalities are not routinely tested for and do not yet influence the treatment decision. We aimed to evaluate the prognostic impact of these additional abnormalities detected by conventional cytogenetic testing, as well as their relationships with the clinical characteristics and their value in identifying iMCL. All consecutive MCL cases diagnosed between 1995 and 2011 at four institutions were retrospectively selected on the basis of an informative karyotype with a t(11;14) translocation at the time of diagnosis. A total of 125 patients were included and followed for an actual median time of 35 months. The median overall survival (OS) and survival without treatment (TFS) were 73.7 and 1.3 months, respectively. In multivariable Cox models, a high mantle cell lymphoma international prognostic index score, a complex karyotype, and blastoid morphology were independently associated with a shortened OS. Spleen enlargement, nodal presentation, extra‐hematological involvement, and complex karyotypes were associated with shorter TFS. A score based on these factors allowed for the identification of “indolent” patients (median TFS 107 months) from other patients (median TFS: 1 month). In conclusion, in this multicentric cohort of MCL patients, a complex karyotype was associated with a shorter survival time and allowed for the identification of iMCL at the time of diagnosis. © 2013 Wiley Periodicals, Inc. 相似文献
79.
Genetic Variations and Diseases in UniProtKB/Swiss‐Prot: The Ins and Outs of Expert Manual Curation
下载免费PDF全文
![点击此处可从《Human mutation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Arnaud Gos Jerven Bolleman Sébastien Géhant Lionel Breuza Alan Bridge Sylvain Poux Nicole Redaschi Lydie Bougueleret Ioannis Xenarios the UniProt Consortium 《Human mutation》2014,35(8):927-935
During the last few years, next‐generation sequencing (NGS) technologies have accelerated the detection of genetic variants resulting in the rapid discovery of new disease‐associated genes. However, the wealth of variation data made available by NGS alone is not sufficient to understand the mechanisms underlying disease pathogenesis and manifestation. Multidisciplinary approaches combining sequence and clinical data with prior biological knowledge are needed to unravel the role of genetic variants in human health and disease. In this context, it is crucial that these data are linked, organized, and made readily available through reliable online resources. The Swiss‐Prot section of the Universal Protein Knowledgebase (UniProtKB/Swiss‐Prot) provides the scientific community with a collection of information on protein functions, interactions, biological pathways, as well as human genetic diseases and variants, all manually reviewed by experts. In this article, we present an overview of the information content of UniProtKB/Swiss‐Prot to show how this knowledgebase can support researchers in the elucidation of the mechanisms leading from a molecular defect to a disease phenotype. 相似文献
80.
Usefulness of 2‐[18F]‐fluoro‐2‐deoxy‐d‐glucose–Positron Emission Tomography/Computed Tomography for Staging and Evaluation of Treatment Response in IgG4‐Related Disease: A Retrospective Multicenter Study
下载免费PDF全文
![点击此处可从《Arthritis care & research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Mikael Ebbo Aurélie Grados Eric Guedj Delphine Gobert Cécile Colavolpe Mohamad Zaidan Agathe Masseau Fanny Bernard Jean‐Marie Berthelot Nathalie Morel François Lifermann Sylvain Palat Julien Haroche Xavier Mariette Bertrand Godeau Emmanuelle Bernit Nathalie Costedoat‐Chalumeau Thomas Papo Mohamed Hamidou Jean‐Robert Harlé Nicolas Schleinitz 《Arthritis care & research》2014,66(1):86-96