Intravenous infusion of propofol for induction and maintenance of anaesthesia during endoscopic carbon dioxide laser ENT procedures with high frequency jet ventilation

Fourteen patients of ASA grades 1 3 were anaesthetised with continuous infusions of propofol and alfentanil for endoscopic carbon dioxide laser ENT microsurgery. Their lungs were ventilated with an oxygen-air mixture using a high frequency jet ventilator. Propofol was given at an initial rate of 120 μg/kg/minute for 10 minutes after a bolus dose of 2.6 mg/kg, and then at 80 fig μg/kg/minute. Alfentanil was given at a rate of 0.5 μg/kg/minute. Arterial pressure decreased significantly after the bolus dose. It increased significantly for a few minutes after laryngoscopy and returned to baseline values during maintenance of anaesthesia. Heart rate increased significantly during induction and until laryngoscopy was performed but it decreased below its initial value after 5 minutes of maintenance. Platelet count and the degree of aggregation did not change during infusion of propofol.     

Autologous melanocyte–keratinocyte suspension in the treatment of vitiligo

Background In stable vitiligo, several techniques of autologous transplantation of melanocytes are used. Autologous melanocyte transplantation of non‐cultured melanocytes is one of those techniques with variable reported outcomes. Objective The objective of this study was to evaluate the response to autologous melanocyte–keratinocytes suspension transplantation in cases of stable vitiligo. Methods A total of 25 cases of vitiligo were treated by autologous melanocyte–keratinocytes suspension transplantation. After 6–17 months, patients’ response was evaluated according to the extent of pigmentation (excellent 90–100%, good 50–89%, fair 20–49% and poor response <20%). Results Of the 25 patients treated, 22 continued the follow‐up period. Five (23%) patients showed excellent response, 7 (32%) good, 6 (27%) fair and 4(18%) showed poor response. Conclusion Unlike transplantation of cultured melanocytes, which requires experience in culture technique, autologous melanocyte–keratinocytes suspension transplantation is an easy economic technique, which may be used in resistant areas of stable vitiligo.     

Broadening the spectrum of controls for skin biopsy in painful neuropathies

Epidermal nerve fiber density (ENFD) is useful in the evaluation of small‐fiber neuropathies (SFSNs). A recent evidence‐based review highlighted the need to broaden the spectrum of ENFD controls to include lower limb pain states other than polyneuropathy. We studied epidermal innervation in multiple sclerosis (MS) and distal lower limb burning pain (DLLBP). Distal‐leg ENFD/morphology in MS DLLBP patients did not differ significantly from that of healthy controls. This study extends the range of ENFD controls and further supports use of ENFD assessment in SFSN. Muscle Nerve, 2010     

腓骨肌萎缩症1A型基因重复的检测

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Male versus female lupus: a comparison of ethnicity, clinical features, serology and outcome over a 30 year period

Objective: To review the differences between male and female lupus patients with respect to clinical features, serology and outcome over a thirty year period. Material and methods: An observational study of all SLE patients seen at University College of London Hospital between 1976 and 2005 was performed. Demographic, clinical and serological data and outcome were retrospectively collected from hospital records or questionnaires and reviewed. Comparisons between continuous variables were made using the Kruskal-Wallis test and Student's t-test. Chi-square test or Fisher′s exact test were used for categorical variables when it was appropriate. Results: A total of 484 patients (439 females and 45 males) were identified between 1976 and 2005. Their mean age at diagnosis was 29.3 years (SD 12.6) with no significant differences between men and women. There were no significant differences between the number of men and women diagnosed over the different decades or in the mean age at diagnosis. Female gender was significantly associated with the presence of oral ulcers (29.2% vs. 13.3%, p?相似文献   

结核病小鼠病理学与TH1/TH2细胞动力学及iNOS表达关系的研究

目的 从分子病理学角度探讨结核病小鼠病理学与免疫学的相关性。方法 将BALB／c小鼠随机分为两组：结核病模型组（A）和正常对照组（N）。经尾静脉注射H37RV建立结核病小鼠模型。采用免疫组化染色和常规HE染色,探讨IFN－γ、IL－4、iNOS表达与肺脏组织损伤的类型和程度的关系。免疫组化结果采用图象分析计算阳性细胞面密度。结果 结核病小鼠病程中可观察到两个阶段：感染2周内为急性炎症明,以肺泡－毛细血管间质和血管周围炎性渗出为特征,可观察到明显的TH1细胞占优势,免疫组织化学染色结果显示,在炎性渗出区内IFN－γ阳性细胞较IL－4阳性细胞明显增多;感染第4周到实验结束为慢性进展期,以肺实质内广泛肺炎伴灶性干酷坏死为特征,免疫反应表现为THO平衡,在肺损伤组织中IFN－γ和IL－4阳性细胞基本相当。iNOS表达在急性期增多慢性期减少。结核病感染过程中TH1／TH2细胞动力学和iNOS3表达与组织损伤的类型和程度关系密切。