高山红景天多糖对小鼠抗柯萨奇B5病毒感染能力的研究

对感染柯萨奇Ｂ５病毒（ＣＶＢ）小鼠模型研究结果表明，高山红景天多糖对发病小鼠心肌功能改善和免疫功能提高均具有明显的促进作用。增强了小鼠抗ＣＶＢ５感染的能力，并对由ＣＢＶ感染性疾病也有一定的防治作用。     

体外反搏治疗失血性休克中一氧化氮合酶的变化

目的：探讨反搏治疗失血休克中一氧化氮合酶（ＮＯＳ）的变化。方法：复制狗的失血休克模型，用同位素方法测定反搏前后各组织的ＮＯＳ活性。结果：体外反搏后平均动脉压较反搏前明显上升（Ｐ＜００１）。脑ＮＯＳ测定值假手术对照组明显高于失血休克组Ｐ＜００１）及失血休克克反搏组（Ｐ＜００１），失血休克组则明显低于失血休克反搏组（Ｐ＜００１）；心肌ＮＯＳ活性假手术对照组与失血休克反搏组均明显高于失血休克组（Ｐ＜００５，Ｐ＜００１），但假手术对照组与失血休克反搏组之间无显著差异（Ｐ＞００５）；主动脉ＮＯＳ活性假手术对照组明显高于失血休克组（Ｐ＜００１）及失血休克反搏组（Ｐ＜００５），但失血休克组与失血休克反搏组间无明显差异（Ｐ＞００５）。结论：体外反搏可以增强小血管ＮＯＳ活性，ＮＯＳ活性的恢复则可能在反搏治疗中起重要作用     

Possible role of a proteinase in endosporulation of Coccidioides immitis.

We previously reported isolation of a serine proteinase from the soluble conidial wall fraction of Coccidioides immitis. The purified proteinase was identified as a polypeptide band of 36,000 Mr by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In this study, we raised monospecific antiserum in rabbits against the purified proteinase for use in immunoelectron microscopy. We showed that immunolabel was localized in the cell wall of both the saprobic and parasitic phases but was most concentrated in the wall of the segmentation apparatus of spherules just prior to endospore differentiation. The total wall fractions of the mycelial phase, as well as those of presegmented and endosporulating spherules, were isolated from in vitro grown cells and then treated with a proteinase inhibitor (phenylmethylsulfonyl fluoride [PMSF]) which irreversibly binds to the residual proteolytic enzyme in the wall isolates. Each fraction was dialyzed, lyophilized, and separately incubated with the active, purified 36,000-Mr proteinase. The reaction mixtures were examined spectrophotometrically (A280) for decomposition of the substrates. Only the PMSF-treated wall isolated from endosporulating spherules was significantly digested. Active, 36,000-Mr proteinase was isolated from intact and viable, endosporulating spherules by brief extraction of the cells with 1% octyl-beta-D-thioglucoside, a nonionic detergent. The serine proteinase may be partly responsible for autolysis of the segmentation apparatus of mature spherules, a morphogenetic process which is pivotal for release of endospores and subsequent proliferation of the pathogen.     

TIPE2 Ki67在子宫内膜癌组织中的表达及对预后的评估价值

目的 探讨肿瘤坏死因子诱导蛋白8样蛋白2(TIPE2)、细胞增殖核抗原Ki67在子宫内膜癌组织中的表达及对患者预后的评估价值。方法 选取2018年1月至2020年1月河北工程大学附属医院妇科收治的子宫内膜癌患者120例作为观察组,另选取同期收治的非子宫内膜癌患者130例作为对照组,所有入组患者均接受手术治疗。回顾性收集患者的临床及病理资料,比较两组患者子宫内膜组织中TIPE2、Ki67的表达水平。同时定期对观察组患者进行随访,分析患者2年生存情况,通过Cox比例风险回归模型分析子宫内膜癌患者预后的影响因素。结果 观察组、对照组子宫内膜组织中TIPE2阳性率分别为28.33%、71.54%。与对照组相比,观察组子宫内膜组织TIPE2阳性率降低（P<0.05）;观察组、对照组子宫内膜组织中Ki67阳性率分别为33.33%、5.38%。观察组子宫内膜组织Ki67阳性率高于对照组（P<0.05）。对120例子宫内膜癌患者随访2年,至随访结束,93例患者存活,生存率77.50%。Cox模型分析结果显示,TIPE2阴性（HR=3.736,95%CI=1.016～13.157）为子宫内膜...     

利用计算机对肺及纵膈肿瘤照射野的设计探讨

胸部肿瘤以往多采用二野前后对穿照射及前后野加水平野照射,使其脊髓及胸骨受量偏高,纵膈剂量低,高剂量区常位于靶区之外,剂量分布不合理。放射治疗时,要求减少脊髓,正常肺及胸骨的受量(后者尤其对青少年),提高靶区的致死量。作者利用Philips-oss治疗计划系统,选择肺癌及纵膈肿瘤各一例。根据胸部的解剖位置,采用三野成角加楔形板,经治疗计划系统内存参数处理后,绘制出剂量分布曲线,其优点是靶区内剂量分布均匀。在肺癌及纵膈肿瘤达到致死量的同时,脊髓,正常肺组织及胸骨受量减少或为零。     

Elimination of polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs) from human blood in the Yusho and Yu-Cheng rice oil poisonings

The pharmacokinetics of polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs) in humans was studied by monitoring the blood concentrations of individuals who ingested a contaminated rice oil in Japan (yusho) in 1968 and in Taiwan (yu-cheng) in 1979. Sixteen yusho patients were followed from 1982 to 1990 and three yu-cheng individuals from 1980 to 1989. From the three yucheng patients, blood lipid values for the two persistent toxic congeners, 2,3,4,7,8-pentachlorodibenzofuran (PnCDF) and 1,2,3,4,7,8-hexachlorodibenzofuran (HxCDF), varied from 50 g/kg at first sampling to about 1 g/kg at last sampling corresponding to half-lives for elimination (t_1/2) of 2-2_1/2 years. The blood lipid values for the same PCDF congeners in yusho patients varied from 5 g/kg down to 0.1 g/kg. The calculated t_1/2 were more variable with median values closer to 10 years. Planar PCBs #126 and #169 were present at lower concentrations than the PCDFs. For seven of the other PCB congeners, half-lives for elimination in the yu-cheng individuals varied from 1.2 up to 4.6 yr depending on the degree of chlorination. For the yusho patients, the elimination for the PCBs was longer. These results show that clearance of the toxic PCDFs and PCBs in humans is non-linear with faster elimination at higher exposure followed by slower decreases as background levels are approached. Such a clearance pattern can best be explained by a two compartment liver and fat pharmacokinetic model.     

Estimation of neutron dose equivalent at the mezzanine of the Advanced Light Source and the laboratory boundary using the ORNL program MORSE

To investigate the radiation effect of neutrons near the Advanced Light Source (ALS) at Lawrence Berkeley Laboratory (LBL) with respect to the neutron dose equivalents in nearby occupied areas and at the site boundary, the neutron transport code MORSE, from Oak Ridge National Laboratory (ORNL), was used. These dose equivalents result from both skyshine neutrons transported by air scattering and direct neutrons penetrating the shielding. The ALS neutron sources are a 50-MeV linear accelerator and its transfer line, a 1.5-GeV booster, a beam extraction line, and a 1.9-GeV storage ring. The most conservative total occupational-dose-equivalent rate in the center of the ALS mezzanine, 39 m from the ALS center, was found to be 1.14 X 10(-3) Sv y-1 per 2000-h "occupational" year, and the total environmental-dose-equivalent rate at the ALS boundary, 125 m from the ALS center, was found to be 3.02 X 10(-4) Sv y-1 per 8760-h calendar year. More realistic dose-equivalent rates, using the nominal (expected) storage-ring current, were calculated to be 1.0 X 10(-4) Sv y-1 and 2.65 X 10(-5) Sv y-1 occupational year and calendar year, respectively, which are much lower than the DOE reporting levels.     

Structures of four new isoprenylated xanthones, cudraxanthones h,i,j, and k1,2

Four new isoprenylated xanthones, cudraxanthone H,I,J, and K were isolated from the ethanol extract of the root bark of CUDRANIA TRICUSPIDATA (Carr.) Bur. (Moraceae), collected in China. The structures of cudraxanthones H,I,J, and K were shown to be 1- 4, respectively, on the basis of chemical and spectral evidence.     

Structures of three new isoprenylated xanthones, cudraxanthones e, f, and g 1,2

Three new isoprenylated xanthones, cudraxanthones E, F, and G along with a known xanthone, 6-deoxyisojacareubin, were isolated from the ethanol extract of the root bark of CUDRANIA TRICUSPIDATA (Moraceae), collected in China. The structures of cudraxanthones E, F, and G were shown to be 1-3, respectively, on the basis of spectral evidence.