分光光度法测定金蝉花中多糖的含量

目的建立金蝉花药材中多糖的含量测定方法.方法采用分光光度法测定多糖含量.结果金蝉花中多糖含量为24.16 mgg-1,平均回收率为97.37%,RSD＝2.34%,n＝5.结论本法简便、准确、灵敏、重现性好,为金蝉花药材的质量控制提供了依据.     

Genetic control of the circulating concentration of transforming growth factor type beta1

The concentration of transforming growth factor beta (TGF-beta) in plasma has been correlated with the development of several diseases, including atherosclerosis and certain forms of cancer. However, the mechanisms that control the concentration of TGF-beta in plasma are poorly understood. In a study of 170 pairs of female twins (average age 57.7 years) we show that the concentration of active plus acid- activatable latent TGF-beta1 [(a+l) TGF-beta therefore is predominantly under genetic control (heritability estimate 0.54). Single strand conformation polymorphism (SSCP) mapping of the TGF-beta1 gene promoter has identified two single base substitution polymorphisms. The two polymorphisms (G-->A at position -800 bp and C-->T at position -509 bp) are in linkage disequilibrium (correlation coefficient Delta = 0.215, P < 0.01). The C-509T polymorphism is significantly associated with the plasma concentration of (a+l) TGF-beta1, explaining 8.2% of the additive genetic variance of (a+l) TGF-beta1 concentration. It is therefore possible that predisposition to atherosclerosis, bone diseases or various forms of cancer may be correlated with the presence of particular alleles at the TGFB1 locus.      

Electrical impedance tomography compared to positron emission tomography for the measurement of regional lung ventilation: an experimental study

Introduction  

Electrical impedance tomography (EIT), which can assess regional lung ventilation at the bedside, has never been compared with positron-emission tomography (PET), a gold-standard to quantify regional ventilation. This experiment systematically compared both techniques in injured and non-injured lungs.     

人精子膜甘露糖结合蛋白的研究--蛋白的定位

作者运用生物素标记的山羊抗人巨噬细胞甘露糖受体（ＧｏａｔＡｎｔｉ－ｈｕｍａｎＭａｃｒｏｐｈａｇｅＭａｎｎｏｓｅＲｅｃｅｐｔｏｒ,ＡＭＭＲ）ＩｇＧ对人获得能精子膜上的甘露糖结合蛋白进行定位,经激光共聚焦显微镜分析结果显示：ＡＭＭＲＩｇＧ组阳性率显著高于运用正常山羊（ＮｏｒｍａｌＧｏａｔ,ＮＧ）ＩｇＧ的对照组：ＡＭＭＲＩｇＧ组的阳性率大约在７０％（６９．４１％）左右,而ＮＧ ＩｇＧ对照组中除少量精子显     

Effect of RANTES on the infection of monocyte-derived primary macrophages by human immunodeficiency virus type 1 and type 2

The effect of β chemokines on human immunodeficiency virus type I (HIV-1) infection of primary macrophages is controversial, and their effect on HIV-2 infection of these cells has not yet been documented. We examined the effect of synthetic and recombinant regulated-on-activation, normal T cell-expressed and -secreted (RANTES) on HIV-1 and HIV-2 infection of primary monocyte-derived-macrophages (MDM) that were obtained as the adherent cells of 5-day cultures of blood mononuclear cells (PBMC), followed by 2-day culture without peripheral blood mononuclear cells (PBMCs) nor added cytokines. These MDM expressed CD4, CCR5 and CXCR4, the major coreceptors for HIV macrophage- and T cell-tropic isolates, respectively. Infection of MDM from different donors with HIV-1 or HIV-2 macrophagetropic strains was reproducibly inhibited by RANTES. This inhibition depended on RANTES continuous presence in culture during and after infection. Treatment of MDM with RANTES just before or during, but not after, exposure to virus did not protect MDM from infection. When RANTES was added after MDM had been infected, and was continuously maintained in culture thereafter, no inhibition occurred and limited enhancement of infection could be observed. These data indicate that RANTES inhibits HIV-1 as well as HIV-2 infection of MDM, likely at a post-binding step, and support the role of CCR5 as the major coreceptor for HIV-1 and HIV-2 entry into primary macrophages.     

LEE阴性STEC分离株IHA基因的筛选、克隆及序列分析

尽管类志贺毒素对于STEC感染的临床及病理表现的产生非常重要,但细菌的一些毒性因子,特别是与肠道定居和粘附相关的因子也是病变产生的关键性决定因子。我们通过Southern杂交、克隆、打点杂交和序列测定等方法,获得了LEE阴性STEC O113:H7分离株98NK2染色体上大小为5.2kb的Hind III酶切片段的完全DNA序列,其中包含的一个大小为2088bp的开放阅读框架IhaO113(IrgA homologue adhesin)与LEE阳性STEC O157:H21分离株染色体上的一个假定粘附素IhaO157的核酸序列具有93%的一致性,两者的推导氨基酸序列具有91%的一致性。本研究首次报道了LEE阴性STEC O113:H7分离株染色体上预期与细菌粘附相关的编码基因Iha的完全DNA序列,为进一步研究其致病的分子机制提供了重要的理论依据。     

HIV-1-infection in a man who has sex with men despite self-reported excellent adherence to pre-exposure prophylaxis,the Netherlands,August 2021: be alert to emtricitabine/tenofovir-resistant strain transmission

In August 2021, a man who has sex with men was diagnosed with HIV-1 infection despite using event-driven pre-exposure prophylaxis for over 2 years with self-reported excellent adherence. Sequencing identified resistance-associated mutations (RAM) M184V and K65R, conferring resistance to emtricitabine and tenofovir, and RAM V108I and E138A. Background RAM prevalence was two of 164 (1.2%) new HIV diagnoses in Amsterdam (2017–19). We reiterate the need for frequent HIV testing among PrEP users and additional testing in case of symptoms.     

消化道肿瘤肝转移的介入治疗

介入治疗方法是一种微创手术,具有损伤小、近期疗效显著、并发症少、易于重复等特点,愈来愈得到国内外学者的重视.目前,肝动脉栓塞化疗、瘤内无水酒精注射等介入治疗已经得到了广泛应用,各种新技术不断应用于转移性肝癌介入治疗,并显示了较好的治疗效果.转移性肝癌的介入治疗方法多种,主要包括经皮的局部消蚀法和经血管的介入方法两大类.     

DOUBLE-BLIND STUDY COMPARING THE LONG-TERM EFFICACY OF THE COX-2 INHIBITOR NIMESULIDE AND NAPROXEN IN PATIENTS WITH OSTEOARTHRITIS

This double-blind one-year study compares the long-term efficacy and safety of nimesulide with naproxen in patients with osteoarthritis (OA) of the knee or hip. Patients were randomised to nimesulide 100 mg twice daily (n=183) or naproxen 250 mg morning, 500 mg evening (n=187). The primary efficacy variable was change in pain intensity (WOMAC A scale) at 6 months. Nimesulide tablets showed at least equivalent efficacy to naproxen tablets in reducing pain intensity at 6 and 12 months (nimesulide -22.5% at 6 and 12 months; naproxen -22.4% at 6 months, -19.9% at 12 months; non-inferiority proven). At 6 months the investigator assessed efficacy as ‘good’ or ‘excellent’ in 59.3% of nimesulide and 56.4% of naproxen-treated patients, with corresponding values for patient assessment of 57% and 52.7%. Both treatments were well tolerated, with fewer related gastrointestinal adverse events reported with nimesulide (77 cases, 47.5%) than with naproxen (96 cases, 54.5%). This study shows nimesulide to be as effective as naproxen in the long-term treatment of OA and to be associated with fewer gastrointestinal side-effects.