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991.
Restaíno CG Chaparro A Valenzuela MA Kettlun AM Vernal R Silva A Puente J Jaque MP León R Gamonal J 《Oral diseases》2007,13(5):474-481
OBJECTIVE: Neutrophils play a crucial role in the defense of invading bacteria by releasing biologically active molecules. The response of peripheral blood neutrophils was studied in periodontitis-affected patients and in healthy controls towards stimulation to Porphyromonas gingivalis (Pg) and Actinobacillus actinomycetemcomitans (Aa) extracts. MATERIALS AND METHODS: Peripheral venous blood was drawn from 23 adult patients with moderate to advanced chronic periodontitis (probing depth >or=5 mm, attachment loss >or=3 mm), and 30 healthy volunteers. Neutrophil response followed by metalloproteinase-9 (MMP-9) and interleukin-8 (IL-8) secretion was assayed by zymography and enzyme-linked immunosorbent assay, respectively, on both whole blood and purified neutrophils. In addition to periodontal pathogen extracts, known stimulating agents were tested, such as Escherichia coli-lipopolysaccharide (LPS), phytohemagglutinin, and zymosan A. RESULTS: Neutrophil response, expressed as a secretion ratio under stimulated and non-stimulated conditions, measured in whole blood, showed no differences between periodontitis and healthy controls. Instead, in purified neutrophils from patients, MMP-9 exhibited a significantly higher secretion ratio with LPS and Pg (1.5- to 2-fold), whereas IL-8 showed a larger increase in secretion ratio (3- to 7-fold) in the presence of Pg, Aa, LPS, and zymosan A. CONCLUSION: Peripheral neutrophils of periodontitis-affected patients are more reactive as suggested by their significantly higher response toward periodontal pathogen extracts and other stimulating agents. 相似文献
992.
993.
Identification of the Molecular Determinants of the Antibacterial Activity of LmutTX,a Lys49 Phospholipase A2 Homologue Isolated from Lachesis muta muta Snake Venom (Linnaeus, 1766)
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Rafaela Diniz‐Sousa Cleópatra A. S. Caldeira Anderson M. Kayano Mauro V. Paloschi Daniel. C. Pimenta Rodrigo Simões‐Silva Amália S. Ferreira Fernando B. Zanchi Najla B. Matos Fernando P. Grabner Leonardo A. Calderon Juliana P. Zuliani Andreimar M. Soares 《Basic & clinical pharmacology & toxicology》2018,122(4):413-423
Snake venom phospholipases A2 (PLA2s) are responsible for numerous pathophysiological effects in snakebites; however, their biochemical properties favour antimicrobial actions against different pathogens, thus constituting a true source of potential microbicidal agents. This study describes the isolation of a Lys49 PLA2 homologue from Lachesis muta muta venom using two chromatographic steps: size exclusion and reverse phase. The protein showed a molecular mass of 13,889 Da and was devoid of phospholipase activity on an artificial substrate. The primary structure made it possible to identify an unpublished protein from L. m. muta venom, named LmutTX, that presented high identity with other Lys49 PLA2s from bothropic venoms. Synthetic peptides designed from LmutTX were evaluated for their cytotoxic and antimicrobial activities. LmutTX was cytotoxic against C2C12 myotubes at concentrations of at least 200 μg/mL, whereas the peptides showed a low cytolytic effect. LmutTX showed antibacterial activity against Gram‐positive and Gram‐negative bacteria; however, S. aureusATCC 29213 and MRSA strains were more sensitive to the toxin's action. Synthetic peptides were tested on S. aureus, MRSA and P. aeruginosaATCC 27853 strains, showing promising results. This study describes for the first time the isolation of a Lys49 PLA2 from Lachesis snake venom and shows that peptides from specific regions of the sequence may constitute new sources of molecules with biotechnological potential. 相似文献
994.
995.
de Oliveira Assunção Flávia Fernanda Nascimento Érika Chaves Lucas da Silva Alessandro Márcio Hakme Martinez Roberto de Jesus Guirro Rinaldo Roberto 《Lasers in medical science》2022,37(5):2439-2447
Lasers in Medical Science - Photobiomodulation has been used to inactivate bacterial growth, in different laser or LED protocols. Thus, the aim of this study was to verify the inhibition of... 相似文献
996.
Carneiro Alexandra Melo Pingarilho Barros Antonia Patrícia Oliveira de Oliveira Roberta Pimentel de Paula Brennda Lucy Freitas Silva Aryvelto Miranda de Melo Alencar Cristiane Silva Cecy Martins 《Lasers in medical science》2022,37(7):2791-2804
Lasers in Medical Science - The authors aimed to conduct a systematic review to assess data from the current literature on the effectiveness of low-level laser therapy (LLLT) in preventing tooth... 相似文献
997.
Carboni Rosadélia Malheiros Gonçalves Marcela Leticia Leal Tacla Elaine Marlene Silva Daniela Fátima Teixeira Bussadori Sandra Kalil Fernandes Kristianne Porta Santos Horliana Anna Carolina Ratto Tempestini Mesquita-Ferrari Raquel Agnelli 《Lasers in medical science》2022,37(3):1881-1890
Lasers in Medical Science - The benefits of photobiomodulation (PBM) applied to wounds are well-described in the literature; however, its effects in skin graft donor sites have been poorly studied.... 相似文献
998.
Ibarra Ana Melissa Ccopa Cecatto Rebeca Boltes Motta Lara Jansiski dos Santos Franco Adriana Lino de Fátima Teixeira da Silva Daniela Nunes Fabio Daumas Hamblin Michael R Rodrigues Maria Fernanda Setúbal Destro 《Lasers in medical science》2022,37(3):1441-1470
Lasers in Medical Science - This narrative review aimed to evaluate the effectiveness of PDT in early or advanced squamous cell carcinoma of the head and neck (SCCHN). Scopus, MEDLINE/PubMed, and... 相似文献
999.
Lasers in Medical Science - Stress urinary incontinence (SUI) is a common health problem that affects roughly 35% of women in the reproductive period. A prospective uncontrolled study was conducted... 相似文献
1000.
Marzano Lucas Alexandre Santos Batista Joao Pedro Thimotheo de Abreu Arruda Marina de Freitas Cardoso Mara Glria de Barros Joo Lus Vieira Monteiro Moreira Janana Matos Liu Priscila Menezes Ferri Teixeira Antnio Lcio Simes e Silva Ana Cristina de Miranda Aline Silva 《Neurosurgical review》2022,45(1):167-197
Neurosurgical Review - Traumatic brain injury (TBI) is the main cause of pediatric trauma death and disability worldwide. Recent studies have sought to identify biomarkers of TBI for the purpose of... 相似文献