雷帕霉素联合顺铂对CCL19调节头颈鳞状细胞癌细胞活性的影响

目的 探讨雷帕霉素(rapamycin)与顺铂(cisplatin)联合使用对趋化因子CCL19调节头颈鳞状细胞癌细胞活性的影响.方法 应用甲基噻唑基四唑(MTT)法及流式细胞仪检测顺铂及雷帕霉素对CCL19诱导后人头颈鳞状细胞癌淋巴结转移细胞系(PCI-4B、PCI-37B)生长抑制作用、细胞凋亡作用和细胞周期变化.使用Calcusyn软件计算两种药物(浓度比为80:1)联合使用时的剂量效应参数及联合指数(combination index,CI),使用SPSS 11.0软件包进行统计分析.结果 顺铂及雷帕霉素可以分别提高CCL19作用后细胞的生长抑制率(CCL19+顺铂作用后两细胞系的生长抑制率为21.22%±2.68%和22.76%±2.79%,CCL19+雷帕霉素作用后为19.46%±2.54%和20.6%±4.38%)、细胞G1期比例(CCL19+顺铂作用后两细胞系的G1期比例为72.14%±2.66%和76.39%±1.45%,CCL19+雷帕霉素作用后为75.26%±5.92%和74.00%±1.51%)和凋亡率(CCL19+顺铂作用后两细胞系的凋亡率9.59%±0.86%和7.55%±0.66%,CCL19+雷帕霉素作用后为8.21±1.80%和6.26±0.78%).两种药物浓度比为80:1联合使用时,PCI-4B细胞在半数抑制量(inhibitory concentration 50%,IC50)以下,CI＜1,IC75以上CI＞1;PCI-37B则在IC75以下CI＜1,IC90以上CI＞1.结论 顺铂及雷帕霉素均有抑制CCL19对人头颈鳞状细胞癌淋巴结转移细胞的活性调节作用,并且二者联合使用存在协同作用.

Abstract：

Objective To investigate the synergistic effects of rapamycin and cisplatin on head and neck squamous cancer cells regulated by chemokine( C-C motif) ligand 19 (CCL19). Methods The role of rapamycin and cisplatin was detected on cell-cycle and apoptosis in CCL19 induced PCI-4B and PCI-37B cells by methyl thiazolyl tetrazolium (MTT) and flow cytometry(FCM). Dose-effect relationship parameters and combination index(CI) were calculated on the median-effect equation and multiple drug effect equation using computer software CalcuSyn. Statistical analysis was performed by the unpaired student's t-test.Results Rapamycin and cisplatin could respectively increase the growth arrest, the proportion of G1 phase and apoptosis of CCL19 induced cancer cells (P ＜0. 05 ). Under inhibitory concentration 50% (IC50), CI was less than 1, and in IC75, it was more than 1 in PCI-4B cells. In PCI-37B cells, under IC75, CI was less than 1, and in IC90, it was more than 1. Conclusions Rapamycin and cisplatin can inhibit CCL19-regulated PCI-4B and PCI-37B cells' survival. The two drugs have synergistic effects when used in combination.     

Relationship between vitamin D receptor gene polymorphism and periodontitis

Recent studies have shown that vitamin D receptor (VDR) gene polymorphism had regulatory effects on bone mineral density (BMD) and bone turnover. The VDR gene has also been indicated as a candidate gene for the susceptibility of osteoporosis. However, it is unclear whether VDR genotypes could be associated with alveolar bone loss of patients with periodontitis, or whether vitamin D receptor gene could be a candidate gene for susceptibility to periodontitis. The purpose of this study was to answer these two questions. METHODS: Twenty-four cases of adult periodontitis (AP), 37 cases of early onset periodontitis (EOP) and 39 healthy controls were recruited for the study. Individual samples of venous blood and DNA were obtained from each subject. Genotypes of the TaqI VDR gene were determined by PCR and TaqI restriction endonuclease digestion. RESULTS: One out of 24 AP patients, nine out of 37 EOP patients and two out of 39 healthy controls were detected with Tt genotype, while the rest had the TT genotype. The detected frequency of Tt genotype was significantly higher in EOP patients (24.3%) than in AP patients (4.2%) and healthy controls (5.1%). The frequency of t allele was also significantly higher in EOP patients. There was no statistical difference in the distribution of TaqI VDR genotypes between AP patients and healthy controls. The study suggests that Tt genotype might be a risk indicator for the susceptibility to EOP. Carriage of the allele (t) of the TaqI VDR gene may increase the risk of developing EOP.     

A comparative morphologic study of Carabelli cusp between Chinese and Japanese students.]

The study of teeth,dentition and occlusion is helpful to guide oral medical clinic and the study of anthropology.Our department of Osaka Dental University and had many items of research work about teeth,dentition and occlusion between Chinese and Japanese students.This article reports the study of Carabelli cusp.The results revealed:(1)the rate of Carabelli cusp is higher in Japanese than in Chinese and is higher in male than in female.(2)the rate of Carabelli cusp of the first maxillary molar of both side is very high.(3)The rate of Carabelli cusp is related to the height and the type of cusp numbers.These all illustrated the occurence of Carabeiil cusp were influenced by race, individual and the development of teeth.     

固齿健周煎剂对老年小鼠的抗氧化作用

目的探讨中药固齿健周煎剂对老年小鼠的抗氧化作用。方法实验组为固齿健周煎剂灌胃的老年小鼠，对照组为灌胃等量自来水的青、老年小鼠。检测过氧化脂质、超氧化物歧化酶及过氧化氢酶。结果实验组与青、老年小鼠对照组比较差异均有显著性。结论固齿健周煎剂具有抗脂质过氧化的作用，可能对调节机体代谢功能、抗自由基损伤及恢复牙周组织健康有益     

Clinical application of angiography of oral and maxillofacial hemangiomas. Clinical analysis of seventy cases

Angiographic findings in seventy cases of maxillofacial hemangioma are reported. The tumors have been divided into four types: (1) plexiform hemangioma, (2) cavernous hemangioma with venous drainage, (3) cavernous hemangioma without venous drainage, and (4) the so-called "diffuse type" of cavernous hemangioma. The angiographic features of the four types are described, and clinical application is evaluated. The angiograms demonstrate the location, extent, blood supply, and drainage of the tumors and show whether they communicate intracranially, thus providing valuable criteria for planning and determining the mode of treatment.     

口腔颌面部肿瘤化疗方案药物敏感性试验

尊文以人舌鳞癌细胞系Ｔｃａ８１１３为对象，利用ＭＴＴ法对口腔颌面部肿瘤化疗常用的八种药物和七种方案进行了药敏试验，ＡＤＭ、５－Ｆｕ、ＣＤＤＰ等抗肿瘤作用较强；而ＰＹＭ、ＭＴＸ、ＶＣＲ单用对Ｔｃａ８１１３细胞无明显作用。化疗方案中以ＭＭＢＤ、ＰＦ、ＰＶＰ方案为佳，卡铂与顺铂在此疗方案中的作用无明显差异。实验结果提示：肿瘤化疗不能只凭经验用药，应注意化疗的耐药性问题。     

Unilateral coronoid process atrophy associated with fatty degeneration of the masticatory muscles

Unilateral coronoid process atrophy associated with fatty degeneration of the masticatory muscles in a 76-year-old woman is reported. CT and plain radiogram findings are presented. The patient had no evidence of collagen disease which often causes osteolysis of the coronoid, process and condyle. Although the true cause of this deformity was unclear, a hypothesis of ischemic changes in the nutrient vessels was suggested as an etiologic factor.     

Levels of soluble cytokine factors in temporomandibular joint effusions seen on magnetic resonance images

OBJECTIVE: To elucidate the correlations between joint effusion (JE) on T2-weighted magnetic resonance images (MRI) of the temporomandibular joint (TMJ) and the levels of various cytokine receptors, cytokine antagonists, and protein in the synovial fluid of patients with temporomandibular joint disorders (TMD). STUDY DESIGN: Fifty-five TMJs of 55 patients with TMD were scanned by MRI, and synovial fluid samples were obtained on the same day. The grade of JE was evaluated on a scale of 0 to 3: Grades 0 and 1 indicated absence, and grades 2 and 3 indicated the presence of JE. Correlations were evaluated between JE and the concentrations of soluble tumor necrosis factor receptors I and II (sTNFR-I and sTNFR-II, respectively), IL-6 soluble receptor (IL-6sR), IL-1 soluble receptor type II, and IL-1 receptor antagonist and protein in the synovial fluid of patients with TMD. RESULTS: The concentrations of sTNFR-I and protein in the group with JE (18 joints) were significantly higher than in the group without JE (37 joints). In addition, there were significant positive correlations between the grade of JE and the levels of sTNFR-I, sTNFR-II, and protein. CONCLUSIONS: sTNFRs and protein may play important roles in the pathogenesis of TMD. These mediators seem to influence the expression of JE, which may reflect synovial inflammation of the TMJ.     

The effect of simvastatin on remodelling of the alveolar bone following tooth extraction

Suppression of residual ridge resorption after tooth extraction is a hot spot in dental research. Recently, simvastatin was reported to influence bone turnover by stimulating bone formation. In this study, the effect of simvastatin application on residual ridge resorption following tooth extraction was investigated. Sixty male Wistar rats were randomly divided into experimental and control groups (n=30). Polylactic acid/polyglycolic acid copolymer carriers, with or without simvastatin, were implanted into extraction sockets of right mandibular incisors. The rats were killed at 1, 2, 4, 8 or 12 weeks after implantation. The relative height of the residual alveolar ridge was significantly greater in the experimental compared to the control group at 2, 4, 8 and 12 weeks. The bone mineral density in the experimental group was significantly higher than that in the control group at 4, 8 and 12 weeks. A larger newly formed bone island was observed in the experimental group at 4 weeks, and higher bone formation rate and quality were found than in the control group at different time points except 1 week. The findings indicate that local application of simvastatin would effectively preserve the residual alveolar bone by promoting bone formation in the extraction socket.     

翼外肌上头与颞下颌关节盘关系的研究

目的探讨翼外肌上头与关节盘的关系。方法对４７侧老年尸体颞下颌关节标本进行了斜矢状面上的解剖学和组织学研究。结果①显微镜下观察翼外肌上头纤维可附着于关节盘、关节囊、关节盘前附着及髁突；②翼外肌上头附着于髁突的纤维平均为５２％，附着于关节盘的纤维为１０％，附着于关节囊的纤维为２４％，附着于下颌前附着的纤维量为４％；③翼外肌上头附着于关节盘的比例与关节盘前移无确切相关性；④翼外肌上头纤维与关节盘纤维的连接方式包括结点连接、肌腱连接和端端连接。结论翼外肌上头主要附着于髁突，附着于关节盘的少量纤维不足以独立地使关节盘发生前移位。