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211.
Kozako T Akimoto M Toji S White Y Suzuki S Arima T Suruga Y Matsushita K Shimeno H Soeda S Kubota R Izumo S Uozumi K Arima N 《Journal of medical virology》2011,83(3):501-509
Human T-cell lymphotropic virus type I (HTLV-1) causes adult T-cell leukemia/lymphoma and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The different patterns of clinical diseases are thought to be linked to immunogenetic host factors. A variety of autoimmune diseases, such as Sj?gren's syndrome, have been reported in persons infected with HTLV-1, although the precise relationship between these disorders and HTLV-1 infection remains unknown. There is no report on the repertoire of HTLV-1-specific CD8+ T-cells in HAM/TSP patients or carriers with autoimmune diseases, both characterized by an abnormal immune state. In this study, to characterize HTLV-1-specific CD8+ T-cells in asymptomatic HTLV-1 carriers, HAM/TSP patients and carriers with autoimmune diseases, we examined the frequency and diversity of HTLV-1-specific CD8+ T-cells using HTLV-1 tetramers. HTLV-1 Env-specific CD8+ T-cells were significantly more frequent in HAM/TSP and carriers with autoimmune diseases compared with asymptomatic HTLV-1 carriers, while the frequency of HTLV-1 Tax-specific CD8+ T-cells was not significantly different among them. CD8+ cells binding to HTLV-1 Tax tetramers in carriers with autoimmune diseases were significantly reduced compared with HAM/TSP patients. This study demonstrates the importance of CD8+ T-cells recognizing HTLV-1 Env-tetramers in HAM/TSP patients and carriers with autoimmune diseases, thereby suggesting that the diversity, frequency and repertoire of HTLV-1 Env-specific CD8+ T-cell clones may be related to the hyperimmune response in HAM/TSP and carriers with autoimmune diseases, although different immunological mechanisms may mediate the hyperimmunity in these conditions. 相似文献
212.
Shuji Yamamoto Hiroshi Nakase Minoru Matsuura Yusuke Honzawa Satohiro Masuda Ken‐ichi Inui Tsutomu Chiba 《Journal of gastroenterology and hepatology》2010,25(5):886-891
Background and Aim: Little is known about the efficacy and safety of infliximab for ulcerative colitis refractory to tacrolimus. The aim of this study was to evaluate the efficacy and safety of infliximab in the induction of remission in ulcerative colitis patients with persistent symptoms despite tacrolimus therapy. Methods: We report a retrospective, observational, single‐center case series of 12 consecutively enrolled patients with ulcerative colitis refractory to tacrolimus that received infliximab therapy for the induction of remission. Eight patients received a single infusion of infliximab, and four received two or more infusions. Median follow‐up duration was 16.0 months (range, 1.6–41.4 months). The clinical response was evaluated based on a modified Truelove‐Witts severity index. Results: Six patients (50.0%) achieved clinical remission within 30 days. Overall cumulative colectomy‐free survival was estimated to be 58.3% at 41.4 months. Adverse events included an elevation of liver enzymes (1/12; 8.3%) and a mild infusion reaction (1/12; 8.3%). No mortality occurred. Conclusions: Infliximab can induce remission in patients with ulcerative colitis who do not tolerate or respond to tacrolimus therapy. 相似文献
213.
Atsuko Niwa Masahiro Nishibori Shinichi Hamasaki Takuro Kobori Keyue Liu Hidenori Wake Shuji Mori Tadashi Yoshino Hideo Takahashi 《Brain structure & function》2016,221(3):1653-1666
In the adult hypothalamus and ependymal lining of the third ventricle, tanycytes function as multipotential progenitor cells that enable continuous neurogenesis, suggesting that tanycytes may be able to mediate the restoration of homeostatic function after stroke. Voluntary wheel running has been shown to alter neurochemistry and neuronal function and to increase neurogenesis in rodents. In the present study, we found that voluntary exercise improved the survival rate and energy balance of stroke-prone spontaneously hypertensive rats (SHRSP/Kpo). We also investigated the effect of exercise on the proliferation and differentiation of hypothalamic cells using immunoreactivity for tanycytes and neural markers. The proliferation of elongated cells, which may be the tanycytes, was enhanced in exercising SHRSP compared to sedentary rats before and after stroke. In addition, the proliferation of cells was correlated with the induction of fibroblast growth factor-2 in the subependymal cells of the third ventricle and in the cerebrospinal fluid. Some of the newborn cells of exercising SHRSP showed differentiation into mature neurons after stroke. Our results suggest that voluntary exercise correlates with hypothalamic neurogenesis, leading to recovery of homeostatic functions in the adult brain after stroke. 相似文献
214.
Changes in the number of activated sweat glands (ASGs) and sweat output per gland (SGO) with increased exercise intensity during sustained static exercise were investigated. Fourteen male subjects performed 20, 35, and 50% maximal voluntary contraction (MVC) for 60 s with the right hand (exercised arm) at an ambient temperature of 35 degrees C and 50% relative humidity. Although sublingual, local skin, and mean skin temperatures remained essentially constant throughout the exercise at each intensity, the sweating rate (SR) of nonglabrous skin on the nonexercised left forearm increased significantly with a rise in exercise intensity (p<0.05). Changes in the number of ASGs with rising exercise intensity paralleled changes in the SR, but the SGO did not change markedly with altered exercise intensity. These results suggest that in mildly heated humans, at less than 50% MVC, the increase in the SR from nonglabrous skin with rising exercise intensity during sustained static exercise is dependent on changes in the number of ASGs and not on SGO. 相似文献
215.
Hiroshi Inui Shuji Taketomi Ryota Yamagami Kohei Kawaguchi Keiu Nakazato Sakae Tanaka 《The Knee》2018,25(6):1247-1253
Background
Acquisition of appropriate anteroposterior (AP) stability depends on the prosthetic design and intraoperative soft tissue handling. A bi-cruciate stabilized (BCS) total knee arthroplasty (TKA) has a two cam-post mechanism, which substitutes for the anterior cruciate ligament and posterior cruciate ligament (PCL). Therefore, appropriate AP stability is expected. Because the PCL is sacrificed during BCS TKA, medial stability and lateral stability are thought to be important factors to determine AP stability. However, no previous study has reported AP stability after BCS TKA and the relationship between AP and medial–lateral stability.Methods
AP stability was measured using a navigation system intraoperatively and the KT 2000 device postoperatively. Intraoperative joint laxity of the medial and lateral compartments was evaluated separately using a compartment-specific ligament tensioner. The relationship between AP stability and medial–lateral laxity was assessed.Results
Intraoperative AP translation at 30° and 90° knee flexion angles was 7.7?±?3.1?mm and 5.9?±?2.0?mm, respectively. Postoperative AP translation at 30° was 5.9?±?1.7?mm. AP translation correlated positively with medial joint laxity at 30° (R?=?0.29) and 90° (R?=?0.40). The intraoperative and postoperative AP translations at 30° flexion had a positive relationship (R?=?0.61).Conclusion
AP stability of the BCS TKA had a positive relationship with intraoperative medial stability. Therefore, surgical soft tissue handling focusing on medial stability is also appropriate for AP stability of BCS TKA. Additionally, intraoperative AP translation turned out to be a predictive indicator for postoperative knee AP stability at 30° flexion. 相似文献216.
Shuji Shimizu Dai Une Toru Kawada Yohsuke Hayama Atsunori Kamiya Toshiaki Shishido Masaru Sugimachi 《The journal of physiological sciences : JPS》2018,68(2):103-111
The recent development of computer technology has made it possible to simulate the hemodynamics of congenital heart diseases on a desktop computer. However, multi-scale modeling of the cardiovascular system based on computed tomographic and magnetic resonance images still requires long simulation times. The lumped parameter model is potentially beneficial for real-time bedside simulation of congenital heart diseases. In this review, we introduce the basics of the lumped parameter model (time-varying elastance chamber model combined with modified Windkessel vasculature model) and illustrate its usage in hemodynamic simulation of congenital heart diseases using examples such as hypoplastic left heart syndrome and Fontan circulation. We also discuss the advantages of the lumped parameter model and the problems for clinical use. 相似文献
217.
Isao Takebe Etsuko Sawabe Kiyofumi Ohkusu Naoko Tojo Shuji Tohda 《Journal of clinical microbiology》2014,52(6):2251-2253
We report a case of catheter-related bloodstream infection by Tsukamurella inchonensis, identified using 16S rRNA gene sequencing, in a patient with myelofibrosis who underwent a bone marrow transplant. Tsukamurella species infections are rare. To our knowledge, this is the first case of T. inchonensis bloodstream infection in an immunocompromised patient. 相似文献
218.
Yoshinari Tanaka Hiroshi Mima Yasukazu Yonetani Yoshiki Shiozaki Norimasa Nakamura Shuji Horibe 《The Knee》2009,16(2):130-135
Although many surgical modalities for spontaneous osteonecrosis of the knee (SONK) of the medial femoral condyle have been reported, few reports have described these treatment options from the etiological point of view. Recently, osteochondral autografting has gained popularity for use in small cartilage injuries. The aims of this study were to characterize the SONK lesion histopathologically and to report on preliminary clinical results of autogenous osteochondral grafting for SONK.Six patients with SONK of the medial femoral condyle underwent osteochondral autografting. Average age was 54.2 years (range, 50–57 years). Using Koshino's classification, three patients' lesions were classified as stage III and three as stage IV. Classical histological investigation of the lesions was performed in all cases. All the patients achieved favorable pain relief after osteochondral autografts. The mean duration of follow-up was 27.7 months (range, 23–45 months). An increase in the average Lysholm score was found, ranging from 54.7 preoperatively to 92.3 postoperatively.Histological investigation of the lesions revealed articular bone plate fracture with enchondral ossification, reactive cartilage tissue formation, and proliferation of fibrous tissue. An area of osteonecrosis was observed in detached or fragmented osteochondral lesions.Osteochondral autografting was performed on six patients for the SONK and the short-term clinical results were favorable. Histological results give support to subchondral fracture as the etiological mechanism underlying SONK. 相似文献
219.
Shuji Mizumoto Andreas R. Janecke Azita Sadeghpour Gundula Povysil Marie T. McDonald Sheila Unger Susanne Greber‐Platzer Kristen L. Deak Nicholas Katsanis Andrea Superti‐Furga Kazuyuki Sugahara Erica E. Davis Shuhei Yamada Julia Vodopiutz 《Human mutation》2020,41(3):655-667
Congenital disorders of glycosylation (CDGs) comprise a large number of inherited metabolic defects that affect the biosynthesis and attachment of glycans. CDGs manifest as a broad spectrum of disease, most often including neurodevelopmental and skeletal abnormalities and skin laxity. Two patients with biallelic CSGALNACT1 variants and a mild skeletal dysplasia have been described previously. We investigated two unrelated patients presenting with short stature with advanced bone age, facial dysmorphism, and mild language delay, in whom trio‐exome sequencing identified novel biallelic CSGALNACT1 variants: compound heterozygosity for c.1294G>T (p.Asp432Tyr) and the deletion of exon 4 that includes the start codon in one patient, and homozygosity for c.791A>G (p.Asn264Ser) in the other patient. CSGALNACT1 encodes CSGalNAcT‐1, a key enzyme in the biosynthesis of sulfated glycosaminoglycans chondroitin and dermatan sulfate. Biochemical studies demonstrated significantly reduced CSGalNAcT‐1 activity of the novel missense variants, as reported previously for the p.Pro384Arg variant. Altered levels of chondroitin, dermatan, and heparan sulfate moieties were observed in patients’ fibroblasts compared to controls. Our data indicate that biallelic loss‐of‐function mutations in CSGALNACT1 disturb glycosaminoglycan synthesis and cause a mild skeletal dysplasia with advanced bone age, CSGALNACT1‐CDG. 相似文献
220.
Erika Ishihara Yuya Nagaoka Toshiaki Okuno Satoshi Kofuji Mari Ishigami‐Yuasa Hiroyuki Kagechika Kenya Kamimura Shuji Terai Takehiko Yokomizo Yukihiko Sugimoto Yasuyuki Fujita Akira Suzuki Hiroshi Nishina 《Genes to cells : devoted to molecular & cellular mechanisms》2020,25(3):197-214
Cell competition is a biological process by which unfit cells are eliminated from “cell society.” We previously showed that cultured mammalian epithelial Madin‐Darby canine kidney (MDCK) cells expressing constitutively active YAP were eliminated by apical extrusion when surrounded by “normal” MDCK cells. However, the molecular mechanism underlying the elimination of active YAP‐expressing cells was unknown. Here, we used high‐throughput chemical compound screening to identify cyclooxygenase‐2 (COX‐2) as a key molecule triggering cell competition. Our work shows that COX‐2‐mediated PGE2 secretion engages its receptor EP2 on abnormal and nearby normal cells. This engagement of EP2 triggers downstream signaling via an adenylyl cyclase‐cyclic AMP‐PKA pathway that, in the presence of active YAP, induces E‐cadherin internalization leading to apical extrusion. Thus, COX‐2‐induced PGE2 appears a warning signal to both abnormal and surrounding normal cells to drive cell competition. 相似文献