Oral health of aged inpatients

Objectives: The proportion of the population aged 65 years and over in Australia is expected to increase substantially, and more people in this age group are retaining their teeth and will require dental care. The objective of this study was to assess the oral health status of inpatients over the age of 65. Methods: Dental examinations were performed on inpatients at Fremantle Hospital. Standardised assessment forms were used to investigate factors related to medical history, hospital admission and oral health needs. Results: A total of 104 persons were examined, 56% were dentate. The dentate participants had an average decayed, missing and filled (DMF)‐index of 21.6 (SD 7.1). All edentulous participants had dentures and the estimated average age of their dentures was 18.1 years. Although the majority of participants (70.2%) were satisfied with their oral health status, 76.6% were professionally assessed to be in need of immediate dental care. Among the edentulous participants, 47.1% needed new dentures. Among all the participants, 75.3% also needed improved oral hygiene. Conclusion: There is a need to fully assess availability, appropriateness and effectiveness of models of oral health care delivery among the older population. This study clearly indicates a current problem and high levels of unmet need. With an increasing ageing population and higher retention levels of natural dentitions, this will result in higher levels of oral disease and need for prevention and care.     

Electrical impedance myography as a biomarker to assess ALS progression

Abstract Electrical impedance myography (EIM), a non-invasive, electrophysiological technique, has preliminarily shown value as an ALS biomarker. Here we perform a multicenter study to further assess EIM's potential for tracking ALS. ALS patients were enrolled across eight sites. Each subject underwent EIM, handheld dynamometry (HHD), and the ALS Functional Rating Scale-revised (ALSFRS-R) regularly. Techniques were compared by assessing the coefficient of variation (CoV) in the rate of decline and each technique's correlation to survival. Results showed that in the 60 patients followed for one year, EIM phase measured from the most rapidly progressing muscle in each patient had a CoV in the rate of decline of 0.62, compared to HHD (0.82) and the ALSFRS-R (0.74). Restricting the measurements to the first six months gave a CoV of 0.55 for EIM, 0.93 for HHD, and 0.84 for ALSFRS-R. For both time-periods, all three measures correlated with survival. Based on these data, a six-month clinical trial designed to detect a 20% treatment effect with 80% power using EIM would require only 95 patients/arm compared to the ALSFRS-R, which would require 220 subjects/arm. In conclusion, EIM can serve as a useful ALS biomarker that offers the prospect of greatly accelerating phase 2 clinical trials.     

Genetic Diversity of Asian Sea Bass,Lates calcarifer (Bloch) Populations in India Revealed by Randomly Amplified Polymorphic DNA

The genetic diversity of five feral populations of Asian Sea bass, Lates calcarifer collected from five isolated locations in India viz., Paradeep, Chilka lake, Kakinada, Chennai and Mumbai was studied using randomly amplified polymorphic DNA. Out of 20 primers screened, 5 decamer random primers amplified a total of 373 DNA bands of which 137 bands were polymorphic. The Kakinada population showed the highest polymorphism (65.71 %) whereas the Chennai population showed the lowest (48.28 %). The inter-population genetic similarity (GS) values estimated for the five populations were checked by one-way ANOVA and found to be significantly different at P < 0.01. The highest inter-population GS was found between Paradeep and Chilka (0.909) and lowest was between Kakinada and Chennai (0.800). Genetic distance tree developed by Unweighted Pair Group Method with Arithmetic Mean cluster analysis depicted that the Sea Bass of Paradeep, Chilka and Kakinada belong to one cluster while Chennai and Mumbai populations make different cluster. Overall, Randomly Amplified Polymorphic DNA technique could be used for genetic differentiation of diverse populations of Sea bass to provide information on their population genetic structure.     

New techniques for the reconstruction of complex vascular anatomies from MRI images.

The accurate representation of two-dimensional images in three dimensions has become important for many medical imaging applications and for cardiac magnetic resonance imaging (MRI) in particular. Reconstruction methods applied after data acquisition can produce three-dimensional information from two-dimensional data and make applications such as surgical planning more effective. Current reconstruction techniques usually demand contrast agents, and can suffer due to poor segmentation and sampling constraints that cause surface irregularities and distort dimensions. The novel technique presented here for anatomical modeling uses adaptive control grid interpolation (ACGI) to approximate data not captured by scanning, and a progressive shape-element segmentation technique to complete reconstruction. Quantitative validations conducted on models of pediatric cardiac malformations have confirmed the theoretical advantages of this technique, and that higher quality is achieved than with competing methods based on geometric parameters. Vascular diameters from reconstructions showed errors of less than 1% for a known geometry as compared to over 9% for competing methods. Qualitatively, models produced with the new methodology displayed substantial improvement over alternatives. Approximately 50 rare cardiac structures, including surgically altered Fontan and atypical aortic anatomies, have been reconstructed. All data used to create these reconstructions were acquired using standard pulse sequences and without contrast agents. Benefits of the new technique are particularly evident when complex vascular configurations complicate reconstruction. The proposed methodology enables a powerful tool allowing physicians to analyze and manipulate highly accurate and clearly presented vascular structures in an interactive medium.     

The learning curve for EUS-guided FNA of pancreatic cancer

BACKGROUND: EUS-guided FNA is an effective and safe method for tissue diagnosis of pancreatic cancer. However, EUS-guided FNA is technically challenging and requires special training. The number of cases required to become proficient and the technical steps required to achieve proficiency are unknown. METHODS: The first 57 EUS-guided FNAs of pancreatic masses by a trained endosonographer were analyzed retrospectively. For 50 masses, the histopathologic diagnosis ultimately was cancer. The sensitivity for the EUS-guided FNA diagnosis of adenocarcinoma was compared in quintiles of 10 procedures. RESULTS: Sensitivity for the diagnosis of pancreatic cancer from first to last quintile were, respectively, 50%, 40%, 70%, 90%, and 80%. There was a significant increase in sensitivity for the first 30 cases after improvement in specific technical skills: shortening of echoendoscope position, scrupulous maintenance of the US view of the needle tip at all times, swift jabbing punctures, sampling multiple areas of the mass in each pass, and performing more than 10 "jiggles" per needle pass. Sensitivity for the diagnosis of pancreatic cancer was greater than 80% for the last 20 of the 57 cases, a level that was maintained for cases 51 through 80. CONCLUSIONS: The current American Society for Gastrointestinal Endoscopy guideline of 25 supervised EUS-FNA procedures for the diagnosis of pancreatic adenocarcinoma is reasonable.     

Causes and Types of Precocious Puberty in North-West Iran

Objective

Precocious puberty is of concern because of the underlying disorders, the short adult stature, and the psychosocial difficulties. This study was carried out in order to evaluate the characteristics of children referred to pediatric endocrinology clinic with diagnosis of precocious puberty.

Methods

In a cross-sectional study between February 2007 and September 2009, all of the children referred to pediatric endocrinology clinic in North-West Iran with diagnosis of precocious puberty were recruited.

Findings

Data of 106 girls (82.2%) and 23 boys (17.8%) were analyzed. Mean age of the patients at the time of referral was 6.6±2.8 years (ranging 0.3-14 yr), which was 7±3.9 (ranging 0.3-14 yr) for boys and 6.6±2.5 (ranging 0.8-12 yr) for girls (P=0.6). Out of 129 subjects, 56(43.4%) had precocious puberty, 71.4% (35 cases) of them were due to central precocious puberty and 28.6% (16 cases) were pseudo-precocious puberty. 73 out of 129 subjects (56.6%) were due to normal variants of puberty, normal puberty, and no puberty. 87.5% of subjects with central precocious puberty were idiopathic.

Conclusion

Most of children referred with diagnosis of precocious puberty have benign normal variants. Most of cases with precocious puberty are affected with central precocious puberty, especially with idiopathic form of it.