Validation of DIAGNOdent for quantification of smooth-surface caries: an in vitro study

The aims were 1) to validate a laser-based device, KaVo DIAGNOdent, for quantification of caries lesions on smooth surfaces, using histopathologic and microradiographic analyses as the gold standard, and 2) to test inter- and intra-observer agreements. We also investigated the influence on DIAGNOdent readings of the storage medium used for extracted teeth. Two observers measured independently the tooth surfaces of 40 extracted premolars that had been stored in thymol-saturated saline. After subsequent storage in neutral-buffered formalin for 14 days they were re-measured. The teeth were then sectioned for histopathologic and microradiographic analysis. The Spearman rank correlation coefficients between lesion depth and DIAGNOdent readings were 0.78-0.83 and 0.85 for teeth stored in thymol-saturated saline and formalin, respectively. Inter- and intra-observer agreements were 0.94 and 0.95 when the teeth were stored in thymol-saturated saline. The DIAGNOdent reading was almost 1.5 times higher for teeth stored in formalin than for those stored in thymol saline. The Spearman rank correlation coefficient between mineral loss in enamel (deltaZ) and DIAGNOdent readings ranged from 0.64 to 0.68. It was concluded that DIAGNOdent may be helpful for assessing smooth-surface caries, but the cut-off points need to be assessed under clinical conditions. The increase in fluorescence associated with storage of teeth in formalin warrants further investigation.     

颌面部巨大血管畸形的放射治疗

目的 分析颌面部巨大血管畸形的临床特点及治疗方法,探讨放疗在血管畸形治疗中的可行性.方法 回顾分析2000年1月至2008年1月浙江大学医学院附属第二医院施行三维立体定向适形放射治疗的颌面部巨大血管畸形6例的临床资料,并主要根据影像检查与临床体检结果判断疗效.结果 6例患者在术中、术后无严重并发症,均对治疗后面部外形满意,MRI示病变范围缩小,甚至消失.随访0.5～9年,6例患者均无明显复发迹象.无一例出现视器损伤、张口受限等放疗并发症.结论 三维立体定向适形放疗可以作为一种安全有效治疗颌面部巨大血管畸形的方法.

Abstract：

Objective To discuss the clinical use of radiotherapy in the treatment of giant vascular malformation. Methods Six patients with giant vascular malformation in oral and maxillofacial region were treated by three dimensional radiation therapy in Department of Stomatology, The Second Affiliated Hospital,School of Medicne, Zhejiang University in the last ten years and the cilinical data were reviewed. The treatment results were evaluated by clinical examination and radiology. Results No complication was observed during and after the radiotherapy. All patients were satisfied with the aesthetic results. The lesions in MRI were all reduced and even disappeared. There was no sign of recurrence during the follow-up period.Conclusions Three dimensional radiotherapy is safe and effective for oral and maxillofical vascular malformation.     

溶菌酶抑菌作用的实验研究

溶菌酶（Ｌｙｓｏｚｙｍｅ，ＬＺＭ）抑菌作用实验证实溶菌酶对溶壁小球菌具有溶菌作用，并证实１２烷基磺酸钠存在时，溶菌酶对变形链球菌表现出不溶菌现象，这与表面活性剂１２烷基磺酸钠是溶菌酶的抑制剂相符合     

义齿塑料基托热压灌注成型新工艺—颜色稳定性的比较研究

目的：分析比较成型后的基托塑料可能发生的变色。方法：对不同粉、液比例灌注成型的基托塑料和常规模压成型的基托塑料，用分光测色仪观察颜色稳定性。结果：粉、液以２∶１体积比灌注成型的义齿基托，表面平整光滑，色调、饱和度与常规模压成型的义齿基托相同，而其表面可抛光性优于常规法。结论：２种方法成型的义齿基托对紫外线的敏感性无显著性差异。     

The design of a two-part acid-etched resin-bonded fixed partial denture.

A new framework design is presented for resin-bonded, acid-etched, fixed partial dentures. This innovative design divided the restoration into two parts, each with a separate path of insertion. The preparation of abutment teeth required minimal reduction and the pontic could not be displaced during function. Thirty-five patients with missing posterior teeth have been treated with this imaginative approach and after approximately 2 years, there have been no debonding failures.     

人牙周膜细胞中内源性骨形成蛋白的流式细胞仪分析

目的：定量检测和分析人牙周膜细胞（ＰＤＬＣ）表达内源性骨形成蛋白（ＢＭＰ）的情况。方法：应用ＢＭＰ单克隆抗体，通过流式细胞仪和免疫组化ＡＢＣ的方法双重判定。结果：在离体培养的人ＰＤＬＣ中有一半左右的细胞能表达ＢＭＰ。结论：牙周膜细胞具有一定的合成和分泌ＢＭＰ的能力；可以进一步认为人的牙周膜细胞是具有成骨潜能的，在牙周组织再生中有积极作用。     

器官培养中骨吸收因子的相互作用

为了研究不同浓度脂多糖（ｌｉｐｏｐｏｌｙｓａｃｃｈａｒｉｄｅ，ＬＰＳ）、脂磷壁酸（ｌｉｐｏｔｅｉｃｈｏｉｃａｃｉｄ，ＬＴＡ）、肿瘤坏死因子（ｔｕｍｏｕｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ，ＴＮＦ）及前列腺素Ｅ（ｐｒｏｓｔａｇｌａｎｄｉｎＥ，ＰＧＥ）的骨吸收作用和相互作用，采用４５Ｃａ标记的１９天胚胎鼠长骨干器官培养，用样品道比法定量分析。结果表明，上述因子均能在器官培养水平刺激骨吸收，与对照组相比差异有显著性（Ｐ＜０．０５）。低浓度的ＬＰＳ与ＴＮＦ、ＬＰＳ与ＰＧＥ、ＴＮＦ与ＰＧＥ相互作用，超过各因子单独作用之和（期望值）；ＬＰＳ与ＬＴＡ、ＴＮＦ与ＬＴＡ相互作用，等于期望值；而ＬＴＡ与ＰＧＥ相互作用，小于期望值。研究结果提示，多种骨吸收因子的相互作用在牙周病骨吸收发病机理中有重要意义。     

Role of type IV collagene and type IV collagenase in the invasion and metastasis of salivary adenoid cystic carcinoma]

Using immunohistochemical ABC method, the authors studied the distribution of type IV collagen and type IV collagenase in 25 cases ACC. The result showed type IV collagen was found in vascular and epithelial basement membrance, and in partly cyst of sieve-like and tubular pattern, and in cytoplasm of tumor cell. Type IV collagenase was detected in membrance and cytoplasm of tumor cell. Expression of type IV collagen and type IV collagenase were correlated with pathologic type, TNM stage and metastasis. Much positive staining of type IV collagen was found in sieve-tubular pattern, early stage of TNM and without metastasis, but much positive staining of type IV collagenase was found in solid pattern, later stage of TNM and metastasis. The result suggested that the loss of type IV collagen and over-expression of type IV collagenase may be markers of malignance of ACC.     

牙龈卟啉菌荚膜的热敏感性

以细胞计数法观察了牙龈卟啉菌荚膜的热敏感性，结果显示：牙龈卟啉菌荚膜在５０℃下，水浴３０ｍｉｎ，活性基本不受影响，６５℃水浴３０ｍｉｎ半量活性成份丧失，８０℃水浴３０ｍｉｎ活性完全丧失，说明牙龈卟啉菌荚膜是一热不稳定性物质。