Systemic BMSC homing in the regeneration of pulp-like tissue and the enhancing effect of stromal cell-derived factor-1 on BMSC homing

Pulp regeneration caused by endogenous cells homing has become the new research spot in endodontics. However, the source of functional cells that are involved in and contributed to the reconstituting process has not been identified. In this study, the possible role of systemical BMSC in pulp regeneration and the effect of stromal cell-derived factor-1 (SDF-1) on stem cell recruitment and angiogenesis were evaluated. 54 mice were divided into three groups: SDF-1 group (subcutaneous pockets containing roots with SDF-1 absorbed neutralized collagen gel and the green fluorescent protein (GFP) positive BMSCs transplantation via the tail vein), SDF-1-free group (pockets containing roots with gel alone and GFP + BMSCs transplantation) and Control group (pockets containing roots with gel alone). The animals were sacrificed after the roots were implanted into subcutaneous pockets for 3 weeks. Histomorphometric analysis was performed to evaluate the regenerated tissue in the canal by hematoxylin and eosin (HE) staining. The homing of the transplanted BMSCs was monitored with a fluorescence microscope and immunohistochemical analysis. The expression of ALP in new formed tissue was detected immunohistochemically. Dental-pulp-like tissue and new vessels were regenerated and GFP-positive BMSCs and expression of ALP could be observed in both SDF-1 group and SDF-1-free group. Furthermore, more GFP+ cells, stronger expression of ALP and stronger angiogenesis were found in the SDF-1 group than in the SDF-1-free group. To conclude, systemic BMSC can home to the root canal and participate in dental-pulp-like tissue regeneration. Intracanal application of SDF-1 may enhance BMSC homing efficiency and angiogenesis.     

Tumor-stroma ratio is an independent predictor for survival in NSCLC

Tumor-stroma ratio (TSR) has been identified as a new and practicable prognostic factor in some solid tumors. The aim of the study is to evaluate the prognostic value of TSR in non-small cell lung cancer (NSCLC). A total of 404 patients who underwent surgery resection for NSCLC were included in this study. TSR was assessed visually on the hematoxylin-stained tissue sections of surgical specimens. Patients with more than 50% intratumor stroma were quantified as the stroma-rich group and those with less than 50% as the stroma-poor group. In 404 cases of tissue samples, 302 cases were included in the stroma-poor group, while 102 cases in stroma-rich group. The different expression of TSR in NSCLC tissue was not correlated with gender, age, smoking history, tumor diameter, histology, differentiation grade and pTNM staging. In the Cox univariate and multivariate analyses of the 5-year OS, the HRs of the TSR were 1.818 (95% CI; 1.323-2.497; P<0.001) and 1.748 (95% CI; 1.262-2.422; P<0.05), respectively. As for DFS, the HRs were 1.715 (95% CI; 1.249-2.354; P<0.001) and 1.570 (95% CI; 1.135-2.172; P<0.05). Stroma-rich tumors were associated with poor prognosis and an increased risk of relapse, which may serve as a new prognostic histological characteristic in NSCLC.     

3种实验性牙本质粘接处理剂保存期的对比研究

目的:观察保存时间对3种实验性牙本质粘接处理剂的粘接强度的影响。方法:将3种实验性粘接处理剂10-甲基丙烯酰氧癸基双氢磷酸盐-2-羟乙基丙烯酸酯(MDP-HEMA),MDP-N-甲基丙烯酰甘氨酸(MDP-NMGly),N-甲基丙烯酰-2-氨乙基膦酸-NMGly(NMEP-NMGly)制备后置于40℃保温箱中6周和14周,在不同的保存时期分别测试剪切粘接强度(n=14)和p H值以及断裂模式和扫面电镜观察。结果:经过14周40℃的保存,MDP-HEMA剪切粘接强度由(17.61±1.56)MPa下降到(7.53±1.76)MPa;而MDP-NMGly和NMEP-NMGly则没有显著下降。断裂模式显示经过14周保存的MDP-HEMA和MDP-NMGly以界面破坏为主;而NMEP-NMGly仍然以树脂/牙本质断裂为主。SEM观察保存14周后MDP-HEMA和MDP-NMGly均有树脂与牙本质的分离,而NMEP-NMGly则没有明显的界面分离。结论:NMEP-NMGly具有更好的稳定性。     

早期穴位按摩配合电动吸乳促进母乳喂养的效果研究

目的 探讨早期穴位按摩配合电动吸乳对母乳喂养的影响.方法 选择100例产妇 ,随机分为观察组(50例)和对照组(50例) ,对照组采用常规乳房护理 ,观察组在此基础上进行早期穴位按摩配合电动吸乳.记录两组产妇泌乳始动时间 ;产后24h、48h、72h的泌乳量 ;产后2d、3d、4d的乳房肿胀硬度以及产后第5天纯母乳喂养率的情况.结果 观察组产妇泌乳始动时间早于对照组( P<0 .05 );观察组产后24 h、48 h、72 h的泌乳量均多于对照组(P<0 .05);观察组产后第5天纯母乳喂养率明显高于对照组(P<0 .05).结论 基于常规乳房护理的早期穴位按摩配合电动吸乳可增加乳汁分泌 ,促进母乳喂养成功.     

错误相关负电位在精神科临床上推广——附50例正常成人分析

目的:应用引进的德国仪器及新技术探讨并建立健康成人错误相关负电位(ERN)的正常值。方法:应用德国Brain Products公司的ERP记录与分析系统,对50例21~55岁健康成人右利手受试者,作了ERN检测。结果:(1)32个头皮记录点皆在约100~150ms处出现了一个负波,健康成人ERN波形较稳定。(2)在Cz、Fz、Pz、C3、C4 5个脑区,建立了健康成人ERN潜伏期和波幅均值。(3)左右侧比较及男女性别比较差异无统计学意义(P>0.05)。结论:健康成人ERN较稳定和可靠。ERN可作为一个有临床推广应用价值的ERP而用于神经精神科临床。     

Inhibitory effect of midkine-binding peptide on tumor proliferation and migration

Background: To investigate the inhibitory effect of midkine-binding peptides on human umbilical vein endothelial cells (HUVECs) proliferation and angiogenesis of xenograft tumor. Methods: The midkine-binding peptides were panned by Ph.D.-7^™ Phage Display Peptide Library Kit, and the specific binding activities of positive clones to target protein were examined by phage ELISA. The effect of midkine-binding peptides on proliferation of HUVECs was confirmed by MTT test. The xenograft tumor model was formed in BALB/c mice with the murine hepatocarcinoma cells H22 (H22). Microvessel density (MVD) was analyzed by immunohistochemistry of factor VIII staining. Results: Midkine-binding peptides have the inhibitory effects on tumor angiogenesis, a proliferation assay using human umbilical vein endothelial cells (HUVECs) indicated that particular midkine-binding peptides significantly inhibited the proliferation of the HUVECs. Midkine-binding peptides were also observed to efficiently suppress angiogenesis induced by murine hepatocarcinoma H22 cells in BALB/c nude mice. Conclusion: The midkine-binding peptides can inhibit solid tumor growth by retarding the formation of new blood vessels. The results indicate that midkine-binding peptides may represent potent anti-angiogenesis agents in vivo.     

Expression of cancer stem cell markers and their correlation with pathogenesis in vascular tumors

Objective: Vascular tumor, which belongs to a kind of complicated lesion in soft tissue tumor, is derived from mesenchymal tissue. Although many studies have been focused on the pathogenesis of vascular tumors in human, the specific mechanism of the vascular tumors was currently unclear. Previous studies have reported an association of cancer stem cells with the development of tumor in many solid tumors. Thus the purpose of this study was to explore whether different expression level of cancer stem cell markers including CD29, CD44, CD133, nestin and ALDH1 in vascular tumor may help to elucidate the possible pathogenesis of vascular tumor. In present study, tissues of 9 cases of hemangioma, 22 cases of hemangiosarcoma, 3 cases of Kaposi’s sarcoma, and 5 cases of hemangioendothelioma were immunostained for CD29, CD44, CD133, nestin and ALDH1. Of the 39 vascular tumor cases included in the current study, CD29, CD133 and nestin were positive in most vascular tumor cases. Although CD44 and ALDH1 were observed in vascular tumor cases, the percentage of cells staining for the two markers was less than 2% in all cases of vascular tumor. Capillary hemangiomas exhibited significantly higher expression rate of CD29 and nestin compared with malignant vascular tumors and hemangioendotheliomas (P<0.05, Fisher’s exact test), while CD44, CD133 and ALDH1 exhibited no statistically significant difference between these two groups. Pearson correlation analysis exhibited that CD29 expression and nestin expression in vascular tumor were no statistically significant relationship (C=0.288, P=0.063>0.05). Our findings confirmed that the five cancer stem cells markers, including CD29, CD44, CD133, nestin and ALDH1, exhibited different expression levels in vascular tumors and demonstrated that immonhistochemical analysis for cancer stem cells markers may provide useful information for studying the pathogenesis of vascular tumors.