全文获取类型
收费全文 | 1588篇 |
免费 | 69篇 |
国内免费 | 30篇 |
专业分类
耳鼻咽喉 | 15篇 |
儿科学 | 52篇 |
妇产科学 | 9篇 |
基础医学 | 251篇 |
口腔科学 | 34篇 |
临床医学 | 105篇 |
内科学 | 293篇 |
皮肤病学 | 4篇 |
神经病学 | 116篇 |
特种医学 | 336篇 |
外科学 | 87篇 |
综合类 | 32篇 |
预防医学 | 72篇 |
眼科学 | 8篇 |
药学 | 104篇 |
中国医学 | 6篇 |
肿瘤学 | 163篇 |
出版年
2022年 | 6篇 |
2021年 | 17篇 |
2020年 | 10篇 |
2019年 | 10篇 |
2018年 | 15篇 |
2017年 | 7篇 |
2016年 | 12篇 |
2015年 | 20篇 |
2014年 | 23篇 |
2013年 | 22篇 |
2012年 | 38篇 |
2011年 | 61篇 |
2010年 | 22篇 |
2009年 | 35篇 |
2008年 | 64篇 |
2007年 | 73篇 |
2006年 | 70篇 |
2005年 | 80篇 |
2004年 | 81篇 |
2003年 | 50篇 |
2002年 | 67篇 |
2001年 | 57篇 |
2000年 | 31篇 |
1999年 | 38篇 |
1998年 | 59篇 |
1997年 | 49篇 |
1996年 | 71篇 |
1995年 | 60篇 |
1994年 | 36篇 |
1993年 | 43篇 |
1992年 | 24篇 |
1991年 | 29篇 |
1990年 | 30篇 |
1989年 | 40篇 |
1988年 | 42篇 |
1987年 | 30篇 |
1986年 | 28篇 |
1985年 | 39篇 |
1984年 | 24篇 |
1983年 | 24篇 |
1982年 | 27篇 |
1981年 | 25篇 |
1980年 | 14篇 |
1979年 | 7篇 |
1978年 | 8篇 |
1977年 | 27篇 |
1976年 | 19篇 |
1975年 | 15篇 |
1969年 | 2篇 |
1875年 | 2篇 |
排序方式: 共有1687条查询结果,搜索用时 0 毫秒
51.
Chioza B Osei-Lah A Nashef L Suarez-Merino B Wilkie H Sham P Knight J Asherson P Makoff AJ 《European journal of human genetics : EJHG》2002,10(12):857-864
Idiopathic generalised epilepsy (IGE) is a common form of epilepsy, including several defined and overlapping syndromes, and likely to be due to the combined actions of mutations in several genes. In a recent study we investigated the calcium channel gene CACNA1A for involvement in IGE, unselected for syndrome, by means of association studies using several polymorphisms within the gene. We reported a highly significant case/control association with a silent single nucleotide polymorphism (SNP) in exon 8 that we confirmed by within-family analyses. In this present study we screened the gene for novel SNPs within 25 kb of exon 8, which have enabled us to define the critical region of CACNA1A in predisposing to IGE. Several intronic SNPs were identified and three, within 1.5 kb of exon 8 and in strong linkage disequilibrium with each other and with the original SNP, were significantly associated with IGE (P=0.00029, P=0.0015 and P=0.010). The associations were not limited to an IGE syndrome or other subgroup. Another SNP, 25 kb away, in intron 6 was also significantly associated with IGE (P=0.0057) but is not in linkage disequilibrium with the SNPs around exon 8. Haplotype predictions revealed even more significant associations (3-marker haplotype: P<10(-6)). Logistic regression showed that all the data can be explained by two of the SNPs, which is consistent with two functionally significant variants being responsible for all five associations, although a single variant cannot be excluded. The functionally significant variant(s) are unlikely to be exonic and suggests an effect on expression or alternative splicing. 相似文献
52.
Sham JS Tang TC Fang Y Sun L Qin LX Wu QL Xie D Guan XY 《Cancer Genetics and Cytogenetics》2002,133(1):39-44
Ovarian cancer is one of the most frequent gynecological malignancies worldwide with a poor prognosis. Comparative genomic hybridization has been applied to detect recurrent chromosome alterations in 31 primary ovarian carcinomas in Chinese women. Several nonrandom chromosomal changes were identified including gains of 3q (17 cases, 55%) with a minimum region at 3q25 through q26, 8q (16 cases, 52%), 19q (12 cases, 39%), Xq (11 cases, 35%), 1q (10 cases, 32%), 12p12 through q13 (10 cases, 32%), 17q (10 cases, 32%) with a minimum gain region at 17q21, and 20q (9 cases, 29%); and losses of 16q (9 cases, 29%), 1p (7 cases, 23%), 18q (7 cases, 23%), and 22 (7 cases, 23%). High-copy-number amplification was detected in eleven cases. Amplification of 3q25 through q26 was detected in four cases, and amplifications of 8q24 and 12p11.2 through q12 were observed in three cases each. The recurrent gains and losses of chromosomal regions identified in this study provide candidate regions that may contain oncogenes or tumor suppressor genes involved in the development and progression of ovarian cancer. 相似文献
53.
Brain volumes in familial and non-familial schizophrenic probands and their unaffected relatives 总被引:5,自引:0,他引:5
McDonald C Grech A Toulopoulou T Schulze K Chapple B Sham P Walshe M Sharma T Sigmundsson T Chitnis X Murray RM 《American journal of medical genetics》2002,114(6):616-625
Structural brain abnormalities are consistently reported in schizophrenic subjects but the etiology of these abnormalities remains unclear. We tested the contribution of genetic predisposition and obstetric complications to the structural brain abnormalities found in schizophrenic probands and their relatives. MRI scans were carried out on 35 schizophrenic probands from families multiply affected with the disorder, and 63 of their unaffected relatives, including 10 parents who appeared to transmit genetic risk to their children; as well as 31 schizophrenic probands from families with no other affected members, 33 of their unaffected relatives; and finally 68 controls. Volumetric measurements of whole brain, lateral ventricles, third ventricle, cerebellum, and temporal lobes were completed for each subject. The impact of obstetric complications on brain structure was assessed across the gradient of presumed genetic predisposition. Both groups of schizophrenic probands displayed enlargement of the lateral and third ventricles, and there was a gradient of ventricular enlargement amongst the unaffected relatives in proportion to their likelihood of carrying schizophrenic genes. Ventricular enlargement was largely confined to males in both probands and unaffected relatives. Obstetric complications were associated with ventricular enlargement only in the familial probands. Non-familial probands displayed reduced volume of the temporal lobes bilaterally. In families with several schizophrenic members, ventricular enlargement is a marker for genetic liability, particularly in males. Individuals inheriting the susceptibility to schizophrenia appear particularly prone to develop ventricular enlargement in response to obstetric complications. 相似文献
54.
Establishment and characterization of human metastatic hepatocellular carcinoma cell line 总被引:6,自引:0,他引:6
Wen JM Huang JF Hu L Wang WS Zhang M Sham JS Xu JM Zeng WF Xie D Liang LJ Guan XY 《Cancer Genetics and Cytogenetics》2002,135(1):91-95
Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide with a poor prognosis. Recently, we established a HCC cell line from a metastatic HCC tumor. GTG banding analysis was performed and the karyotype showed that this metastatic HCC cell line is a hypertriploid (71-78 chromosomes) with a large marker chromosome containing a long homogeneously staining region (hsr). Comparative genomic hybridization was applied to characterize the chromosomal alterations in this metastatic HCC cell line. The results showed that the hsr was composed of amplified DNA sequences from 11q13. Further characterization of the hsr may lead to the isolation of the putative amplified oncogene at 11q13. 相似文献
55.
Sarah Curran Shaun Purcell Ian Craig Philip Asherson Pak Sham 《American journal of medical genetics. Part B, Neuropsychiatric genetics》2005,(1):42-47
Molecular studies of attention deficit hyperactivity disorder (ADHD) have identified susceptibility genes for the categorically diagnosed disorder using operational diagnostic criteria. Here, we take a QTL approach to mapping genes for ADHD using a composite continuous index of ADHD behavior in a large epidemiological sample. Previous studies of clinical ADHD suggest that two functional polymorphisms in the serotonin transporter gene (SLC6A4), one in the 5'-regulatory region of the gene (5-HTTLPR) and the other a VNTR (5-HTTVNTR) in the second intron, as well as a single nucleotide polymorphism in the 3'-untranslated region (3'-UTR SNP), may be associated with the disorder. Here, we investigate these polymorphisms as well as an additional ten SNPs spread across the gene. We found significant association with the long (L) allele of the 5-HTTLPR; P = 0.019, but neither the 5-HTTVNTR nor the 3'-UTR SNP were significantly associated. Significant associations (P < 0.05) were found for a further 5 the 10 other markers tested. We found evidence for two haplotype blocks spanning the region. We found strong evidence for association with the first haplotype block (comprised of four markers), with the significance of a combined primary and secondary test of association reaching an empirical P value = 0.0054 for the global test and an empirical P value = 0.00081 for the largest local test. Thus, we show here that SLC6A4, which has a major influence on brain serotonin availability, may be a QTL for ADHD. 相似文献
56.
Correlation of AIB1 overexpression with advanced clinical stage of human colorectal carcinoma 总被引:7,自引:0,他引:7
AIB1, a member of the steroid receptor coactivator 1 family, has been cloned on 20q12 and is a candidate oncogene in human breast cancer. It is commonly amplified and overexpressed in several types of human cancers. In this study, we examined the expression of AIB1, as related to clinicopathologic features, in 85 human colorectal cancers (CRCs). The status of the number of AIB1 copies, p53 expression, and DNA ploidy was also analyzed. The overexpression of AIB1 was detected in 35% of CRCs. Amplification of AIB1 was observed in 10% of CRCs. In addition, the overexpression of AIB1 was observed more frequently in CRCs in later clinical stages (T3 N1 M0/T3 N0 2M1), compared with that in T3 N0 M0 stage (P < .05). These results suggest that overexpression of AIB1 might provide a selective advantage for the developmental growth and/or progression of subsets of CRCs. In addition, a significant correlation (P < .05) of overexpression of AIB1 with p53 overexpression as well as with aneuploid DNA content was observed in these CRCs. The overexpression of p53 was also correlated significantly with CRC DNA ploidy (P < .05). Furthermore, there was a substantial population of CRCs showing overexpression of both AIB1 and p53 protein and all had aneuploid DNA content; most of these were in the later clinical stage. These findings suggest a possible convergence of AIB1 with a pathway involving p53, which might induce chromosomal instability and affect the clinical phenotype of a subset of CRCs. 相似文献
57.
Yang J Yang W Hirankarn N Ye DQ Zhang Y Pan HF Mok CC Chan TM Wong RW Mok MY Lee KW Wong SN Leung AM Li XP Avihingsanon Y Rianthavorn P Deekajorndej T Suphapeetiporn K Shotelersuk V Baum L Kwan P Lee TL Ho MH Lee PP Wong WH Zeng S Zhang J Wong CM Ng IO Garcia-Barceló MM Cherny SS Tam PK Sham PC Lau CS Lau YL 《Human molecular genetics》2011,20(3):601-607
58.
Homozygosity mapping has played an important role in detecting recessive mutations using families of consanguineous marriages. However, detection of regions identical and homozygosity by descent (HBD) when family data are not available, or when relationships are unknown, is still a challenge. Making use of population data from high-density SNP genotyping may allow detection of regions HBD from recent common founders in singleton patients without genealogy information. We report a novel algorithm that detects such regions by estimating the population haplotype frequencies (HF) for an entire homozygous region. We also developed a simulation method to evaluate the probability of HBD and linkage to disease for a homozygous region by examining the best regions in unaffected controls from the host population. The method can be applied to diseases of Mendelian inheritance but can also be extended to complex diseases to detect rare founder mutations that affect a very small number of patients using either multiplex families or sporadic cases. Testing of the method on both real cases (singleton affected) and simulated data demonstrated its superb sensitivity and robustness under genetic heterogeneity. 相似文献
59.
Recurrent genetic alterations in 26 colorectal carcinomas and 21 adenomas from Chinese patients 总被引:5,自引:0,他引:5
He QJ Zeng WF Sham JS Xie D Yang XW Lin HL Zhan WH Lin F Zeng SD Nie D Ma LF Li CJ Lu S Guan XY 《Cancer Genetics and Cytogenetics》2003,144(2):112-118
Colorectal cancer (CRC) is one of the most common malignancies worldwide. The incidence of CRC in the Chinese population has increased dramatically during the last two decades; however, nonrandom chromosomal alterations in Chinese patients have not been described. In the present study, comparative genomic hybridization (CGH) was applied to detect recurrent chromosome alterations in 26 primary colorectal carcinomas and 21 colorectal adenomas from Chinese patients. In CRC, several recurrent chromosomal changes were found, including gains of 8q (14/26 cases, 54%), 20q (54%), 3q (50%), 13q (50%), 5p (46%), 7p (42%), 7q (42%), and 12p (38%) and losses of 18q (65%) and 17p (42%). From comparison with previous CGH studies, the frequent gains of 3q and 12p might be distinctive occurrences in Chinese patients. The distribution of frequently found chromosomal alterations in different locations was studied. The gain of 20q was more frequently found in colon cancer (P<0.01) and the gain of 12p was more frequently found in rectal cancer. Chromosomal alterations were found in 19/21 of adenomas; the most frequent chromosomal alteration was the loss of 18q (9/21 cases, 43%). These recurrent alterations provide several starting points for the isolation of candidate oncogenes and tumor suppressor genes. 相似文献
60.
102T/C polymorphism of serotonin receptor type 2A gene is not associated with schizophrenia in either Chinese or British populations 总被引:7,自引:0,他引:7
He L Li T Melville C Liu S Feng GY Gu NF Fox H Shaw D Breen G Liu X Sham P Brown J Collier D St Clair D 《American journal of medical genetics》1999,88(1):95-98
Several pieces of evidence implicate serotonin receptors in the aetiology of schizophrenia, and recently a number of studies have reported a genetic association between the 102T/C polymorphism of serotonin receptor type 2A gene and schizophrenia. Unfortunately a number of failures to replicate these positive associations in both Caucasian and Chinese populations have also been reported. We have examined the 102T/C polymorphism by PCR amplification and restriction analysis of DNA from: 202 schizophrenics and 202 controls from Shanghai; 112 schizophrenics and 224 parents from Chengdu, Cina; and 253 schizophrenics and 244 controls from the the UK. We find no evidence of association or transmission disequilibrium between the 102T/C polymorphism and schizophrenia in any of the groups we have examined. We conclude that either the original positive reports occurred by chance or any effect must be minimal, and urge caution in interpreting small positive results derived using data from different centres. 相似文献