Expression analysis of microRNA-155 in type 2 diabetes in Kashmiri population

International Journal of Diabetes in Developing Countries - The present study aimed to evaluate the expression of microRNA-155 (miR-155) in type 2 diabetes mellitus (T2DM) and assess its...     

Allelic loss on chromosomes 8p, 22q and 18q (DCC) in human prostate cancer

Previous studies have suggested the involvement of tumour-suppressor genes on chromosomes 8p, 22q and 18q (DCC) in prostate cancer. The aim of this study was to further characterize these regions. We investigated 20 polymorphic regions on the 3 chromosome arms in 43 cancers and 10 cases of benign prostatic hyperplasia (BPH). Allelic loss was observed in 72% of cancers on 8p, 16% on 22q and 24% at DCC. For BPH, loss was observed in 20% on 8p and in 12% at DCC. The low incidence of LOH on 22q implies that this locus has no significant role in prostate carcinogenesis. At DCC, although the overall incidence was low, tumours with LOH were mostly of high grade or had metastases, suggesting a role for this gene in prostate cancer progression. On chromosome 8p, 29% of cancers had deletions at the LPL locus on 8p22 and 60% had deletions within a region flanked by the markers D8S339 and ANKI on 8p11.1-p21.1. Within this region, 2 distinct areas of allelic loss were observed, at one or both ANKI and D8S255, and in the region defined by the markers D8S259-D8S505. For the regions 8p22 and ANKI-D8S255, tumours with metastases had a greater frequency of LOH compared to non-metastasizing tumours, suggesting the presence of putative metastasis-suppressor genes in these regions. © 1996 Wiley-Liss, Inc.     

Formulation of re-dispersible dry o/w emulsions using cellulose nanocrystals decorated with metal/metal oxide nanoparticles

This study describes for the first time the preparation of re-dispersible surfactant-free dry eicosane oil emulsion using cellulose nanocrystals (CNCs) using the freeze-drying technique. Surface properties of CNCs constitute a critical point for the stability of o/w emulsions and thus can affect both the droplet size and dispersion properties of the emulsion. Therefore, surface modification of CNCs was performed to understand its effect on the size of the obtained re-dispersible dry o/w eicosane emulsion. Decoration of the CNC surface with metal and metal oxide nanoparticles was conducted through the available alcoholic groups of glycosidic units of CNC, which played a dual role in reducing and stabilizing nanoparticles. Of these nanoparticles, silver (AgNPs), gold (AuNPs), copper oxide (CuO-NPs), and iron oxide (Fe₃O₄-NPs) nanoparticles were prepared via a facile route using alkali activated CNCs. Thorough characterizations pertaining to the as-prepared nanoparticles and their re-dispersible dry eicosane o/w emulsions were investigated using UV-vis spectroscopy, TEM, XRD, particle size, zeta potential, and STEM. Results confirmed the ability of CNCs to stabilize and/or reduce the formed nanoparticles with different sizes and shapes. These nanoparticles showed different shapes and surface charges accompanied by individual morphologies, reflecting on the stability of the re-dispersed dry eicosane emulsions with droplet sizes varying from 1.25 to 0.5 μm.

Schematic diagram for the detailed preparation of dry eicosane o/w emulsions.     

Multiple sclerosis: serial study of gadolinium-enhanced MR imaging

Thirteen patients with definite multiple sclerosis (MS), studied 16-24 months previously with magnetic resonance (MR) imaging with and without enhancement by intravenously administered gadolinium diethylenetriaminepentaacetic acid (DTPA) dimeglumine, were reexamined with a similar protocol. Assessment of enhancement and clinical activity in both studies revealed that enhancement was observed in 13 of 14 cases in which clinical activity had changed within 4 weeks of the study and thus appeared more sensitive than clinical examination in determining active disease. The 3-minute postinjection, short repetition time image (TR) was the most efficient for depicting enhancement. Enhancing lesions (active plaques) arose from previously hyper- or isointense regions on long TR images. Previously active lesions reverted to areas of iso- or hyperintensity on long TR images. Serial comparison of long TR images in this population reveals a decrease in high-intensity lesions on long TR images in some cases and an increase in others. The findings of high-intensity regions on long TR images and previously enhancing lesions both becoming isointense suggests that transient inflammatory changes with concomitant edema without demyelination and/or with significant remyelination may occur in some MS lesions. MS lesions are dynamic; both active and inactive lesions may show dramatic change on longitudinal MR imaging studies.     

Nonsentinel node metastasis in breast cancer patients: assessment of an existing and a new predictive nomogram

BACKGROUND: The accurate prediction of nonsentinel node (NSN) metastasis in breast cancer patients remains uncertain. METHODS: The medical records of 574 breast cancer patients from 2 different institutions (Mayo Clinic and University of Michigan) with sentinel lymph node biopsy examination and completion axillary lymph node dissection were reviewed for multiple clinicopathologic variables. The Memorial Sloan Kettering Cancer Center nomogram performance for prediction of NSN metastases was assessed. A new model was developed with clinically relevant variables and possible advantages. RESULTS: The Memorial Sloan Kettering Cancer Center nomogram predicted the likelihood of NSN metastasis with an area under the receiver operating characteristic curve of .72 and .86. For predicted probability cut-off points of 5% and 10%, the false-negative rates were 0% and 14% (Mayo), and 17% and 11% (Michigan). A new model was developed with similar area under the curve but lower false-negative rates for low-probability subgroups. CONCLUSIONS: Predictive models for NSN tumor burden are imperfect.     

Cellulose Nanofibrils as a Damping Material for the Production of Highly Crystalline Nanosized Zeolite Y via Ball Milling

Nanosized zeolite Y is used in various applications from catalysis in petroleum refining to nanofillers in water treatment membranes. Ball milling is a potential and fast technique to decrease the particle size of zeolite Y to the nano range. However, this technique is associated with a significant loss of crystallinity. Therefore, in this study, we investigate the effect of adding biodegradable and recyclable cellulose nanofibrils (CNFs) to zeolite Y in a wet ball milling approach. CNFs are added to shield the zeolite Y particles from harsh milling conditions due to their high surface area, mechanical strength, and water gel-like format. Different zeolite Y to CNFs ratios were studied and compared to optimize the ball milling process. The results showed that the optimal zeolite Y to CNFs ratio is 1:1 to produce a median particle size diameter of 100 nm and crystallinity index of 32%. The size reduction process provided accessibility to the zeolite pores and as a result increased their adsorption capacity. The adsorption capacity of ball-milled particles for methylene blue increased to 29.26 mg/g compared to 10.66 mg/g of the pristine Zeolite. These results demonstrate the potential of using CNF in protecting zeolite Y particles and possibly other micro particles during ball milling.     

Rising level of arsenic in water and fodder: a growing threat to livestock and human populations in Pakistan

Arsenic (As) is considered to be one of the top environmental contaminants of concern worldwide. In many regions in Pakistan, there are reports of increased levels of As in the environment, and this metalloid remains a major source of contamination of soil, water and fodder. This pathway of exposure has emerged as a new threat for both wildlife and the surrounding human communities because of the mobilization and accumulation of As via the food chain. The objective of this critical review is to synthesize historical information about As levels in the environmental resources of Pakistan to better characterize the risk to livestock and humans in the region. The South Punjab and Sindh are the dominant areas which are reported to contain the highest As contamination compared to other regions. This is a concern as millions of animals as well as the human population are facing significant health issues due to As. For example, some of the population of Sindh province are potentially faced with As levels ～10–50?μg/L in drinking water, which is well above safe drinking limits of 10?μg/L outlined by the World Health Organization. These collective data on As are anticipated to be a useful source of information for decisions by the environmental protection agency in Pakistan regarding the adoption of preventive measures against its adverse impacts and toxicity.