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161.
162.
Nerea Matamala Beatriz Lara Gema Gómez-Mariano Selene Martínez Irene Vázquez-Domínguez Álvaro Otero-Sobrino Antonio Muñoz-Callejas Elena Sánchez Cristina Esquinas Ana Bustamante Sergio Cadenas Sergio Curi Lourdes Lázaro María Teresa Martínez Esther Rodríguez Marc Miravitlles María Torres-Duran Inés Herrero Beatriz Martínez-Delgado 《Archivos de bronconeumologia》2021,57(7):457-463
IntroductionAlpha-1 antitrypsin deficiency (AATD) is a genetic condition resulting in lung and liver disease with a great clinical variability. MicroRNAs have been identified as disease modifiers; therefore miRNA deregulation could play an important role in disease heterogeneity. Members of miR-320 family are involved in regulating of multiple processes including inflammation, and have potential specific binding sites in the 3′UTR region of SERPINA1 gene. In this study we explore the involvement of miR-320c, a member of this family, in this disease.MethodsFirstly in vitro studies were carried out to demonstrate regulation of SERPINA1 gene by miR-320. Furthermore, the expression of miR-320c was analyzed in the blood of 98 individuals with different AAT serum levels by using quantitative PCR and expression was correlated to clinical parameters of the patients. Finally, HL60 cells were used to analyze induction of miR-320c in inflammatory conditions.ResultsOverexpression of miR-320 members in human HepG2 cells led to inhibition of SERPINA1 expression. Analysis of miR-320c expression in patient's samples revealed significantly increased expression of miR-320c in individuals with pulmonary disease. Additionally, HL60 cells treated with the pro-inflammatory factor lipopolysaccharide (LPS) showed increase in miR-320c expression, suggesting that miR-320c responds to inflammation.ConclusionOur findings demonstrate that miR-320c inhibits SERPINA1 expression in a hepatic cell line and its levels in blood are associated with lung disease in a cohort of patients with different AAT serum levels. These results suggest that miR-320c can play a role in AAT regulation and could be a biomarker of inflammatory processes in pulmonary diseases. 相似文献
163.
Dopamine modulation of GABAergic transmission in the prefrontal cortex (PFC) is thought to be critical for sustaining cognitive processes such as working memory and decision-making. Here, we developed a neurocomputational model of the PFC that includes physiological features of the facilitatory action of dopamine on fast-spiking interneurons to assess how a GABAergic dysregulation impacts on the prefrontal network stability and working memory. We found that a particular non-linear relationship between dopamine transmission and GABA function is required to enable input selectivity in the PFC for the formation and retention of working memory. Either degradation of the dopamine signal or the GABAergic function is sufficient to elicit hyperexcitability in pyramidal neurons and working memory impairments. The simulations also revealed an inverted U-shape relationship between working memory and dopamine, a function that is maintained even at high levels of GABA degradation. In fact, the working memory deficits resulting from reduced GABAergic transmission can be rescued by increasing dopamine tone and vice versa. We also examined the role of this dopamine–GABA interaction for the termination of working memory and found that the extent of GABAergic excitation needed to reset the PFC network begins to occur when the activity of fast-spiking interneurons surpasses 40 Hz. Together, these results indicate that the capability of the PFC to sustain working memory and network stability depends on a robust interplay of compensatory mechanisms between dopamine tone and the activity of local GABAergic interneurons. 相似文献
164.
165.
Mohsen Mohandes Sergio Rojas Jordi Guarinos Judit Rodríguez Francisco Fernández Cristina Moreno Alfredo Bardají 《Journal of the Saudi Heart Association》2017,29(1):60-65
We describe a case of septal branch perforation during percutaneous coronary intervention of a right coronary artery chronic total occlusion. The septal branch perforation was treated with administration of autologous fat into the septal branch with significant reduction of extravasation. This treatment was followed by prolonged balloon inflation at the exit point of the septal branch in the donor artery which definitively sealed the perforation. 相似文献
166.
Gabriel de Deus Vieira Sergio de Almeida Basano Tony Hiroshi Katsuragawa Luís Marcelo Aranha Camargo 《Revista do Instituto de Medicina Tropical de S?o Paulo》2014,56(6):493-497
Mosquito nets treated with long-lasting insecticide (LLINs),
when used in compliance with guidelines of the World Health Organization, may be
effective for malaria vector control. In 2012, approximately 150,000 LLINs were
installed in nine municipalities in the state of Rondônia. However, no
studies have assessed their impact on the reduction of malaria incidence. This
study analyzed secondary data of malaria incidence, in order to assess the
impact of LLINs on the annual parasite incidence (API). The results
showed no statistically significant differences in API one year after LLIN
installation when compared to municipalities without LLINs. The adoption of
measures for malaria vector control should be associated with epidemiological
studies and evaluations of their use and efficiency, with the aim of offering
convincing advantages that justify their implementation and limit malaria
infection in the Amazon Region. 相似文献
167.
Hua Qin Brendan T Keenan Diego R Mazzotti Fernando Vaquerizo-Villar Jan F Kraemer Niels Wessel Sergio Tufik Lia Bittencourt Peter A Cistulli Philip de Chazal Kate Sutherland Bhajan Singh Allan I Pack Ning-Hung Chen Ingo Fietze Thorarinn Gislason Steven Holfinger Ulysses J Magalang Thomas Penzel 《Sleep》2021,44(5)
Study ObjectivesPatients with obstructive sleep apnea (OSA) exhibit heterogeneous heart rate variability (HRV) during wakefulness and sleep. We investigated the influence of OSA severity on HRV parameters during wakefulness in a large international clinical sample.Methods1247 subjects (426 without OSA and 821 patients with OSA) were enrolled from the Sleep Apnea Global Interdisciplinary Consortium. HRV parameters were calculated during a 5-minute wakefulness period with spontaneous breathing prior to the sleep study, using time-domain, frequency-domain and nonlinear methods. Differences in HRV were evaluated among groups using analysis of covariance, controlling for relevant covariates.ResultsPatients with OSA showed significantly lower time-domain variations and less complexity of heartbeats compared to individuals without OSA. Those with severe OSA had remarkably reduced HRV compared to all other groups. Compared to non-OSA patients, those with severe OSA had lower HRV based on SDNN (adjusted mean: 37.4 vs. 46.2 ms; p < 0.0001), RMSSD (21.5 vs. 27.9 ms; p < 0.0001), ShanEn (1.83 vs. 2.01; p < 0.0001), and Forbword (36.7 vs. 33.0; p = 0.0001). While no differences were found in frequency-domain measures overall, among obese patients there was a shift to sympathetic dominance in severe OSA, with a higher LF/HF ratio compared to obese non-OSA patients (4.2 vs. 2.7; p = 0.009).ConclusionsTime-domain and nonlinear HRV measures during wakefulness are associated with OSA severity, with severe patients having remarkably reduced and less complex HRV. Frequency-domain measures show a shift to sympathetic dominance only in obese OSA patients. Thus, HRV during wakefulness could provide additional information about cardiovascular physiology in OSA patients.Clinical Trial Information: A Prospective Observational Cohort to Study the Genetics of Obstructive Sleep Apnea and Associated Co-Morbidities (German Clinical Trials Register - DKRS, DRKS00003966) https://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00003966 相似文献
168.
Sara Ariotti Maarten van Leeuwen Salvatore Brugaletta Sergio Leonardi K. Martijn Akkerhuis Stefano F. Rimoldi Gladys N. Janssens Luis Ortega-Paz Umberto Gianni Jan C. van den Berge Alexios Karagiannis Stephan Windecker Marco Valgimigli 《Journal of the American College of Cardiology》2018,71(11):1289-1291
169.
Dáfne Cardoso Bourguignon da Silva Gláucia Toribio Finoti Seixas Orlei Ribeiro de Araujo Rodrigo Genaro Arduini Fabianne Altruda de Moraes Costa Carlesse Antonio Sergio Petrilli 《The Brazilian journal of infectious diseases》2012,16(4):361-365
BackgroundUsual treatment regimens with vancomycin often fail to provide adequate serum levels in patients with severe infections.MethodsRetrospective analysis of vancomycin trough serum measurements. The following parameters were calculated by Bayesian analysis: vancomycin clearance, distribution volume, and peak estimated concentrations. The area under the concentration curve (AUC) (total daily dose/24 h clearance of vancomycin) was used to determine the effectiveness of treatment through the ratio of AUC/minimum inhibitory concentration (MIC) above 400, using MIC = 1 μg/mL, based on isolates of Staphylococci in cultures.ResultsSixty-one vancomycin trough measurements were analyzed in 31 patients. AUC/MIC > 400 was obtained in 34 out of 61 dosages (55.7%), but the mean vancomycin dose required to achieve these levels was 81 mg/kg/day. In cases where the usual doses were administered (40–60 mg/kg/day), AUC/MIC > 400 was obtained in nine out of 18 dosages (50%), in 13 patients. Trough serum concentrations above 15 mg/L presented a positive predictive value of 100% and a negative predictive value of 71% for AUC/MIC > 400.ConclusionHigher than usual vancomycin doses may be required to treat staphylococcal infections in children with oncologic/hematologic diseases. Since the best known predictor of efficacy is the AUC/MIC ratio, serum trough concentrations must be analyzed in conjunction with MICs of prevalent Staphylococci and pharmacokinetic tools such as Bayesian analysis. 相似文献
170.
Francesco Paolo Caronia Alfonso Fiorelli Mario Santini Sergio Castorina 《General thoracic and cardiovascular surgery》2016,64(10):625-628
Persistent tracheal fistula after tracheostomy decannulation is a recognized sequel to long-term tracheostomy use, causing important morbidity including difficult to vocalization and control of air secretions, recurrent pulmonary infections, and cosmetic and social problems. Herein, we reported a new method for closure of persistent tracheocutaneous fistula with rib cartilages. Compared to other techniques previously reported, the variations of our strategy were the use of temporary metal-covered tracheal stent and the hinged turnover skin bi-flaps reinforced with rib cartilage grafts. Rib cartilages were useful in order to reconstruct the trachea and prevent stenosis. Since it become difficult to obtain the maintenance of the trachea stability until healing of suture was well established, a covered metallic stent was also inserted to avoid flap collapse. The stent was removed 3 months later. Six months follow-up showed normal tracheal patency. 相似文献