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David W. Hilbert William L. Smith Sean G. Chadwick Geoffrey Toner Eli Mordechai Martin E. Adelson Tina J. Aguin Jack D. Sobel Scott E. Gygax 《Journal of clinical microbiology》2016,54(4):1017-1024
Bacterial vaginosis (BV) is the most common gynecological infection in the United States. Diagnosis based on Amsel''s criteria can be challenging and can be aided by laboratory-based testing. A standard method for diagnosis in research studies is enumeration of bacterial morphotypes of a Gram-stained vaginal smear (i.e., Nugent scoring). However, this technique is subjective, requires specialized training, and is not widely available. Therefore, a highly accurate molecular assay for the diagnosis of BV would be of great utility. We analyzed 385 vaginal specimens collected prospectively from subjects who were evaluated for BV by clinical signs and Nugent scoring. We analyzed quantitative real-time PCR (qPCR) assays on DNA extracted from these specimens to quantify nine organisms associated with vaginal health or disease: Gardnerella vaginalis, Atopobium vaginae, BV-associated bacteria 2 (BVAB2, an uncultured member of the order Clostridiales), Megasphaera phylotype 1 or 2, Lactobacillus iners, Lactobacillus crispatus, Lactobacillus gasseri, and Lactobacillus jensenii. We generated a logistic regression model that identified G. vaginalis, A. vaginae, and Megasphaera phylotypes 1 and 2 as the organisms for which quantification provided the most accurate diagnosis of symptomatic BV, as defined by Amsel''s criteria and Nugent scoring, with 92% sensitivity, 95% specificity, 94% positive predictive value, and 94% negative predictive value. The inclusion of Lactobacillus spp. did not contribute sufficiently to the quantitative model for symptomatic BV detection. This molecular assay is a highly accurate laboratory tool to assist in the diagnosis of symptomatic BV. 相似文献
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Alejandro Gonzalez Sean McLachlan Francis Keaney 《International journal of psychiatry in clinical practice》2013,17(3):159-167
The misuse of anabolic androgenic steroids (AAS) and other performance enhancing (ergogenic) drugs remains largely unrecognized by many health professionals. The real extent of the problem is unknown, probably as a result of a combination of various methodological difficulties. Examples include poor definition of cases, obstacle in recruiting large enough samples for longitudinal follow-up, ethical issues as AAS are obtained from the black market and the covert nature of the problem itself. Our review attempts to alert psychiatrists and mental health professionals to the risks associated with these compounds. We cover the pharmacology, epidemiology, the use and misuse and relevant complications. 相似文献
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