全文获取类型
收费全文 | 625篇 |
免费 | 30篇 |
国内免费 | 5篇 |
专业分类
耳鼻咽喉 | 3篇 |
儿科学 | 48篇 |
妇产科学 | 7篇 |
基础医学 | 63篇 |
口腔科学 | 16篇 |
临床医学 | 95篇 |
内科学 | 136篇 |
皮肤病学 | 32篇 |
神经病学 | 16篇 |
特种医学 | 124篇 |
外科学 | 17篇 |
综合类 | 27篇 |
预防医学 | 23篇 |
眼科学 | 4篇 |
药学 | 20篇 |
1篇 | |
肿瘤学 | 28篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 2篇 |
2020年 | 4篇 |
2019年 | 6篇 |
2018年 | 11篇 |
2017年 | 4篇 |
2016年 | 9篇 |
2015年 | 10篇 |
2014年 | 13篇 |
2013年 | 16篇 |
2012年 | 8篇 |
2011年 | 17篇 |
2010年 | 29篇 |
2009年 | 28篇 |
2008年 | 12篇 |
2007年 | 22篇 |
2006年 | 21篇 |
2005年 | 18篇 |
2004年 | 11篇 |
2003年 | 9篇 |
2002年 | 13篇 |
2001年 | 11篇 |
2000年 | 8篇 |
1999年 | 9篇 |
1998年 | 37篇 |
1997年 | 46篇 |
1996年 | 39篇 |
1995年 | 20篇 |
1994年 | 29篇 |
1993年 | 23篇 |
1992年 | 12篇 |
1991年 | 10篇 |
1990年 | 12篇 |
1989年 | 23篇 |
1988年 | 21篇 |
1987年 | 10篇 |
1986年 | 8篇 |
1985年 | 5篇 |
1984年 | 14篇 |
1983年 | 14篇 |
1982年 | 12篇 |
1981年 | 11篇 |
1980年 | 7篇 |
1979年 | 1篇 |
1978年 | 4篇 |
1977年 | 2篇 |
1976年 | 4篇 |
1969年 | 1篇 |
排序方式: 共有660条查询结果,搜索用时 0 毫秒
81.
82.
B Ruibal-Ares L Belmonte P Baré M Scolnik M F Palacios C Bayo-Hanza C M Galmarini G Mendez M M de Bracco 《Journal of human virology》2001,4(4):200-213
OBJECTIVES: To study the factors that determine malignant B cell growth in human immunodeficiency virus type 1 (HIV-1)-infected patients. STUDY DESIGN: B-cell lines (lymphocyte cell lines [LCL]) were developed after nonstimulated culture of peripheral blood mononuclear cells (PBMC) from HIV-1-positive (HIV-1(+)) patients. Human immunodeficiency virus type 1 replication in culture, Epstein-Barr virus (EBV) latent oncogene expression, and cell-to-cell interaction were studied after nonstimulated culture of HIV-1(+) PBMC, analyzing their contribution to LCL appearance. METHODS: Nonstimulated PBMC cultures of HIV-1(+) PBMC and controls (N-PBMC) were established. Lymphocyte cell lines were characterized. Epstein-Barr virus latent membrane protein 1 (LMP-1) and Epstein-Barr nuclear antigen 2 were detected by polymerase chain reaction (PCR). Clonality of LCL was determined by light chain restriction (flow cytometry) and immunoglobulin H chain rearrangement (semi-nested PCR). Peripheral blood mononuclear cell phenotypes were studied at different intervals of culture. RESULTS: Lymphocyte cell lines were obtained in 73% of HIV-1(+) PBMC cultures, compared with 6% in N-PBMC. All LCL were EBV-positive (EBV(+)). B-cell lineage was established, and up to 12 different B-cell clones were expanded from the same individual. Occurrence of LCL was more frequent in cultures with HIV-1 replication, high LMP-1 expression in viable B cells, and high CD4:CD8 ratio. Human immunodeficiency virus type 1 replication persisted in 53% of the LCL. CONCLUSIONS: In vitro HIV-1 replication and persistence of viable EBV(+) lymphoblasts favor spontaneous in vitro outgrowth of LCL in HIV-1(+) patients. 相似文献
83.
84.
Waldmann TA; Goldman CK; Bongiovanni KF; Sharrow SO; Davey MP; Cease KB; Greenberg SJ; Longo DL 《Blood》1988,72(5):1805-1816
Human T-cell lymphotropic virus I (HTLV-I)-induced adult T-cell leukemia (ATL) cells constitutively express interleukin-2 (IL-2) receptors identified by the anti-Tac monoclonal antibody (MoAb), whereas normal resting cells do not. This observation provided the scientific basis for a trial of intravenous anti-Tac in the treatment of nine patients with ATL. The patients did not suffer untoward reactions and did not have a reduction in the normal formed elements of the blood, and only one of the nine produced antibodies to the anti-Tac MoAb. Three patients had transient mixed, partial, or complete remissions lasting from 1 to more than 8 months after anti-Tac therapy, as assessed by routine hematologic tests, immunofluorescence analysis of circulating cells, and molecular genetic analysis of HTLV-I provirus integration and of the T-cell receptor gene rearrangement. The precise mechanism of the antitumor effects is unclear; however, the use of a MoAb that prevents the interaction of IL-2 with its receptor on ATL cells provides a rational approach for the treatment of this malignancy. 相似文献
85.
86.
87.
88.
Murakami T; Baron RL; Peterson MS; Oliver JH rd; Davis PL; Confer SR; Federle MP 《Radiology》1996,200(1):69
89.
J-B Liu† M Li‡§ H Chen† S-Q Zhong† S Yang¶ W-D Du¶ J-H Hao†† T-S Zhang‡‡ X-J Zhang¶ MP Zeegers§§¶¶ 《Journal of the European Academy of Dermatology and Venereology》2007,21(2):205-213
BACKGROUND: Linkage and association studies suggest that the human leucocyte antigen (HLA) region may be involved in the genetic susceptibility of vitiligo. HLA-A2 has been reported to be associated with vitiligo in some, but not all, studies. OBJECTIVE: To identify sources of the heterogeneity among studies and to quantify effect estimates, we examined the association of HLA-A2 with vitiligo in a meta-analysis of all observational studies comparing the frequencies of HLA-A2 between vitiligo individuals and controls during 1966-2005. METHODS: The summary odds ratio (OR) was calculated by using a fixed- or a random-effects model. Meta-regression analysis was undertaken to investigate the effects of study characteristics on the pooled OR. RESULTS: Eleven case-controlled studies fulfilled our inclusion criteria. The studies identified a total of 777 patients and 4820 controls. Meta-analysis showed a significantly increased frequency of HLA-A2 in vitiligo among cases [OR = 2.07, 95% confidence interval (CI) 1.67-2.58]. Heterogeneity was explained by the quality of the study and the ethnic background of the participants. Meta-regression analysis further showed that the percentage of familial vitiligo among the subjects had a significant effect on the pooled OR (P = 0.008). No study had a significant effect on the pooled OR and no publication bias presented in the studies analysed (P = 0.688). CONCLUSION: These findings strongly suggest an association between HLA-A2 and vitiligo. 相似文献
90.
X-linked hypophosphatemia in adults: prevalence of skeletal radiographic and scintigraphic features 总被引:3,自引:0,他引:3
The radiologic studies of 38 essentially untreated adults with X-linked hypophosphatemia (XLH) were reviewed to determine the prevalence of radiologic features, to compare the findings in men and in women, and to elucidate the natural history of the disease by comparing the findings in young, intermediate-age, and older patients. Bone-reinforcement lines were common, but no characteristic mineral mass alteration was established. Looser zones were more prevalent in older subjects. Osteoarthritis was common, occurring in the ankles, knees, feet, sacroiliac joints, and wrists. Enthesopathy was infrequent in the younger group but was present in every member of the intermediate and older groups and was often accompanied by extra ossicles. Curvatures of the lower-extremity long bones were common in all age groups. Three new skeletal alterations in XLH were found to be common: flaring of the iliac wings, trapezoidal distal femoral condyles, and alterations in talar morphology, including shortening of the talar neck and flattening of the talar dome. Technetium-99m methylene diphosphonate scintigrams of 17 subjects were often abnormal, depicting bowing deformity and focal tracer accumulation in diaphyseal cortices and in periarticular and extraarticular regions. The mean metabolic index was moderately elevated (4.0). Both radiographic and scintigraphic findings were more severe in men, consistent with hemizygosity. The natural history of untreated XLH in both sexes is characterized by the development of a variety of age-related skeletal abnormalities during adulthood. 相似文献