全文获取类型
收费全文 | 757篇 |
免费 | 48篇 |
国内免费 | 27篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 42篇 |
妇产科学 | 9篇 |
基础医学 | 63篇 |
口腔科学 | 29篇 |
临床医学 | 71篇 |
内科学 | 167篇 |
皮肤病学 | 21篇 |
神经病学 | 23篇 |
特种医学 | 115篇 |
外科学 | 107篇 |
综合类 | 11篇 |
预防医学 | 44篇 |
眼科学 | 2篇 |
药学 | 44篇 |
中国医学 | 2篇 |
肿瘤学 | 81篇 |
出版年
2023年 | 18篇 |
2022年 | 7篇 |
2021年 | 4篇 |
2020年 | 7篇 |
2019年 | 19篇 |
2018年 | 17篇 |
2017年 | 13篇 |
2016年 | 13篇 |
2015年 | 17篇 |
2014年 | 12篇 |
2013年 | 43篇 |
2012年 | 13篇 |
2011年 | 27篇 |
2010年 | 29篇 |
2009年 | 32篇 |
2008年 | 24篇 |
2007年 | 24篇 |
2006年 | 17篇 |
2005年 | 22篇 |
2004年 | 26篇 |
2003年 | 11篇 |
2002年 | 13篇 |
2001年 | 12篇 |
2000年 | 11篇 |
1999年 | 15篇 |
1998年 | 30篇 |
1997年 | 32篇 |
1996年 | 29篇 |
1995年 | 30篇 |
1994年 | 28篇 |
1993年 | 20篇 |
1992年 | 14篇 |
1991年 | 7篇 |
1990年 | 14篇 |
1989年 | 20篇 |
1988年 | 20篇 |
1987年 | 15篇 |
1986年 | 21篇 |
1985年 | 14篇 |
1984年 | 6篇 |
1983年 | 9篇 |
1982年 | 17篇 |
1981年 | 15篇 |
1980年 | 8篇 |
1979年 | 7篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1975年 | 9篇 |
1969年 | 2篇 |
排序方式: 共有832条查询结果,搜索用时 218 毫秒
51.
Clinical pharmacology of GW280430A in humans 总被引:11,自引:0,他引:11
Belmont MR Lien CA Tjan J Bradley E Stein B Patel SS Savarese JJ 《Anesthesiology》2004,100(4):768-773
BACKGROUND: An ultrashort-acting nondepolarizing neuromuscular blocking agent that could be an alternative to succinylcholine has been the focus of a concerted effort in the field of muscle relaxants. GW280430A showed a promising pharmacodynamic profile in preclinical work and a wide margin of safety and so was selected for study in humans. METHODS: Thirty-one volunteers participated in this study, which determined the dose producing 95% block (ED95) and the safety and pharmacodynamics of increasing ED95 multiples. Anesthesia was induced and maintained with propofol, midazolam, and fentanyl. Neuromuscular transmission was measured at the adductor pollicis using ulnar nerve stimulation, and responses were recorded continuously by standard mechanomyographic monitoring. RESULTS: The ED95 for GW280430A is 0.19 mg/kg. The time to onset of 90% block ranged from 1.3 to 2.1 min, depending on the dose. Clinical durations ranged from 4.7 to 10.1 min and increased with increasing dose. Five to 95% and 25-75% recovery rates were approximately 7 and 3 min, respectively, and were independent of the dose administered. Transient cardiovascular side effects were observed at doses beginning at 3 x ED95 and above and were suggestive of histamine release. Most volunteers receiving 4 x ED95 exhibited plasma histamine concentrations indicative of significant histamine release. CONCLUSIONS: GW280430A has a rapid onset and ultrashort duration of action. The recovery rate is rapid, predictable, and independent of dose. Doses at least up to 2.5 x ED95 seem to be free of side effects and seem to be able to provide relaxation within 60-90 s. 相似文献
52.
O'Brien MF Connolly SS Kelly DG O'Brien A Quinlan DM Mulvin DW 《Irish journal of medical science》2004,173(1):23-26
Background Patients with prostate cancer with a pre-operative prostate-specific antigen (PSA) τ;15ng/ml who undergo radical retropubic
prostatectomy (RRP) generally do not have a good outcome, yet may have organ-confined cancer and should be offered the option
of surgery.
Aim To assess the outcome of patients who underwent RRP with a pre-operative PSA ≥ 15ng/ml.
Methods Thirty-four patients, mean pre-operative PSA: 25.46ng/ml (15.03–76.6) and mean Gleason score: 6.4 (5–9) were assessed.
Results Two groups were identified. Group I: 41% (14/34) have no biochemical recurrence to mean follow up of 58 months (30–106).
Mean PSA: 18.8ng/ml (15.03–25.84). Mean Gleason score: 6.1 (5–7). Clinical stage: T1c in 80%. No patient had seminal vesicle
or lymph node involvement. Group II: 59% (20/34) have biochemical recurrence or died (3) from their disease to mean follow
up of 66 months (36–98). Mean PSA: 28.9ng/ml (15.28–76.6). Mean Gleason score: 6.7 (5–9). Clinical stage: T1c in 25%. Eleven
patients had seminal vesicle (8) involvement or positive lymph nodes (3) or both (2).
Conclusion RRP seems feasible in patients whose pre-operative PSA is between 15 and 25ng/ml with stage T1c, Gleason score ≤ 7 and negative
lymph node frozen section. 相似文献
53.
Prevention of chemotherapy and radiation toxicity with glutamine 总被引:19,自引:0,他引:19
GOALS OF THE WORK: Malignancy produces a state of physiologic stress that is characterized by a relative deficiency of glutamine, a condition that is further exacerbated by the effects of cancer treatment. Glutamine deficiency may impact on normal tissue tolerance to antitumor treatment, and may lead to dose reductions and compromised treatment outcome. Providing supplemental glutamine during cancer treatment has the potential to abrogate treatment-related toxicity. We reviewed the available data on the use of glutamine to decrease the incidence and severity of adverse effects due to chemotherapy and/or radiation in cancer patients. METHODS: We performed a search of the MEDLINE database during the time period 1980-2003, and reviewed the English language literature of both human and animal studies pertaining to the use of glutamine in subjects with cancer. We also manually searched the bibliographies of published articles for relevant references. MAIN RESULTS: The available evidence suggests that glutamine supplementation may decrease the incidence and/or severity of chemotherapy-associated mucositis, irinotecan-associated diarrhea, paclitaxel-induced neuropathy, hepatic veno-occlusive disease in the setting of high dose chemotherapy and stem cell transplantation, and the cardiotoxicity that accompanies anthracycline use. Oral glutamine supplementation may enhance the therapeutic index by protecting normal tissues from, and sensitizing tumor cells to chemotherapy and radiation-related injury. CONCLUSIONS: The role of glutamine in the prevention of chemotherapy and radiation-induced toxicity is evolving. Glutamine supplementation is inexpensive and it may reduce the incidence of gastrointestinal, neurologic, and possibly cardiac complications of cancer therapy. Further studies, particularly placebo-controlled phase III trials, are needed to define its role in chemotherapy-induced toxicity. 相似文献
54.
Interleukin-11 receptor expression in primary ovarian carcinomas 总被引:2,自引:0,他引:2
Campbell CL Guardiani R Ollari C Nelson BE Quesenberry PJ Savarese TM 《Gynecologic oncology》2001,80(2):121-127
55.
Duodenal somatostatinomas associated with von Recklinghausen's neurofibromatosis. Apropos of 2 cases
Cohen C Heymann MF Michenet P Memeteau F Saint-Marc O Emy P Maitre F Le Bodic MF 《Annales de pathologie》2000,20(6):609-611
Somatostatinomas are rare neuroendocrine tumors; they are essentially located in the pancreas and in the duodenum. The association with a neurofibromatosis type I is especially observed when the tumor is located in the ampulla of Vater. These tumors are not associated with a "somatostatin syndrome", but often present with gastrointestinal bleeding, abdominal pain and obstructive jaundice. The diagnosis is confirmed by immunohistochemical studies. The aim of this study is to report 2 cases of metastazing duodenal periampullary somatostatinomas associated with von Recklinghausen's disease and to discuss the prognosis of these tumors. Future genetic research are necessary as point out the familial feature of this association in one of our cases. 相似文献
56.
A. B. Kornblith J. E. Herndon II E. Zuckerman P. A. Godley D. Savarese N. J. Vogelzang J. C. Holland 《Annals of oncology》2001,12(5):633-641
Objectives:The quality of life (QoL) of 44 men with HRPC and 37 partners (primary caregivers, most residing with the patient) was assessed in a multicenter Phase II trial of docetaxel, estramustine and low dose hydrocortisone (CALGB 9780). A secondary objective was to test the feasibility of assessing partners QoL in a cooperative group setting.
Patients and methods:Patients and partners were separately interviewed by telephone at baseline, two, four and six months by a single trained research interviewer. Patients QoL was measured by the FACT-P, Mental Health Inventory-17 (MHI-17), Brief Pain Inventory (BPI), a two-day log of pain medications, and the OARS for co-morbid conditions. Partners QoL was measured by the MHI-17, Caregiver Burden Interview, and co-morbid conditions.
Results:The QoL study refusal rates were low for patients (4%) and partners (3%). Although patients tended to experience greater treatment side effects in the first two months (FACT Physical Well-Being item, P = 0.057), their cancer-specific emotions (e.g., worrying about worsening health) significantly improved at two and four months (FACT-Emotional Well-Being, P = 0.003, P = 0.03, respectively), as did their prostate cancer-specific physical problems (e.g., urination, pain), at two and four months (FACT-P, P = 0.001, P = 0.005, respectively). Partners anxiety significantly decreased over time (MHI,P < 0.05). Patients quality of life at two months was significantly related to their clinical response (FACT-P total and prostate cancer-specific problems, P < 0.05), and their clinical response was significantly related to a decrease in their partners anxiety at two months (MHI, P < 0.05).
Conclusions:Despite feeling worse from side effects, patients prostate cancer-specific problems and emotional state significantly improved in the first four months of treatment. With treatment significantly affecting both patients and partners lives, and the successful assessment of partners QoL, QoL of both patients and partners could be used as important endpoints in selected clinical trials. 相似文献
57.
高效液相色谱法测定寒痹停片中士的宁含量 总被引:6,自引:0,他引:6
目的:建立用HPLC测定寒痹停片中士的含量的方法。方法:氰基柱;流动相-甲醇-水-三乙胺-乙酸(9800:155:15:30);紫外检测波长254nm。结果:在4~20ug/ml范围内,标准曲线回归方程为:Y=-2803+8967x(r=0.9997),RSD=1.65%?加样回收率的平均值为99.82%。结论:实验表明,这是一个适用于生产控制和产品质量检验的简单、快速、准确的方法。 相似文献
58.
Campbell CL Savarese DM Quesenberry PJ Savarese TM 《International journal of cancer. Journal international du cancer》1999,80(6):868-874
The cytokines that regulate angiogenesis in normal and malignant prostate tissue are not well studied. Using an RT-PCR-based screen, we observed that cultured, low-passage normal human prostate epithelial cells (PrECs) express a variety of cytokines which have been shown to have angiogenic and/or endothelial cell-activating properties in various systems. These include vascular endothelial growth factor (VEGF), basic fibroblastic growth factor (bFGF), transforming growth factor-alpha (TGF-alpha), transforming growth factor-beta (TGF-beta), interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF). Expression of VEGF, bFGF, GM-CSF, G-CSF, TGF-alpha and TNF-alpha in these cells was confirmed by immunohistochemistry. Culture medium conditioned by normal human PrECs for periods of up to 96 hr were found to contain VEGF, GM-CSF, G-CSF, IL-8, TGF-beta1 and TGF-beta2 but not TNF-alpha or bFGF, as determined by ELISA. Of these, VEGF was by far the most prominently expressed angiogenic cytokine (approx. 2,500 pg/ml conditioned medium at 96 hr vs. 30 to 100 pg/ml conditioned medium for the other cytokines). PrEC-conditioned medium induced an approximately 2-fold stimulation of [3H]-thymidine incorporation in cultured human umbilical cord endothelial cells (HUVECs) deprived of the endothelial growth factors VEGF and bFGF; this stimulation was abolished by neutralizing antibodies directed against VEGF but not bFGF, IL-8, GM-CSF or TNF-alpha. VEGF expression by PrECs was not markedly altered by administration or deprivation of other angiogenic cytokines for which these cells have receptors, suggesting that there is not a hierarchy of cytokines controlling its expression; however, retinoic acid, a component of PrEC growth medium, was found to modestly suppress VEGF at physiological concentrations (0.1 ng/ml). These data suggest that normal PrECs express a variety of angiogenic cytokines, most prominently VEGF, to recruit a supporting vasculature, even in culture. Our data also suggest that the ability of malignant PrECs to stimulate angiogenesis may be intrinsic and does not need to be acquired during oncogenesis. 相似文献
59.
MF Whitfield 《Archives of disease in childhood》1980,55(2):139-142
A third case of chondrodysplasia punctata after exposure to warfarin alone in early pregnancy is described. The clinical course of the child during the first 18 months is outlined. The use of warfarin in early pregnancy must be avoided because of its established teratogenic effects in causing this syndrome, in addition to an overall increase in perinatal mortality. 相似文献
60.
Posteroanterior (PA) and caudally angulated PA views were obtained in 20 patients undergoing routine coronary arteriography. Although the left main coronary artery (LMCA) was seen well on both views in all patients, the PA-caudal view improved depiction of the LMCA bifurcation in 15 (75%). In addition, the PA-caudal view markedly improved depiction of the circumflex artery, affording optimal depiction of this artery and its branches in 78%-89% of patients. Neither the PA nor the PA-caudal view allowed adequate depiction of the left anterior descending artery. Thus, the PA-caudal view should supplant the PA view in routine coronary arteriography. 相似文献