全文获取类型
收费全文 | 1089篇 |
免费 | 60篇 |
国内免费 | 16篇 |
专业分类
耳鼻咽喉 | 7篇 |
儿科学 | 26篇 |
妇产科学 | 11篇 |
基础医学 | 185篇 |
口腔科学 | 6篇 |
临床医学 | 87篇 |
内科学 | 229篇 |
皮肤病学 | 29篇 |
神经病学 | 91篇 |
特种医学 | 15篇 |
外科学 | 122篇 |
综合类 | 24篇 |
一般理论 | 1篇 |
预防医学 | 167篇 |
眼科学 | 8篇 |
药学 | 83篇 |
中国医学 | 3篇 |
肿瘤学 | 71篇 |
出版年
2024年 | 3篇 |
2023年 | 17篇 |
2022年 | 36篇 |
2021年 | 39篇 |
2020年 | 21篇 |
2019年 | 44篇 |
2018年 | 37篇 |
2017年 | 24篇 |
2016年 | 35篇 |
2015年 | 43篇 |
2014年 | 44篇 |
2013年 | 59篇 |
2012年 | 82篇 |
2011年 | 101篇 |
2010年 | 53篇 |
2009年 | 47篇 |
2008年 | 57篇 |
2007年 | 64篇 |
2006年 | 65篇 |
2005年 | 68篇 |
2004年 | 51篇 |
2003年 | 56篇 |
2002年 | 48篇 |
2001年 | 8篇 |
2000年 | 4篇 |
1999年 | 1篇 |
1998年 | 5篇 |
1997年 | 5篇 |
1996年 | 8篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 5篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1986年 | 4篇 |
1985年 | 5篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有1165条查询结果,搜索用时 15 毫秒
31.
Performance of the BioPlex 2200 Flow Immunoassay in Critical Cases of Serodiagnosis of Toxoplasmosis
Nicolas Guigue Jean Menotti Samia Hamane Francis Derouin Yves Jean-Fran?ois Garin 《Clinical and Vaccine Immunology : CVI》2014,21(4):496-500
The BioPlex 2200 automated analyzer (Bio-Rad Laboratories, Hercules, CA) is a recently developed multiplex analyzer that enables the detection of anti-Toxoplasma, -rubella, and -cytomegalovirus antibodies in the same assay. The aim of this study was to compare this new technology (using the BioPlex 2200 ToRC IgG/IgM kit) in critical cases of serodiagnosis of toxoplasmosis (acute, chronic, or congenital infections and cases with discrepant results) to the technologies used in our routine practice, i.e., the Platelia IgG/IgM enzyme-linked immunosorbent assays (ELISAs) (Bio-Rad Laboratories) and the Toxo-Screen direct agglutination assay (bioMérieux, Lyon, France). Overall, most cases of false-positive/negative results obtained with the Platelia IgG or Toxo-Screen assay were corrected by the BioPlex 2200 ToRC IgG (87.5%). Furthermore, the analysis of 35 sequences of sera showed a trend toward a more rapid decrease of IgM titers by BioPlex 2200 than by Platelia. These results for IgM detection can be explained by a weaker detection of residual IgM. Indeed, among 23 serum samples from patients with probable past infection with long-lasting IgM (Platelia M positive and IgG avidity index, ≥0.5), the BioPlex 2200 Toxoplasma IgM assay was positive for only 11 serum samples. In our panel of critical cases comprising 156 serum and 6 cord blood samples from 103 patients with acute, chronic, or congenital infection, the BioPlex 2200 IgG assay was a sensitive (97.8%) and specific (91.3%) method for IgG detection. The high specificity (97.4%) of IgM detection combined with the shorter kinetics of IgM titers may considerably reduce the number of residual IgM detections, thus yielding more precise diagnoses of acute infections. 相似文献
32.
Sarah Dellire Brune Joannard Mazouz Benderdouche Anselme Mingui Maud Gits-Muselli Samia Hamane Alexandre Alanio Antoine Petit Germaine Gabison Martine Bagot Stphane Bretagne 《Emerging infectious diseases》2022,28(1):224
We describe 7 cases of extensive tinea corporis since 2018 in a hospital in Paris, France, after failure to cure with terbinafine. Molecular analysis indicated Trichophyton mentagrophytes internal transcribed spacer type VIII (T. indotineae). This strain, which has mutations in the squalene epoxidase gene, is spreading on the Indian subcontinent. 相似文献
33.
Psoriasis management is complex and challenging. It should be tailored for each patient. Treatment strategy differs according to patient's age, sex, disease type, disease severity, burden on patient's quality of life, comorbidities, involvement of specific sites, and pregnancy. The choice of the appropriate therapeutic must take into consideration the availability, the price, and the patient's preferences. It is very important that the chosen treatment is not more unpleasant, intolerable, or dangerous than the disease itself. According to the disease type, severity, and effect on patient's quality of life, dermatologist chooses whether to start with topical therapy, phototherapy or systemic therapy, or a combination of two or more of them. Under each category, there are different types of therapies that can be the first line therapeutics, second line, or even contraindicated. In this compendium, we provide dermatologists with different treatment plans considering all the mentioned variables so that a dermatologist can choose the optimum plan for the patient. 相似文献
34.
35.
The Mediterranean area represents the area of land that borders the Mediterranean basin. It is composed of several countries that share many geographic and racial characteristics. Although Mediterraneans seem to share common skin type and are subjected to similar enviromental factors, they still represent a genetic and socioeconomic diversity. True prevalence of pigmentary disorders in this area depends on large epidemiologic studies, including countries that are not available. This article, however, highlights and classifies the most important developmental (heritable-genetic) and acquired pigmentary disorders seen and reported in this important area of the world. 相似文献
36.
The modulation of co-stimulatory pathways represents a novel therapeutic strategy to regulate autoimmune diseases. Auto-reactive
CD4+ T cells play a critical role in initiating the immune response leading to inflammation and autoimmune diseases. Blocking
co-stimulatory signals prevents T-cell activation, thus diminishing autoimmune responses and possibly preventing the progression
of autoimmune disease. Blockade of several co-stimulatory pathways has been investigated in animal models and has led to clinical
trials testing specific blocking agents in humans. In this review we will describe the role of co-stimulatory pathways, primarily
the CD28-B7 pathway, in autoimmune diseases, and we will present in vivo and in vitro studies supporting the efficacy of co-stimulation blockade in animal models of autoimmune disease. Finally, we will discuss
the clinical therapeutic efficacy of blocking monoclonal antibodies in preventing or reducing auto-antigen driven T-cell activation
in humans with particular attention to the CD28/B7 pathway. Inhibiting co-stimulatory molecule interactions by using monoclonal
antibodies seems to be an original approach to regulate autoimmune diseases in humans. 相似文献
37.
Background
Social anxiety disorder (SAD) has been repeatedly shown to be very prevalent in the Western society with prevalence rates of 10% or above. However, very few studies have been performed in the Middle East and in Arab countries.Methods
A total of 300 Israeli students participated in our study and were administered the Liebowitz Social Anxiety Scale (LSAS), the Cheek and Buss Shyness Questionnaire (CBSQ), and a sociodemographic questionnaire.Results
A total of 153 Jewish and 147 Arab students participated in the survey. Social anxiety disorder was found in 12.33% of the sample, according to the LSAS cutoff score of more than 60. The 2 subsamples had similar LSAS and CBSQ scores and similar SAD-positive rates (LSAS >60). Females had higher scores on the LSAS, as were those without a spouse and those who had been in psychological treatment. Based on a regression analysis, the significant predictors of the LSAS score were the CBSQ score and female sex. A very high correlation was found between the LSAS and the CBSQ scores.Conclusions
Although our sample is not representative of the whole Israeli population, we conclude that SAD and shyness were similarly prevalent in Jewish and Arab students in Israel. Social anxiety disorder scores were higher among females, those without a spouse, and those who received psychological treatment. Further studies on the clinical and cultural characteristics of SAD in Israeli subcultures would add to the growing body of knowledge on SAD in various cultures. 相似文献38.
39.
Dalia I. Hamdan Samia S. Hafez Wafaa H. B. Hassan Mai M. Morsi Heba M. A. Khalil Yasmine H. Ahmed Omar A. Ahmed-Farid Riham A. El-Shiekh 《RSC advances》2022,12(6):3476
This study was conducted to explore the potential cardioprotective and anti-depressive effects of dichloromethane (DCM) fractions of Morus macroura leaves (L) and stem branches (S) on post-myocardial infarction (MI) depression induced by isoprenaline (ISO) in rats in relation to their metabolites. The study was propped with a UPLC-ESI-MS/MS profiling and chromatographic isolation of the secondary metabolites. Column chromatography revealed the isolation of lupeol palmitate (6) that was isolated for the first time from nature with eight known compounds. In addition, more than forty metabolites belonging, mainly to flavonoids, and anthocyanins groups were identified. The rats were injected with ISO (85 mg kg−1, s.c) in the first two days, followed by the administration of M. macroura DCM-L and DCM-S fractions (200 mg kg−1 p.o) for 19 days. Compared with the ISO exposed rats, the treated rats displayed a reduction in cardiac biomarkers (LDH and CKMB), anxiety, and depressive-like behaviour associated with an increase in the brain defense system (SOD and GSH), neuronal cell energy, GABA, serotonin, and dopamine, confirmed by histopathological investigations. In conclusion, DCM-L and DCM-S fractions'' cardioprotective and anti-depressive activities are attributed to their metabolite profile. Therefore, they could serve as a potential agent in amending post-MI depression.This study was conducted to explore the potential cardioprotective and anti-depressive effects of dichloromethane fractions of Morus macroura leaves and stem branches on post-myocardial infarction depression induced by isoprenaline in rats in relation to their metabolites. 相似文献
40.
Ayman El-Garf Samia Salah Iman Iskander Hala Salah Sherif Naseh Amin 《Rheumatology international》2010,30(10):1293-1298
The objective of the study is to screen 12 MEFV gene mutations in Egyptian patients with familial Mediterranean fever (FMF)
and to study the initial hypothesis that the phenotypic expression of the disease may be attributable to the existence of
a particular mutation. We enrolled 136 Egyptian patients (74 males, and 62 females) with a clinical diagnosis of FMF. DNA
was amplified by PCR and subjected to reverse hybridization for the detection of 12 MEFV gene mutations. The phenotypic expression
of the disease was compared in two subgroups according to the presence of homozygote E148Q and M694V gene mutations. The most
frequent gene mutations in the studied group were V726A, M694V, M680I, E148Q and M694I in 41.2, 32.4, 29.4, 25 and 20.6%,
respectively. At least one of these main five founder mutations was present in 132 patients (97.1%). Thirty-two patients (23.5%)
were homozygote for one of the main five founder mutations. The most common homozygote gene mutations were E148Q and M694V,
each in 12 patients (8.8%). Significant increase in abdominal pain and arthritis was found in patients with homozygote M694V
mutation compared to those with E148Q mutation. All patients with amyloidosis had M694V gene mutation. The increased frequency
of V726A gene mutation and the rarity of amyloidosis in this study suggest that Egyptian patients may have a milder form of
FMF compared to other populations. The five main founder mutations account for the vast majority of cases of FMF. M694V gene
mutation may be associated with increased frequency of abdominal pain, arthritis and the presence of amyloidosis. 相似文献