数字化技术在预制唇腭裂术前矫治器中的应用价值

目的研究数字化技术在预制唇腭裂(CPL)术前矫治器中的应用价值。方法选取2013年5月至2018年2月于大连市口腔医院治疗的CPL患儿86例,通过数字化技术进行CPL矫治器制作及佩戴。观察患者矫治器佩戴2年后,患者齿槽段体积变量、角度变量及术后并发症发生情况。结果术后2年,健侧磨牙区、前区、极区均高于术前,患侧磨牙区、尖牙区、极区均高于术前,差异有统计学意义(P<0.05);术后2年,所有患儿CPL健侧齿槽座角、患侧齿槽座角、健侧齿槽前方角均小于术前,健侧与患侧齿槽前方夹角大于术前,差异有统计学意义(P<0.05);术后发生组织皮疹20例(23.26%)、发生黏膜溃疡6例(6.98%),所有患儿均未发生乳牙过早萌出,且患儿呛咳及呕吐情况明显得到改善。结论数字化技术在预制唇腭裂术前矫治器中具有较好的临床疗效,可改善CPL畸形程度及牙槽骨形态,减少复诊时间和治疗次数,值得临床推广应用。     

FGF2的生物信息学分析及其在低氧神经保护中的作用研究*

目的: 观察FGF2在低氧及低氧预适应条件下小鼠海马神经元细胞中的表达变化,探究FGF2是否参与低氧耐受神经保护作用。方法: 使用生物信息学方法进行搜索和鉴定FGF2基因家族成员。在细胞层面建立低氧和低氧预适应模型,通过Western-blot、qRT-PCR检测细胞中FGF2表达情况。结果: FGFs家族分为7个亚家族,其中FGF2含有一个由17个氨基酸组成的FGF受体结合域,能够与特定的FGF受体结合进行信号的转导。在细胞层面,相比于空白对照组(C),低氧组(H)和低氧预适应组(HPC)FGF2在mRNA水平和蛋白水平的表达均有所下降(P＜0.05),而与H组相比,HPC组FGF2 mRNA水平和蛋白水平的表达有所升高(P＜0.05)。结论: FGF参与低氧预适应的神经保护作用。     

Short-Term Outcomes and Influencing Factors After Ankle Fracture Surgery

The present study investigated the factors influencing the early clinical outcomes after ankle fracture surgery. We included 88 patients, who had undergone implant removal surgery at 1 year after ankle fracture surgery, with ankle computed tomographic (CT) scans obtained before ankle fracture surgery and at implant removal available. We collected demographic information, including age, sex, the presence of diabetes mellitus, level of trauma energy, and fracture classification from the medical records. We also recorded the fracture height using the radiographs and CT images. The medial joint space and articular incongruity were assessed on the follow-up radiographs and CT scans. Bone attenuation was measured by placing a circular region of interest around the ankle joint on the preoperative CT image. The postimplant removal outcomes were assessed using 2 functional questionnaires, the American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot scale and Foot and Ankle Outcome Score (FAOS). Significant factors related to the AOFAS ankle-hindfoot scale scores and FAOS were identified through univariate analysis using age, sex, radiographic measurements, and CT findings as explanatory variables, followed by multiple regression analysis. On multiple regression analysis, the total FAOS was independently related to the AO classification (p?=?.003) and Lauge-Hansen classification (p?=?.003). The total AOFAS ankle-hindfoot scale score was related to articular incongruity (p?=?.044). The early clinical outcomes after ankle fracture surgery were affected by involvement of the ankle joint fracture rather than the lateral malleolus fracture height. Female sex and the presence of postoperative articular incongruity correlated with inferior early clinical outcomes.     

An Effective Treatment for Heart Failure Caused by Valvular Heart Diseases: Thoracic Sympathetic Block

Background: The pilot study is designed to investigate the effect of continuous thoracic sympathetic block (TSB) on cardiac function, reconstruction, and hemodynamic parameters in patients with heart failure resulting from valvular heart disease. Method: The cardiac function parameters, including left ventricle ejection fraction (LVEF), left ventricle end-diastole diameter (LVEDD), fractional shortening (FS), and N-terminal prohormone of brain natriuretic peptide (NT-proBNP), were measured in 19 patients before and after TSB treatment. The patients were also classified on the basis of NYHA classification system. Results: 4 weeks of TSB administration improved cardiac function in 18 of 19 patients (94.74%). The patients' LVEF, LVEDD, and NT-proBNP were all improved significantly after treatment. Conclusions: The favorable clinical outcome of TSB administration suggests an alternative treatment for the patients with heart failure caused by valvular dysfunctions.     

29例脑死亡器官移植供体的维护体会

【摘要】 目的 探讨脑死亡器官移植供体在ICU的维护要点。方法〓回顾性分析我科2012年1月~2013年12月间完成的29例脑死亡供体器官捐献资料,分析脑死亡供体器官获取前情况,移植肾、移植肝受者情况及急性肾损伤(AKI)供肾的移植效果,总结脑死亡患者作为潜在供体的维护要点。结果〓维护脑死亡供体29例,共捐献肾脏40个、肝脏27个、心脏4个、角膜21对。6例(15%)移植肾受者发生移植物功能延迟恢复,1例(2.5%)发生急性排斥反应、1例(2.5%)发生髂内动脉假性动脉瘤出血切除移植肾,1例(2.5%)发生重症肺炎术后死亡;AKI供肾与非AKI供肾的受者在术后7天、2月肌酐恢复情况及不良事件发生率比较无明显差异;1例(4%)移植肝受者发生多脏器功能衰竭术后死亡。余受者随访至今均恢复顺利,器官功能良好。结论〓经过ICU内积极管理后的潜在器官移植供体能够获得较高质量的移植器官,有较好的移植效果。     

IL-25 Elicits Innate Lymphoid Cells and Multipotent Progenitor Type 2 Cells That Reduce Renal Ischemic/Reperfusion Injury

IL-25 is an important immune regulator that can promote Th2 immune response-dependent immunity, inflammation, and tissue repair in asthma, intestinal infection, and autoimmune diseases. In this study, we examined the effects of IL-25 in renal ischemic/reperfusion injury (IRI). Treating IRI mice with IL-25 significantly improved renal function and reduced renal injury. Furthermore, IL-25 treatment increased the levels of IL-4, IL-5, and IL-13 in serum and kidney and promoted induction of alternatively activated (M2) macrophages in kidney. Notably, IL-25 treatment also increased the frequency of type 2 innate lymphoid cells (ILC2s) and multipotent progenitor type 2 (MPP^type2) cells in kidney. IL-25–responsive ILC2 and MPP^type2 cells produced greater amounts of Th2 cytokines that associated with the induction of M2 macrophages and suppression of classically activated (M1) macrophages in vitro. Finally, adoptive transfer of ILC2s or MPP^type2 cells not only reduced renal functional and histologic injury in IRI mice but also induced M2 macrophages in kidney. In conclusion, our data identify a mechanism whereby IL-25-elicited ILC2 and MPP^type2 cells regulate macrophage phenotype in kidney and prevent renal IRI.     

Protein tyrosine phosphatase conjugated with a novel transdermal delivery peptide,astrotactin 1–derived peptide recombinant protein tyrosine phosphatase (AP-rPTP), alleviates both atopic dermatitis–like and psoriasis-like dermatitis

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