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81.
Erik Kouba Antonio Lopez-Beltran Rodolfo Montironi Francesco Massari Kun Huang Matteo Santoni 《Expert review of molecular diagnostics》2020,20(2):255-264
ABSTRACTIntroduction: The use of liquid biopsy on the blood from solid malignancies provides a convenient way of detecting actionable mutations, monitoring treatment response, detecting early recurrence and prognosticating outcomes. The aim of this review is to discuss the current status and future direction of serum biomarkers in the clinical management of urinary bladder cancer.Areas covered: This review provides an overview of blood liquid biopsy and bladder cancer using methods of circulating tumors cells, circulating RNA, serum metabolites and cell-free DNA. Recent clinical studies and advances in methodology are emphasized. We performed a literature search using PMC/PubMed with keywords including ‘liquid biopsy’, ‘circulating tumor DNA’, ‘cell-free DNA’, ‘biomarkers’, ‘bladder cancer’ ‘precision medicine’. Additional articles were obtained from the cited references of key articles. An emphasis was placed on recent studies published since 2018.Expert opinion: Liquid biopsies represent a potential biomarker using cell-free DNA, metabolomic profiles of altered cellular metabolism, circulating cancer cells and RNA. Despite displaying tremendous clinical promise, the current status of the blood liquid biopsies has not reached fruition. However, future investigations should lead the evolution of liquid biomarker into clinical utility for the management of bladder cancer. 相似文献
82.
83.
The cytologic features of 46 lymphoproliferative ophthalmic lesions were evaluated using the “squash” technique and/or touch imprints at the time of frozen section. Of the 46 lesions, 33 were located in the orbit, 12 in the conjunctiva, and one in the eyelid. Fifteen cases were benign (reactive) hyperplasias, and 31 were diagnosed as malignant lymphoproliferative tumors. The cytologic features of the reactive group included a polymorphic lymphoid population intermixed with scattered tingible body macrophages. Within the malignant category, there were 28 malignant lymphomas. All 28 cases were diagnosed as non-Hodgkin's lymphomas. Cytologically, the malignant group consisted of a monomorphic population of atypical lymphoid cells. Of the 31 malignant lymphoid lesions, 58% (18 tumors) were small lymphocytic proliferations. The remaining 13 tumors were classified as follows: mixed small/large cell or pure large cell lymphomas (nine), plasmacytoma (three), and Burkitt's lymphoma (one). Final classification using the Working Formulation was made after evaluating the cytologic preparations and surgical material. In selected cases, the monoclonal nature of the malignant lymphoproliferative lesions was confirmed by flow cytometry and/or immunocytochemistry. Cytologic preparations of the fresh specimens preserve the morphologic details of the tumor cells, which is especially important when evaluating lymphoid lesions. Additionally, multiple smears can be prepared simultaneously for ancillary studies such as immunocytochemistry. Of our 46 lymphoproliferative lesions, two-thirds were diagnosed as malignant lymphomas. Based on the morphologic and immunophenotypic findings, all the malignant lymphomas were B-cell tumors, 50% of which were low grade using the Working Formulation. Diagn Cytopathol 1996;15:241–245 © 1996 Wiley-Liss, Inc. 相似文献
84.
Buciuc Marina Whitwell Jennifer L. Boeve Bradley F. Ferman Tanis J. Graff-Radford Jonathan Savica Rodolfo Kantarci Kejal Fields Julie A. Knopman David S. Petersen Ronald C. Parisi Joseph E. Murray Melissa E. Dickson Dennis W. Josephs Keith A. 《Journal of neurology》2020,267(5):1444-1453
Journal of Neurology - Trans-active response DNA-binding protein of 43 kDa (TDP-43) can be detected in up to 63% of autopsy-confirmed Lewy body disease (LBD) cases. It is unclear whether... 相似文献
85.
Mario Amore Cecchini Maria Paula Foss Vitor Tumas Flávia A.P. Patrocinio Rodolfo D. Chiari-Correia Nathalia Novaretti Tamara R. Brozinga Valéria Santoro Bahia Leonardo Cruz de Souza Henrique Cerqueira Guimarães Paulo Caramelli Thais Bento Lima-Silva Luciana Cassimiro Sonia Maria Dozzi Brucki Ricardo Nitrini Sergio Della Sala Mario A. Parra Mônica Sanches Yassuda 《International journal of geriatric psychiatry》2020,35(11):1331-1340
86.
Claudia Espinosa‐García Claudia Erika Fuentes‐Venado Christian Guerra‐Araiza Julia Segura‐Uribe Edwin Chvez‐Gutirrez Eunice Dalet Farfn‐García Norma Anglica Estrada Cruz Rodolfo Pinto‐Almazn 《The European journal of neuroscience》2020,52(1):2724-2736
The metabolic syndrome includes changes in blood glucose levels, arterial hypertension, triglycerides, dyslipidemia and central obesity. Countless reports have described the correlation between the metabolic syndrome and cognitive impairment. However, only a few reports have assessed cognitive impairment associated with the metabolic syndrome in animals of both sexes. For this purpose, Sprague‐Dawley male and female rats were fed either with a hypercaloric diet as model of the metabolic syndrome or with a standard chow diet as controls. Subsequently, spatial learning and memory (Morris water maze) as well as short‐ and long‐term memory (passive avoidance task) were evaluated. Body weight, blood pressure, triglycerides, and total cholesterol significantly increased (F(1, 36) = 94.89, p < .001) in rats fed with hypercaloric diet compared to control rats. Furthermore, cognitive impairment was observed in spatial learning and spatial memory on male rats but not on female rats fed with hypercaloric diet. In addition, a long‐term memory impairment was observed in both groups fed with hypercaloric diet in comparison to their respective control group (F(1, 32) = 10.61, p = .0027). Immunohistochemistry results showed no changes in the number of positive cells for NeuN, GFAP and Ox‐42. In males fed with a hypercaloric diet, a decrease in testosterone levels was observed, whereas estradiol levels decreased in females when compared with their respective control group (p < .0001). In this MetS animal model, metabolic and cognitive differences were observed in males and females, which demonstrates that sex hormones play a significant role in metabolic regulation and neuroprotection related to the CA1 region of the hippocampus. 相似文献
87.
Reading errors in patients with cerebellar vermis lesions 总被引:2,自引:0,他引:2
Dyslexia, both developmental and acquired, has been considered the result of cerebrocortical dysfunction, affecting the temporo-parieto-occipital
brain regions. However, dyslexia may involve abnormalities of the magnocellular component of the visual system, leading to
binocular instability or alterations of accommodation. To test the hypothesis of cerebellar involvement in the reading process
– justified by its emergent role in language and cognition – we studied 10 patients with cerebellar vermis/paravermis lesions
using reading tests and we compared the results with those produced by 10 normal volunteers. The data obtained demonstrate
an increased number of reading mistakes in the patient group, resulting from a possible alteration of the diffuse connection
system from the cerebellum to different cerebrocortical and subcortical structures. Acquired dyslexia due to cerebellar impairment
may be due to oculomotor alteration or, more subtly, to the intimate cerebellar-encephalic projections, connecting the cerebellum
to the attentive and alerting processes and to the language system. We discuss the data with an overview of literature.
Received: 22 May 2001, Received in revised form: 14 September 2001, Accepted: 25 September 2001 相似文献
88.
Ringer Ariana Ruffino Juan Pablo Leiva Rodolfo Cuadranti Nadia Argento María Cecilia Martínez María Florencia Rolla Ignacio Chulibert Serenela Carbone Daniela Palatnik Mariano Cortese Maria Noel Lagrutta Mariana Córdoba Laura González Florencia Belén Pacini María Florencia Villar Silvina Raquel Águila Damian Bottasso Oscar Adelmo Pérez Ana Rosa Abdala Marcelo 《Clinical rheumatology》2021,40(7):2955-2963
Clinical Rheumatology - Evidence for Chagas disease reactivation (CDR) in rheumatologic patients under rheumatologic treatments (RTs) is scarce. To screen and follow-up patients with rheumatic... 相似文献
89.
Ruben Marroquin-Segura Maurilio Flores-Pimentel Rodolfo Carreón-Sánchez Martha M. Garcia-Burciaga José Luis Alfredo Mora-Guevara Abigail Aguilar-Contreras Vicente Jesús Hernandez-Abad 《Journal of ethnopharmacology》2009
Aims of study
Despite the ethnopharmacological relevance of Helietta parvifolia A. Gray (Rutaceae) in Mexico, we found no significant pharmacological studies of this plant in the scientific literature. The aim of the present study was to establish the anti-inflammatory effect of an aqueous extract of the stem bark of Helietta parvifolia in mice.Materials and methods
The anti-inflammatory activity of the aqueous extract of the stem bark of Helietta parvifolia was evaluated using carrageenan-induced paw oedema in mice, and the cotton pellet granuloma method.Results
An extract dose ranging from 20 to 80 mg/kg p.o. showed a non-significant effect over the initial phase of carrageenan-induced oedema. However, it showed a significant inhibition of oedema after 3 h, which can be related to the inhibition of the release of kinin-like substances. An ID50 value of 47.4 mg/kg was obtained for the plant extract. The extract also suppressed granulomatous tissue formation during chronic inflammation. The inhibitory values were 19.2, and 22.2, corresponding to 40 and 80 mg/kg doses of extract respectively.Conclusions
Aqueous extract showed a statistically significant anti-inflammatory effect in mice during the late phase of acute inflammation and during chronic inflammation. However, the exact mechanism(s) of anti-inflammatory effects of Helietta parvifolia observed in this study remains unclear. 相似文献90.
Melanoma risk alleles are associated with downregulation of the MTAP gene and hypermethylation of a CpG island upstream of the gene in dermal fibroblasts 下载免费PDF全文
Antonella Sangalli Giovanni Malerba Gianpaolo Tessari Monica Rodolfo Macarena Gomez‐Lira 《Experimental dermatology》2017,26(8):733-736
Several association studies and GWAS on melanoma skin cancer risk have reported statistically significant signals on 9p21.3 region, where MTAP gene maps. None of the associated SNPs identified in these studies lie in the coding region of the gene and the causative relation of risk alleles with melanoma predisposition has not been elucidated. MTAP has a tumor suppressor activity and epigenetic silencing has been described in melanoma cell lines. In the present study, we show that melanoma risk alleles correlate with a MTAP allele‐specific hyper‐methylation and down‐regulation of gene expression. 相似文献