大豆提取物(CKBN)对免疫低下小鼠免疫功能的影响

目的观察大豆提取物（CKBN）对免疫低下小鼠免疫功能的影响。方法腹腔注射环磷酰胺（cyclophosphamide,CTX）建立免疫功能低下小鼠模型,观察1、25、50、100 mg/kg剂量CKBN对免疫低下小鼠免疫功能的影响。结果25 mg/kg组的CKBN可显著增加免疫低下小鼠的脾指数;25、50、100 mg/kg组均能明显抑制环磷酰胺对小鼠外周血白细胞数量的影响,1 mg/kg组可显著提高单核细胞百分率,50 mg/kg组可显著提高中性粒细胞百分率;100 mg/kg组的IgG2a水平高于环磷酰胺组;1、25、50 mg/kg可显著提高腹腔巨噬细胞的吞噬功能;25 mg/kg组可显著提高NK细胞杀伤活性。结论CKBN能显著增强CTX造成的免疫低下小鼠的免疫功能。     

Slug (SNAI2) down-regulation by RNA interference facilitates apoptosis and inhibits invasive growth in neuroblastoma preclinical models

PURPOSE: We assessed the relevance of Slug (SNAI2) for apoptosis resistance and invasion potential of neuroblastoma cells in vitro and in vivo. EXPERIMENTAL DESIGN: We evaluated the effect of imatinib mesylate on invasion and analyzed the genes modulated by imatinib mesylate treatment in neuroblastoma cells. Slug expression, inhibited by imatinib mesylate treatment, was knocked down in neuroblastoma cells by RNA interference, and the effects on invasion and apoptosis were evaluated in vitro. A pseudometastatic model of neuroblastoma in severe combined immunodeficient mice was used to assess the effects of Slug silencing alone or in combination with imatinib mesylate treatment on metastasis development. RESULTS: Microarray analysis revealed that several genes, including Slug, were down-regulated by imatinib mesylate. Slug expression was detectable in 8 of 10 human neuroblastoma cell lines. Two Slug-expressing cell lines were infected with a vector encoding a microRNA to Slug mRNA. Infected cells with reduced levels of Slug were tested for the expression of apoptosis-related genes (p53, Bax, and Bcl-2) identified previously as Slug targets. Bcl-2 was down-regulated in Slug-interfered cells. Slug down-regulation increased sensitivity to apoptosis induced by imatinib mesylate, etoposide, or doxorubicin. Invasion of Slug-silenced cells was reduced in vitro. Animals injected with Slug-silenced cells had fewer tumors than controls and the inhibition of tumor growth was even higher in animals treated with imatinib mesylate. CONCLUSIONS: Slug down-regulation facilitates apoptosis induced by proapoptotic drugs in neuroblastoma cells and decreases their invasion capability in vitro and in vivo. Slug inhibition, possibly combined with imatinib mesylate, may represent a novel strategy for treatment of metastatic neuroblastoma.     

The Expression of RECK mRNA and Protein in Esophageal Squamous Cell Carcinoma and Its Clinical Significance

目的:探讨食管鳞癌组织中RECK mRNA和蛋白的表达情况及其与临床病理因素的关系。方法:应用RT-PCR和免疫组化SP法检测62例食管鳞癌组织、31例癌旁不典型增生组织及62例正常食管粘膜组织中mRNA和蛋白的表达。结果:在食管鳞癌癌变过程中RECK在癌组织、癌旁不典型增生组织及正常粘膜组织中mRNA的含量依次增高,分别为1.052±0.078、1.274±0.235、1.306±0.121,组间比较有明显差异(F=49.936,P<0.05);不同分化程度、不同浸润深度及有无淋巴结转移的食管鳞癌组织之间RECK mRNA相对含量差异均有统计学意义(F=5.081,F=26.084,U=24.011,P均<0.05)。食管鳞癌组织及癌旁不典型增生组织中RECK蛋白表达均低于正常粘膜组织,表达量分别为59.7%(37/62)、71.0%(22/31)、85.5%(53/62),组间比较有明显差异(χ2=10.331,P<0.01);食管鳞癌组织中RECK蛋白表达与癌组织的分化程度、不同浸润深度及有无淋巴结转移密切相关(P<0.05)。结论:食管鳞癌组织中RECK mRNA和蛋白表达均降低,其低表达可能与食管鳞癌发生有关。检测RECK mRNA及蛋白的表达可望成为食管鳞癌早期诊断和判断预后的分子指标之一。     

番茄红素油树脂挥发性化学成分研究

为了研究番茄红素油树脂的可挥发性成分及脂溶性成分,利用气相色谱-质谱GC-MS连用技术对番茄红素油树脂的挥发性成分及部分可皂化成分和非皂化成分进行了分析.根据经质谱数据库检索、人工分析并与标准图谱核对,鉴定19种挥发性成分,17种可皂化成分和12种非皂化成分,为了进一步开发天然保健食品及药品的功能因子番茄红素和提高番茄红素油树脂的品质提供了一定的科学依据.     

腹腔镜技术在中晚期妊娠合并阑尾炎中的应用

目的探讨腹腔镜手术在中晚期妊娠合并急性阑尾炎中的应用价值。方法对我院2006年1月至2007年12月间19例中、晚期妊娠合并阑尾炎采用经腹腔镜下阑尾切除术的患者进行回顾性分析。结果19例均顺利完成手术,无1例中转开腹。均痊愈出院,无流产、子宫损伤、腹腔脏器损伤、切口感染裂开、腹腔感染、腹腔残余脓肿形成、肠梗阻等并发症出现。结论中晚期妊娠合并急性阑尾炎行腹腔镜手术有微创,美容,探查全面,降低阴性剖腹率,对急性阑尾炎能早期诊断早期手术,减少阑尾穿孔的机会,减少对子宫的刺激,减少流产的机率。并发症少,缩短住院日期等优点。     

Potential involvement of mt1 receptor and attenuated sex steroid-induced calcium influx in the direct anti-proliferative action of melatonin on androgen-responsive LNCaP human prostate cancer cells

Melatonin, a pineal secretory product, has been shown to exert a direct anti-proliferative action on the androgen-sensitive LNCaP prostate cancer cell line through hitherto undefined mechanisms. In this communication, expression of mt1 melatonin receptor protein in human prostate cancer tissues and LNCaP cells was demonstrated by immunohisto(cyto)chemistry and western blotting, hence supporting the use of LNCaP cell line as a model for the study of melatonin signaling in prostate cancer cell growth. Using 3H-thymidine incorporation assay, LNCaP cell proliferation was inhibited by 2-iodomelatonin, a high-affinity melatonin receptor agonist. Furthermore, melatonin inhibited 3H-thymidine incorporation into LNCaP cells and attenuated 5alpha-dihydrotestosterone (DHT) or 17beta-estradiol (E2)-induced stimulation of LNCaP cell proliferation at physiological and pharmacological concentrations. Similar concentration-dependent inhibition of sex steroid-induced stimulation of thymidine incorporation into LNCaP cells by 2-iodomelatonin was also observed. Interestingly, attenuation of sex steroid-stimulated calcium influx into LNCaP cells by pharmacological concentrations of melatonin was recorded, whereas 2-iodomelatonin had no effect on cytosolic calcium changes induced by sex steroids. In addition, proliferative and cytosolic calcium changes were associated with inhibition of total prostate-specific antigen (PSA) production by LNCaP cells at high physiological and pharmacological concentrations of melatonin. Our data suggest that activated mt1 receptor and attenuated sex steroid-induced calcium influx are two important mechanisms mediating the direct anti-proliferative action of melatonin on androgen-responsive human prostate cancer cells.     

Clinical study on kidney-nourishing and governor vessel-regulating acupuncture therapy for apoplexy sequela

目的：观察益肾调督针法治疗中风后遗症的疗效。方法：60例中风后遗症患者随机分为益肾调督取穴组和普通取穴组各30例，治疗35d。观察针剌后生存质量测定积分，血清白介素-6（IL-6）、白介素-8（IL-8）、肿瘤坏死因子（TNF）的水平，并比较临床疗效。结果与结论：两组针法治疗前后各项指标差别有显著性，两组针法治疗中风60例均有效。两组针法间比较，益肾调督取穴组优于普通取穴组。     

吉林省农村厕所及粪便处理现状调查

本文对吉林省农村厕所及粪便处理现状进行了调查。结果表明，本省户厕主要以浅坑为主（１．５ｍ以上），占９５％以上，深坑与浅坑的卫生学指标有明显差异。厕所７５．７２％有渗漏现象，仅３．３％，能进行无害化处理。     

疾病社会负担测量方法探讨及其意义

目的：探讨疾病的社会负担对总疾病负担的影响。方法 以脑卒中为例,用失能调整寿命年指标对其疾病负担进行测量,并以住院患的陪护情况为代表尝试测量其部分社会负担。结果 在所研究人群中,以每千人失能调整寿命年表示的脑卒中患本人的疾病负担,男性和女性分别为１１．７０和９．３８,而患住院期间的陪护负担分别相当于其本身疾病负担的２．２％和２．８％。结论 疾病负担测量中应考虑社会负担的影响。