Seizures induced by frustration and despair due to unresolved moral and political issues: a rare case of reflex epilepsy

We present a case of reflex-induced simple partial seizures, triggered by feelings of frustration, anger and despair. Such emotions were provoked by pondering over complex national and international, political and moral issues. The present case may suggest that activation of right temporal networks may mediate negative and adverse emotions induced by preoccupation with agitating, controversial issues.     

紫衫醇与顺铂诱导卵巢癌SKOV_3细胞凋亡的研究

目的 :研究两种不同的抗癌药物诱导细胞凋亡的规律 ,为卵巢癌的化疗提供理论依据。方法 :紫衫醇与顺铂分别作用于体外培养的卵巢癌SKOV3 细胞系 ,通过组织细胞化学染色、荧光染色、电子显微镜、DNA凝胶电泳、流式细胞术等方法进行检测和观察。结果 :经两种抗癌药物处理的卵巢癌SKOV3 细胞出现典型的细胞凋亡特征 ,在顺铂处理 0～ 36小时内 ,细胞凋亡呈均一的持续性增强 ,在 5～ 2 5nmol ml剂量范围内呈依赖性增强 ,凋亡率最高达 72 %。紫衫醇诱导细胞凋亡亦具有时间和剂量依赖性 ,2 5nmol ml浓度处理 12小时 ,凋亡率最高达 5 5 % ;但时间过长 (>12小时 )或浓度过大 (>5 0nmol ml) ,凋亡率下降。结论 :紫衫醇与顺铂能有效地诱导肿瘤细胞凋亡 ,诱导细胞凋亡是化疗药物治疗癌症的重要机制之一     

‘Shadowing’ as a management strategy for chemotherapy outpatient primary support persons

Despite growing numbers of patients with cancer receiving chemotherapy in outpatient settings and the corresponding increase in care demands on family and close friends, little is known about the experiences of those informal carers supporting people with cancer during their chemotherapy trajectories. Using an interpretivist theoretical framework, this study explored the experiences of primary support persons of chemotherapy outpatients through in‐depth interviews with 17 participants nominated as their primary support persons by people receiving chemotherapy at a large tertiary hospital in Australia. The study demonstrates that primary support persons of chemotherapy outpatients face distinct challenges, being at the frontline of treatment and managing side effects with minimal support at home. This role involves sensitive provision of complex medical and social care in circumstances that profoundly challenge the everyday worlds of both patient and carer. From the moment of diagnosis, informal carers in this context face the ‘double whammy’ of needing to ‘manage’ the cancer diagnosis experience as well as the chemotherapy trajectory experience. This study points to the significant level of responsibility that primary support persons take on, and the extent to which patients and clinicians rely on their support and management skills. It also points, however, to the lack of recognition they receive for assuming this role, and their sense of frustration in the face of this invisibility. The conceptualisation of the informal carer role as a ‘shadowing’ role explicitly represents the protective, vigilant, but almost invisible, support role described by the participants in this study.     

Microsurgery of the upper extremity

In the past 50 years, hand surgeons have made considerable contributions to microsurgery. The unique demands of complex upper extremity care have driven many of the technical and scientific advances of this discipline, including functional muscle transfers, nerve transfers, and composite tissue allotransplantation. The purpose of this article was to review the current applications of microsurgery to the upper extremity.     

迭代重建法与滤过反投影重建法冠状动脉CTA对严重钙化血管评估的对照研究

目的前瞻性研究冠状动脉CT血管成像(CTA)中滤过反投影图像重建(FBP)法和迭代图像重建法对严重钙化的冠状动脉评估的价值。材料与方法此研究获机构审查委     

DNA-labelled cytidine assay for the quantification of CAG repeats

The sequencing procedure has been used to determine the size of the CAG repeat expansion for the diagnosis of genetic disorders. Likewise, standard polymerase chain reaction (PCR) and gel electrophoresis techniques are applied for screening large number of patients. The trinucleotide repeats (TNR) region amplification by means of the PCR procedure was initially performed using 32-P end-labelled primers and currently carried out with fluorescently end-labelled primers. The goal to obtain reliable TNR quantification assays, at low cost and short assay times, represents a challenge for the molecular diagnosis aimed at massive screening of affected populations. In the current work, we obtained preliminary results of a new methodology for the detection and size estimation of CAG expanded alleles. The assay was based on an indirect enzyme linked immunosorbent assay (ELISA) for quantifying the amount of labelled cytidines in DNA molecules. The label, 6-(p-bromobenzamido)caproyl radical, was introduced by the transamination and acylation reactions. A group of model sequences containing different numbers of CAG repeats, as well as the ATXN3 (ataxin 3) gene (from subjects suffering type 3 spinocerebellar ataxia SCA3) were used for assay standardization. The assay is simple, inexpensive, and easy to perform and differentiates distinct degrees of CAG expansions.     

Wearables assist in the development of self-acute status assessmentapps北大核心CSCD

[No abstract available]