全文获取类型
收费全文 | 3036篇 |
免费 | 163篇 |
国内免费 | 33篇 |
专业分类
耳鼻咽喉 | 8篇 |
儿科学 | 107篇 |
妇产科学 | 47篇 |
基础医学 | 546篇 |
口腔科学 | 82篇 |
临床医学 | 399篇 |
内科学 | 664篇 |
皮肤病学 | 122篇 |
神经病学 | 195篇 |
特种医学 | 286篇 |
外科学 | 263篇 |
综合类 | 56篇 |
预防医学 | 141篇 |
眼科学 | 34篇 |
药学 | 216篇 |
中国医学 | 3篇 |
肿瘤学 | 63篇 |
出版年
2018年 | 32篇 |
2015年 | 40篇 |
2014年 | 41篇 |
2013年 | 111篇 |
2012年 | 73篇 |
2011年 | 57篇 |
2010年 | 64篇 |
2009年 | 53篇 |
2008年 | 67篇 |
2007年 | 90篇 |
2006年 | 92篇 |
2005年 | 57篇 |
2004年 | 49篇 |
2003年 | 60篇 |
2002年 | 46篇 |
2001年 | 53篇 |
2000年 | 43篇 |
1999年 | 51篇 |
1998年 | 86篇 |
1997年 | 97篇 |
1996年 | 110篇 |
1995年 | 77篇 |
1994年 | 79篇 |
1993年 | 88篇 |
1992年 | 59篇 |
1991年 | 60篇 |
1990年 | 62篇 |
1989年 | 95篇 |
1988年 | 82篇 |
1987年 | 74篇 |
1986年 | 75篇 |
1985年 | 59篇 |
1984年 | 66篇 |
1983年 | 35篇 |
1982年 | 35篇 |
1981年 | 45篇 |
1980年 | 53篇 |
1979年 | 36篇 |
1978年 | 29篇 |
1977年 | 37篇 |
1976年 | 45篇 |
1975年 | 41篇 |
1972年 | 30篇 |
1971年 | 28篇 |
1965年 | 50篇 |
1964年 | 51篇 |
1963年 | 47篇 |
1962年 | 41篇 |
1961年 | 40篇 |
1960年 | 65篇 |
排序方式: 共有3232条查询结果,搜索用时 15 毫秒
91.
恰如其分的外周髓鞘形成取决于雪旺细胞增殖与分化进程间的平衡。丝氨酸/苏氨酸激酶(mTOR)整合多种环境因素,是细胞生长、代谢、发挥作用的中枢调节者。本文报道了一种mTOR的负性调节剂——结节性硬化复合体(TSC1),通过控制细胞增殖和髓鞘稳态,建立了雪旺细胞谱系进展和髓鞘形成的阶段依赖性程序。小鼠雪旺细胞祖细胞中TSC1的解离导致mTOR信号通路激活,继而导致雪旺细胞过量增殖,分化受阻,髓鞘形成减少。转录组分析显示,TSC1突变体中的mTOR活化使得polo样激酶(PLK)依赖性通路和细胞周期调节剂上调。弱化mTOR或者对PLK进行药理抑制部分挽救了因TSC1缺失导致的外周神经发育过程中的髓鞘形成减少。相较之下,成年小鼠成熟雪旺细胞中TSC1缺失可导致髓鞘的过度增殖和过度生长。本文的发现提示了TSC1-mTOR-PLK信号轴在控制雪旺细胞的发育过程中,从增殖到分化和髓鞘内稳态中起到的阶段特异性功能。 相似文献
92.
G R Nakamura R Byrn K Rosenthal J P Porter M R Hobbs L Riddle D J Eastman D Dowbenko T Gregory B M Fendly 《AIDS research and human retroviruses》1992,8(11):1875-1885
Ten monoclonal antibodies prepared against a soluble, recombinant form of gp160, derived from the IIIB isolate of HIV-1, were characterized. Four of the antibodies neutralized HIV-1IIIB infectivity in vitro, three blocked the binding of recombinant gp120 to CD4, three were reactive with gp41, and one preferentially reacted with an epitope on gp120 within the gp160 precursor. All three CD4 blocking antibodies bound to distinct epitopes, with one mapping to the C1 domain, one mapping to the C4 domain, and one reactive with a conformation-dependent, discontinuous epitope. Of these, the antibody reactive with the discontinuous epitope exhibited neutralizing activity against homologous and heterologous strains of HIV-1. The binding of these monoclonal antibodies to a panel of seven recombinant gp120s prepared from diverse isolates of HIV-1 was measured, and monoclonal antibodies with broad cross reactivity were identified. The epitopes recognized by 7 of the 10 monoclonal antibodies studied were localized by their reactivity with synthetic peptides and with fragments of gp120 expressed as fusion proteins in a lambda gt-11 gp160 epitope library. 相似文献
93.
Association between binge eating and attention‐deficit/hyperactivity disorder in two pediatric community mental health clinics 下载免费PDF全文
94.
95.
96.
Eckart RE Love SS Atwood JE Arness MK Cassimatis DC Campbell CL Boyd SY Murphy JG Swerdlow DL Collins LC Riddle JR Tornberg DN Grabenstein JD Engler RJ;Department of Defense Smallpox Vaccination Clinical Evaluation Team 《Journal of the American College of Cardiology》2004,44(1):201-205
OBJECTIVES: The purpose of this study was to assess the follow-up of patients with vaccinia-associated myocarditis. BACKGROUND: With the threat of biological warfare, the U.S. Department of Defense resumed a program for widespread smallpox vaccinations on December 13, 2002. One-year afterwards, there has been a significant increase in the occurrence of myocarditis and pericarditis among those vaccinated. METHODS: Cases were identified through sentinel reporting to military headquarters, systematic surveillance, and spontaneous reports. RESULTS: A total of 540,824 military personnel were vaccinated with a New York City Board of Health strain of vaccinia from December 2002 through December 2003. Of these, 67 developed myopericarditis at 10.4 +/- 3.6 days after vaccination. The ST-segment elevation was noted in 57%, mean troponin on admission was 11.3+/- 22.7 ng/dl, and peak cardiac enzymes were noted within 8 h of presentation. On follow-up of 64 patients (96%) at a mean of 32 +/- 16 weeks, all patients had objective normalization of echocardiography, electrocardiography, laboratory testing, graded exercise testing, and functional status; 8 (13%) reported atypical, non-limiting persistent chest discomfort. CONCLUSIONS: Post-vaccinial myopericarditis should be considered in patients with chest pain within 30 days after smallpox vaccination. Normalization of echocardiography, electrocardiography, and treadmill testing is expected, and nearly all patients have resolution of chest pain on follow-up. 相似文献
97.
NA Hanchard DR Murdock PL Magoulas M Bainbridge D Muzny YQ Wu M Wang AL McGuire JR Lupski RA Gibbs CW Brown 《Clinical genetics》2013,83(5):457-461
The advent of whole‐exome next‐generation sequencing (WES) has been pivotal for the molecular characterization of Mendelian disease; however, the clinical applicability of WES has remained relatively unexplored. We describe our exploration of WES as a diagnostic tool in a 3½‐year old female patient with a 2‐year history of episodic muscle weakness and paroxysmal dystonia who presented following a previous extensive but unrevealing diagnostic work‐up. WES was performed on the proband and her two parents. Parental exome data was used to filter potential de novo genomic events in the proband and suspected variants were confirmed using di‐deoxy sequencing. WES revealed a de novo non‐synonymous mutation in exon 21 of the calcium channel gene CACNA1S that has been previously reported in a single patient as a rare cause of atypical hypokalemic periodic paralysis. This was unexpected, as the proband's original differential diagnosis had included hypokalemic periodic paralysis, but clinical and laboratory features were equivocal, and standard clinical molecular testing for hypokalemic periodic paralysis and related disorders was negative. This report highlights the potential diagnostic utility of WES in clinical practice, with implications for the approach to similar diagnostic dilemmas in the future. 相似文献
98.
A. A. Belogurov JR. T. A. Zargarova V. I. Turobov N. I. Novikova O. O. Favorova N. A. Ponomarenko 《Autoimmunity》2013,46(4):362-364
Previously, we demonstrated that autoantibodies (AAb) in multiple sclerosis (MS) reveal site-specific binding and cleavage toward myelin basic protein (MBP) epitope library. We have found several fragments of MBP immunodominant in terms of AAb binding. Here, we applied these peptides to DA rats with induced protracted relapsing experimental allergic encephalomyelitis (EAE) most closely related to MS. DA rats with EAE induced by syngenic spinal cord homogenate in complete Freund's adjuvant were treated by nasal route with human MBP 46–62, 81–102, 124–139, 147–170, and Copaxone®. MBP 124–139 and 147–170 displayed only mild therapeutic effects but MBP 46–62 significantly reduced EAE, reflected by lower clinical scores and shorter EAE duration compared to controls. 相似文献
99.
Michelle M. Adams M. Elizabeth Forbes M. Constance Linville David R. Riddle William E. Sonntag Judy K. Brunso-Bechtold 《Growth factors (Chur, Switzerland)》2013,31(3):181-188
Insulin-like growth factor-I (IGF-I), a functionally important neurotrophic factor, impacts tissues throughout the body including the central nervous system. In addition to the significant proportion of IGF-I that is synthesized in the liver and released into the plasma, IGF-I is expressed locally in tissues. The present study investigated the relationship between plasma and local brain levels of IGF-I in two well-characterized models of decreased IGF-I. The first is an adult-onset growth hormone deficiency (AOGHD) model, and the second is a caloric restriction (CR) model. In the first cohort of animals from both models, the hippocampus was removed from the brain immediately following decapitation, and in the second cohort, the animals were perfused transcardially with phosphate buffered saline to remove cerebral blood prior to harvesting the hippocampus. Our results demonstrated that although the plasma IGF-I levels were decreased in the CR and AOGHD rats compared to controls, the hippocampal IGF-I levels did not differ among the groups. These data suggest that local brain IGF-I levels are regulated in a different manner than plasma IGF-I levels. 相似文献
100.
Daniel L. Riddle Robert A. Perera William T. Nay Levent Dumenci 《Clinical orthopaedics and related research》2015,473(11):3527-3534