排序方式: 共有37条查询结果,搜索用时 15 毫秒
11.
Flora Suzane Parente Maia Jos Elivalto Guimares Campelo Jos Lindemberg Rocha Sarmento Caio Santos Silva Jos Ribamar Felipe Marques Francisco Arimatia Santos Alves Rafaelle Casseb Guimares Ednaldo Silva Filho 《Parasite immunology》2019,41(4)
This study's purpose was to identify polymorphisms (SNP) in the goat β‐defensin 1 gene and to associate these SNPs with traits related to nematodean and protozoan infections in Anglo‐Nubian goats from semiarid region of Brazil. A total of 184 animals were used for DNA extraction, PCR and DNA automatic sequencing. The association analyses included the fixed effects of animal age, bloodline and genotype of the SNP marker in the statistical model. The means of genotypes were compared by the Fisher test (P < 0.05). Twelve polymorphism genotypes were found: two in intron 1, seven in exon 2 and three in 3′ untranslated region. The SNPs of exon 2 were responsible for amino acid substitutions in six genetic codes, and the changes in the 25th and 33rd codes affected the protein function. The SNP 1937 was significantly associated with number of protozoan oocysts, whereas SNP 2001 was associated with degree of anaemia (Famacha©). Polymorphism 2046, in turn, showed a significant association with Famacha© degree and number of protozoan oocysts. SNP 2140 associated with maximum EPG of the animal. Results from this study suggest that the β‐defensin 1 gene can be used as a molecular marker for selection of goats regarding the susceptibility to endoparasites infections. 相似文献
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Leonardo Emberti Gialloreti Monica Merito Patrizio Pezzotti Luigi Naldi Antonio Gatti Maud Beillat Laurence Serradell Rafaelle di Marzo Antonio Volpi 《BMC infectious diseases》2010,10(1):230
Background
Data on the epidemiology and cost of herpes zoster (HZ) and post-herpetic neuralgia (PHN) in Italy are limited. This retrospective, population-based study was designed to determine the incidence of HZ and the proportion developing PHN in Italy and the associated medical resource utilisation and costs. It focused primarily on immunocompetent patients aged ≥50 years who would be eligible for preventive vaccination. 相似文献14.
Miot HA Batistella RF Batista Kde A Volpato DE Augusto LS Madeira NG Haddad V Miot LD 《Revista do Instituto de Medicina Tropical de S?o Paulo》2004,46(5):253-256
DEET (N,N-diethyl-3-methylbenzamide) is nowadays the most effective mosquito repellent available, however, its use can present some topical and systemic side effects. Some botanical compositions, as Andiroba (Carapa guianensis), have been proved repellent properties at low cost and toxicity. An experimental study was driven involving four volunteers submitting their forearms covered with Andiroba oil at 100%, DEET 50%, refined soy oil, Andiroba oil 15% and in the absence of products, directly to healthy females of Aedes sp. The times of first and third bites were checked. The results showed that the median of the first bite without any product was 17.5s and the third bite, 40.0s. In the soy oil, the bites happened in 60.0s and 101.5s, in the presence of Andiroba oil 100%, in 56.0s and 142.5s and in Andiroba oil 15%, in 63.0s and 97.5s. The volunteers using DEET 50% had not received bites after 3600s in most of the experiments (p < 0.001 Wilcoxon). Pure Andiroba oil compared to the soy oil, forearm without product and Andiroba oil 15%, showed discreet superiority (p < 0.001 Wilcoxon). Our conclusion is that this study demonstrated that the pure Andiroba oil presents discreet repellent effect against bite of Aedes sp., being significantly inferior to DEET 50%. 相似文献
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Dimitri Renard Guillaume Taieb Matteo Garibaldi Andre Maues De Paula Rafaelle Bernard Nadira Lagha Gael Cristofari Catherine Vovan Charlène Chaix Nicolas Lévy Philippe Khau Van Kien Sabrina Sacconi 《American journal of medical genetics. Part A》2018,176(8):1760-1763
Facioscapulohumeral muscular dystrophy (FSHD) has been shown to be related to genetic and epigenetic derepression of DUX4 (mapping to chromosome 4), a gene located within a repeat array of D4Z4 sequences of polymorphic length. FSHD type 1 (FSHD1) is associated with pathogenic D4Z4 repeat array contraction, while FSHD type 2 (FSHD2) is associated with SMCHD1 variants (a chromatin modifier gene that maps to the short arm of chromosome 18). Both FSHD types require permissive polyadenylation signal (4qA) downstream of the D4Z4 array. 相似文献
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Vasli N Böhm J Le Gras S Muller J Pizot C Jost B Echaniz-Laguna A Laugel V Tranchant C Bernard R Plewniak F Vicaire S Levy N Chelly J Mandel JL Biancalana V Laporte J 《Acta neuropathologica》2012,124(2):273-283
Inherited neuromuscular disorders (NMD) are chronic genetic diseases posing a significant burden on patients and the health
care system. Despite tremendous research and clinical efforts, the molecular causes remain unknown for nearly half of the
patients, due to genetic heterogeneity and conventional molecular diagnosis based on a gene-by-gene approach. We aimed to
test next generation sequencing (NGS) as an efficient and cost-effective strategy to accelerate patient diagnosis. We designed
a capture library to target the coding and splice site sequences of all known NMD genes and used NGS and DNA multiplexing
to retrieve the pathogenic mutations in patients with heterogeneous NMD with or without known mutations. We retrieved all
known mutations, including point mutations and small indels, intronic and exonic mutations, and a large deletion in a patient
with Duchenne muscular dystrophy, validating the sensitivity and reproducibility of this strategy on a heterogeneous subset
of NMD with different genetic inheritance. Most pathogenic mutations were ranked on top in our blind bioinformatic pipeline.
Following the same strategy, we characterized probable TTN, RYR1 and COL6A3 mutations in several patients without previous molecular diagnosis. The cost was less than conventional testing for a single
large gene. With appropriate adaptations, this strategy could be implemented into a routine genetic diagnosis set-up as a
first screening approach to detect most kind of mutations, potentially before the need of more invasive and specific clinical
investigations. An earlier genetic diagnosis should provide improved disease management and higher quality genetic counseling,
and ease access to therapy or inclusion into therapeutic trials. 相似文献
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Maryvonne Ardourel Chlo Felgerolle Arnaud Pris Niyazi Acar Khaoula Ramchani Ben Othman Natsuko Ueda Rafaelle Rossignol Audrey Bazinet Betty Hbert Sylvain Briault Isabelle Ranchon-Cole Olivier Perche 《Nutrients》2021,13(9)
To prevent ocular pathologies, new generation of dietary supplements have been commercially available. They consist of nutritional supplement mixing components known to provide antioxidative properties, such as unsaturated fatty acid, resveratrol or flavonoids. However, to date, only one preclinical study has evaluated the impact of a mixture mainly composed of those components (Nutrof Total®) on the retina and demonstrated that in vivo supplementation prevents the retina from structural and functional injuries induced by light. Considering the crucial role played by the glial Müller cells in the retina, particularly to regulate the glutamate cycle to prevent damage in oxidative stress conditions, we questioned the impact of this ocular supplement on the glutamate metabolic cycle. To this end, various molecular aspects associated with the glutamate/glutamine metabolism cycle in Müller cells were investigated on primary Müller cells cultures incubated, or not, with the commercially mix supplement before being subjected, or not, to oxidative conditions. Our results demonstrated that in vitro supplementation provides guidance of the glutamate/glutamine cycle in favor of glutamine synthesis. These results suggest that glutamine synthesis is a crucial cellular process of retinal protection against oxidative damages and could be a key step in the previous in vivo beneficial results provided by the dietary supplementation. 相似文献
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Blandine Maurel Tim Resch Rafaelle Spear Blayne Roeder Umberto M. Bracale Stephan Haulon Tara M. Mastracci 《Journal of vascular surgery》2017,65(4):972-980
Objective
Preloaded endovascular delivery systems expand the anatomic eligibility for complex aortic repair by requiring only one iliac access vessel and providing a stable platform for guiding sheaths into challenging target vessels. This article reports the lessons learned and early clinical outcomes using a modified preloaded delivery system for fenestrated endovascular aneurysm repair (FEVAR) in three aortic centers in Europe.Methods
From October 2015 to March 2016, consecutive patients presenting with extensive aortic aneurysm treated with a modified preloaded FEVAR were prospectively enrolled from three high volume European aortic centers. The new design is a modification of previous designs of preloaded fenestrated stent grafts and of the p-branch device platform. The technical details of implantation are described and perioperative outcomes, including the learning curve, are collected and reported.Results
All patients (30 patients; 80% men; 70.2 years old) presented for nonurgent repair of either a type Ia endoleak (3/30; 10%), a type I-II-III thoracoabdominal (8/30; 27%), or a type IV thoracoabdominal or pararenal (19/30; 63.%) aneurysm repair of a mean size of 64 ± 13 mm using a custom made device. Primary technical success was achieved in 28 of 30 patients (93%) and assisted primary technical success in 29 of 30 patients (97%). The two technical failures included open conversion to repair a ruptured iliac artery and restenting of a dissected superior mesenteric artery which was recognized hours after the index procedure had finished. The mean procedure time was 277 ± 153 minutes, fluoroscopy time 79 ± 36 minutes, dose area product 112 ± 90 Gy cm2, and contrast volume 87 ± 46 mL. All renal fenestrations were successfully stented without type III endoleak on completion angiogram; the preloaded guiding sheaths were used for 53 of 58 renal arteries (91%). Challenges related to learning to the use of the modified preloaded system were experienced early and had no clinical consequences. Major complications occurred in seven cases (23%), including two perioperative deaths because of stroke and sepsis following primary conversion attributable to iliac rupture. There were no target vessel occlusions or type I/III endoleaks found on postoperative imaging.Conclusions
Based on early experience, the modified preloaded system can be safely and effectively used during FEVAR, with good technical result and a short period of learning. This device expands treatment to patients with compromised iliac access, thus, additional patients and more follow-up will be required to determine unique risks of operating in this patient population. 相似文献20.
Hochedez P Rosine J Théodose R Abel S Bourhy P Picardeau M Quénel P Cabié A 《The American journal of tropical medicine and hygiene》2011,84(4):621-626
Three athletes who participated in a race in the tropical forest of the Caribbean island of Martinique were subsequently diagnosed with leptospirosis using polymerase chain reaction (PCR). We investigated an outbreak to evaluate possible risk factors, and to determine the appropriate public health recommendations. Of 230 athletes, we contacted 148 (64%) and 20 (13.5%) met our case definition. Five were hospitalized and none were fatal. Ten (91%) of the 11 ill athletes who were tested were confirmed by PCR or serology. Serogroup Pyrogenes was commonly found. Cutaneous cuts, reported by 14 (73.7%), was the only potential risk factor using univariate analysis. Sporting event participants in tropical areas should be made aware of specific warnings and recommendations concerning the risk of leptospirosis, especially after periods of heavy rainfall or flooding. Rapid diagnostic assays such as PCR are particularly appropriate in this setting for early diagnosis and for formulating public health recommendations. 相似文献