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ZHENPING TIAN CHING GU ROGER W. ROESKE MINGMING ZHOU ROBERT L. VAN ETTEN 《Chemical biology & drug design》1993,42(2):155-158
A practical and convenient procedure for making phosphotyrosine-containing peptides by the solid-phase method was developed. Phosphotyrosine was incorporated via Boc-Tyr(PO3Bzl2)-OH. The completed peptide was cleaved from the solid support by treatment with 1 M TMSBr-thioanisole-TFA. By gel-phase 31P-NMR spectroscopy we found that one of the benzyl protecting groups on phosphate was completely removed by two consecutive runs of Boc deprotection with 50% TFA-DCM. However, the other benzyl group remained intact throughout the synthesis (35 cycles). 相似文献
94.
ROBERT W. SHAW HAMISH M. ERASER 《BJOG : an international journal of obstetrics and gynaecology》1984,91(9):913-916
Summary. A luteinizing hormone releasing hormone agonist, d-Ser(But)6 des Gly10 -LHRH ethylamide, was administered intranasally to four women with unexplained ovulatory menorrhagia. Dramatic reductions in excessive menstrual blood losses were induced without significant side-effects, although the pattern of loss returned to pretreatment levels within 2 months of ceasing therapy. 相似文献
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Modification of the kaolin clotting time has produced a reliable, simple assaysystem for antihemophilic factor. This system has been utilized for an investigation of the nature and mode of action of the circulating anticoagulantdirected against antihemophilic factor.The anticoagulant has been shown to be present in equal amounts in plasmaand serum, to be associated with fractions II and III of plasma prepared bythe method of Cohn, to be relatively heat stable and to be stable for prolonged periods at -20 C.Investigation of the kinetics of the anticoagulant-antihemophilic reactionhas demonstrated its temperature, time, pH, and substrate concentration dependency. The anticoagulant is not inactivated during the reaction with antihemophilic factor, and there is a proportional relationship between the anticoagulant and the antihemophilic factor during their interaction.No antigen-antibody manifestations could be detected during the anticoagulant-antihemophilic factor reaction. These characteristics support thehypothesis that the inactivation of antihemophilic factor by specific circulatinganticoagulants is enzymatic. Submitted on February 19, 1962 Accepted on April 6, 1962 相似文献
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HERSCHEL S. ZACKHEIM ROBERT J. FELDMANN CHAPMAN LINDSAY HOWARD I. MAIBACH 《The British journal of dermatology》1977,97(1):65-67
The total percutaneous absorption of I,3-bis(2-chloroethyl)-i-nitrosourea (BCNU, carmustine) in 5 patients with mycosis fungoides, based on the 5 day urinary excretion of 14C-labelled BCNU, ranged from 5 to 28%. Absorption through diseased skin was greater than through uninvolved skin. 相似文献
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