Loss of ATE1-mediated arginylation leads to impaired platelet myosin phosphorylation,clot retraction,and in vivo thrombosis formation

Protein arginylation by arginyl–transfer RNA protein transferase (ATE1) is emerging as a regulator protein function that is reminiscent of phosphorylation. For example, arginylation of β-actin has been found to regulate lamellipodial formation at the leading edge in fibroblasts. This finding suggests that similar functions of β-actin in other cell types may also require arginylation. Here, we have tested the hypothesis that ATE1 regulates the cytoskeletal dynamics essential for in vivo platelet adhesion and thrombus formation. To test this hypothesis, we generated conditional knockout mice specifically lacking ATE1 in their platelets and in their megakaryocytes and analyzed the role of arginylation during platelet activation. Surprisingly, rather than finding an impairment of the actin cytoskeleton structure and its rearrangement during platelet activation, we observed that the platelet-specific ATE1 knockout led to enhanced clot retraction and in vivo thrombus formation. This effect might be regulated by myosin II contractility since it was accompanied by enhanced phosphorylation of the myosin regulatory light chain on Ser19, which is an event that activates myosin in vivo. Furthermore, ATE1 and myosin co-immunoprecipitate from platelet lysates. This finding suggests that these proteins directly interact within platelets. These results provide the first evidence that arginylation is involved in phosphorylation-dependent protein regulation, and that arginylation affects myosin function in platelets during clot retraction.     

中医药防治肾间质纤维化的研究概况

通过分析近年来的相关文献,对肾间质纤维化的中医病因病机及中医药防治现状进行梳理,以期对临床辨治本病提供参考。     

老年胆囊炎胆石症手术治疗效果观察

目的:探讨老年胆囊炎胆石症手术治疗。方法:选择100例老年胆囊炎及胆石症患者,回顾手术治疗资料。结果:100例患者中,治愈94例,死亡6例。结论:充分做好手术后期处理工作,就能够防止术后并发症,降低患者死亡率。     

高等医学院校公共英语对医学专业英语教学的充实作用分析

本文通过对医学专业英语教学中普遍存在的主要问题与公共英语充实医学专业英语教学的必要性分析,进一步阐述了公共英语充实医学专业英语教学的途径,为探讨医学专业英语教学改革的发展拓展思路。     

温阳补肾法治疗骨质疏松性骨折

目的 探讨温阳补肾法治疗骨质疏松性骨折对患者疼痛介质及血清sVCAM-1、PDGF、BGP水平的影响.方法 选择骨质疏松性骨折患者106例作为研究对象,通过随机数字表法分为研究组与对照组,各53例.对照组采用常规保守治疗,研究组在对照组的基础上予温阳补肾法进行治疗,2组均治疗3个月.比较2组治疗3个月后的临床疗效;对比2组治疗前、治疗3个月后的骨痂生长评分、中医证候积分、血清疼痛介质及骨代谢指标水平.结果 治疗3个月后,研究组总有效率(96.23％,51/53)高于对照组(75.47％,40/53)(P<0.05).治疗3个月后,2组骨痂生长评分、中医证候积分、血清PGE2、5-HT、NO、sVCAM-1水平较治疗前降低,研究组低于对照组(P<0.05);2组血清PDGF、BGP水平较治疗前升高,研究组高于对照组(P<0.05).结论 温阳补肾法治疗骨质疏松性骨折,可明显降低患者血清疼痛介质水平,减轻疼痛,并能降低血清sVCAM-1水平,升高血清PDGF、BGP水平,促进骨折愈合,有助于提高临床疗效.     

Correlation of Echocardiographic Epicardial Fat Thickness with Severity of Coronary Artery Disease in Patients with Acute Myocardial Infarction

The aim of this study was to test the hypotheses that epicardial adipose tissue (EAT) can be a marker of severe coronary artery disease in patients with acute myocardial infarction. Overall, 373 cases who underwent coronary angiography were classified into 2 groups by SYNTAX score: low‐score and high‐score group. EAT was measured by transthoracic echocardiography. Obtained data were compared using Pearson correlation analyses and univariate and multiple logistic regression analysis. The results showed that EAT in the high‐score group was significantly greater than in the normal group (5.6 ± 1.1 vs. 4.1 ± 1.0 mm, P < 0.01). EAT had a positive correlation with SYNTAX score (r = 0.61, P < 0.01). Receiver operating characteristic (ROC) curve analyses showed that EAT could reliably discriminate patients with high SYNTAX score (≥33) [AUC: 0.86, 95% confidence interval (CI): 0.822–0.898, P < 0.01]. Multivariate regression analyses showed that EAT was an independent predictor for major in‐hospital events. These data showed an association between EAT and SYNTAX score.     

烟台15种海产品中锑含量分析

目的测定烟台市15种海产品中锑的含量,评价污染状况,探讨每人每日摄入限量。方法采用氢化物原子荧光光谱法测定烟台市15种海产品中锑的含量。结果所检测海产品中锑平均含量为6.3μg/kg,各种海产品的锑含量均低于香港公众健康与市政服务条例评价标准,且总体分布远离标准限值。单因子污染指数评价结果显示,各种海产品中锑含量均处于正常水平。蛏子中的平均含量高于其他海产品(F=118.497,P<0.05)。蛏子的每人每日允许摄入量男性、女性分别为1.81和1.61kg。结论烟台市15种海产品中均检出锑,但均未受到污染。海产品的允许摄入量较高,食用安全风险较低。