阿托伐他汀在缺血性卒中患者中的疗效研究

目的探讨阿托伐他汀对缺血性卒中患者的疗效。方法收集2012年6月~2014年2月来我院诊治的缺血性卒中患者311例为研究对象,依据其来诊的顺序随机分为实验组与对照组。两组均给予常规内科治疗,实验组加用阿托伐他汀治疗,治疗3个月后,观察两组的治疗效果。结果实验组的临床疗效明显高于对照组;治疗后实验组的总胆固醇、甘油三酯及低密度脂蛋白明显低于对照组,而高密度脂蛋白明显高于对照组。结论阿托伐他汀可以改善缺血性卒中患者血脂水平,提高缺血性卒中患者的治疗效果。     

~(99)Tc~m-MIBI显像在非霍奇金淋巴瘤化疗疗效评价中的价值

目的探讨99Tcm-MIBI显像在非霍奇金淋巴瘤疗效评价及提示预后方面的临床价值。方法对20例非霍奇金淋巴瘤病人于化疗前后分别行99Tcm-MIBI早期(10 min)与延迟(120 min)双时相显像,计算每例病人早期摄取率(EUR)、延迟期摄取率(DUR)及洗脱率(WR%)并进行比较。对99Tcm-MIBI显像结果进行疗效评价。平均随访时间34个月。结果 8例病人化疗后99Tcm-MIBI显像阴性,达完全缓解(CR)。12例病人化疗后99Tcm-MIBI显像阳性,其中4例达部分缓解(PR),2例处于稳定(SD)状态,6例进展(PD)。将病人分为化疗有效组(CR+PR)12例,疗效不佳组(SD+PD)8例。20例病人化疗后DUR明显低于化疗前(1.2±0.7∶2.9±1.2,P0.05)。化疗有效组与疗效不佳组相比,前者化疗前的DUR值高于后者(2.9±1.2∶1.5±0.9,P0.05),WR%低于后者(21.9±2.4∶42.7±5.6,P0.05),而前者化疗后的DUR值低于后者(1.0±0.2∶2.0±0.3,P0.05)。结论 99Tcm-MIBI作为一种功能性显像,有助于预测、评价非霍奇金淋巴瘤病人的疗效及预后。     

Age-related macular degeneration in China: a population-based and clinical study

Background

Age-related macular degeneration (AMD) is one of the most common causes of irreversible blindness worldwide. We assessed its epidemiology and related factors in China.

维生素D对胰岛β细胞功能及胰岛素敏感性的影响

维生素D在维持正常胰岛β细胞功能方面发挥举足轻重的作用。婴幼儿时期维生素D的缺乏可使1型糖尿病的发病率增高,并造成β细胞合成和分泌胰岛素的功能减退。而维生素D的干预则可以延缓和改善这一过程,这可能源于维生素受体(VDR)介导的免疫调节作用。大量研究也揭示了维生素D不足和2型糖尿病之间的内在关联,其对胰岛β细胞功能和胰岛素的敏感性的影响也得到证实,这些效应可能是通过对VDR基因的作用以及对钙磷平衡的影响而介导的,转录因子过氧化酶增殖物活化受体(PPAR)-δ在其中也扮演一定角色。合理使用维生素D有望成为糖尿病预防与治疗的重要手段。     

带蒂胸锁乳突肌锁骨骨膜皮瓣修复喉气管缺损的初步研究

目的:探讨带蒂胸锁乳突肌锁骨骨膜皮瓣修复肿瘤切除术后喉气管缺损的可行性。方法:回顾2007-2013年6例因甲状腺乳头状癌(4例)及声门下喉鳞状细胞癌(6例)(T1~2N1~2M0,UICC,2002)手术切除致喉气管部分缺损病例,以带蒂胸锁乳突肌锁骨骨膜皮瓣修复喉气管缺损区,术腔置喉模支撑成形,术后半年,如局部无复发,气管套管堵管无困难,则拔管封闭气管造瘘口。随访半年~3年。结果:6例患者中4例顺利拔管,1例间断堵管,1例保留气管套管呼吸。结论:选择适当病例,用带蒂胸锁乳突肌锁骨骨膜皮瓣可以修复喉气管部分缺损,在发音和呼吸方面达到满意的临床效果。     

Single-molecule force measurement via optical tweezers reveals different kinetic features of two BRaf mutants responsible for cardio-facial-cutaneous (CFC) syndrome: errata

We correct the omission of the construct and protein purification method in our recent paper [Biomed. Opt. Express 4(12), 2835–2845 (2013)].OCIS codes: (170.0170) Medical optics and biotechnology, (170.1420) Biology, (170.4520) Optical confinement and manipulation, (350.4855) Optical tweezers or optical manipulationWe add the construct and protein purification method mistakenly omitted in our recent paper [1]. The constructs were made following our previous studies [2, 3]. In brief, GST tagged Ras binding domain (RBD) and cysteine rich domain (CRD) expression constructs were generated by subcloning the two domains (amino acids 136-298) of wildtype or mutant human BRaf into pGEX4T-1 (GST-fusion vector, GE Healthcare). GST-RBD-CRD fusion proteins were induced in XL10-Gold E. coli strain (#200314, Stratagene), purified using Glutathione Sepharose 4B (#17-0756-01, GE Healthcare), and concentrated with Amicon Ultra centrifugal filters (Ultracel-30K, Millipore). Hexahistidine (His)-tagged Ras(WT) expression constructs were made by subcloning PCR-amplified full-length human H-Ras into pET28a( + ) (His-tag vector, Novagen). His-tagged Ras(WT) proteins were induced in BL21-CodonPlus (DE3)-RILP strain (#230280, Agilent Technologies), purified using Ni-NTA His-Bind Purification Kit (#70751-3, EMD Millipore Novagen), and quantified by Bradford assay kit (Bio-Rad).