全文获取类型
收费全文 | 2969篇 |
免费 | 181篇 |
国内免费 | 8篇 |
专业分类
耳鼻咽喉 | 31篇 |
儿科学 | 210篇 |
妇产科学 | 52篇 |
基础医学 | 288篇 |
口腔科学 | 64篇 |
临床医学 | 219篇 |
内科学 | 606篇 |
皮肤病学 | 129篇 |
神经病学 | 243篇 |
特种医学 | 37篇 |
外科学 | 418篇 |
综合类 | 38篇 |
一般理论 | 4篇 |
预防医学 | 122篇 |
眼科学 | 139篇 |
药学 | 319篇 |
中国医学 | 15篇 |
肿瘤学 | 224篇 |
出版年
2023年 | 13篇 |
2022年 | 44篇 |
2021年 | 95篇 |
2020年 | 61篇 |
2019年 | 84篇 |
2018年 | 118篇 |
2017年 | 88篇 |
2016年 | 95篇 |
2015年 | 87篇 |
2014年 | 136篇 |
2013年 | 163篇 |
2012年 | 234篇 |
2011年 | 228篇 |
2010年 | 165篇 |
2009年 | 109篇 |
2008年 | 208篇 |
2007年 | 178篇 |
2006年 | 154篇 |
2005年 | 170篇 |
2004年 | 181篇 |
2003年 | 123篇 |
2002年 | 109篇 |
2001年 | 34篇 |
2000年 | 28篇 |
1999年 | 23篇 |
1998年 | 36篇 |
1997年 | 19篇 |
1996年 | 24篇 |
1995年 | 22篇 |
1994年 | 12篇 |
1993年 | 8篇 |
1992年 | 11篇 |
1991年 | 20篇 |
1990年 | 8篇 |
1989年 | 9篇 |
1988年 | 6篇 |
1987年 | 6篇 |
1986年 | 3篇 |
1984年 | 3篇 |
1983年 | 6篇 |
1982年 | 4篇 |
1981年 | 7篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1974年 | 2篇 |
1972年 | 1篇 |
1968年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有3158条查询结果,搜索用时 15 毫秒
11.
12.
13.
Pharmaceutical Research - 相似文献
14.
15.
Van Buskirk Glenn A. González Mario A. Shah Vinod P. Barnhardt Scott Barrett Colin Berge Stephen Cleary Gary Chan Keith Flynn Gordon Foster Thomas Gale Robert Garrison Raymond Gochnour Scott Gotto Amanda Govil Sharad Gray Vivian A. Hammar James Harder Samuel Hoiberg Charles Hussain Ajaz Karp Carol Llanos Hector Mantelle Juan Noonan Patrick Swanson David Zerbe Horst 《Pharmaceutical research》1997,14(7):848-852
Pharmaceutical Research - 相似文献
16.
Recruitment of complement factor H-like protein 1 promotes intracellular invasion by group A streptococci 总被引:9,自引:0,他引:9 下载免费PDF全文
Numerous microbial pathogens exploit complement regulatory proteins such as factor H (FH) and factor H-like protein 1 (FHL-1) for immune evasion. Fba is an FHL-1 and FH binding protein expressed on the surface of the human pathogenic bacterium, Streptococcus pyogenes, a common agent of pharyngeal, skin, and soft-tissue infections. In the present study, we demonstrate that Fba and FHL-1 work in concert to promote invasion of epithelial cells by S. pyogenes. Fba fragments were expressed as recombinant proteins and assayed for binding of FHL-1 and FH by Western blotting, enzyme-linked immunosorbent assay, and surface plasmon resonance. A binding site for FHL-1 and FH was localized to the N-terminal half of Fba, a region predicted to contain a coiled-coil domain. Deletion of this coiled-coil domain greatly reduced FHL-1 and FH binding. PepSpot analyses identified a 16-amino-acid segment of Fba which overlaps the coiled-coil domain that binds both FHL-1 and FH. To localize the Fba binding site in FHL-1 and FH, surface plasmon resonance was used to assess the interactions between the streptococcal protein and a series of recombinant FH deletion constructs. The Fba binding site was localized to short consensus repeat 7 (SCR 7), a domain common to FHL-1 and FH. SCR 7 contains a heparin binding site, and heparin was found to inhibit FHL-1 binding to Fba. FHL-1 promoted entry of Fba(+) group A streptococci into epithelial cells in a dose-dependent manner but did not affect invasion by an isogenic fba mutant. To our knowledge, this is the first report of a bacterial pathogen exploiting a soluble complement regulatory protein for entry into host cells. 相似文献
17.
H H Goebel W Zeman V K Patel R K Pullarkat H G Lenard 《Mechanisms of ageing and development》1979,10(1-2):53-70
In 4 patients with neuronal ceroid-lipofuscinoses (NCL) (3 patients with the junvenile type, 1 patient with the late infantile type), the ultrastructural spectrum of residual bodies in the central and peripheral nervous system presented curvilinear profiles in all cases and regions investigated and many more ultrastructural patterns within and beyond regions commonly accessible to biopsy, probably due to age dependence, local tissue and cellular biochemical factors. Sampling from basal ganglia especially yielded combined curvilinear-fingerpint bodies, from peripheral ganglia additional membranous bodies. Residual bodies in NCL were present in almost every cell type, similar to the distribution of regular lipofuscin. Although the classical subgroups of NCL contain electronmicroscopically well defined residual bodies, permitting distinction of the late infantile type from the juvenile type, the ultrastructural differences are more of a quantitative than of a qualitative nature. However, they are not pathognomonic. N.m.r. spectra of ceroid and lipofuscin support the concept of their biochemical similarity, and argue against the proposition that they contain a single major component. 相似文献
18.
Vinod Chandran 《Expert Review of Clinical Immunology》2020,16(5):471-478
ABSTRACT
Introduction
Biomarkers may help influence long-term outcomes of psoriatic disease by improving the objective assessment of the presence and severity of psoriatic arthritis (PsA) and by guiding treatment selection. However, there are no validated biomarkers for PsA. 相似文献19.
Outcomes of unrelated umbilical cord blood transplantation for X-linked adrenoleukodystrophy. 总被引:1,自引:0,他引:1
Donald Beam Michele D Poe James M Provenzale Paul Szabolcs Paul L Martin Vinod Prasad Suhag Parikh Tim Driscoll Srini Mukundan Joanne Kurtzberg Maria L Escolar 《Biology of blood and marrow transplantation》2007,13(6):665-674
Adrenoleukodystrophy (ALD) is an X-linked disorder caused by a defect in the metabolism of long chain fatty acids leading to demyelination, neurodegeneration, and death. The disease typically presents in young boys and adolescent boys. Allogeneic bone marrow transplantation has been used to halt progression of the disease. However, many patients lack suitable HLA- matched related donors and must rely on unmatched donors for a source of stem cells. The purpose of this study was to evaluate outcomes of unrelated donor umbilical cord blood transplantation after chemotherapy-based myeloablative conditioning and retrospectively determine if baseline studies correlate and help predict outcome. Between November 22, 1996, and November 3, 2005, 12 boys with X-linked ALD who lacked HL- matched related donors were referred to Duke University Medical Center for transplantation. These children were conditioned with myeloablative therapy including busulfan, cyclophosphamide, and antithymocyte globulin before receiving umbilical cord-blood transplants from unrelated donors. Baseline studies of neurophysiologic, neuroimaging, and neurodevelopmental status were performed and patients were subsequently evaluated for survival, engraftment, graft-versus-host disease, and neurodevelopmental outcomes. A substudy evaluated whether baseline neuroimaging and neurophysiologic studies correlated with cognitive and motor function and if these studies were predictive of posttransplantation outcomes. The umbilical cord blood grafts had normal levels of very long chain fatty acids. They delivered a median of 6.98 x 10(7) nucleated cells per kilogram of recipient body weight and were discordant for up to 4 of 6 HLA markers. Neutrophil engraftment occurred at a median of 22.9 days after transplantation. Three patients had grade II-IV acute graft-versus-host disease; 2 had extensive chronic graft-versus-host disease. Cumulative incidence of overall survival of the group at 6 months is 66.7% (95% confidence interval 39.9-93.3%). Median follow-up was 3.3 years (range 12 days to 6.3 years). As previously reported with bone marrow transplantation, symptomatic patients faired poorly with lower survival and rapid deterioration of neurologic function. This study included 3 patients transplanted at a very young age (2.6-3.5 years) before the onset of clinical symptoms who continue to develop at a normal rate for 3-5 years posttransplant. Although baseline Loes scores correlated with cognitive and motor outcome, neurophysiologic studies failed to show statistically significant differences. Transplantation of boys with X-linked ALD using partial HLA-matched umbilical cord blood yields similar results to those previously reported after bone marrow transplantation. Superior outcomes were seen in neurologically asymptomatic boys less than 3.5 years of age at the time of transplantation. Baseline Loes scores were a strong predictor of cognitive and motor outcome. 相似文献
20.
Isolated primary amyloid tumor of bone is rare; however, preoperative diagnosis can be rewarding because the prognosis is excellent. There are no clinical or roentgenographic criteria that can establish this diagnosis. There are few previous reports of fine-needle aspiration (FNA) where diagnosis of amyloidoma was made retrospectively. They describe the presence of extracellular hyaline material along with plasma cells and lymphocytes. A 35-year-old female was referred to the FNA clinic with swelling in the right upper chest wall. Radiologic findings revealed a destructive lytic lesion involving the bodies of D1 and D2 vertebrae with extension into surrounding soft tissue. Repeated FNA smears were hypocellular but had abundant homogeneous flocculent material, which stained positive with Congo red. A few plasma cells and foreign-body giant cells were also seen. We conclude that preoperative FNA cytology diagnosis of amyloid tumor is possible. Hypocellular smears with flocculent material, plasma cells, and foreign-body giant cells in absence of granulation tissue should suggest the diagnosis. 相似文献