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排序方式: 共有359条查询结果,搜索用时 15 毫秒
81.
Autologous peripheral hematopoietic stem cell transplantation restores hematopoietic function following marrow ablative therapy 总被引:2,自引:0,他引:2
From ten patients with advanced malignant disease involving the bone marrow, autologous hematopoietic stem cells were collected from the peripheral blood during eight four-hour pheresis procedures and cryopreserved. No manipulations to increase the number of stem cells circulating in the blood were used during the collections. Following marrow ablative chemotherapy or chemoradiotherapy, the autologous cells were thawed and infused intravenously (IV). WBCs reappeared in the circulation at a median of eight days (range seven to 11 days) after stem cell infusion. Two patients died early, whereas the other eight reached normal numbers of circulating granulocytes that have persisted for up to greater than 20 months. These eight patients became independent of RBC transfusions (hemoglobin concentration greater than 10 g/dL) at a median of 27 days (range 11 to 58 days) after transplantation. One patient received platelet transfusions for counts less than 50 x 109)/L, one patient developed a clinical picture of idiopathic thrombocytopenic purpura, and six patients maintained a platelet count greater than 20 x 10(9)/L at a median of 23 days (range 14 to 25 days) following stem cell infusion. This technique allows patients ineligible for autologous bone marrow transplantation due to unacceptable anesthetic risks, prior pelvic irradiation, or bone marrow metastases to receive marrow ablative therapy. 相似文献
82.
Blood cell dynamics in P-selectin-deficient mice 总被引:9,自引:4,他引:9
Johnson RC; Mayadas TN; Frenette PS; Mebius RE; Subramaniam M; Lacasce A; Hynes RO; Wagner DD 《Blood》1995,86(3):1106-1114
P-selectin is expressed on the surfaces of activated platelets and endothelium where it mediates binding to leukocytes. P-selectin- deficient mice were shown to exhibit peripheral neutrophilia (Mayadas et al: Cell 74:541, 1993). We now show that this is not caused by changes in bone marrow precursors nor by a lack of neutrophil margination. Both P-selectin-positive and -negative animals displayed similar increases in peripheral blood neutrophil numbers after injection of epinephrine. However, clearance of 51Chromium-labeled neutrophils is delayed in mice deficient for P-selectin, indicating that the neutrophilia is at least in part the result of delayed removal. We detected no obvious alterations in lymphocyte differentiation, distribution, or adhesion to high endothelial venules in peripheral lymph nodes. Through intravital microscopy, we examined the impact of P-selectin deficiency on leukocyte/endothelial interaction beyond the initial stages of inflammation. Four hours after the administration of an inflammatory irritant, leukocyte rolling was observed even in the absence of P-selectin. There were significantly fewer rolling cells relative to wild-type mice, and their velocity was reduced. Moreover, in the peritonitis model, the number of peritoneal macrophages in wild-type mice increased threefold at 48 hours, whereas the macrophages in the mutant mice remained near baseline levels. Thus, whereas P-selectin is known to be involved in early stages of an inflammatory response, our results indicate that it is additionally responsible for leukocyte rolling and macrophage recruitment in more prolonged tissue injury. 相似文献
83.
Anti-My-28, an antigranulocyte mouse monoclonal antibody, binds to a sugar sequence in lacto-N-neotetraose 总被引:5,自引:0,他引:5
Spitalnik SL; Schwartz JF; Magnani JL; Roberts DD; Spitalnik PF; Civin CI; Ginsburg V 《Blood》1985,66(2):319-326
Anti-My-28 is an IgM kappa monoclonal antibody produced by a hybridoma prepared from spleen cells of a mouse immunized with normal human granulocytes. By immunofluorescence it binds to human granulocytes but not to monocytes and lymphocytes. However, after treating cells with neuraminidase, the antibody also binds to lymphocytes and monocytes and to many leukemic cell lines and patient leukemic blast cells. Anti-My- 28 binds to several neutral glycolipids and desialylated gangliosides of leukocytes and erythrocytes as detected by radioimmunoassay and immunostaining of thin-layer chromatograms. It recognizes a sugar sequence in lacto-N-neotetraose, Gal beta 1-4GlcNAc beta 1-3Gal beta 1- 4Glc. This tetrasaccharide occurs in the glycolipids paragloboside and sialosylparagloboside, and its distal trisaccharide sequence is found in higher glycolipids and in glycoproteins. 相似文献
84.
Carlos Antonio Negrato Renan M Montenegro Jr Rosiane Mattar Lenita Zajdenverg Rossana PV Francisco Belmiro Gonçalves Pereira Mauro Sancovski Maria Regina Torloni Sergio A Dib Celeste E Viggiano Airton Golbert Elaine CD Moisés Maria Isabel Favaro Iracema MP Calderon Sonia Fusaro Valeria DD Piliakas José Petronio L Dias Marilia B Gomes Lois Jovanovic 《Diabetology & metabolic syndrome》2010,2(1):1-14
There is an urgent need to find consensus on screening, diagnosing and treating all degrees of DYSGLYCEMIA that may occur during pregnancies in Brazil, considering that many cases of DYSGLYCEMIA in pregnant women are currently not diagnosed, leading to maternal and fetal complications. For this reason the Brazilian Diabetes Society (SBD) and the Brazilian Federation of Gynecology and Obstetrics Societies (FEBRASGO), got together to introduce this proposal. We present here a joint consensus regarding the standardization of clinical management for pregnant women with any degree of Dysglycemia, on the basis of current information, to improve medical assistance and to avoid related complications of Dysglycemia in pregnancy to the mother and the fetus. This consensus aims to standardize the diagnosis among general practitioners, endocrinologists and obstetricians allowing the dissemination of information in basic health units, public and private services, that are responsible for screening, diagnosing and treating disglycemic pregnant patients. 相似文献
85.
Löhning M Hegazy AN Pinschewer DD Busse D Lang KS Höfer T Radbruch A Zinkernagel RM Hengartner H 《The Journal of experimental medicine》2008,205(1):53-61
Many vaccination strategies and immune cell therapies aim at increasing the numbers of memory T cells reactive to protective antigens. However, the differentiation lineage and therefore the optimal generation conditions of CD4 memory cells remain controversial. Linear and divergent differentiation models have been proposed, suggesting CD4 memory T cell development from naive precursors either with or without an effector-stage intermediate, respectively. Here, we address this question by using newly available techniques for the identification and isolation of effector T cells secreting effector cytokines. In adoptive cell transfers into normal, nonlymphopenic mice, we show that long-lived virus-specific memory T cells can efficiently be generated from purified interferon gamma-secreting T helper (Th) type 1 and interleukin (IL)-4- or IL-10-secreting Th2 effectors primed in vitro or in vivo. Importantly, such effector-derived memory T cells were functional in viral challenge infections. They proliferated vigorously, rapidly modulated IL-7 receptor expression, exhibited partial stability and flexibility of their cytokine patterns, and exerted differential effects on virus-induced immunopathology. Thus, cytokine-secreting effectors can evade activation-induced cell death and develop into long-lived functional memory cells. These findings demonstrate the efficiency of linear memory T cell differentiation and encourage the design of vaccines and immune cell therapies based on differentiated effector T cells. 相似文献
86.
87.
88.
Verghis SB; Essigmann JM; Kadlubar FF; Morningstar ML; Lasko DD 《Carcinogenesis》1997,18(12):2403-2414
Mutagenesis by the human bladder carcinogen 4-aminobiphenyl (ABP) was
studied in single-stranded DNA from a bacteriophage M13 cloning vector. In
comparison to ABP lesions in double-stranded DNA, lesions in single-
stranded DNA were approximately 70-fold more mutagenic and 50-fold more
genotoxic. Sequencing analysis of ABP-induced mutations in the lacZ gene
revealed exclusively base-pair substitutions, with over 80% of the
mutations occurring at G sites; the G at position 6310 accounted for 25% of
the observed mutations. Among the sequence changes at G sites, G- ->T
transversions predominated, followed by G-->C transversions and G--
>A transitions. In order to further elucidate the mutagenic mechanism of
ABP, an oligonucleotide containing the major DNA adduct, N-
(deoxyguanosin-8-yl)-4-aminobiphenyl (dG(8-ABP)), was situated within the
PstI site of a single-stranded M13 genome. After in vivo replication of the
adduct containing ABP-modified and control (unadducted) genomes, the
mutational frequency and mutational specificity of the dG(8-ABP) lesion
were determined. The targeted mutational efficiency was approximately
0.01%, and the primary mutation observed was the G-->C transversion.
Thus dG(8-ABP), albeit weakly mutagenic at the PstI site, can contribute to
the mutational spectrum of ABP lesions.
相似文献
89.
90.
Stark DD; Moseley ME; Bacon BR; Moss AA; Goldberg HI; Bass NM; James TL 《Radiology》1985,154(1):137-142
Experimental animals that had been given excess iron in their diet were studied by magnetic resonance (MR) imaging in vivo and by magnetic resonance (MR) spectroscopy in vitro. Hepatic iron overload in patients with transfusional iron excess was studied by MR imaging, and isolated iron protein fractions were studied in vitro by MR spectroscopy. The spin echo image intensity of livers with iron overload was decreased because of the extreme decreases in T2 compared with normal; T1 was decreased only moderately. The relaxation rates 1/T2 and 1/T1 both showed a linear relationship to hepatic iron levels. Ferritin solutions showed moderate decreases in T2 and mild decreases in T1. The T2 relaxivity of ferritin, which is due to the iron core rather than the apoferritin protein shell, does not appear sufficient to account for the extreme decrease in T2 observed in hepatic iron overload. Low molecular weight cytosol iron is present in lower concentrations than ferritin but potentially has much greater relaxivity and may contribute to the MR findings. These techniques may be useful in other studies of iron metabolism. 相似文献