Use of log-scaled crowded visual acuity charts in clinical studies regarding amblyopia

Graefe's Archive for Clinical and Experimental Ophthalmology - Log-scaled crowded charts using standardized testing protocol are essential for precise and reproducible visual acuity (VA)...     

The effectiveness of auxiliary features on a tooth preparation with inadequate resistance form

STATEMENT OF PROBLEM: No study has evaluated the efficacy of auxiliary tooth preparation elements for crowns with originally reduced resistance form. PURPOSE: This study evaluated the effects of different auxiliary preparation features on the resistance form of crowns with reduced axial wall and total occlusal convergence. MATERIAL AND METHODS: An Ivorine tooth was prepared on a milling machine with 20-degree total occlusal convergence (TOC), 2.5 mm of occlusocervical dimension, and a shoulder finish line. This design lacked geometric resistance form. The crown preparation was subsequently modified to include mesiodistal grooves, mesiodistal boxes, buccolingual grooves, occlusal inclined planes, an occlusal isthmus, and reduced TOC in the axial wall from 20 to 8 degrees TOC in the cervical 1.5 mm of the axial wall. The grooves and boxes were placed into the tooth with the same 20-degree TOC as the initial axial walls. Ten standardized metal dies were used for each preparation design. Standardized complete metal crowns were fabricated for all specimens. The metal crowns were cemented on metal dies with resin-modified glass ionomer cement. A strain gauge was placed at the mid-lingual cervical area of each crown preparation margin. The resistance of each specimen was evaluated when force was applied at a 45-degree angulation to the long axis of the die in a lingual to buccal direction. The peak loads during crown dislodgment, as well as the tensile stress at the mid-lingual cervical area, were measured using a universal testing machine (Kgs) for each specimen. The control group consisted of 10 dies, with the original crown preparation having no geometric resistance form and no auxiliary preparation features. Strain gauges provided the force (Kgs) that resulted in electric currency disrupt at the crown/die interface, thus providing data regarding the force required for slight crown micromovement (2 microm). Data between control and experimental groups were compared using the Mann-Whitney U test (alpha=.05). RESULTS: Proximal grooves, proximal boxes, buccolingual grooves, occlusal inclined planes, and occlusal isthmuses were not effective at increasing a crown's resistance to dislodgement when the tooth preparation lacked resistance. The only crown modification that offered enhanced resistance form when compared with the control group was the reduced TOC in the cervical half of the axial wall. CONCLUSION: Within the limitations of this in vitro study the crown preparation modification that significantly enhanced the resistance form of a compromised tooth preparation was reducing the TOC at the cervical aspect of the axial wall. Placing auxiliary retentive features such as grooves and boxes into a compromised tooth preparation (2.5 mm occlusocervical dimension and 20-degree TOC) was not effective when these retentive features possessed the same 20-degree TOC as the prepared axial walls.     

In vitro elution of moxifloxacin and fusidic acid by a synthetic crystallic semihydrate form of calcium sulphate (Stimulan)

Stimulan was evaluated in vitro as a biodegradable carrier for local delivery of moxifloxacin and fusidic acid. Moxifloxacin or fusidic acid was mixed with calcium sulphate at a ratio of 95:5 to prepare five replicas per antibiotic. In vitro elution was estimated daily using a high-performance liquid chromatography (HPLC) system. Elution of moxifloxacin lasted for 31 days. Eluted concentrations reached their peak on Day 13 (mean level 745 microg/mL); the lowest eluted concentration was detected on Day 30 (mean level 367 microg/mL). Elution of fusidic acid lasted for 14 days. Eluted concentrations reached their peak on Day 6 (mean value 249.5 microg/mL); the lowest eluted concentration was detected on Day 13 (mean value 10.9 microg/mL). The presented results revealed that Stimulan may allow adequate in vitro elution of moxifloxacin and fusidic acid. The latter results support the application of this system in experimental models of osteomyelitis.     

Biallelic loss of function variants in PPP1R21 cause a neurodevelopmental syndrome with impaired endocytic function

Next‐generation sequencing (NGS) has been instrumental in solving the genetic basis of rare inherited diseases, especially neurodevelopmental syndromes. However, functional workup is essential for precise phenotype definition and to understand the underlying disease mechanisms. Using whole exome (WES) and whole genome sequencing (WGS) in four independent families with hypotonia, neurodevelopmental delay, facial dysmorphism, loss of white matter, and thinning of the corpus callosum, we identified four previously unreported homozygous truncating PPP1R21 alleles: c.347delT p.(Ile116Lysfs*25), c.2170_2171insGGTA p.(Ile724Argfs*8), c.1607dupT p.(Leu536Phefs*7), c.2063delA p.(Lys688Serfs*26) and found that PPP1R21 was absent in fibroblasts of an affected individual, supporting the allele's loss of function effect. PPP1R21 function had not been studied except that a large scale affinity proteomics approach suggested an interaction with PIBF1 defective in Joubert syndrome. Our co‐immunoprecipitation studies did not confirm this but in contrast defined the localization of PPP1R21 to the early endosome. Consistent with the subcellular expression pattern and the clinical phenotype exhibiting features of storage diseases, we found patient fibroblasts exhibited a delay in clearance of transferrin‐488 while uptake was normal. In summary, we delineate a novel neurodevelopmental syndrome caused by biallelic PPP1R21 loss of function variants, and suggest a role of PPP1R21 within the endosomal sorting process or endosome maturation pathway.     

Carriage of Antibiotic-Resistant Streptococcus pneumoniae in Greek Infants and Toddlers

The prevalence, resistance patterns and serotypes of antibiotic-resistant Streptococcus pneumoniae strains recovered from Greek carriers under 24 months of age were studied. From February 1997 to April 1998, nasopharyngeal cultures were performed in 1269 children (ages 2–23 months, median 11 months) living in various areas of central and southern Greece. Resistance (including both intermediate and resistant isolates) to one or more antimicrobial agents was found in 132 of the 421 (31%) Streptococcus pneumoniae isolates, as follows: penicillin, 9% intermediate, 7.6% resistant; cefotaxime, 5.2% intermediate, 0.5% resistant; erythromycin, 0.7% intermediate, 18.1% resistant; clindamycin, 0.2% intermediate, 12.4% resistant; tetracycline, 0.7% intermediate, 16.4% resistant; chloramphenicol, 12.4% resistant; and trimethoprim-sulfamethoxazole, 3.8% intermediate, 14.3% resistant. The MICs of penicillin for 66% of the penicillin-nonsusceptible pneumococci were 1–4 μg/ml. Multidrug resistance was found in 64% of penicillin-nonsusceptible and 37% of penicillin-susceptible strains. Sixty-two percent of the penicillin-susceptible, multidrug-resistant strains belonged to serotype 6B and were resistant to all five non-β-lactam agents tested. This notable serotype 6B resistance pattern was described for the first time in a previous study performed from December 1995 to February 1996 in the city of Patras, southwestern Greece. Seventy-two percent of antibiotic-resistant isolates belonged to serotypes 6B, 9V, 14, 18C, 19F and 23F. These results document the spread of resistant pneumococcal strains in central and southern Greece, many of which are multidrug resistant.     

Transcatheter aortic valve replacement and stroke: a comprehensive review

Transcatheter aortic valve implantation (TAVR) has emerged as an alternative, rapidly evolving treatment option for patients with severe aortic stenosis and high surgical risk. Stroke is a devastating complication being confined mainly in the periprocedural and 30-day period following TAVR, with a lower and relatively constant frequency thereafter. Early stroke is mainly due to debris embolization during the procedure, whereas later events are associated with patient specific factors. Despite the fact that the rate of clinical stroke has been constantly decreasing compared to initial TAVR experience, modern neuro-imaging with MRI suggests that new ischemic lesions post-TAVR are almost universal. The impact of the latter is largely unknown. However, they seem to correlate with a reduction in neurocognitive function. Because TAVR is set to expand its indication to lower surgical-risk patients, stroke prophylaxis during and after TAVR becomes of paramount importance. Based on clinical and pathophysiological evidence, three lines of research are actively employed towards this direction: improvement in valve and delivery system technology with an aim to reduce manipulations and contact with the calcified aortic arch and native valve, antithrombotic therapy, and embolic protection devices. Careful patient selection, design of the procedure, and tailored antithrombotic strategies respecting the bleeding risks of this fragile population constitute the main defense against stroke following TAVR.     

Role of platelet-rich plasma in ischemic heart disease: An update on the latest evidence

Myocardial infarction is the most common cause of congestive heart failure. Novel strategies such as directly reprogramming cardiac fibroblasts into cardiomyocytes are an exciting area of investigation for repair of injured myocardial tissue. The ultimate goal is to rebuild functional myocardium by transplanting exogenous stem cells or by activating native stem cells to induce endogenous repair. Cell-based myocardial restoration, however, has not penetrated broad clinical practice yet. Platelet-rich plasma, an autologous fractionation of whole blood containing high concentrations of growth factors, has been shown to safely and effectively enhance healing and angiogenesis primarily by reparative cell signaling. In this review, we collected all recent advances in novel therapies as well as experimental evidence demonstrating the role of platelet-rich plasma in ischemic heart disease, focusing on aspects that might be important for future successful clinical application.