首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   114634篇
  免费   9978篇
  国内免费   6932篇
耳鼻咽喉   1215篇
儿科学   1628篇
妇产科学   806篇
基础医学   10642篇
口腔科学   1938篇
临床医学   12492篇
内科学   16391篇
皮肤病学   1429篇
神经病学   5606篇
特种医学   4861篇
外国民族医学   25篇
外科学   13189篇
综合类   22458篇
现状与发展   30篇
一般理论   10篇
预防医学   9843篇
眼科学   2014篇
药学   11656篇
  119篇
中国医学   6851篇
肿瘤学   8341篇
  2024年   547篇
  2023年   1270篇
  2022年   3227篇
  2021年   4609篇
  2020年   3527篇
  2019年   2889篇
  2018年   3008篇
  2017年   3273篇
  2016年   3206篇
  2015年   4907篇
  2014年   6264篇
  2013年   6662篇
  2012年   9868篇
  2011年   10509篇
  2010年   8272篇
  2009年   7097篇
  2008年   8109篇
  2007年   8082篇
  2006年   7213篇
  2005年   6461篇
  2004年   4779篇
  2003年   4488篇
  2002年   3830篇
  2001年   2376篇
  2000年   1791篇
  1999年   1128篇
  1998年   722篇
  1997年   733篇
  1996年   489篇
  1995年   445篇
  1994年   371篇
  1993年   238篇
  1992年   202篇
  1991年   174篇
  1990年   124篇
  1989年   100篇
  1988年   79篇
  1987年   73篇
  1986年   72篇
  1985年   41篇
  1984年   46篇
  1983年   27篇
  1982年   26篇
  1981年   19篇
  1980年   21篇
  1978年   19篇
  1975年   16篇
  1974年   12篇
  1973年   16篇
  1972年   18篇
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
62.
The involvement of the NMDA and non-NMDA receptors in the task-relatedneuronal activity of the primary motor cortex (MI), premotorcortex (PM), supplementary motor area (SMA), and an area rostralto the SMA (pre-SMA) of two monkeys (Macace fuscata) was examinedduring performance of a trained motor task. The selective NMDAantagonist 0-2-amino-5-phosphonovaleric acid (APV) and the non-NMDAantagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) wereiontophoretically applied to motor task-related neurons. A totalof 568 task-related neurons (435 movement related, 83 set related,50 mixed type) were recorded from the MI, PM, SMA, and pre-SMA,and the effects of APV and CNQX were examined in the individualneurons. In many neurons, APV selectively or preferentiallysuppressed the spontaneous discharge rather than movement-relatedactivity. In many neurons, the movement-related activity wasmore selectively or effectively suppressed by CNQX than by APV.However, the set-related activity was affected by both APV endCNQX. The neurons in layers I and II were affected more stronglyby APV end CNQX than those in layers V and VI. No correlationwas found between the magnitude of task-related activity inthe control (no drug application) period and the effectivenessof APV or CNOX. These results indicate that both NMDA and non-NMDAglutamate receptors are involved in motor task-related neuronalactivity of both primary and secondary motor areas, althoughthe contribution of these two receptors to individual neuronalactivity varies a great deal.  相似文献   
63.
We investigated the relative effects of 0.5, 1.0, 1.5, 2.0 MAC halothane and enflurane, and concurrent noxious stimulus on hepatic blood flow and oxygen consumption in 14 mongrel dogs randomly divided into groups of seven each. Hepatic arterial and portal venous blood flow (HABF and PVBF, respectively) were measured continuously using ultrasonic transit time flow meter. Mean arterial blood pressure (MAP), cardiac index (CI), hepatic oxygen supply, and hepatic oxygen consumption (H O 2) were measured. Halothane significantly deceased HABF, but not PVBF in a dose dependent manner. Enflurane did not affect HABF and PVBF significantly. MAP and CI decreased in both groups, with halothane producing more marked decreases than enflurane. H O 2 did not change with enflurane, but did with halothane, producing significant differences, with halothane being greater at 1.5, 2.0 MAC. A noxious stimulus only caused minor change in blood flow. The results suggest that liver blood flow and oxygen consumption are affected differently by halothane and enflurane and that halothane has a stronger tendency to cause an imbalance between liver oxygen supply and consumption than dose enflurane.(Masaki E, Yasuda N, Tanifuji Y et al.: Effect of halothane and enflurane on hepatic blood flow and oxygen consumption in dogs. J Anesth 3: 118–122, 1989)  相似文献   
64.
65.
The gene Bcl11b, which encodes zinc finger proteins, and its paralog, Bcl11a, are associated with immune-system malignancies. We have generated Bcl11b-deficient mice that show a block at the CD4-CD8- double-negative stage of thymocyte development without any impairment in cells of B- or gammadelta T cell lineages. The Bcl11b-/- thymocytes showed unsuccessful recombination of V(beta) to D(beta) and lacked the pre-T cell receptor (TCR) complex on the cell surface, owing to the absence of Tcrb mRNA expression. In addition, we saw profound apoptosis in the thymus of neonatal Bcl11b-/- mice. These results suggest that Bcl11b is a key regulator of both differentiation and survival during thymocyte development.  相似文献   
66.
Polysialic acid is a developmentally regulated carbohydrate composed of a linear homopolymer of a-2,a-linked sialic acid residues. This unique glycan is mainly attached to the neural cell adhesion molecule (N-CAM) and implicated in many morphogenic events of the neural cells by modulating the adhesive property of N-CAM. Recently, the cDNA that encodes polysialyltransferase, which is responsible for the polysialylation of N-CAM, was successfully cloned from three mammalian species. This review focuses on the molecular cloning of human polysialyltransferase, designated PST. it then describes the number of enzymes actually required for the polysialylation of N-CAM using an in vitro polysialyltransferase assay. Comparisons between PST and another polysialyltransferase, sialyltransferase X (STX), are made and it Is demonstrated that both enzymes can independently form polysiatic acid In vitro , but that during neural development they coordinately but distinctly synthesize polysialic acid on N-CAM. The role of polysialic acid in the central nervous system is also discussed. Finally, evidence that the two polysialyltransferases, PST and STX, apparently have distinct roles in the development of neural cells is provided by using a neurite outgrowth assay.  相似文献   
67.
Temperature-dependent ESR spectra of Cu2+-Cu2+ pairs in ethylene/methacrylic acid copolymer neutralized with Cu(II) were reexamined in detail. The resonance positions and the linewidths of one of the ESR fine-structure lines showed thermal distension of the Cu2+-Cu2+ distance, and the slopes in the temperature variations changed at the temperature associated with melting of the polymer crystallites. No meaningful anomalies were observed around the temperature at which the preceding endothermic transition takes place. In this transition, the Cu2+-Cu2+ pairs seems to enter a disordered state, keeping almost the same paired structure. In contrast to this irreversible order-disorder transition, the melting process in the most part of the polyethylene crystallite phases starts to impose stress upon the Cu2+-Cu2+ pairs, accompanying the slope changes of the ESR parameters. These reversible variations with remarkable thermal hysteresis are compatible with the DSC analyses.  相似文献   
68.
69.
A genomic subtraction between a pandemic Vibrio parahaemolyticus and a nonpandemic strain that seemed to be clonally related was performed. A subtractive DNA fragment was identified to be a part of a 16-kbp insertion sequence which was present in almost all pandemic strains but not in nonpandemic strains tested.  相似文献   
70.
应用微阵列初步分析髓母细胞瘤的基因表达谱   总被引:10,自引:0,他引:10  
目的 应用微阵列技术研究髓母细胞瘤的分子发病机理。方法 收集新鲜髓母细胞瘤 4例及正常脑组织 1份的组织标本 ,提取总 RNA,逆转录成 32 P标记的 c DNA探针 ,与 Atlas人肿瘤芯片杂交 ,通过放射自显影获得基因谱 ,应用 Atlas Image TM1.0 1a分析。结果 与正常脑组织相比 ,髓母细胞瘤下调基因 6个 ,上调基因 35个 ;逆转录 -聚合酶链反应技术验证结果与芯片检测结果相符。除少数基因外 ,大部分基因的表达趋势与肿瘤生物学特性相符。结论 髓母细胞瘤是与星形细胞起源胶质瘤具有不同分子发病机理的多基因病变 ,不同基因之间可能存在复杂的相互作用和联系 ,值得进一步研究。  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号